Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models

Autores
Portu, Agustina Mariana; Espain, Maria Sol; Thorp, Silvia Inés; Trivillin, Verónica Andrea; Curotto, Paula; Monti Hughes, Andrea; Pozzi, Emiliano César Cayetano; Garabalino, Marcela Alejandra; Palmieri, Mónica Alejandra; Granell, Pablo Nicolás; Golmar, Federico; Schwint, Amanda Elena; Saint Martin, Gisela
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The assessment of boron microdistribution is essential to evaluate the suitability of boron neutron capture therapy (BNCT) in different biological models. In our laboratory, we have reported a methodology to produce cell imprints on polycarbonate through UV-C sensitization. The aim of this work is to extend the technique to tissue samples in order to enhance spatial resolution. As tissue structure largely differs from cultured cells, several aspects must be considered. We studied the influence of the parameters involved in the imprint and nuclear track formation, such as neutron fluence, different NTDs, etching and UV-C exposure times, tissue absorbance, thickness, and staining, among others. Samples from different biological models of interest for BNCT were used, exhibiting homogeneous and heterogeneous histology and boron microdistribution. The optimal conditions will depend on the animal model under study and the resolution requirements. Both the imprint sharpness and the fading effect depend on tissue thickness. While 6 h of UV-C was necessary to yield an imprint in CR-39, only 5 min was enough to observe clear imprints on Lexan. The information related to microdistribution of boron obtained with neutron autoradiography is of great relevance when assessing new boron compounds and administration protocols and also contributes to the study of the radiobiology of BNCT.
Fil: Portu, Agustina Mariana. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Espain, Maria Sol. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Thorp, Silvia Inés. Comisión Nacional de Energía Atómica; Argentina
Fil: Trivillin, Verónica Andrea. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Curotto, Paula. Comisión Nacional de Energía Atómica; Argentina
Fil: Monti Hughes, Andrea. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pozzi, Emiliano César Cayetano. Comisión Nacional de Energía Atómica; Argentina
Fil: Garabalino, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palmieri, Mónica Alejandra. Universidad de Buenos Aires; Argentina
Fil: Granell, Pablo Nicolás. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Golmar, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Schwint, Amanda Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Saint Martin, Gisela. Comisión Nacional de Energía Atómica; Argentina
Materia
ANIMAL MODELS
BNCT
BORON MICRODISTRIBUTION
NEUTRON AUTORADIOGRAPHY
NUCLEAR TRACK DETECTORS
PADC
POLYCARBONATE
UV-C
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/219916
Acceder
id CONICETDig_3d420bb125dbbb9c39a69c98f962616b
oai_identifier_str oai:ri.conicet.gov.ar:11336/219916
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal ModelsPortu, Agustina MarianaEspain, Maria SolThorp, Silvia InésTrivillin, Verónica AndreaCurotto, PaulaMonti Hughes, AndreaPozzi, Emiliano César CayetanoGarabalino, Marcela AlejandraPalmieri, Mónica AlejandraGranell, Pablo NicolásGolmar, FedericoSchwint, Amanda ElenaSaint Martin, GiselaANIMAL MODELSBNCTBORON MICRODISTRIBUTIONNEUTRON AUTORADIOGRAPHYNUCLEAR TRACK DETECTORSPADCPOLYCARBONATEUV-Chttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1The assessment of boron microdistribution is essential to evaluate the suitability of boron neutron capture therapy (BNCT) in different biological models. In our laboratory, we have reported a methodology to produce cell imprints on polycarbonate through UV-C sensitization. The aim of this work is to extend the technique to tissue samples in order to enhance spatial resolution. As tissue structure largely differs from cultured cells, several aspects must be considered. We studied the influence of the parameters involved in the imprint and nuclear track formation, such as neutron fluence, different NTDs, etching and UV-C exposure times, tissue absorbance, thickness, and staining, among others. Samples from different biological models of interest for BNCT were used, exhibiting homogeneous and heterogeneous histology and boron microdistribution. The optimal conditions will depend on the animal model under study and the resolution requirements. Both the imprint sharpness and the fading effect depend on tissue thickness. While 6 h of UV-C was necessary to yield an imprint in CR-39, only 5 min was enough to observe clear imprints on Lexan. The information related to microdistribution of boron obtained with neutron autoradiography is of great relevance when assessing new boron compounds and administration protocols and also contributes to the study of the radiobiology of BNCT.Fil: Portu, Agustina Mariana. