Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess

Autores
Nair, Rashmi R.; Silveira, Célia M.; Diniz, Mário S.; Almeida, Maria G.; Moura, Jose J. G.; Rivas, Maria Gabriela
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The activity of sulfate-reducing bacteria (SRB) intensifies the problems associated to corrosion of metals and the solution entails significant economic costs. Although molybdate can be used to control the negative effects of these organisms, the mechanisms triggered in the cells exposed to Mo-excess are poorly understood. In this work, the effects of molybdate ions on the growth and morphology of the SRB Desulfovibrio alaskensis G20 (DaG20) were investigated. In addition, the cellular localization, ion uptake and regulation of protein expression were studied. We found that molybdate concentrations ranging between 50 and 150 μM produce a twofold increase in the doubling time with this effect being more significant at 200 μM molybdate (five times increase in the doubling time). It was also observed that 500 μM molybdate completely inhibits the cellular growth. On the context of protein regulation, we found that several enzymes involved in energy metabolism, cellular division and metal uptake processes were particularly influenced under the conditions tested. An overall description of some of the mechanisms involved in the DaG20 adaptation to molybdate-stress conditions is discussed.
Fil: Nair, Rashmi R.. Universidade Nova de Lisboa; Portugal
Fil: Silveira, Célia M.. Universidade Nova de Lisboa; Portugal
Fil: Diniz, Mário S.. Universidade Nova de Lisboa; Portugal
Fil: Almeida, Maria G.. Universidade Nova de Lisboa; Portugal
Fil: Moura, Jose J. G.. Universidade Nova de Lisboa; Portugal
Fil: Rivas, Maria Gabriela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Materia
HYDROGEN CYCLING
MICROBIOLOGICALLY INFLUENCED CORROSION
MOLYBDENUM
SULFATE-REDUCING BACTERIA
TUNGSTEN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84577
Acceder
id CONICETDig_d2f1178cff93b97f36edba046d6ecb78
oai_identifier_str oai:ri.conicet.gov.ar:11336/84577
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excessNair, Rashmi R.Silveira, Célia M.Diniz, Mário S.Almeida, Maria G.Moura, Jose J. G.Rivas, Maria GabrielaHYDROGEN CYCLINGMICROBIOLOGICALLY INFLUENCED CORROSIONMOLYBDENUMSULFATE-REDUCING BACTERIATUNGSTENhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The activity of sulfate-reducing bacteria (SRB) intensifies the problems associated to corrosion of metals and the solution entails significant economic costs. Although molybdate can be used to control the negative effects of these organisms, the mechanisms triggered in the cells exposed to Mo-excess are poorly understood. In this work, the effects of molybdate ions on the growth and morphology of the SRB Desulfovibrio alaskensis G20 (DaG20) were investigated. In addition, the cellular localization, ion uptake and regulation of protein expression were studied. We found that molybdate concentrations ranging between 50 and 150 μM produce a twofold increase in the doubling time with this effect being more significant at 200 μM molybdate (five times increase in the doubling time). It was also observed that 500 μM molybdate completely inhibits the cellular growth. On the context of protein regulation, we found that several enzymes involved in energy metabolism, cellular division and metal uptake processes were particularly influenced under the conditions tested. An overall description of some of the mechanisms involved in the DaG20 adaptation to molybdate-stress conditions is discussed.Fil: Nair, Rashmi R.. Universidade Nova de Lisboa; PortugalFil: Silveira, Célia M.. Universidade Nova de Lisboa; PortugalFil: Diniz, Mário S.. Universidade Nova de Lisboa; PortugalFil: Almeida, Maria G.. Universidade Nova de Lisboa; PortugalFil: Moura, Jose J. G.. Universidade Nova de Lisboa; PortugalFil: Rivas, Maria Gabriela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaSpringer2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84577Nair, Rashmi R.; Silveira, Célia M.; Diniz, Mário S.; Almeida, Maria G.; Moura, Jose J. G.; et al.; Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess; Springer; Journal of Biological Inorganic Chemistry; 20; 2; 3-2015; 311-3220949-8257CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00775-014-1224-4info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00775-014-1224-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:09Zoai:ri.conicet.gov.ar:11336/84577instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:09.893CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess
title Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess
spellingShingle Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess
Nair, Rashmi R.
