Optical density of high fluence autoradiographic images to study boron microdistribution: validation of the technique in tissue sections

Autores
Espain, Maria Sol; Viglietti, J. S.; Vidal, C.; Martin, D.; Trivillin, Verónica Andrea; Monti Hughes, Andrea; Palmieri, M. A.; De Leo, L.; Garabalino, M. A.; Curotto, P.; Thorp, S. I.; Pozzi, E. C. C.; Saint Martin, G.; Portu, Agustina Mariana
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The distribution of boron in biological samples coming from BNCT protocols can be determined by the analysis of autoradiographic images formed by nuclear tracks on polymeric detectors. The tissue section is first explored to delimit regions of interest (ROIs), and then it is removed in order to reveal nuclear tracks through an etching process. A quantitative autoradiographic technique was developed to measure the boron concentration in the sample by counting the nuclear tracks on the detector. However, under certain circumstances, rapid measurements of boron distribution are required. For that purpose, a methodology was developed, allowing a rapid and easy determination of boron concentration and distribution in a tissue section by measuring grey levels in the corresponding high fluence autoradiographic image (HFA). The grey levels are then converted to optical density values which are then correlated with boron concentration values. In order to validate the technique, consecutive sections of several tissue samples were analyzed by the conventional counting method and by HFA. The image analysis and boron concentration estimation can be carried out in under 15 min, and relative differences between zones can be easily determined.
Fil: Espain, Maria Sol. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Viglietti, J. S.. Comisión Nacional de Energía Atómica; Argentina
Fil: Vidal, C.. Universidad Favaloro. Facultad de Ingeniería y Ciencias Exactas y Naturales; Argentina
Fil: Martin, D.. Comisión Nacional de Energía Atómica; Argentina
Fil: Trivillin, Verónica Andrea. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Monti Hughes, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Palmieri, M. A.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: De Leo, L.. Universidad Favaloro. Facultad de Ingeniería y Ciencias Exactas y Naturales; Argentina
Fil: Garabalino, M. A.. Comisión Nacional de Energía Atómica; Argentina
Fil: Curotto, P.. Comisión Nacional de Energía Atómica; Argentina
Fil: Thorp, S. I.. Comisión Nacional de Energía Atómica; Argentina
Fil: Pozzi, E. C. C.. Comisión Nacional de Energía Atómica; Argentina
Fil: Saint Martin, G.. Comisión Nacional de Energía Atómica; Argentina
Fil: Portu, Agustina Mariana. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
NEUTRON AUTORADIOGRAPHY
BORON IMAGING
BNCT
OPTICAL DENSITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/231290
Acceder
id CONICETDig_b192e1b7112519b67c3cd48a7a172e50
oai_identifier_str oai:ri.conicet.gov.ar:11336/231290
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Optical density of high fluence autoradiographic images to study boron microdistribution: validation of the technique in tissue sectionsEspain, Maria SolViglietti, J. S.Vidal, C.Martin, D.Trivillin, Verónica AndreaMonti Hughes, AndreaPalmieri, M. A.De Leo, L.Garabalino, M. A.Curotto, P.Thorp, S. I.Pozzi, E. C. C.Saint Martin, G.Portu, Agustina MarianaNEUTRON AUTORADIOGRAPHYBORON IMAGINGBNCTOPTICAL DENSITYhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1The distribution of boron in biological samples coming from BNCT protocols can be determined by the analysis of autoradiographic images formed by nuclear tracks on polymeric detectors. The tissue section is first explored to delimit regions of interest (ROIs), and then it is removed in order to reveal nuclear tracks through an etching process. A quantitative autoradiographic technique was developed to measure the boron concentration in the sample by counting the nuclear tracks on the detector. However, under certain circumstances, rapid measurements of boron distribution are required. For that purpose, a methodology was developed, allowing a rapid and easy determination of boron concentration and distribution in a tissue section by measuring grey levels in the corresponding high fluence autoradiographic image (HFA). The grey levels are then converted to optical density values which are then correlated with boron concentration values. In order to validate the technique, consecutive sections of several tissue samples were analyzed by the conventional counting method and by HFA. The image analysis and boron concentration estimation can be carried out in under 15 min, and relative differences between zones can be easily determined.Fil: Espain, Maria Sol. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Viglietti, J. S.. Comisión Nacional de Energía Atómica; ArgentinaFil: Vidal, C.. Universidad Favaloro. Facultad de Ingeniería y Ciencias Exactas y Naturales; ArgentinaFil: Martin, D.. Comisión Nacional de Energía Atómica; ArgentinaFil: Trivillin, Verónica Andrea. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Monti Hughes, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; ArgentinaFil: Palmieri, M. A.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: De Leo, L.. Universidad Favaloro. Facultad de Ingeniería y Ciencias Exactas y Naturales; ArgentinaFil: Garabalino, M. A.. Comisión Nacional de Energía Atómica; ArgentinaFil: Curotto, P.. Comisión Nacional de Energía Atómica; ArgentinaFil: Thorp, S. I.. Comisión Nacional de Energía Atómica; ArgentinaFil: Pozzi, E. C. C.. Comisión Nacional de Energía Atómica; ArgentinaFil: Saint Martin, G.. Comisión Nacional de Energía Atómica; ArgentinaFil: Portu, Agustina Mariana. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFrontiers Media2024-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/231290Espain, Maria Sol; Viglietti, J. S.; Vidal, C.; Martin, D.; Trivillin, Verónica Andrea; et al.; Optical density of high fluence autoradiographic images to study boron microdistribution: validation of the technique in tissue sections; Frontiers Media; Frontiers in Nuclear Engineering; 3; 3-2024; 1-102813-3412CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fnuen.2024.1363880/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fnuen.2024.1363880info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:57Zoai:ri.conicet.gov.ar:11336/231290instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:57.553CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Optical density of high fluence autoradiographic images to study boron microdistribution: validation of the technique in tissue sections
title Optical density of high fluence autoradiographic images to study boron microdistribution: validation of the technique in tissue sections
spellingShingle Optical density of high fluence autoradiographic images to study boron microdistribution: validation of the technique in tissue sections
Espain, Maria Sol
NEUTRON AUTORADIOGRAPHY
BORON IMAGING
BNCT
OPTICAL DENSITY
title_short Optical density of high fluence autoradiographic images to study boron microdistribution: validation of the technique in tissue sections
title_full Optical density of high fluence autoradiographic images to study boron microdistribution: validation of the technique in tissue sections
title_fullStr Optical density of high fluence autoradiographic images to study boron microdistribution: validation of the technique in tissue sections
title_full_unstemmed Optical density of high fluence autoradiographic images to study boron microdistribution: validation of the technique in tissue sections
title_sort Optical density of high fluence autoradiographic images to study boron microdistribution: validation of the technique in tissue sections
dc.creator.none.fl_str_mv Espain, Maria Sol
Viglietti, J. S.
Vidal, C.
Martin, D.
Trivillin, Verónica Andrea
Monti Hughes, Andrea
Palmieri, M. A.
De Leo, L.
Garabalino, M. A.
Curotto, P.
Thorp, S. I.
Pozzi, E. C. C.
Saint Martin, G.
Portu, Agustina Mariana
author Espain, Maria Sol
author_facet Espain, Maria Sol
Viglietti, J. S.
Vidal, C.
Martin, D.
Trivillin, Verónica Andrea
Monti Hughes, Andrea
Palmieri, M. A.
De Leo, L.
Garabalino, M. A.
Curotto, P.
Thorp, S. I.
Pozzi, E. C. C.
Saint Martin, G.
Portu, Agustina Mariana
author_role author
author2 Viglietti, J. S.
Vidal, C.
Martin, D.
Trivillin, Verónica Andrea
Monti Hughes, Andrea
Palmieri, M. A.
De Leo, L.
Garabalino, M. A.
Curotto, P.
Thorp, S. I.
Pozzi, E. C. C.
Saint Martin, G.