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Espain, Maria Sol. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Thorp, Silvia Inés. Comisión Nacional de Energía Atómica; ArgentinaFil: Trivillin, Verónica Andrea. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Curotto, Paula. Comisión Nacional de Energía Atómica; ArgentinaFil: Monti Hughes, Andrea. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pozzi, Emiliano César Cayetano. Comisión Nacional de Energía Atómica; ArgentinaFil: Garabalino, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Palmieri, Mónica Alejandra. Universidad de Buenos Aires; ArgentinaFil: Granell, Pablo Nicolás. Instituto Nacional de Tecnología Industrial; ArgentinaFil: Golmar, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Schwint, Amanda Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Saint Martin, Gisela. Comisión Nacional de Energía Atómica; ArgentinaMDPI2023-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/219916Portu, Agustina Mariana; Espain, Maria Sol; Thorp, Silvia Inés; Trivillin, Verónica Andrea; Curotto, Paula; et al.; Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models; MDPI; Life; 13; 7; 7-2023; 1-222075-1729CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2075-1729/13/7/1578info:eu-repo/semantics/altIdentifier/doi/10.3390/life13071578info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:51Zoai:ri.conicet.gov.ar:11336/219916instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:52.042CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models
title Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models
spellingShingle Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models
Portu, Agustina Mariana
ANIMAL MODELS
BNCT
BORON MICRODISTRIBUTION
NEUTRON AUTORADIOGRAPHY
NUCLEAR TRACK DETECTORS
PADC
POLYCARBONATE
UV-C
title_short Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models
title_full Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models
title_fullStr Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models
title_full_unstemmed Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models
title_sort Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models
dc.creator.none.fl_str_mv Portu, Agustina Mariana
Espain, Maria Sol
Thorp, Silvia Inés
Trivillin, Verónica Andrea
Curotto, Paula
Monti Hughes, Andrea
Pozzi, Emiliano César Cayetano
Garabalino, Marcela Alejandra
Palmieri, Mónica Alejandra
Granell, Pablo Nicolás
Golmar, Federico
Schwint, Amanda Elena
Saint Martin, Gisela
author Portu, Agustina Mariana
author_facet Portu, Agustina Mariana
Espain, Maria Sol
Thorp, Silvia Inés
Trivillin, Verónica Andrea
Curotto, Paula
Monti Hughes, Andrea
Pozzi, Emiliano César Cayetano
Garabalino, Marcela Alejandra
Palmieri, Mónica Alejandra
Granell, Pablo Nicolás
Golmar, Federico
Schwint, Amanda Elena
Saint Martin, Gisela
author_role author
author2 Espain, Maria Sol
Thorp, Silvia Inés
Trivillin, Verónica Andrea
Curotto, Paula
Monti Hughes, Andrea
Pozzi, Emiliano César Cayetano
Garabalino, Marcela Alejandra
Palmieri, Mónica Alejandra
Granell, Pablo Nicolás
Golmar, Federico
Schwint, Amanda Elena
Saint Martin, Gisela
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANIMAL MODELS
BNCT
BORON MICRODISTRIBUTION
NEUTRON AUTORADIOGRAPHY
NUCLEAR TRACK DETECTORS
PADC
POLYCARBONATE
UV-C
topic ANIMAL MODELS
BNCT
BORON MICRODISTRIBUTION
NEUTRON AUTORADIOGRAPHY
NUCLEAR TRACK DETECTORS
PADC
POLYCARBONATE
UV-C
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The assessment of boron microdistribution is essential to evaluate the suitability of boron neutron capture therapy (BNCT) in different biological models. In our laboratory, we have reported a methodology to produce cell imprints on polycarbonate through UV-C sensitization. The aim of this work is to extend the technique to tissue samples in order to enhance spatial resolution. As tissue structure largely differs from cultured cells, several aspects must be considered. We studied the influence of the parameters involved in the imprint and nuclear track formation, such as neutron fluence, different NTDs, etching and UV-C exposure times, tissue absorbance, thickness, and staining, among others. Samples from different biological models of interest for BNCT were used, exhibiting homogeneous and heterogeneous histology and boron microdistribution. The optimal conditions will depend on the animal model under study and the resolution requirements. Both the imprint sharpness and the fading effect depend on tissue thickness. While 6 h of UV-C was necessary to yield an imprint in CR-39, only 5 min was enough to observe clear imprints on Lexan. The information related to microdistribution of boron obtained with neutron autoradiography is of great relevance when assessing new boron compounds and administration protocols and also contributes to the study of the radiobiology of BNCT.