HYDROGEN CYCLING
MICROBIOLOGICALLY INFLUENCED CORROSION
MOLYBDENUM
SULFATE-REDUCING BACTERIA
TUNGSTEN
title_short Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess
title_full Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess
title_fullStr Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess
title_full_unstemmed Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess
title_sort Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess
dc.creator.none.fl_str_mv Nair, Rashmi R.
Silveira, Célia M.
Diniz, Mário S.
Almeida, Maria G.
Moura, Jose J. G.
Rivas, Maria Gabriela
author Nair, Rashmi R.
author_facet Nair, Rashmi R.
Silveira, Célia M.
Diniz, Mário S.
Almeida, Maria G.
Moura, Jose J. G.
Rivas, Maria Gabriela
author_role author
author2 Silveira, Célia M.
Diniz, Mário S.
Almeida, Maria G.
Moura, Jose J. G.
Rivas, Maria Gabriela
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv HYDROGEN CYCLING
MICROBIOLOGICALLY INFLUENCED CORROSION
MOLYBDENUM
SULFATE-REDUCING BACTERIA
TUNGSTEN
topic HYDROGEN CYCLING
MICROBIOLOGICALLY INFLUENCED CORROSION
MOLYBDENUM
SULFATE-REDUCING BACTERIA
TUNGSTEN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The activity of sulfate-reducing bacteria (SRB) intensifies the problems associated to corrosion of metals and the solution entails significant economic costs. Although molybdate can be used to control the negative effects of these organisms, the mechanisms triggered in the cells exposed to Mo-excess are poorly understood. In this work, the effects of molybdate ions on the growth and morphology of the SRB Desulfovibrio alaskensis G20 (DaG20) were investigated. In addition, the cellular localization, ion uptake and regulation of protein expression were studied. We found that molybdate concentrations ranging between 50 and 150 μM produce a twofold increase in the doubling time with this effect being more significant at 200 μM molybdate (five times increase in the doubling time). It was also observed that 500 μM molybdate completely inhibits the cellular growth. On the context of protein regulation, we found that several enzymes involved in energy metabolism, cellular division and metal uptake processes were particularly influenced under the conditions tested. An overall description of some of the mechanisms involved in the DaG20 adaptation to molybdate-stress conditions is discussed.
Fil: Nair, Rashmi R.. Universidade Nova de Lisboa; Portugal
Fil: Silveira, Célia M.. Universidade Nova de Lisboa; Portugal
Fil: Diniz, Mário S.. Universidade Nova de Lisboa; Portugal
Fil: Almeida, Maria G.. Universidade Nova de Lisboa; Portugal
Fil: Moura, Jose J. G.. Universidade Nova de Lisboa; Portugal
Fil: Rivas, Maria Gabriela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
description The activity of sulfate-reducing bacteria (SRB) intensifies the problems associated to corrosion of metals and the solution entails significant economic costs. Although molybdate can be used to control the negative effects of these organisms, the mechanisms triggered in the cells exposed to Mo-excess are poorly understood. In this work, the effects of molybdate ions on the growth and morphology of the SRB Desulfovibrio alaskensis G20 (DaG20) were investigated. In addition, the cellular localization, ion uptake and regulation of protein expression were studied. We found that molybdate concentrations ranging between 50 and 150 μM produce a twofold increase in the doubling time with this effect being more significant at 200 μM molybdate (five times increase in the doubling time). It was also observed that 500 μM molybdate completely inhibits the cellular growth. On the context of protein regulation, we found that several enzymes involved in energy metabolism, cellular division and metal uptake processes were particularly influenced under the conditions tested. An overall description of some of the mechanisms involved in the DaG20 adaptation to molybdate-stress conditions is discussed.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84577
Nair, Rashmi R.; Silveira, Célia M.; Diniz, Mário S.; Almeida, Maria G.; Moura, Jose J. G.; et al.; Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess; Springer; Journal of Biological Inorganic Chemistry; 20; 2; 3-2015; 311-322
0949-8257
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84577
identifier_str_mv Nair, Rashmi R.; Silveira, Célia M.; Diniz, Mário S.; Almeida, Maria G.; Moura, Jose J. G.; et al.; Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess; Springer; Journal of Biological Inorganic Chemistry; 20; 2; 3-2015; 311-322
0949-8257
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00775-014-1224-4
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00775-014-1224-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270109001842688
score 13.13397

Publicaciones similares