Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity

Autores
Ceballos Mancini, María Paula; Capitaine Funes, Juan; Massa, Estefanía María Alejandra; Cipulli, Germán; Benitez Gil, Alfonso; Capitaine Funes, Carlos; Tozzini, Roberto Italo; Ghersevich, Sergio Albino
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Radiotherapy and chemotherapy cause genotoxic side effects that are highly variable among patients. In this study, we evaluated DNA integrity using the comet assay in peripheral blood lymphocytes from breast cancer patients before ("pre-treatment patients"; n = 47) and after ("post-treatment patients"; n = 24) radiotherapy and/or chemotherapy treatment and from healthy donors (n = 15). Comet evaluation was made by visual (types 0−4) and digital (percentage of DNA remaining in the comet head = % head DNA) analysis. The association between the level of DNA damage and cancer prognostic factors was assessed. The treatments caused a significant increase in DNA damage registered by both visual (p < 0.001) and digital (p < 0.001) analyses. No significant associations between the level of DNA damage in pre-treatment patients and cancer prognostic factors were found. A significant correlation between the comet results from each patient before and after treatment (r = 0.64, p = 0.001) was observed. The % head DNA in post-treatment samples from patients with a high level of DNA damage before treatment (30.3 ± 3.1%, p < 0.01) was lower than in post-treatment samples from patients with a low-to-medium level of DNA damage before therapy (49.2 ± 4.4%). These results support the usefulness of the comet assay as a sensitive technique to evaluate basal DNA status and DNA damage caused by cancer treatments. The comet assay could contribute to treatment decisions, especially by taking into account the patient's basal DNA damage before therapy.
Fil: Ceballos Mancini, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); Argentina
Fil: Capitaine Funes, Juan. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente - Rosario. Hospital Provincial del Centenario; Argentina
Fil: Massa, Estefanía María Alejandra. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Bioquimica Clinica. Bioquimica Clinica; Argentina
Fil: Cipulli, Germán. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente - Rosario. Hospital Provincial del Centenario; Argentina
Fil: Benitez Gil, Alfonso. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente. Hospital Provincial; Argentina
Fil: Capitaine Funes, Carlos. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente - Rosario. Hospital Provincial del Centenario; Argentina
Fil: Tozzini, Roberto Italo. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente - Rosario. Hospital Provincial del Centenario; Argentina
Fil: Ghersevich, Sergio Albino. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Bioquimica Clinica. Bioquimica Clinica; Argentina
Materia
Comet Assay
Genotoxic Damage
Peripheral Blood Lymphocytes
Prognostic Factors
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6097
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/6097
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrityCeballos Mancini, María PaulaCapitaine Funes, JuanMassa, Estefanía María AlejandraCipulli, GermánBenitez Gil, AlfonsoCapitaine Funes, CarlosTozzini, Roberto ItaloGhersevich, Sergio AlbinoComet AssayGenotoxic DamagePeripheral Blood LymphocytesPrognostic Factorshttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Radiotherapy and chemotherapy cause genotoxic side effects that are highly variable among patients. In this study, we evaluated DNA integrity using the comet assay in peripheral blood lymphocytes from breast cancer patients before ("pre-treatment patients"; n = 47) and after ("post-treatment patients"; n = 24) radiotherapy and/or chemotherapy treatment and from healthy donors (n = 15). Comet evaluation was made by visual (types 0−4) and digital (percentage of DNA remaining in the comet head = % head DNA) analysis. The association between the level of DNA damage and cancer prognostic factors was assessed. The treatments caused a significant increase in DNA damage registered by both visual (p < 0.001) and digital (p < 0.001) analyses. No significant associations between the level of DNA damage in pre-treatment patients and cancer prognostic factors were found. A significant correlation between the comet results from each patient before and after treatment (r = 0.64, p = 0.001) was observed. The % head DNA in post-treatment samples from patients with a high level of DNA damage before treatment (30.3 ± 3.1%, p < 0.01) was lower than in post-treatment samples from patients with a low-to-medium level of DNA damage before therapy (49.2 ± 4.4%). These results support the usefulness of the comet assay as a sensitive technique to evaluate basal DNA status and DNA damage caused by cancer treatments. The comet assay could contribute to treatment decisions, especially by taking into account the patient's basal DNA damage before therapy.Fil: Ceballos Mancini, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); ArgentinaFil: Capitaine Funes, Juan. