Neutron Autoradiography Combined With UV-C Sensitization: Toward the Intracellular Localization of Boron

Autores
Gadan, Mario Alberto; Lloyd, Rodrigo; Saint Martin, María Laura Gisela; Olivera, María S.; Policastro, Lucia Laura; Portu, Agustina Mariana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Our group has reported the imprint formation of biological material on polycarbonate nuclear track detectors by UV-C exposure, which is used as an approach to simultaneously visualize cell imprints and nuclear tracks coming from the boron neutron capture reaction. Considering that the cell nucleus has a higher UV-C absorption than the cytoplasm and that hematoxylin preferentially stains the nucleus, we proposed to enhance the contrast between these two main cell structures by hematoxylin staining before UV-C sensitization. In this study, several experiments were performed in order to optimize UV-C exposure parameters and chemical etching conditions for cell imprint formation using the SK-BR-3 breast cancer cell line. The proposed method improves significantly the resolution of the cell imprints. It allows clear differentiation of the nucleus from the rest of the cell, together with nuclear tracks pits. Moreover, it reduces considerably the UV-C exposure time, an important experimental issue. The proposed methodology can be applied to study the boron distribution independently from the chosen cell line and/or boron compounds.
Fil: Gadan, Mario Alberto. Comisión Nacional de Energía Atómica; Argentina
Fil: Lloyd, Rodrigo. Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Saint Martin, María Laura Gisela. Comisión Nacional de Energía Atómica; Argentina
Fil: Olivera, María S.. Comisión Nacional de Energía Atómica; Argentina
Fil: Policastro, Lucia Laura. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia - Nodo Constituyentes | Comision Nacional de Energia Atomica. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia - Nodo Constituyentes.; Argentina
Fil: Portu, Agustina Mariana. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
BORON MICRODISTRIBUTION
BORON NEUTRON CAPTURE THERAPY (BNCT)
NEUTRON AUTORADIOGRAPHY
POLYCARBONATE NUCLEAR TRACK DETECTOR
UV-C SENSITIZATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/123844
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/123844
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neutron Autoradiography Combined With UV-C Sensitization: Toward the Intracellular Localization of BoronGadan, Mario AlbertoLloyd, RodrigoSaint Martin, María Laura GiselaOlivera, María S.Policastro, Lucia LauraPortu, Agustina MarianaBORON MICRODISTRIBUTIONBORON NEUTRON CAPTURE THERAPY (BNCT)NEUTRON AUTORADIOGRAPHYPOLYCARBONATE NUCLEAR TRACK DETECTORUV-C SENSITIZATIONhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1Our group has reported the imprint formation of biological material on polycarbonate nuclear track detectors by UV-C exposure, which is used as an approach to simultaneously visualize cell imprints and nuclear tracks coming from the boron neutron capture reaction. Considering that the cell nucleus has a higher UV-C absorption than the cytoplasm and that hematoxylin preferentially stains the nucleus, we proposed to enhance the contrast between these two main cell structures by hematoxylin staining before UV-C sensitization. In this study, several experiments were performed in order to optimize UV-C exposure parameters and chemical etching conditions for cell imprint formation using the SK-BR-3 breast cancer cell line. The proposed method improves significantly the resolution of the cell imprints. It allows clear differentiation of the nucleus from the rest of the cell, together with nuclear tracks pits. Moreover, it reduces considerably the UV-C exposure time, an important experimental issue. The proposed methodology can be applied to study the boron distribution independently from the chosen cell line and/or boron compounds.Fil: Gadan, Mario Alberto. Comisión Nacional de Energía Atómica; ArgentinaFil: Lloyd, Rodrigo. Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Saint Martin, María Laura Gisela. Comisión Nacional de Energía Atómica; ArgentinaFil: Olivera, María S.. Comisión Nacional de Energía Atómica; ArgentinaFil: Policastro, Lucia Laura. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia - Nodo Constituyentes | Comision Nacional de Energia Atomica. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia - Nodo Constituyentes.; ArgentinaFil: Portu, Agustina Mariana. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaCambridge University Press2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/123844Gadan, Mario Alberto; Lloyd, Rodrigo; Saint Martin, María Laura Gisela; Olivera, María S.; Policastro, Lucia Laura; et al.; Neutron Autoradiography Combined With UV-C Sensitization: Toward the Intracellular Localization of Boron; Cambridge University Press; Microscopy & Microanalysis; 25; 6; 10-2019; 1331-13401431-9276CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/product/identifier/S1431927619015058/type/journal_articleinfo:eu-repo/semantics/altIdentifier/doi/10.1017/S1431927619015058info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:33Zoai:ri.conicet.gov.ar:11336/123844instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:33.309CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neutron Autoradiography Combined With UV-C Sensitization: Toward the Intracellular Localization of Boron
title Neutron Autoradiography Combined With UV-C Sensitization: Toward the Intracellular Localization of Boron
spellingShingle Neutron Autoradiography Combined With UV-C Sensitization: Toward the Intracellular Localization of Boron
Gadan, Mario Alberto
BORON MICRODISTRIBUTION
BORON NEUTRON CAPTURE THERAPY (BNCT)
NEUTRON AUTORADIOGRAPHY
POLYCARBONATE NUCLEAR TRACK DETECTOR
UV-C SENSITIZATION
title_short Neutron Autoradiography Combined With UV-C Sensitization: Toward the Intracellular Localization of Boron
title_full Neutron Autoradiography Combined With UV-C Sensitization: Toward the Intracellular Localization of Boron
title_fullStr Neutron Autoradiography Combined With UV-C Sensitization: Toward the Intracellular Localization of Boron
title_full_unstemmed Neutron Autoradiography Combined With UV-C Sensitization: Toward the Intracellular Localization of Boron
title_sort Neutron Autoradiography Combined With UV-C Sensitization: Toward the Intracellular Localization of Boron
dc.creator.none.fl_str_mv Gadan, Mario Alberto
Lloyd, Rodrigo
Saint Martin, María Laura Gisela
Olivera, María S.
