Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation

Autores
Güerci, Alba Mabel; Zúñiga, L.; Marcos, R.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Individual radiosensitivity is an individual characteristic associated with an increased reaction to ionizing radiation. The purpose of our work is to establish a dose-response curve useful to classify individuals as radiosensitive or radioresistant. Thus, a dose-response curve was constructed by measuring in vitro responses to increasing doses (0 to 8 Gy) of gamma radiation in the comet assay. The obtained curve fit well with a linear equation in the range of 0 to 8 Gy. The overall dose-response curve was constructed for percent DNA in tail, as a measure of the genetic damage induced by irradiation. To probe the goodness of the constructed curve, a validation study was carried out with whole blood from two donors in a blind study. Results show that, for the two applied doses (2 and 6 Gy), the obtained values fit well inside the interval of confidence of the curve. In conclusion, our results demonstrate the usefulness of the comet assay in determining individual responses to defined doses of gamma radiation. The standard dose-response curve constructed may be used to detect individuals departing from reference values.
Fil: Güerci, Alba Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universitat Autònoma de Barcelona; España
Fil: Zúñiga, L.. Universitat Autònoma de Barcelona; España
Fil: Marcos, R.. Universitat Autònoma de Barcelona; España. Universidad Carlos III de Madrid. Instituto de Salud; España
Materia
Radiosensitivity
Ionizing Radiation
Comet Assay
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/78884
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing RadiationGüerci, Alba MabelZúñiga, L.Marcos, R.RadiosensitivityIonizing RadiationComet Assayhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Individual radiosensitivity is an individual characteristic associated with an increased reaction to ionizing radiation. The purpose of our work is to establish a dose-response curve useful to classify individuals as radiosensitive or radioresistant. Thus, a dose-response curve was constructed by measuring in vitro responses to increasing doses (0 to 8 Gy) of gamma radiation in the comet assay. The obtained curve fit well with a linear equation in the range of 0 to 8 Gy. The overall dose-response curve was constructed for percent DNA in tail, as a measure of the genetic damage induced by irradiation. To probe the goodness of the constructed curve, a validation study was carried out with whole blood from two donors in a blind study. Results show that, for the two applied doses (2 and 6 Gy), the obtained values fit well inside the interval of confidence of the curve. In conclusion, our results demonstrate the usefulness of the comet assay in determining individual responses to defined doses of gamma radiation. The standard dose-response curve constructed may be used to detect individuals departing from reference values.Fil: Güerci, Alba Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universitat Autònoma de Barcelona; EspañaFil: Zúñiga, L.. Universitat Autònoma de Barcelona; EspañaFil: Marcos, R.. Universitat Autònoma de Barcelona; España. Universidad Carlos III de Madrid. Instituto de Salud; EspañaTaylor & Francis2011-08-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/78884Güerci, Alba Mabel; Zúñiga, L.; Marcos, R.; Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation ; Taylor & Francis; Journal of Toxicology and Environmental Health-Part A-Current Issues; 74; 15-16; 27-8-2011; 1087-10931528-7394CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/15287394.2011.582318info:eu-repo/semantics/altIdentifier/doi/10.1080/15287394.2011.582318info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:38:50Zoai:ri.conicet.gov.ar:11336/78884instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:38:50.783CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation
title Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation
spellingShingle Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation
Güerci, Alba Mabel
Radiosensitivity
Ionizing Radiation
Comet Assay
title_short Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation
title_full Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation
title_fullStr Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation
title_full_unstemmed Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation
title_sort Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation
dc.creator.none.fl_str_mv Güerci, Alba Mabel
Zúñiga, L.
Marcos, R.
author Güerci, Alba Mabel
author_facet Güerci, Alba Mabel
Zúñiga, L.
Marcos, R.
author_role author
author2 Zúñiga, L.
Marcos, R.
author2_role author
author
dc.subject.none.fl_str_mv Radiosensitivity
Ionizing Radiation
Comet Assay
topic Radiosensitivity
Ionizing Radiation
Comet Assay
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Individual radiosensitivity is an individual characteristic associated with an increased reaction to ionizing radiation. The purpose of our work is to establish a dose-response curve useful to classify individuals as radiosensitive or radioresistant. Thus, a dose-response curve was constructed by measuring in vitro responses to increasing doses (0 to 8 Gy) of gamma radiation in the comet assay. The obtained curve fit well with a linear equation in the range of 0 to 8 Gy. The overall dose-response curve was constructed for percent DNA in tail, as a measure of the genetic damage induced by irradiation. To probe the goodness of the constructed curve, a validation study was carried out with whole blood from two donors in a blind study. Results show that, for the two applied doses (2 and 6 Gy), the obtained values fit well inside the interval of confidence of the curve. In conclusion, our results demonstrate the usefulness of the comet assay in determining individual responses to defined doses of gamma radiation. The standard dose-response curve constructed may be used to detect individuals departing from reference values.
Fil: Güerci, Alba Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universitat Autònoma de Barcelona; España
Fil: Zúñiga, L.. Universitat Autònoma de Barcelona; España
Fil: Marcos, R.. Universitat Autònoma de Barcelona; España. Universidad Carlos III de Madrid. Instituto de Salud; España
description Individual radiosensitivity is an individual characteristic associated with an increased reaction to ionizing radiation. The purpose of our work is to establish a dose-response curve useful to classify individuals as radiosensitive or radioresistant. Thus, a dose-response curve was constructed by measuring in vitro responses to increasing doses (0 to 8 Gy) of gamma radiation in the comet assay. The obtained curve fit well with a linear equation in the range of 0 to 8 Gy. The overall dose-response curve was constructed for percent DNA in tail, as a measure of the genetic damage induced by irradiation. To probe the goodness of the constructed curve, a validation study was carried out with whole blood from two donors in a blind study. Results show that, for the two applied doses (2 and 6 Gy), the obtained values fit well inside the interval of confidence of the curve. In conclusion, our results demonstrate the usefulness of the comet assay in determining individual responses to defined doses of gamma radiation. The standard dose-response curve constructed may be used to detect individuals departing from reference values.
publishDate 2011
dc.date.none.fl_str_mv 2011-08-27
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/78884
Güerci, Alba Mabel; Zúñiga, L.; Marcos, R.; Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation ; Taylor & Francis; Journal of Toxicology and Environmental Health-Part A-Current Issues; 74; 15-16; 27-8-2011; 1087-1093
1528-7394
CONICET Digital
CONICET
url http://hdl.handle.net/11336/78884
identifier_str_mv Güerci, Alba Mabel; Zúñiga, L.; Marcos, R.; Construction and Validation of a Dose-Response Curve Using the Comet Assay to Determine Human Radiosensitivity to Ionizing Radiation ; Taylor & Francis; Journal of Toxicology and Environmental Health-Part A-Current Issues; 74; 15-16; 27-8-2011; 1087-1093
1528-7394
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/15287394.2011.582318
info:eu-repo/semantics/altIdentifier/doi/10.1080/15287394.2011.582318
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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