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente - Rosario. Hospital Provincial del Centenario; ArgentinaFil: Massa, Estefanía María Alejandra. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Bioquimica Clinica. Bioquimica Clinica; ArgentinaFil: Cipulli, Germán. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente - Rosario. Hospital Provincial del Centenario; ArgentinaFil: Benitez Gil, Alfonso. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente. Hospital Provincial; ArgentinaFil: Capitaine Funes, Carlos. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente - Rosario. Hospital Provincial del Centenario; ArgentinaFil: Tozzini, Roberto Italo. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente - Rosario. Hospital Provincial del Centenario; ArgentinaFil: Ghersevich, Sergio Albino. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Bioquimica Clinica. Bioquimica Clinica; ArgentinaBegell House Inc2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6097Ceballos Mancini, María Paula; Capitaine Funes, Juan; Massa, Estefanía María Alejandra; Cipulli, Germán; Benitez Gil, Alfonso; et al.; Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity; Begell House Inc; Journal of Environmental Pathology, Toxicology and Oncology; 33; 2; 5-2014; 111-1210731-8898enginfo:eu-repo/semantics/altIdentifier/url/http://www.dl.begellhouse.com/journals/0ff459a57a4c08d0,249005dc454eb8de,570be1034750e71e.htmlinfo:eu-repo/semantics/altIdentifier/doi/10.1615/JEnvironPatholToxicolOncol.2014010592info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:09Zoai:ri.conicet.gov.ar:11336/6097instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:09.851CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity
title Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity
spellingShingle Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity
Ceballos Mancini, María Paula
Comet Assay
Genotoxic Damage
Peripheral Blood Lymphocytes
Prognostic Factors
title_short Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity
title_full Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity
title_fullStr Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity
title_full_unstemmed Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity
title_sort Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity
dc.creator.none.fl_str_mv Ceballos Mancini, María Paula
Capitaine Funes, Juan
Massa, Estefanía María Alejandra
Cipulli, Germán
Benitez Gil, Alfonso
Capitaine Funes, Carlos
Tozzini, Roberto Italo
Ghersevich, Sergio Albino
author Ceballos Mancini, María Paula
author_facet Ceballos Mancini, María Paula
Capitaine Funes, Juan
Massa, Estefanía María Alejandra
Cipulli, Germán
Benitez Gil, Alfonso
Capitaine Funes, Carlos
Tozzini, Roberto Italo
Ghersevich, Sergio Albino
author_role author
author2 Capitaine Funes, Juan
Massa, Estefanía María Alejandra
Cipulli, Germán
Benitez Gil, Alfonso
Capitaine Funes, Carlos
Tozzini, Roberto Italo
Ghersevich, Sergio Albino
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Comet Assay
Genotoxic Damage
Peripheral Blood Lymphocytes
Prognostic Factors
topic Comet Assay
Genotoxic Damage
Peripheral Blood Lymphocytes
Prognostic Factors
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Radiotherapy and chemotherapy cause genotoxic side effects that are highly variable among patients. In this study, we evaluated DNA integrity using the comet assay in peripheral blood lymphocytes from breast cancer patients before ("pre-treatment patients"; n = 47) and after ("post-treatment patients"; n = 24) radiotherapy and/or chemotherapy treatment and from healthy donors (n = 15). Comet evaluation was made by visual (types 0−4) and digital (percentage of DNA remaining in the comet head = % head DNA) analysis. The association between the level of DNA damage and cancer prognostic factors was assessed. The treatments caused a significant increase in DNA damage registered by both visual (p < 0.001) and digital (p < 0.001) analyses. No significant associations between the level of DNA damage in pre-treatment patients and cancer prognostic factors were found. A significant correlation between the comet results from each patient before and after treatment (r = 0.64, p = 0.001) was observed. The % head DNA in post-treatment samples from patients with a high level of DNA damage before treatment (30.3 ± 3.1%, p < 0.01) was lower than in post-treatment samples from patients with a low-to-medium level of DNA damage before therapy (49.2 ± 4.4%). These results support the usefulness of the comet assay as a sensitive technique to evaluate basal DNA status and DNA damage caused by cancer treatments. The comet assay could contribute to treatment decisions, especially by taking into account the patient's basal DNA damage before therapy.
