Endosperm and endocarp effects on the Ilex paraguariensis St. Hil. (Aquifoliaceae) seed germination

Autores
Dolce, Natalia Raquel; Mroginski, Luis Amado; Rey de Badaró, Hebe Yolanda
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To develop a procedure for regenerating plants directly from pyrenes, as an alternative to in vitro embryo culture, germination studies were done on maté tree, Ilex paraguariensis, seeds using aseptic conditions. The effect of the endosperm, the woody endocarp and cold treatment on embryo development and germination were examined by culturing isolated embryos, intact, acid-scarified and bisected pyrenes from mature fruits. The results show that maté tree plantlets may be obtained by in vitro culture of bisected pyrenes on solidified (0.65% agar) quarter-strength Murashige and Skoog medium containing 3% sucrose. It is recommended to pre-culture the pyrenes at 4 ± 2°C to obtain a higher germination rate and more vigorous seedlings. This is the first report of the in vitro culture of Ilex pyrenes. Compared to the culture of isolated embryos this procedure shortens the duration of the embryo culture technique and minimizes damage to the young embryos.
Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Materia
Dormancy
Embryo
In Vitro Culture
Maté Tree
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7226
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Endosperm and endocarp effects on the Ilex paraguariensis St. Hil. (Aquifoliaceae) seed germinationDolce, Natalia RaquelMroginski, Luis AmadoRey de Badaró, Hebe YolandaDormancyEmbryoIn Vitro CultureMaté Treehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1To develop a procedure for regenerating plants directly from pyrenes, as an alternative to in vitro embryo culture, germination studies were done on maté tree, Ilex paraguariensis, seeds using aseptic conditions. The effect of the endosperm, the woody endocarp and cold treatment on embryo development and germination were examined by culturing isolated embryos, intact, acid-scarified and bisected pyrenes from mature fruits. The results show that maté tree plantlets may be obtained by in vitro culture of bisected pyrenes on solidified (0.65% agar) quarter-strength Murashige and Skoog medium containing 3% sucrose. It is recommended to pre-culture the pyrenes at 4 ± 2°C to obtain a higher germination rate and more vigorous seedlings. This is the first report of the in vitro culture of Ilex pyrenes. Compared to the culture of isolated embryos this procedure shortens the duration of the embryo culture technique and minimizes damage to the young embryos.Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaFil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaInternational Seed Testing Association2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7226Dolce, Natalia Raquel; Mroginski, Luis Amado; Rey de Badaró, Hebe Yolanda; Endosperm and endocarp effects on the Ilex paraguariensis St. Hil. (Aquifoliaceae) seed germination; International Seed Testing Association; Seed Science And Technology; 38; 2; 4-2010; 441-4480251-09521819-5717enginfo:eu-repo/semantics/altIdentifier/doi/10.15258/sst.2010.38.2.17info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/contentone/ista/sst/2010/00000038/00000002/art00017info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:09Zoai:ri.conicet.gov.ar:11336/7226instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:09.607CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Endosperm and endocarp effects on the Ilex paraguariensis St. Hil. (Aquifoliaceae) seed germination
title Endosperm and endocarp effects on the Ilex paraguariensis St. Hil. (Aquifoliaceae) seed germination
spellingShingle Endosperm and endocarp effects on the Ilex paraguariensis St. Hil. (Aquifoliaceae) seed germination
Dolce, Natalia Raquel
Dormancy
Embryo
In Vitro Culture
Maté Tree
title_short Endosperm and endocarp effects on the Ilex paraguariensis St. Hil. (Aquifoliaceae) seed germination
title_full Endosperm and endocarp effects on the Ilex paraguariensis St. Hil. (Aquifoliaceae) seed germination
title_fullStr Endosperm and endocarp effects on the Ilex paraguariensis St. Hil. (Aquifoliaceae) seed germination
title_full_unstemmed Endosperm and endocarp effects on the Ilex paraguariensis St. Hil. (Aquifoliaceae) seed germination
title_sort Endosperm and endocarp effects on the Ilex paraguariensis St. Hil. (Aquifoliaceae) seed germination
dc.creator.none.fl_str_mv Dolce, Natalia Raquel
Mroginski, Luis Amado
Rey de Badaró, Hebe Yolanda
author Dolce, Natalia Raquel
author_facet Dolce, Natalia Raquel
Mroginski, Luis Amado
Rey de Badaró, Hebe Yolanda
author_role author
author2 Mroginski, Luis Amado
Rey de Badaró, Hebe Yolanda
author2_role author
author
dc.subject.none.fl_str_mv Dormancy
Embryo
In Vitro Culture
Maté Tree
topic Dormancy
Embryo
In Vitro Culture
Maté Tree
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv To develop a procedure for regenerating plants directly from pyrenes, as an alternative to in vitro embryo culture, germination studies were done on maté tree, Ilex paraguariensis, seeds using aseptic conditions. The effect of the endosperm, the woody endocarp and cold treatment on embryo development and germination were examined by culturing isolated embryos, intact, acid-scarified and bisected pyrenes from mature fruits. The results show that maté tree plantlets may be obtained by in vitro culture of bisected pyrenes on solidified (0.65% agar) quarter-strength Murashige and Skoog medium containing 3% sucrose. It is recommended to pre-culture the pyrenes at 4 ± 2°C to obtain a higher germination rate and more vigorous seedlings. This is the first report of the in vitro culture of Ilex pyrenes. Compared to the culture of isolated embryos this procedure shortens the duration of the embryo culture technique and minimizes damage to the young embryos.
Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
description To develop a procedure for regenerating plants directly from pyrenes, as an alternative to in vitro embryo culture, germination studies were done on maté tree, Ilex paraguariensis, seeds using aseptic conditions. The effect of the endosperm, the woody endocarp and cold treatment on embryo development and germination were examined by culturing isolated embryos, intact, acid-scarified and bisected pyrenes from mature fruits. The results show that maté tree plantlets may be obtained by in vitro culture of bisected pyrenes on solidified (0.65% agar) quarter-strength Murashige and Skoog medium containing 3% sucrose. It is recommended to pre-culture the pyrenes at 4 ± 2°C to obtain a higher germination rate and more vigorous seedlings. This is the first report of the in vitro culture of Ilex pyrenes. Compared to the culture of isolated embryos this procedure shortens the duration of the embryo culture technique and minimizes damage to the young embryos.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7226
Dolce, Natalia Raquel; Mroginski, Luis Amado; Rey de Badaró, Hebe Yolanda; Endosperm and endocarp effects on the Ilex paraguariensis St. Hil. (Aquifoliaceae) seed germination; International Seed Testing Association; Seed Science And Technology; 38; 2; 4-2010; 441-448
0251-0952
1819-5717
url http://hdl.handle.net/11336/7226
identifier_str_mv Dolce, Natalia Raquel; Mroginski, Luis Amado; Rey de Badaró, Hebe Yolanda; Endosperm and endocarp effects on the Ilex paraguariensis St. Hil. (Aquifoliaceae) seed germination; International Seed Testing Association; Seed Science And Technology; 38; 2; 4-2010; 441-448
0251-0952
1819-5717
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.15258/sst.2010.38.2.17
info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/contentone/ista/sst/2010/00000038/00000002/art00017
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Seed Testing Association
publisher.none.fl_str_mv International Seed Testing Association
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269991321206784
score 13.13397

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