A silver staining method for single-cell gel assay

Autores
Nadin, Silvina Beatriz; Nadin, Silvina Beatriz; Vargas Roig, Laura Maria; Vargas Roig, Laura Maria; Ciocca, Daniel Ramon; Ciocca, Daniel Ramon
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The single-cell gel assay (comet assay) is a very useful microelectrophoretic technique for evaluation of DNA damage and repair in individual cells. Usually, the comets are visualized and evaluated with fluorescent DNA stains. This staining requires specific equipment (e.g., a high-quality fluorescence microscope), the slides must be analyzed immediately, and they cannot be stored for long periods of time. Here we describe, using human lymphocytes, some modifications of the silver staining for comets that significantly increase the sensitivity/reproducibility of the assay. This silver staining was compared with fluorescence staining and commercial silver stains.
The single-cell gel assay (comet assay) is a very useful microelectrophoretic technique for evaluation of DNA damage and repair in individual cells. Usually, the comets are visualized and evaluated with fluorescent DNA stains. This staining requires specific equipment (e.g., a high-quality fluorescence microscope), the slides must be analyzed immediately, and they cannot be stored for long periods of time. Here we describe, using human lymphocytes, some modifications of the silver staining for comets that significantly increase the sensitivity/reproducibility of the assay. This silver staining was compared with fluorescence staining and commercial silver stains.
Fil: Nadin, Silvina Beatriz. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina
Fil: Nadin, Silvina Beatriz. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina
Fil: Vargas Roig, Laura Maria. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina
Fil: Vargas Roig, Laura Maria. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina
Fil: Ciocca, Daniel Ramon. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina
Fil: Ciocca, Daniel Ramon. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina
Materia
COMET ASSAY
COMET ASSAY
DNA DAMAGE
DNA DAMAGE
SILVER STAINING
SILVER STAINING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/128665
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/128665
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A silver staining method for single-cell gel assayA silver staining method for single-cell gel assayNadin, Silvina BeatrizNadin, Silvina BeatrizVargas Roig, Laura MariaVargas Roig, Laura MariaCiocca, Daniel RamonCiocca, Daniel RamonCOMET ASSAYCOMET ASSAYDNA DAMAGEDNA DAMAGESILVER STAININGSILVER STAININGhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3The single-cell gel assay (comet assay) is a very useful microelectrophoretic technique for evaluation of DNA damage and repair in individual cells. Usually, the comets are visualized and evaluated with fluorescent DNA stains. This staining requires specific equipment (e.g., a high-quality fluorescence microscope), the slides must be analyzed immediately, and they cannot be stored for long periods of time. Here we describe, using human lymphocytes, some modifications of the silver staining for comets that significantly increase the sensitivity/reproducibility of the assay. This silver staining was compared with fluorescence staining and commercial silver stains.The single-cell gel assay (comet assay) is a very useful microelectrophoretic technique for evaluation of DNA damage and repair in individual cells. Usually, the comets are visualized and evaluated with fluorescent DNA stains. This staining requires specific equipment (e.g., a high-quality fluorescence microscope), the slides must be analyzed immediately, and they cannot be stored for long periods of time. Here we describe, using human lymphocytes, some modifications of the silver staining for comets that significantly increase the sensitivity/reproducibility of the assay. This silver staining was compared with fluorescence staining and commercial silver stains.Fil: Nadin, Silvina Beatriz. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); ArgentinaFil: Nadin, Silvina Beatriz. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); ArgentinaFil: Vargas Roig, Laura Maria. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); ArgentinaFil: Vargas Roig, Laura Maria. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); ArgentinaFil: Ciocca, Daniel Ramon. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); ArgentinaFil: Ciocca, Daniel Ramon. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); ArgentinaSAGE PublicationsSAGE Publications2001-122001-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/128665Nadin, Silvina Beatriz; Vargas Roig, Laura Maria; Ciocca, Daniel Ramon; A silver staining method for single-cell gel assay; SAGE Publications; The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society; 49; 9; 12-2001; 1183-1186Nadin, Silvina Beatriz; Vargas Roig, Laura Maria; Ciocca, Daniel Ramon; A silver staining method for single-cell gel assay; SAGE Publications; The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society; 49; 9; 12-2001; 1183-11860022-15540022-1554CONICET DigitalCONICETengenginfo:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/pdf/10.1177/002215540104900912info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/pdf/10.1177/002215540104900912info:eu-repo/semantics/altIdentifier/doi/10.1177/002215540104900912info:eu-repo/semantics/altIdentifier/doi/10.1177/002215540104900912info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:48Zoai:ri.conicet.gov.ar:11336/128665instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:49.247CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A silver staining method for single-cell gel assay
