Para-chlorophenyl alanine induces aggressive behavior by serotonin depletion in male rats and increases tryptophan hydroxylase two and gabaa α1 mrna expression in the olfactory bul...

Autores
Mulle Bernedo, María Belén; Campo Verde Arbocco, Fiorella; Bregonzio Diaz, Claudia; Cabrera Kreiker, Ricardo Jorge
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Decreased serotonin (5-HT) has long been linked to increased aggressive behavior. Tryptophan Hydroxylase (TPH) is an enzyme involved in the 5-HT synthesis, and para- chlorophenyl alanine (pCPA) inhibits its activity. TPH2 mRNA expression presence has been mainly described in the raphe complex rodent’s brain. 5-HT-producing neurons in the raphe project their axons to the olfactory bulb, considered a relevant structure in rodents for establishing social interactions, including aggressive behavior. However, the relationship between the olfactory bulb and aggression in a pCPA 5-HT depletion model has not been studied.Moreover, receptor subunit GABAα1 has been found in the olfactory bulb, and 5-HT depletion could affect GABAA receptor expression in different brain areas. Thus, we aimed to evaluate aggressive behavior, serotonergic activity, and the TPH2 and GABAAα1 mRNAexpression, in the olfactory bulb, after a single pCPA (300mg/kg) or vehicle i.p. administration in male rats. Aggression was tested using a resident intruder test. The olfactory bulb was obtained, and neurochemical and molecular techniques were used to measure 5-HT, 5-HIAA, TPH2, and GABAAα1 mRNA expression, respectively. pCPA administration increased aggressive behavior parameters without affecting locomotion, non-social or social interaction. 5-HT levels were decreased after pCPA administration and its turnover rate, although there were no significant changes in 5-HIAA. TPH2 mRNA expression was increased. GABAAα1 mRNA expression wasincreased in the olfactory bulb. Our results apport evidence to the serotonergic deficiency hypothesis of aggression and highlight the olfactory bulb’s Role as an essential structure for understanding aggressive behavior neurobiological complexity.
Fil: Mulle Bernedo, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Universidad de Mendoza. Facultad de Ciencias de la Salud; Argentina
Fil: Campo Verde Arbocco, Fiorella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza. Facultad de Ciencias de la Salud; Argentina
Fil: Bregonzio Diaz, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza. Facultad de Ciencias de la Salud; Argentina
Materia
RAT RAPHE NUCLEI
OLFATORY BULB
SOCIAL INTERACTION
RESIDENT INTRUDER TEST
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/233312
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/233312
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network_name_str CONICET Digital (CONICET)
spelling Para-chlorophenyl alanine induces aggressive behavior by serotonin depletion in male rats and increases tryptophan hydroxylase two and gabaa α1 mrna expression in the olfactory bulbMulle Bernedo, María BelénCampo Verde Arbocco, FiorellaBregonzio Diaz, ClaudiaCabrera Kreiker, Ricardo JorgeRAT RAPHE NUCLEIOLFATORY BULBSOCIAL INTERACTIONRESIDENT INTRUDER TESThttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Decreased serotonin (5-HT) has long been linked to increased aggressive behavior. Tryptophan Hydroxylase (TPH) is an enzyme involved in the 5-HT synthesis, and para- chlorophenyl alanine (pCPA) inhibits its activity. TPH2 mRNA expression presence has been mainly described in the raphe complex rodent’s brain. 5-HT-producing neurons in the raphe project their axons to the olfactory bulb, considered a relevant structure in rodents for establishing social interactions, including aggressive behavior. However, the relationship between the olfactory bulb and aggression in a pCPA 5-HT depletion model has not been studied.Moreover, receptor subunit GABAα1 has been found in the olfactory bulb, and 5-HT depletion could affect GABAA receptor expression in different brain areas. Thus, we aimed to evaluate aggressive behavior, serotonergic activity, and the TPH2 and GABAAα1 mRNAexpression, in the olfactory bulb, after a single pCPA (300mg/kg) or vehicle i.p. administration in male rats. Aggression was tested using a resident intruder test. The olfactory bulb was obtained, and neurochemical and molecular techniques were used to measure 5-HT, 5-HIAA, TPH2, and GABAAα1 mRNA expression, respectively. pCPA administration increased aggressive behavior parameters without affecting locomotion, non-social or social interaction. 