Fil: Portu, Agustina Mariana. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Espain, Maria Sol. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Thorp, Silvia Inés. Comisión Nacional de Energía Atómica; Argentina
Fil: Trivillin, Verónica Andrea. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Curotto, Paula. Comisión Nacional de Energía Atómica; Argentina
Fil: Monti Hughes, Andrea. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pozzi, Emiliano César Cayetano. Comisión Nacional de Energía Atómica; Argentina
Fil: Garabalino, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palmieri, Mónica Alejandra. Universidad de Buenos Aires; Argentina
Fil: Granell, Pablo Nicolás. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Golmar, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Schwint, Amanda Elena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Saint Martin, Gisela. Comisión Nacional de Energía Atómica; Argentina
description The assessment of boron microdistribution is essential to evaluate the suitability of boron neutron capture therapy (BNCT) in different biological models. In our laboratory, we have reported a methodology to produce cell imprints on polycarbonate through UV-C sensitization. The aim of this work is to extend the technique to tissue samples in order to enhance spatial resolution. As tissue structure largely differs from cultured cells, several aspects must be considered. We studied the influence of the parameters involved in the imprint and nuclear track formation, such as neutron fluence, different NTDs, etching and UV-C exposure times, tissue absorbance, thickness, and staining, among others. Samples from different biological models of interest for BNCT were used, exhibiting homogeneous and heterogeneous histology and boron microdistribution. The optimal conditions will depend on the animal model under study and the resolution requirements. Both the imprint sharpness and the fading effect depend on tissue thickness. While 6 h of UV-C was necessary to yield an imprint in CR-39, only 5 min was enough to observe clear imprints on Lexan. The information related to microdistribution of boron obtained with neutron autoradiography is of great relevance when assessing new boron compounds and administration protocols and also contributes to the study of the radiobiology of BNCT.
publishDate 2023
dc.date.none.fl_str_mv 2023-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/219916
Portu, Agustina Mariana; Espain, Maria Sol; Thorp, Silvia Inés; Trivillin, Verónica Andrea; Curotto, Paula; et al.; Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models; MDPI; Life; 13; 7; 7-2023; 1-22
2075-1729
CONICET Digital
CONICET
url http://hdl.handle.net/11336/219916
identifier_str_mv Portu, Agustina Mariana; Espain, Maria Sol; Thorp, Silvia Inés; Trivillin, Verónica Andrea; Curotto, Paula; et al.; Enhanced Resolution of Neutron Autoradiography with UV-C Sensitization to Study Boron Microdistribution in Animal Models; MDPI; Life; 13; 7; 7-2023; 1-22
2075-1729
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2075-1729/13/7/1578
info:eu-repo/semantics/altIdentifier/doi/10.3390/life13071578
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269879879598080
score 13.13397

Publicaciones similares