One-step concentration and partial purification of <i>Aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfilters

Autores
Voget, Claudio Enrique; Vita, Carolina Elena; Contreras Esquivel, Juan Carlos
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The non-acidic polygalacturonases produced by Aspergillus kawachii in a glucose/tryptone medium were adsorbed to a glass fiber microfilter that was used to clarify the fermentation broth. Maximum adsorption occurred at pH 3 under low ionic strength conditions. The adsorbed activity could be readily released with a buffer solution at pH 5. Based upon these observations, a separation process was developed which enabled the broth to be clarified and, at the same time, the non-acidic polygalacturonases to be concentrated 20-fold and purified 100-fold in a unique filtration step. The practical advantage of recovering polygalacturonases by a filtration process lies in the simplicity and efficiency of the operation involved.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Ciencias Exactas
Química
Aspergillus kawachii
glass fiber microfilter
polygalacturonases
protein adsorption
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/145008
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/145008
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling One-step concentration and partial purification of <i>Aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfiltersVoget, Claudio EnriqueVita, Carolina ElenaContreras Esquivel, Juan CarlosCiencias ExactasQuímicaAspergillus kawachiiglass fiber microfilterpolygalacturonasesprotein adsorptionThe non-acidic polygalacturonases produced by <i>Aspergillus kawachii</i> in a glucose/tryptone medium were adsorbed to a glass fiber microfilter that was used to clarify the fermentation broth. Maximum adsorption occurred at pH 3 under low ionic strength conditions. The adsorbed activity could be readily released with a buffer solution at pH 5. Based upon these observations, a separation process was developed which enabled the broth to be clarified and, at the same time, the non-acidic polygalacturonases to be concentrated 20-fold and purified 100-fold in a unique filtration step. The practical advantage of recovering polygalacturonases by a filtration process lies in the simplicity and efficiency of the operation involved.Centro de Investigación y Desarrollo en Fermentaciones Industriales2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf233-239http://sedici.unlp.edu.ar/handle/10915/145008enginfo:eu-repo/semantics/altIdentifier/issn/0141-5492info:eu-repo/semantics/altIdentifier/issn/1573-6776info:eu-repo/semantics/altIdentifier/doi/10.1007/s10529-005-5524-1info:eu-repo/semantics/altIdentifier/pmid/16555006info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:27Zoai:sedici.unlp.edu.ar:10915/145008Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:28.222SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv One-step concentration and partial purification of <i>Aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfilters
title One-step concentration and partial purification of <i>Aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfilters
spellingShingle One-step concentration and partial purification of <i>Aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfilters
Voget, Claudio Enrique
Ciencias Exactas
Química
Aspergillus kawachii
glass fiber microfilter
polygalacturonases
protein adsorption
title_short One-step concentration and partial purification of <i>Aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfilters
title_full One-step concentration and partial purification of <i>Aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfilters
title_fullStr One-step concentration and partial purification of <i>Aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfilters
title_full_unstemmed One-step concentration and partial purification of <i>Aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfilters
title_sort One-step concentration and partial purification of <i>Aspergillus kawachii</i> non-acidic polygalacturonases by adsorption to glass fiber microfilters
dc.creator.none.fl_str_mv Voget, Claudio Enrique
Vita, Carolina Elena
Contreras Esquivel, Juan Carlos
author Voget, Claudio Enrique
author_facet Voget, Claudio Enrique
Vita, Carolina Elena
Contreras Esquivel, Juan Carlos
author_role author
author2 Vita, Carolina Elena
Contreras Esquivel, Juan Carlos
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Química
Aspergillus kawachii
glass fiber microfilter
polygalacturonases
protein adsorption
topic Ciencias Exactas
Química
Aspergillus kawachii
glass fiber microfilter
polygalacturonases
protein adsorption
dc.description.none.fl_txt_mv The non-acidic polygalacturonases produced by <i>Aspergillus kawachii</i> in a glucose/tryptone medium were adsorbed to a glass fiber microfilter that was used to clarify the fermentation broth. Maximum adsorption occurred at pH 3 under low ionic strength conditions. The adsorbed activity could be readily released with a buffer solution at pH 5. Based upon these observations, a separation process was developed which enabled the broth to be clarified and, at the same time, the non-acidic polygalacturonases to be concentrated 20-fold and purified 100-fold in a unique filtration step. The practical advantage of recovering polygalacturonases by a filtration process lies in the simplicity and efficiency of the operation involved.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description The non-acidic polygalacturonases produced by <i>Aspergillus kawachii</i> in a glucose/tryptone medium were adsorbed to a glass fiber microfilter that was used to clarify the fermentation broth. Maximum adsorption occurred at pH 3 under low ionic strength conditions. The adsorbed activity could be readily released with a buffer solution at pH 5. Based upon these observations, a separation process was developed which enabled the broth to be clarified and, at the same time, the non-acidic polygalacturonases to be concentrated 20-fold and purified 100-fold in a unique filtration step. The practical advantage of recovering polygalacturonases by a filtration process lies in the simplicity and efficiency of the operation involved.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/145008
url http://sedici.unlp.edu.ar/handle/10915/145008
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0141-5492
info:eu-repo/semantics/altIdentifier/issn/1573-6776
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10529-005-5524-1
info:eu-repo/semantics/altIdentifier/pmid/16555006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
233-239
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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