Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation
- Autores
- Güerci, Alba Mabel; Zúñiga, L.; Marcos, R.
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Individual radiosensitivity is an individual characteristic associated with an increased reaction to ionizing radiation. The purpose of our work is to establish a dose-response curve useful to classify individuals as radiosensitive or radioresistant. Thus, a dose-response curve was constructed by measuring in vitro responses to increasing doses (0 to 8 Gy) of gamma radiation in the comet assay. The obtained curve fit well with a linear equation in the range of 0 to 8 Gy. The overall dose-response curve was constructed for percent DNA in tail, as a measure of the genetic damage induced by irradiation. To probe the goodness of the constructed curve, a validation study was carried out with whole blood from two donors in a blind study. Results show that, for the two applied doses (2 and 6 Gy), the obtained values fit well inside the interval of confidence of the curve. In conclusion, our results demonstrate the usefulness of the comet assay in determining individual responses to defined doses of gamma radiation. The standard dose-response curve constructed may be used to detect individuals departing from reference values.
Fil: Güerci, Alba Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universitat Autònoma de Barcelona; España
Fil: Zúñiga, L.. Universitat Autònoma de Barcelona; España
Fil: Marcos, R.. Universitat Autònoma de Barcelona; España. Universidad Carlos III de Madrid. Instituto de Salud; España - Materia
-
Radiosensitivity
Ionizing Radiation
Comet Assay - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/78884
Ver los metadatos del registro completo
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Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing RadiationGüerci, Alba MabelZúñiga, L.Marcos, R.RadiosensitivityIonizing RadiationComet Assayhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Individual radiosensitivity is an individual characteristic associated with an increased reaction to ionizing radiation. The purpose of our work is to establish a dose-response curve useful to classify individuals as radiosensitive or radioresistant. Thus, a dose-response curve was constructed by measuring in vitro responses to increasing doses (0 to 8 Gy) of gamma radiation in the comet assay. The obtained curve fit well with a linear equation in the range of 0 to 8 Gy. The overall dose-response curve was constructed for percent DNA in tail, as a measure of the genetic damage induced by irradiation. To probe the goodness of the constructed curve, a validation study was carried out with whole blood from two donors in a blind study. Results show that, for the two applied doses (2 and 6 Gy), the obtained values fit well inside the interval of confidence of the curve. In conclusion, our results demonstrate the usefulness of the comet assay in determining individual responses to defined doses of gamma radiation. The standard dose-response curve constructed may be used to detect individuals departing from reference values.Fil: Güerci, Alba Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universitat Autònoma de Barcelona; EspañaFil: Zúñiga, L.. Universitat Autònoma de Barcelona; EspañaFil: Marcos, R.. Universitat Autònoma de Barcelona; España. Universidad Carlos III de Madrid. Instituto de Salud; EspañaTaylor & Francis2011-08-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/78884Güerci, Alba Mabel; Zúñiga, L.; Marcos, R.; Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation ; Taylor & Francis; Journal of Toxicology and Environmental Health-Part A-Current Issues; 74; 15-16; 27-8-2011; 1087-10931528-7394CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/15287394.2011.582318info:eu-repo/semantics/altIdentifier/doi/10.1080/15287394.2011.582318info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:40:59Zoai:ri.conicet.gov.ar:11336/78884instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:40:59.445CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation |
title |
Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation |
spellingShingle |
Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation Güerci, Alba Mabel Radiosensitivity Ionizing Radiation Comet Assay |
title_short |
Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation |
title_full |
Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation |
title_fullStr |
Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation |
title_full_unstemmed |
Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation |
title_sort |
Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation |
dc.creator.none.fl_str_mv |
Güerci, Alba Mabel Zúñiga, L. Marcos, R. |
author |
Güerci, Alba Mabel |
author_facet |
Güerci, Alba Mabel Zúñiga, L. Marcos, R. |
author_role |
author |
author2 |
Zúñiga, L. Marcos, R. |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Radiosensitivity Ionizing Radiation Comet Assay |
topic |
Radiosensitivity Ionizing Radiation Comet Assay |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Individual radiosensitivity is an individual characteristic associated with an increased reaction to ionizing radiation. The purpose of our work is to establish a dose-response curve useful to classify individuals as radiosensitive or radioresistant. Thus, a dose-response curve was constructed by measuring in vitro responses to increasing doses (0 to 8 Gy) of gamma radiation in the comet assay. The obtained curve fit well with a linear equation in the range of 0 to 8 Gy. The overall dose-response curve was constructed for percent DNA in tail, as a measure of the genetic damage induced by irradiation. To probe the goodness of the constructed curve, a validation study was carried out with whole blood from two donors in a blind study. Results show that, for the two applied doses (2 and 6 Gy), the obtained values fit well inside the interval of confidence of the curve. In conclusion, our results demonstrate the usefulness of the comet assay in determining individual responses to defined doses of gamma radiation. The standard dose-response curve constructed may be used to detect individuals departing from reference values. Fil: Güerci, Alba Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universitat Autònoma de Barcelona; España Fil: Zúñiga, L.. Universitat Autònoma de Barcelona; España Fil: Marcos, R.. Universitat Autònoma de Barcelona; España. Universidad Carlos III de Madrid. Instituto de Salud; España |
description |
Individual radiosensitivity is an individual characteristic associated with an increased reaction to ionizing radiation. The purpose of our work is to establish a dose-response curve useful to classify individuals as radiosensitive or radioresistant. Thus, a dose-response curve was constructed by measuring in vitro responses to increasing doses (0 to 8 Gy) of gamma radiation in the comet assay. The obtained curve fit well with a linear equation in the range of 0 to 8 Gy. The overall dose-response curve was constructed for percent DNA in tail, as a measure of the genetic damage induced by irradiation. To probe the goodness of the constructed curve, a validation study was carried out with whole blood from two donors in a blind study. Results show that, for the two applied doses (2 and 6 Gy), the obtained values fit well inside the interval of confidence of the curve. In conclusion, our results demonstrate the usefulness of the comet assay in determining individual responses to defined doses of gamma radiation. The standard dose-response curve constructed may be used to detect individuals departing from reference values. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-08-27 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/78884 Güerci, Alba Mabel; Zúñiga, L.; Marcos, R.; Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation ; Taylor & Francis; Journal of Toxicology and Environmental Health-Part A-Current Issues; 74; 15-16; 27-8-2011; 1087-1093 1528-7394 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/78884 |
identifier_str_mv |
Güerci, Alba Mabel; Zúñiga, L.; Marcos, R.; Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation ; Taylor & Francis; Journal of Toxicology and Environmental Health-Part A-Current Issues; 74; 15-16; 27-8-2011; 1087-1093 1528-7394 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/15287394.2011.582318 info:eu-repo/semantics/altIdentifier/doi/10.1080/15287394.2011.582318 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis |
publisher.none.fl_str_mv |
Taylor & Francis |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614439326711808 |
score |
13.070432 |