Experimental set up for the irradiation of biological samples and nuclear track detectors with UV C

Autores
Portu, Agustina Mariana; Rossini, Andrés Eugenio; Gadan, Mario Alberto; Bernaola, Omar Alberto; Thorp, Silvia Inés; Curotto, Paula; Pozzi, Emiliano César Cayetano; Cabrini, Romulo Luis; Saint Martin, María Laura Gisela
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aim: In this work we present a methodology to produce an ?imprint? of cells cultivated on a polycarbonate detector by exposure of the detector to UV C radiation.Background: The distribution and concentration of10B atoms in tissue samples coming from BNCT (Boron Neutron Capture Therapy) protocols can be determined through the quantification and analysis of the tracks forming its autoradiography image on a nuclear track detector. The location of boron atoms in the cell structure could be known more accurately by the simultaneous observation of the nuclear tracks and the sample image on the detector.Materials and Methods: A UV C irradiator was constructed. The irradiance was measured along the lamp direction and at different distances. Melanoma cells were cultured on poly-carbonate foils, incubated with borophenylalanine, irradiated with thermal neutrons and exposed to UV C radiation. The samples were chemically attacked with a KOH solution.Results: A uniform irradiation field was established to expose the detector foils to UV C light.Cells could be seeded on the polycarbonate surface. Both imprints from cells and nuclear tracks were obtained after chemical etching.
Fil: Portu, Agustina Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina
Fil: Rossini, Andrés Eugenio. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gadan, Mario Alberto. Comisión Nacional de Energía Atómica; Argentina
Fil: Bernaola, Omar Alberto. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina
Fil: Thorp, Silvia Inés. Comisión Nacional de Energía Atómica; Argentina
Fil: Curotto, Paula. Comisión Nacional de Energía Atómica; Argentina
Fil: Pozzi, Emiliano César Cayetano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Cabrini, Romulo Luis. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina
Fil: Saint Martin, María Laura Gisela. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
BNCT
CELL IMPRINTS
POLYCARBONATE DETECTORS
UV-C
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/113880
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/113880
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Experimental set up for the irradiation of biological samples and nuclear track detectors with UV CPortu, Agustina MarianaRossini, Andrés EugenioGadan, Mario AlbertoBernaola, Omar AlbertoThorp, Silvia InésCurotto, PaulaPozzi, Emiliano César CayetanoCabrini, Romulo LuisSaint Martin, María Laura GiselaBNCTCELL IMPRINTSPOLYCARBONATE DETECTORSUV-Chttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1Aim: In this work we present a methodology to produce an ?imprint? of cells cultivated on a polycarbonate detector by exposure of the detector to UV C radiation.Background: The distribution and concentration of10B atoms in tissue samples coming from BNCT (Boron Neutron Capture Therapy) protocols can be determined through the quantification and analysis of the tracks forming its autoradiography image on a nuclear track detector. The location of boron atoms in the cell structure could be known more accurately by the simultaneous observation of the nuclear tracks and the sample image on the detector.Materials and Methods: A UV C irradiator was constructed. The irradiance was measured along the lamp direction and at different distances. Melanoma cells were cultured on poly-carbonate foils, incubated with borophenylalanine, irradiated with thermal neutrons and exposed to UV C radiation. The samples were chemically attacked with a KOH solution.Results: A uniform irradiation field was established to expose the detector foils to UV C light.Cells could be seeded on the polycarbonate surface. Both imprints from cells and nuclear tracks were obtained after chemical etching.Fil: Portu, Agustina Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); ArgentinaFil: Rossini, Andrés Eugenio. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gadan, Mario Alberto. Comisión Nacional de Energía Atómica; ArgentinaFil: Bernaola, Omar Alberto. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); ArgentinaFil: Thorp, Silvia Inés. Comisión Nacional de Energía Atómica; ArgentinaFil: Curotto, Paula. Comisión Nacional de Energía Atómica; ArgentinaFil: Pozzi, Emiliano César Cayetano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Cabrini, Romulo Luis. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); ArgentinaFil: Saint Martin, María Laura Gisela. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2016-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113880Portu, Agustina Mariana; Rossini, Andrés Eugenio; Gadan, Mario Alberto; Bernaola, Omar Alberto; Thorp, Silvia Inés; et al.; Experimental set up for the irradiation of biological samples and nuclear track detectors with UV C; Elsevier; Reports of Practical Oncology and Radiotherapy; 21; 2; 3-2016; 129-1341507-1367CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1507136714001680info:eu-repo/semantics/altIdentifier/doi/10.1016/j.rpor.2014.10.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:42:13Zoai:ri.conicet.gov.ar:11336/113880instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:42:13.671CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Experimental set up for the irradiation of biological samples and nuclear track detectors with UV C
title Experimental set up for the irradiation of biological samples and nuclear track detectors with UV C
spellingShingle Experimental set up for the irradiation of biological samples and nuclear track detectors with UV C