A silver staining method for single-cell gel assay
title A silver staining method for single-cell gel assay
spellingShingle A silver staining method for single-cell gel assay
Nadin, Silvina Beatriz
COMET ASSAY
COMET ASSAY
DNA DAMAGE
DNA DAMAGE
SILVER STAINING
SILVER STAINING
title_short A silver staining method for single-cell gel assay
title_full A silver staining method for single-cell gel assay
title_fullStr A silver staining method for single-cell gel assay
title_full_unstemmed A silver staining method for single-cell gel assay
title_sort A silver staining method for single-cell gel assay
dc.creator.none.fl_str_mv Nadin, Silvina Beatriz
Nadin, Silvina Beatriz
Vargas Roig, Laura Maria
Vargas Roig, Laura Maria
Ciocca, Daniel Ramon
Ciocca, Daniel Ramon
author Nadin, Silvina Beatriz
author_facet Nadin, Silvina Beatriz
Vargas Roig, Laura Maria
Ciocca, Daniel Ramon
author_role author
author2 Vargas Roig, Laura Maria
Ciocca, Daniel Ramon
author2_role author
author
dc.subject.none.fl_str_mv COMET ASSAY
COMET ASSAY
DNA DAMAGE
DNA DAMAGE
SILVER STAINING
SILVER STAINING
topic COMET ASSAY
COMET ASSAY
DNA DAMAGE
DNA DAMAGE
SILVER STAINING
SILVER STAINING
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The single-cell gel assay (comet assay) is a very useful microelectrophoretic technique for evaluation of DNA damage and repair in individual cells. Usually, the comets are visualized and evaluated with fluorescent DNA stains. This staining requires specific equipment (e.g., a high-quality fluorescence microscope), the slides must be analyzed immediately, and they cannot be stored for long periods of time. Here we describe, using human lymphocytes, some modifications of the silver staining for comets that significantly increase the sensitivity/reproducibility of the assay. This silver staining was compared with fluorescence staining and commercial silver stains.
The single-cell gel assay (comet assay) is a very useful microelectrophoretic technique for evaluation of DNA damage and repair in individual cells. Usually, the comets are visualized and evaluated with fluorescent DNA stains. This staining requires specific equipment (e.g., a high-quality fluorescence microscope), the slides must be analyzed immediately, and they cannot be stored for long periods of time. Here we describe, using human lymphocytes, some modifications of the silver staining for comets that significantly increase the sensitivity/reproducibility of the assay. This silver staining was compared with fluorescence staining and commercial silver stains.
Fil: Nadin, Silvina Beatriz. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina
Fil: Nadin, Silvina Beatriz. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina
Fil: Vargas Roig, Laura Maria. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina
Fil: Vargas Roig, Laura Maria. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina
Fil: Ciocca, Daniel Ramon. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina
Fil: Ciocca, Daniel Ramon. Fundación Argentina para la Investigación del Cáncer; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Reproducción y Lactancia (i); Argentina
description The single-cell gel assay (comet assay) is a very useful microelectrophoretic technique for evaluation of DNA damage and repair in individual cells. Usually, the comets are visualized and evaluated with fluorescent DNA stains. This staining requires specific equipment (e.g., a high-quality fluorescence microscope), the slides must be analyzed immediately, and they cannot be stored for long periods of time. Here we describe, using human lymphocytes, some modifications of the silver staining for comets that significantly increase the sensitivity/reproducibility of the assay. This silver staining was compared with fluorescence staining and commercial silver stains.
publishDate 2001
dc.date.none.fl_str_mv 2001-12
2001-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/128665
Nadin, Silvina Beatriz; Vargas Roig, Laura Maria; Ciocca, Daniel Ramon; A silver staining method for single-cell gel assay; SAGE Publications; The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society; 49; 9; 12-2001; 1183-1186
Nadin, Silvina Beatriz; Vargas Roig, Laura Maria; Ciocca, Daniel Ramon; A silver staining method for single-cell gel assay; SAGE Publications; The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society; 49; 9; 12-2001; 1183-1186
0022-1554
0022-1554
CONICET Digital
CONICET
url http://hdl.handle.net/11336/128665
identifier_str_mv Nadin, Silvina Beatriz; Vargas Roig, Laura Maria; Ciocca, Daniel Ramon; A silver staining method for single-cell gel assay; SAGE Publications; The Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society; 49; 9; 12-2001; 1183-1186
0022-1554
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/pdf/10.1177/002215540104900912
info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/pdf/10.1177/002215540104900912
info:eu-repo/semantics/altIdentifier/doi/10.1177/002215540104900912
info:eu-repo/semantics/altIdentifier/doi/10.1177/002215540104900912
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv SAGE Publications
SAGE Publications
publisher.none.fl_str_mv SAGE Publications
SAGE Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269425706729472
score 13.13397

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