5-HT levels were decreased after pCPA administration and its turnover rate, although there were no significant changes in 5-HIAA. TPH2 mRNA expression was increased. GABAAα1 mRNA expression wasincreased in the olfactory bulb. Our results apport evidence to the serotonergic deficiency hypothesis of aggression and highlight the olfactory bulb’s Role as an essential structure for understanding aggressive behavior neurobiological complexity.Fil: Mulle Bernedo, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Universidad de Mendoza. Facultad de Ciencias de la Salud; ArgentinaFil: Campo Verde Arbocco, Fiorella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza. Facultad de Ciencias de la Salud; ArgentinaFil: Bregonzio Diaz, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza. Facultad de Ciencias de la Salud; ArgentinaBiomedGrid2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/233312Mulle Bernedo, María Belén; Campo Verde Arbocco, Fiorella; Bregonzio Diaz, Claudia; Cabrera Kreiker, Ricardo Jorge; Para-chlorophenyl alanine induces aggressive behavior by serotonin depletion in male rats and increases tryptophan hydroxylase two and gabaa α1 mrna expression in the olfactory bulb; BiomedGrid; American Journal of Biomedical Science & Research; 18; 6; 5-2023; 556-5642642-1747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biomedgrid.com/fulltext/volume18/para-chlorophenyl-alanine-induces-aggressive-behavior-by-serotonin-depletion-in-male-rats-and-increases-tryptophan-hydroxylase-two-and-gabaa-%CE%B11-mrna-expression-in-the-olfactory-bulb.002524.phpinfo:eu-repo/semantics/altIdentifier/doi/10.34297/AJBSR.2023.18.002524info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:38Zoai:ri.conicet.gov.ar:11336/233312instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:38.908CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Para-chlorophenyl alanine induces aggressive behavior by serotonin depletion in male rats and increases tryptophan hydroxylase two and gabaa α1 mrna expression in the olfactory bulb
title Para-chlorophenyl alanine induces aggressive behavior by serotonin depletion in male rats and increases tryptophan hydroxylase two and gabaa α1 mrna expression in the olfactory bulb
spellingShingle Para-chlorophenyl alanine induces aggressive behavior by serotonin depletion in male rats and increases tryptophan hydroxylase two and gabaa α1 mrna expression in the olfactory bulb
Mulle Bernedo, María Belén
RAT RAPHE NUCLEI
OLFATORY BULB
SOCIAL INTERACTION
RESIDENT INTRUDER TEST
title_short Para-chlorophenyl alanine induces aggressive behavior by serotonin depletion in male rats and increases tryptophan hydroxylase two and gabaa α1 mrna expression in the olfactory bulb
title_full Para-chlorophenyl alanine induces aggressive behavior by serotonin depletion in male rats and increases tryptophan hydroxylase two and gabaa α1 mrna expression in the olfactory bulb
title_fullStr Para-chlorophenyl alanine induces aggressive behavior by serotonin depletion in male rats and increases tryptophan hydroxylase two and gabaa α1 mrna expression in the olfactory bulb
title_full_unstemmed Para-chlorophenyl alanine induces aggressive behavior by serotonin depletion in male rats and increases tryptophan hydroxylase two and gabaa α1 mrna expression in the olfactory bulb
title_sort Para-chlorophenyl alanine induces aggressive behavior by serotonin depletion in male rats and increases tryptophan hydroxylase two and gabaa α1 mrna expression in the olfactory bulb
dc.creator.none.fl_str_mv Mulle Bernedo, María Belén
Campo Verde Arbocco, Fiorella
Bregonzio Diaz, Claudia
Cabrera Kreiker, Ricardo Jorge
author Mulle Bernedo, María Belén
author_facet Mulle Bernedo, María Belén
Campo Verde Arbocco, Fiorella
Bregonzio Diaz, Claudia
Cabrera Kreiker, Ricardo Jorge
author_role author
author2 Campo Verde Arbocco, Fiorella
Bregonzio Diaz, Claudia
Cabrera Kreiker, Ricardo Jorge
author2_role author
author
author
dc.subject.none.fl_str_mv RAT RAPHE NUCLEI
OLFATORY BULB
SOCIAL INTERACTION
RESIDENT INTRUDER TEST
topic RAT RAPHE NUCLEI
OLFATORY BULB
SOCIAL INTERACTION
RESIDENT INTRUDER TEST
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Decreased serotonin (5-HT) has long been linked to increased aggressive behavior. Tryptophan Hydroxylase (TPH) is an enzyme involved in the 5-HT synthesis, and para- chlorophenyl alanine (pCPA) inhibits its activity. TPH2 mRNA expression presence has been mainly described in the raphe complex rodent’s brain. 5-HT-producing neurons in the raphe project their axons to the olfactory bulb, considered a relevant structure in rodents for establishing social interactions, including aggressive behavior. However, the relationship between the olfactory bulb and aggression in a pCPA 5-HT depletion model has not been studied.Moreover, receptor subunit GABAα1 has been found in the olfactory bulb, and 5-HT depletion could affect GABAA receptor expression in different brain areas. Thus, we aimed to evaluate aggressive behavior, serotonergic activity, and the TPH2 and GABAAα1 mRNAexpression, in the olfactory bulb, after a single pCPA (300mg/kg) or vehicle i.p. administration in male rats. Aggression was tested using a resident intruder test. The olfactory bulb was obtained, and neurochemical and molecular techniques were used to measure 5-HT, 5-HIAA, TPH2, and GABAAα1 mRNA expression, respectively. pCPA administration increased aggressive behavior parameters without affecting locomotion, non-social or social interaction. 5-HT levels were decreased after pCPA administration and its turnover rate, although there were no significant changes in 5-HIAA. TPH2 mRNA expression was increased. GABAAα1 mRNA expression wasincreased in the olfactory bulb. Our results apport evidence to the serotonergic deficiency hypothesis of aggression and highlight the olfactory bulb’s Role as an essential structure for understanding aggressive behavior neurobiological complexity.