Policastro, Lucia Laura
Portu, Agustina Mariana
author Gadan, Mario Alberto
author_facet Gadan, Mario Alberto
Lloyd, Rodrigo
Saint Martin, María Laura Gisela
Olivera, María S.
Policastro, Lucia Laura
Portu, Agustina Mariana
author_role author
author2 Lloyd, Rodrigo
Saint Martin, María Laura Gisela
Olivera, María S.
Policastro, Lucia Laura
Portu, Agustina Mariana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BORON MICRODISTRIBUTION
BORON NEUTRON CAPTURE THERAPY (BNCT)
NEUTRON AUTORADIOGRAPHY
POLYCARBONATE NUCLEAR TRACK DETECTOR
UV-C SENSITIZATION
topic BORON MICRODISTRIBUTION
BORON NEUTRON CAPTURE THERAPY (BNCT)
NEUTRON AUTORADIOGRAPHY
POLYCARBONATE NUCLEAR TRACK DETECTOR
UV-C SENSITIZATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Our group has reported the imprint formation of biological material on polycarbonate nuclear track detectors by UV-C exposure, which is used as an approach to simultaneously visualize cell imprints and nuclear tracks coming from the boron neutron capture reaction. Considering that the cell nucleus has a higher UV-C absorption than the cytoplasm and that hematoxylin preferentially stains the nucleus, we proposed to enhance the contrast between these two main cell structures by hematoxylin staining before UV-C sensitization. In this study, several experiments were performed in order to optimize UV-C exposure parameters and chemical etching conditions for cell imprint formation using the SK-BR-3 breast cancer cell line. The proposed method improves significantly the resolution of the cell imprints. It allows clear differentiation of the nucleus from the rest of the cell, together with nuclear tracks pits. Moreover, it reduces considerably the UV-C exposure time, an important experimental issue. The proposed methodology can be applied to study the boron distribution independently from the chosen cell line and/or boron compounds.
Fil: Gadan, Mario Alberto. Comisión Nacional de Energía Atómica; Argentina
Fil: Lloyd, Rodrigo. Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Saint Martin, María Laura Gisela. Comisión Nacional de Energía Atómica; Argentina
Fil: Olivera, María S.. Comisión Nacional de Energía Atómica; Argentina
Fil: Policastro, Lucia Laura. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia - Nodo Constituyentes | Comision Nacional de Energia Atomica. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia. Unidad Ejecutora Instituto de Nanociencia y Nanotecnologia - Nodo Constituyentes.; Argentina
Fil: Portu, Agustina Mariana. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Our group has reported the imprint formation of biological material on polycarbonate nuclear track detectors by UV-C exposure, which is used as an approach to simultaneously visualize cell imprints and nuclear tracks coming from the boron neutron capture reaction. Considering that the cell nucleus has a higher UV-C absorption than the cytoplasm and that hematoxylin preferentially stains the nucleus, we proposed to enhance the contrast between these two main cell structures by hematoxylin staining before UV-C sensitization. In this study, several experiments were performed in order to optimize UV-C exposure parameters and chemical etching conditions for cell imprint formation using the SK-BR-3 breast cancer cell line. The proposed method improves significantly the resolution of the cell imprints. It allows clear differentiation of the nucleus from the rest of the cell, together with nuclear tracks pits. Moreover, it reduces considerably the UV-C exposure time, an important experimental issue. The proposed methodology can be applied to study the boron distribution independently from the chosen cell line and/or boron compounds.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/123844
Gadan, Mario Alberto; Lloyd, Rodrigo; Saint Martin, María Laura Gisela; Olivera, María S.; Policastro, Lucia Laura; et al.; Neutron Autoradiography Combined With UV-C Sensitization: Toward the Intracellular Localization of Boron; Cambridge University Press; Microscopy & Microanalysis; 25; 6; 10-2019; 1331-1340
1431-9276
CONICET Digital
CONICET
url http://hdl.handle.net/11336/123844
identifier_str_mv Gadan, Mario Alberto; Lloyd, Rodrigo; Saint Martin, María Laura Gisela; Olivera, María S.; Policastro, Lucia Laura; et al.; Neutron Autoradiography Combined With UV-C Sensitization: Toward the Intracellular Localization of Boron; Cambridge University Press; Microscopy & Microanalysis; 25; 6; 10-2019; 1331-1340
1431-9276
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/product/identifier/S1431927619015058/type/journal_article
info:eu-repo/semantics/altIdentifier/doi/10.1017/S1431927619015058
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268979431735296
score 13.13397

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