Portu, Agustina Mariana
BNCT
CELL IMPRINTS
POLYCARBONATE DETECTORS
UV-C
title_short Experimental set up for the irradiation of biological samples and nuclear track detectors with UV C
title_full Experimental set up for the irradiation of biological samples and nuclear track detectors with UV C
title_fullStr Experimental set up for the irradiation of biological samples and nuclear track detectors with UV C
title_full_unstemmed Experimental set up for the irradiation of biological samples and nuclear track detectors with UV C
title_sort Experimental set up for the irradiation of biological samples and nuclear track detectors with UV C
dc.creator.none.fl_str_mv Portu, Agustina Mariana
Rossini, Andrés Eugenio
Gadan, Mario Alberto
Bernaola, Omar Alberto
Thorp, Silvia Inés
Curotto, Paula
Pozzi, Emiliano César Cayetano
Cabrini, Romulo Luis
Saint Martin, María Laura Gisela
author Portu, Agustina Mariana
author_facet Portu, Agustina Mariana
Rossini, Andrés Eugenio
Gadan, Mario Alberto
Bernaola, Omar Alberto
Thorp, Silvia Inés
Curotto, Paula
Pozzi, Emiliano César Cayetano
Cabrini, Romulo Luis
Saint Martin, María Laura Gisela
author_role author
author2 Rossini, Andrés Eugenio
Gadan, Mario Alberto
Bernaola, Omar Alberto
Thorp, Silvia Inés
Curotto, Paula
Pozzi, Emiliano César Cayetano
Cabrini, Romulo Luis
Saint Martin, María Laura Gisela
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BNCT
CELL IMPRINTS
POLYCARBONATE DETECTORS
UV-C
topic BNCT
CELL IMPRINTS
POLYCARBONATE DETECTORS
UV-C
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aim: In this work we present a methodology to produce an ?imprint? of cells cultivated on a polycarbonate detector by exposure of the detector to UV C radiation.Background: The distribution and concentration of10B atoms in tissue samples coming from BNCT (Boron Neutron Capture Therapy) protocols can be determined through the quantification and analysis of the tracks forming its autoradiography image on a nuclear track detector. The location of boron atoms in the cell structure could be known more accurately by the simultaneous observation of the nuclear tracks and the sample image on the detector.Materials and Methods: A UV C irradiator was constructed. The irradiance was measured along the lamp direction and at different distances. Melanoma cells were cultured on poly-carbonate foils, incubated with borophenylalanine, irradiated with thermal neutrons and exposed to UV C radiation. The samples were chemically attacked with a KOH solution.Results: A uniform irradiation field was established to expose the detector foils to UV C light.Cells could be seeded on the polycarbonate surface. Both imprints from cells and nuclear tracks were obtained after chemical etching.
Fil: Portu, Agustina Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina
Fil: Rossini, Andrés Eugenio. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gadan, Mario Alberto. Comisión Nacional de Energía Atómica; Argentina
Fil: Bernaola, Omar Alberto. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina
Fil: Thorp, Silvia Inés. Comisión Nacional de Energía Atómica; Argentina
Fil: Curotto, Paula. Comisión Nacional de Energía Atómica; Argentina
Fil: Pozzi, Emiliano César Cayetano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Cabrini, Romulo Luis. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Anatomía Patológica; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina
Fil: Saint Martin, María Laura Gisela. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Aim: In this work we present a methodology to produce an ?imprint? of cells cultivated on a polycarbonate detector by exposure of the detector to UV C radiation.Background: The distribution and concentration of10B atoms in tissue samples coming from BNCT (Boron Neutron Capture Therapy) protocols can be determined through the quantification and analysis of the tracks forming its autoradiography image on a nuclear track detector. The location of boron atoms in the cell structure could be known more accurately by the simultaneous observation of the nuclear tracks and the sample image on the detector.Materials and Methods: A UV C irradiator was constructed. The irradiance was measured along the lamp direction and at different distances. Melanoma cells were cultured on poly-carbonate foils, incubated with borophenylalanine, irradiated with thermal neutrons and exposed to UV C radiation. The samples were chemically attacked with a KOH solution.Results: A uniform irradiation field was established to expose the detector foils to UV C light.Cells could be seeded on the polycarbonate surface. Both imprints from cells and nuclear tracks were obtained after chemical etching.
publishDate 2016
dc.date.none.fl_str_mv 2016-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/113880
Portu, Agustina Mariana; Rossini, Andrés Eugenio; Gadan, Mario Alberto; Bernaola, Omar Alberto; Thorp, Silvia Inés; et al.; Experimental set up for the irradiation of biological samples and nuclear track detectors with UV C; Elsevier; Reports of Practical Oncology and Radiotherapy; 21; 2; 3-2016; 129-134
1507-1367
CONICET Digital
CONICET
url http://hdl.handle.net/11336/113880
identifier_str_mv Portu, Agustina Mariana; Rossini, Andrés Eugenio; Gadan, Mario Alberto; Bernaola, Omar Alberto; Thorp, Silvia Inés; et al.; Experimental set up for the irradiation of biological samples and nuclear track detectors with UV C; Elsevier; Reports of Practical Oncology and Radiotherapy; 21; 2; 3-2016; 129-134
1507-1367
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1507136714001680
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.rpor.2014.10.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082922778460160
score 13.22299

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