Portu, Agustina Mariana
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv NEUTRON AUTORADIOGRAPHY
BORON IMAGING
BNCT
OPTICAL DENSITY
topic NEUTRON AUTORADIOGRAPHY
BORON IMAGING
BNCT
OPTICAL DENSITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The distribution of boron in biological samples coming from BNCT protocols can be determined by the analysis of autoradiographic images formed by nuclear tracks on polymeric detectors. The tissue section is first explored to delimit regions of interest (ROIs), and then it is removed in order to reveal nuclear tracks through an etching process. A quantitative autoradiographic technique was developed to measure the boron concentration in the sample by counting the nuclear tracks on the detector. However, under certain circumstances, rapid measurements of boron distribution are required. For that purpose, a methodology was developed, allowing a rapid and easy determination of boron concentration and distribution in a tissue section by measuring grey levels in the corresponding high fluence autoradiographic image (HFA). The grey levels are then converted to optical density values which are then correlated with boron concentration values. In order to validate the technique, consecutive sections of several tissue samples were analyzed by the conventional counting method and by HFA. The image analysis and boron concentration estimation can be carried out in under 15 min, and relative differences between zones can be easily determined.
Fil: Espain, Maria Sol. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Viglietti, J. S.. Comisión Nacional de Energía Atómica; Argentina
Fil: Vidal, C.. Universidad Favaloro. Facultad de Ingeniería y Ciencias Exactas y Naturales; Argentina
Fil: Martin, D.. Comisión Nacional de Energía Atómica; Argentina
Fil: Trivillin, Verónica Andrea. Comisión Nacional de Energía Atómica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Monti Hughes, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Comisión Nacional de Energía Atómica; Argentina
Fil: Palmieri, M. A.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: De Leo, L.. Universidad Favaloro. Facultad de Ingeniería y Ciencias Exactas y Naturales; Argentina
Fil: Garabalino, M. A.. Comisión Nacional de Energía Atómica; Argentina
Fil: Curotto, P.. Comisión Nacional de Energía Atómica; Argentina
Fil: Thorp, S. I.. Comisión Nacional de Energía Atómica; Argentina
Fil: Pozzi, E. C. C.. Comisión Nacional de Energía Atómica; Argentina
Fil: Saint Martin, G.. Comisión Nacional de Energía Atómica; Argentina
Fil: Portu, Agustina Mariana. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The distribution of boron in biological samples coming from BNCT protocols can be determined by the analysis of autoradiographic images formed by nuclear tracks on polymeric detectors. The tissue section is first explored to delimit regions of interest (ROIs), and then it is removed in order to reveal nuclear tracks through an etching process. A quantitative autoradiographic technique was developed to measure the boron concentration in the sample by counting the nuclear tracks on the detector. However, under certain circumstances, rapid measurements of boron distribution are required. For that purpose, a methodology was developed, allowing a rapid and easy determination of boron concentration and distribution in a tissue section by measuring grey levels in the corresponding high fluence autoradiographic image (HFA). The grey levels are then converted to optical density values which are then correlated with boron concentration values. In order to validate the technique, consecutive sections of several tissue samples were analyzed by the conventional counting method and by HFA. The image analysis and boron concentration estimation can be carried out in under 15 min, and relative differences between zones can be easily determined.
publishDate 2024
dc.date.none.fl_str_mv 2024-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/231290
Espain, Maria Sol; Viglietti, J. S.; Vidal, C.; Martin, D.; Trivillin, Verónica Andrea; et al.; Optical density of high fluence autoradiographic images to study boron microdistribution: validation of the technique in tissue sections; Frontiers Media; Frontiers in Nuclear Engineering; 3; 3-2024; 1-10
2813-3412
CONICET Digital
CONICET
url http://hdl.handle.net/11336/231290
identifier_str_mv Espain, Maria Sol; Viglietti, J. S.; Vidal, C.; Martin, D.; Trivillin, Verónica Andrea; et al.; Optical density of high fluence autoradiographic images to study boron microdistribution: validation of the technique in tissue sections; Frontiers Media; Frontiers in Nuclear Engineering; 3; 3-2024; 1-10
2813-3412
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fnuen.2024.1363880/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fnuen.2024.1363880
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268827021213696
score 13.13397

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