Fil: Mulle Bernedo, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Universidad de Mendoza. Facultad de Ciencias de la Salud; Argentina
Fil: Campo Verde Arbocco, Fiorella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza. Facultad de Ciencias de la Salud; Argentina
Fil: Bregonzio Diaz, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad de Mendoza. Facultad de Ciencias de la Salud; Argentina
description Decreased serotonin (5-HT) has long been linked to increased aggressive behavior. Tryptophan Hydroxylase (TPH) is an enzyme involved in the 5-HT synthesis, and para- chlorophenyl alanine (pCPA) inhibits its activity. TPH2 mRNA expression presence has been mainly described in the raphe complex rodent’s brain. 5-HT-producing neurons in the raphe project their axons to the olfactory bulb, considered a relevant structure in rodents for establishing social interactions, including aggressive behavior. However, the relationship between the olfactory bulb and aggression in a pCPA 5-HT depletion model has not been studied.Moreover, receptor subunit GABAα1 has been found in the olfactory bulb, and 5-HT depletion could affect GABAA receptor expression in different brain areas. Thus, we aimed to evaluate aggressive behavior, serotonergic activity, and the TPH2 and GABAAα1 mRNAexpression, in the olfactory bulb, after a single pCPA (300mg/kg) or vehicle i.p. administration in male rats. Aggression was tested using a resident intruder test. The olfactory bulb was obtained, and neurochemical and molecular techniques were used to measure 5-HT, 5-HIAA, TPH2, and GABAAα1 mRNA expression, respectively. pCPA administration increased aggressive behavior parameters without affecting locomotion, non-social or social interaction. 5-HT levels were decreased after pCPA administration and its turnover rate, although there were no significant changes in 5-HIAA. TPH2 mRNA expression was increased. GABAAα1 mRNA expression wasincreased in the olfactory bulb. Our results apport evidence to the serotonergic deficiency hypothesis of aggression and highlight the olfactory bulb’s Role as an essential structure for understanding aggressive behavior neurobiological complexity.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/233312
Mulle Bernedo, María Belén; Campo Verde Arbocco, Fiorella; Bregonzio Diaz, Claudia; Cabrera Kreiker, Ricardo Jorge; Para-chlorophenyl alanine induces aggressive behavior by serotonin depletion in male rats and increases tryptophan hydroxylase two and gabaa α1 mrna expression in the olfactory bulb; BiomedGrid; American Journal of Biomedical Science & Research; 18; 6; 5-2023; 556-564
2642-1747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/233312
identifier_str_mv Mulle Bernedo, María Belén; Campo Verde Arbocco, Fiorella; Bregonzio Diaz, Claudia; Cabrera Kreiker, Ricardo Jorge; Para-chlorophenyl alanine induces aggressive behavior by serotonin depletion in male rats and increases tryptophan hydroxylase two and gabaa α1 mrna expression in the olfactory bulb; BiomedGrid; American Journal of Biomedical Science & Research; 18; 6; 5-2023; 556-564
2642-1747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://biomedgrid.com/fulltext/volume18/para-chlorophenyl-alanine-induces-aggressive-behavior-by-serotonin-depletion-in-male-rats-and-increases-tryptophan-hydroxylase-two-and-gabaa-%CE%B11-mrna-expression-in-the-olfactory-bulb.002524.php
info:eu-repo/semantics/altIdentifier/doi/10.34297/AJBSR.2023.18.002524
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BiomedGrid
publisher.none.fl_str_mv BiomedGrid
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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