Neutron autoradiography to study boron compound microdistribution in an oral cancer model

Autores
Portu, Agustina Mariana; Molinari, Ana Julia; Thorp, Silvia Inés; Pozzi, Emiliano César Cayetano; Curotto, Paula; Schwint, Amanda Elena; Saint Martin, María Laura Gisela
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Purpose : We previously reported the therapeutic effi cacy of Sequential Boron Neutron Capture Therapy (Seq-BNCT), i.e., BPA (boronophenylalanine) ? BNCT followed by GB-10 (decahydrodecaborate) ? BNCT 1 or 2 days later, in the hamster cheek pouchoral cancer model. We have utilized the neutron autoradiography methodology to study boron microdistribution in tissue.The aim was to use this method to evaluate if the distribution of GB-10 is altered by prior application of BPA-BNCT in Sequential BNCT protocols.Materials and methods : Extensive qualitative and quantitative autoradiography analyses were performed in the following groups: G1 (animals without boron); G2 (animals injected with BPA); G3 (animals injected with GB-10); G4 (same as G3, 24 h after BPA-BNCT); and G5 (same protocol as G4, 48 h interval).Results : A detailed study of boron localization in the diff erent tissue structures of tumor, premalignant and normal tissue in the hamster cheek pouch was performed. GB-10 accumulated preferentially in non-neoplastic connective tissue, whereas for BPA neoplastic cells showed the highest boron concentration. Boron distribution was less heterogeneous for GB-10 than for BPA. In premalignant and normal tissue, GB-10 and BPA accumulatedmostly in connective tissue and epithelium, respectively.Conclusions : BPA-BNCT could alter boron microlocalization of GB-10 administered subsequently. Boron targeting homogeneity is essential for therapeutic success.
Fil: Portu, Agustina Mariana. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Molinari, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina
Fil: Thorp, Silvia Inés. Comisión Nacional de Energía Atómica; Argentina
Fil: Pozzi, Emiliano César Cayetano. Comisión Nacional de Energía Atómica; Argentina
Fil: Curotto, Paula. Comisión Nacional de Energía Atómica; Argentina
Fil: Schwint, Amanda Elena. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Saint Martin, María Laura Gisela. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina
Materia
NEUTRON AUTORADIOGRAPHY
BORON MICRODISTRIBUTION
SEQUENTIAL BNCT
GB-10
BPA
ORAL CANCER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/111429
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/111429
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neutron autoradiography to study boron compound microdistribution in an oral cancer modelPortu, Agustina MarianaMolinari, Ana JuliaThorp, Silvia InésPozzi, Emiliano César CayetanoCurotto, PaulaSchwint, Amanda ElenaSaint Martin, María Laura GiselaNEUTRON AUTORADIOGRAPHYBORON MICRODISTRIBUTIONSEQUENTIAL BNCTGB-10BPAORAL CANCERhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1Purpose : We previously reported the therapeutic effi cacy of Sequential Boron Neutron Capture Therapy (Seq-BNCT), i.e., BPA (boronophenylalanine) ? BNCT followed by GB-10 (decahydrodecaborate) ? BNCT 1 or 2 days later, in the hamster cheek pouchoral cancer model. We have utilized the neutron autoradiography methodology to study boron microdistribution in tissue.The aim was to use this method to evaluate if the distribution of GB-10 is altered by prior application of BPA-BNCT in Sequential BNCT protocols.Materials and methods : Extensive qualitative and quantitative autoradiography analyses were performed in the following groups: G1 (animals without boron); G2 (animals injected with BPA); G3 (animals injected with GB-10); G4 (same as G3, 24 h after BPA-BNCT); and G5 (same protocol as G4, 48 h interval).Results : A detailed study of boron localization in the diff erent tissue structures of tumor, premalignant and normal tissue in the hamster cheek pouch was performed. GB-10 accumulated preferentially in non-neoplastic connective tissue, whereas for BPA neoplastic cells showed the highest boron concentration. Boron distribution was less heterogeneous for GB-10 than for BPA. In premalignant and normal tissue, GB-10 and BPA accumulatedmostly in connective tissue and epithelium, respectively.Conclusions : BPA-BNCT could alter boron microlocalization of GB-10 administered subsequently. Boron targeting homogeneity is essential for therapeutic success.Fil: Portu, Agustina Mariana. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Molinari, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); ArgentinaFil: Thorp, Silvia Inés. Comisión Nacional de Energía Atómica; ArgentinaFil: Pozzi, Emiliano César Cayetano. Comisión Nacional de Energía Atómica; ArgentinaFil: Curotto, Paula. Comisión Nacional de Energía Atómica; ArgentinaFil: Schwint, Amanda Elena. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Saint Martin, María Laura Gisela. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); ArgentinaTaylor & Francis Ltd2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/111429Portu, Agustina Mariana; Molinari, Ana Julia; Thorp, Silvia Inés; Pozzi, Emiliano César Cayetano; Curotto, Paula; et al.; Neutron autoradiography to study boron compound microdistribution in an oral cancer model; Taylor & Francis Ltd; International Journal Of Radiation Biology; 91; 4; 4-2015; 329-3350955-3002CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3109/09553002.2014.995381info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.3109/09553002.2014.995381info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:53Zoai:ri.conicet.gov.ar:11336/111429instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:53.572CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neutron autoradiography to study boron compound microdistribution in an oral cancer model
title Neutron autoradiography to study boron compound microdistribution in an oral cancer model
spellingShingle Neutron autoradiography to study boron compound microdistribution in an oral cancer model
Portu, Agustina Mariana
NEUTRON AUTORADIOGRAPHY
BORON MICRODISTRIBUTION
SEQUENTIAL BNCT
GB-10
BPA
ORAL CANCER
title_short Neutron autoradiography to study boron compound microdistribution in an oral cancer model
title_full Neutron autoradiography to study boron compound microdistribution in an oral cancer model
title_fullStr Neutron autoradiography to study boron compound microdistribution in an oral cancer model
title_full_unstemmed Neutron autoradiography to study boron compound microdistribution in an oral cancer model
title_sort Neutron autoradiography to study boron compound microdistribution in an oral cancer model
dc.creator.none.fl_str_mv Portu, Agustina Mariana
Molinari, Ana Julia
Thorp, Silvia Inés
Pozzi, Emiliano César Cayetano
Curotto, Paula
Schwint, Amanda Elena
Saint Martin, María Laura Gisela
author Portu, Agustina Mariana
author_facet Portu, Agustina Mariana
Molinari, Ana Julia
Thorp, Silvia Inés
Pozzi, Emiliano César Cayetano
Curotto, Paula
Schwint, Amanda Elena
Saint Martin, María Laura Gisela
author_role author
author2 Molinari, Ana Julia
Thorp, Silvia Inés
Pozzi, Emiliano César Cayetano
Curotto, Paula
Schwint, Amanda Elena
Saint Martin, María Laura Gisela
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv NEUTRON AUTORADIOGRAPHY
BORON MICRODISTRIBUTION
SEQUENTIAL BNCT
GB-10
BPA
ORAL CANCER
topic NEUTRON AUTORADIOGRAPHY
BORON MICRODISTRIBUTION
SEQUENTIAL BNCT
GB-10
BPA
ORAL CANCER
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Purpose : We previously reported the therapeutic effi cacy of Sequential Boron Neutron Capture Therapy (Seq-BNCT), i.e., BPA (boronophenylalanine) ? BNCT followed by GB-10 (decahydrodecaborate) ? BNCT 1 or 2 days later, in the hamster cheek pouchoral cancer model. We have utilized the neutron autoradiography methodology to study boron microdistribution in tissue.The aim was to use this method to evaluate if the distribution of GB-10 is altered by prior application of BPA-BNCT in Sequential BNCT protocols.Materials and methods : Extensive qualitative and quantitative autoradiography analyses were performed in the following groups: G1 (animals without boron); G2 (animals injected with BPA); G3 (animals injected with GB-10); G4 (same as G3, 24 h after BPA-BNCT); and G5 (same protocol as G4, 48 h interval).Results : A detailed study of boron localization in the diff erent tissue structures of tumor, premalignant and normal tissue in the hamster cheek pouch was performed. GB-10 accumulated preferentially in non-neoplastic connective tissue, whereas for BPA neoplastic cells showed the highest boron concentration. Boron distribution was less heterogeneous for GB-10 than for BPA. In premalignant and normal tissue, GB-10 and BPA accumulatedmostly in connective tissue and epithelium, respectively.Conclusions : BPA-BNCT could alter boron microlocalization of GB-10 administered subsequently. Boron targeting homogeneity is essential for therapeutic success.