Fil: Ceballos Mancini, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); Argentina
Fil: Capitaine Funes, Juan. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente - Rosario. Hospital Provincial del Centenario; Argentina
Fil: Massa, Estefanía María Alejandra. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Bioquimica Clinica. Bioquimica Clinica; Argentina
Fil: Cipulli, Germán. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente - Rosario. Hospital Provincial del Centenario; Argentina
Fil: Benitez Gil, Alfonso. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente. Hospital Provincial; Argentina
Fil: Capitaine Funes, Carlos. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente - Rosario. Hospital Provincial del Centenario; Argentina
Fil: Tozzini, Roberto Italo. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente - Rosario. Hospital Provincial del Centenario; Argentina
Fil: Ghersevich, Sergio Albino. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Bioquimica Clinica. Bioquimica Clinica; Argentina
description Radiotherapy and chemotherapy cause genotoxic side effects that are highly variable among patients. In this study, we evaluated DNA integrity using the comet assay in peripheral blood lymphocytes from breast cancer patients before ("pre-treatment patients"; n = 47) and after ("post-treatment patients"; n = 24) radiotherapy and/or chemotherapy treatment and from healthy donors (n = 15). Comet evaluation was made by visual (types 0−4) and digital (percentage of DNA remaining in the comet head = % head DNA) analysis. The association between the level of DNA damage and cancer prognostic factors was assessed. The treatments caused a significant increase in DNA damage registered by both visual (p < 0.001) and digital (p < 0.001) analyses. No significant associations between the level of DNA damage in pre-treatment patients and cancer prognostic factors were found. A significant correlation between the comet results from each patient before and after treatment (r = 0.64, p = 0.001) was observed. The % head DNA in post-treatment samples from patients with a high level of DNA damage before treatment (30.3 ± 3.1%, p < 0.01) was lower than in post-treatment samples from patients with a low-to-medium level of DNA damage before therapy (49.2 ± 4.4%). These results support the usefulness of the comet assay as a sensitive technique to evaluate basal DNA status and DNA damage caused by cancer treatments. The comet assay could contribute to treatment decisions, especially by taking into account the patient's basal DNA damage before therapy.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6097
Ceballos Mancini, María Paula; Capitaine Funes, Juan; Massa, Estefanía María Alejandra; Cipulli, Germán; Benitez Gil, Alfonso; et al.; Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity; Begell House Inc; Journal of Environmental Pathology, Toxicology and Oncology; 33; 2; 5-2014; 111-121
0731-8898
url http://hdl.handle.net/11336/6097
identifier_str_mv Ceballos Mancini, María Paula; Capitaine Funes, Juan; Massa, Estefanía María Alejandra; Cipulli, Germán; Benitez Gil, Alfonso; et al.; Relationship between genotoxic effects of breast cancer treatments and patient basal DNA integrity; Begell House Inc; Journal of Environmental Pathology, Toxicology and Oncology; 33; 2; 5-2014; 111-121
0731-8898
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.dl.begellhouse.com/journals/0ff459a57a4c08d0,249005dc454eb8de,570be1034750e71e.html
info:eu-repo/semantics/altIdentifier/doi/10.1615/JEnvironPatholToxicolOncol.2014010592
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Begell House Inc
publisher.none.fl_str_mv Begell House Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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