Fil: Portu, Agustina Mariana. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Molinari, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina
Fil: Thorp, Silvia Inés. Comisión Nacional de Energía Atómica; Argentina
Fil: Pozzi, Emiliano César Cayetano. Comisión Nacional de Energía Atómica; Argentina
Fil: Curotto, Paula. Comisión Nacional de Energía Atómica; Argentina
Fil: Schwint, Amanda Elena. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Saint Martin, María Laura Gisela. Comisión Nacional de Energía Atómica. Gerencia de Area de Aplicaciones de la Tecnología Nuclear. Gerencia de Radiobiología (Centro Atómico Constituyentes); Argentina
description Purpose : We previously reported the therapeutic effi cacy of Sequential Boron Neutron Capture Therapy (Seq-BNCT), i.e., BPA (boronophenylalanine) ? BNCT followed by GB-10 (decahydrodecaborate) ? BNCT 1 or 2 days later, in the hamster cheek pouchoral cancer model. We have utilized the neutron autoradiography methodology to study boron microdistribution in tissue.The aim was to use this method to evaluate if the distribution of GB-10 is altered by prior application of BPA-BNCT in Sequential BNCT protocols.Materials and methods : Extensive qualitative and quantitative autoradiography analyses were performed in the following groups: G1 (animals without boron); G2 (animals injected with BPA); G3 (animals injected with GB-10); G4 (same as G3, 24 h after BPA-BNCT); and G5 (same protocol as G4, 48 h interval).Results : A detailed study of boron localization in the diff erent tissue structures of tumor, premalignant and normal tissue in the hamster cheek pouch was performed. GB-10 accumulated preferentially in non-neoplastic connective tissue, whereas for BPA neoplastic cells showed the highest boron concentration. Boron distribution was less heterogeneous for GB-10 than for BPA. In premalignant and normal tissue, GB-10 and BPA accumulatedmostly in connective tissue and epithelium, respectively.Conclusions : BPA-BNCT could alter boron microlocalization of GB-10 administered subsequently. Boron targeting homogeneity is essential for therapeutic success.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/111429
Portu, Agustina Mariana; Molinari, Ana Julia; Thorp, Silvia Inés; Pozzi, Emiliano César Cayetano; Curotto, Paula; et al.; Neutron autoradiography to study boron compound microdistribution in an oral cancer model; Taylor & Francis Ltd; International Journal Of Radiation Biology; 91; 4; 4-2015; 329-335
0955-3002
CONICET Digital
CONICET
url http://hdl.handle.net/11336/111429
identifier_str_mv Portu, Agustina Mariana; Molinari, Ana Julia; Thorp, Silvia Inés; Pozzi, Emiliano César Cayetano; Curotto, Paula; et al.; Neutron autoradiography to study boron compound microdistribution in an oral cancer model; Taylor & Francis Ltd; International Journal Of Radiation Biology; 91; 4; 4-2015; 329-335
0955-3002
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3109/09553002.2014.995381
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.3109/09553002.2014.995381
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis Ltd
publisher.none.fl_str_mv Taylor & Francis Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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