Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491
- Autores
- Mota, Cristiano S.; Valette, Odile; González, Pablo Javier; Brondino, Carlos Dante; Moura, José J. G.; Moura, Isabel; Dolla, Alain; Rivas, Maria Gabriela
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Formate dehydrogenases (FDHs) are enzymes that catalyze the formate oxidation to carbon dioxide and that contain either Mo or W in a mononuclear form in the active site. In the present work, the influence of Mo and W salts on the production of FDH by Desulfovibrio alaskensis NCIMB 13491 was studied. Two different FDHs, one containing W (W-FDH) and a second incorporating either Mo or W (Mo/W-FDH), were purified. Both enzymes were isolated from cells grown in a medium supplemented with 1 μM molybdate, whereas only the W-FDH was purified from cells cultured in medium supplemented with 10 μM tungstate. We demonstrated that the genes encoding the Mo/W-FDH are strongly downregulated by W and slightly upregulated by Mo. Metal effects on the expression level of the genes encoding the W-FDH were less significant. Furthermore, the expression levels of the genes encoding proteins involved in molybdate and tungstate transport are downregulated under the experimental conditions evaluated in this work. The molecular and biochemical properties of these enzymes and the selective incorporation of either Mo or W are discussed.
Fil: Mota, Cristiano S.. Centro de Química Fina E Biotecnologia; Portugal
Fil: Valette, Odile. Centre National de la Recherche Scientifique; Francia
Fil: González, Pablo Javier. Centro de Química Fina E Biotecnologia; Portugal
Fil: Brondino, Carlos Dante. Universidad Nacional del Litoral; Argentina
Fil: Moura, José J. G.. Centro de Química Fina E Biotecnologia; Portugal
Fil: Moura, Isabel. Centro de Química Fina E Biotecnologia; Portugal
Fil: Dolla, Alain. Centre National de la Recherche Scientifique; Francia
Fil: Rivas, Maria Gabriela. Universidad Nacional del Litoral; Argentina - Materia
-
Formate dehydrogenases
Mo-enzymes
W-enzymes
sulfate reducing bacteria - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/96490
Ver los metadatos del registro completo
| id |
CONICETDig_00ccc95e53e46efee8cc9048903ec7c7 |
|---|---|
| oai_identifier_str |
oai:ri.conicet.gov.ar:11336/96490 |
| network_acronym_str |
CONICETDig |
| repository_id_str |
3498 |
| network_name_str |
CONICET Digital (CONICET) |
| spelling |
Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491Mota, Cristiano S.Valette, OdileGonzález, Pablo JavierBrondino, Carlos DanteMoura, José J. G.Moura, IsabelDolla, AlainRivas, Maria GabrielaFormate dehydrogenasesMo-enzymesW-enzymessulfate reducing bacteriahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Formate dehydrogenases (FDHs) are enzymes that catalyze the formate oxidation to carbon dioxide and that contain either Mo or W in a mononuclear form in the active site. In the present work, the influence of Mo and W salts on the production of FDH by Desulfovibrio alaskensis NCIMB 13491 was studied. Two different FDHs, one containing W (W-FDH) and a second incorporating either Mo or W (Mo/W-FDH), were purified. Both enzymes were isolated from cells grown in a medium supplemented with 1 μM molybdate, whereas only the W-FDH was purified from cells cultured in medium supplemented with 10 μM tungstate. We demonstrated that the genes encoding the Mo/W-FDH are strongly downregulated by W and slightly upregulated by Mo. Metal effects on the expression level of the genes encoding the W-FDH were less significant. Furthermore, the expression levels of the genes encoding proteins involved in molybdate and tungstate transport are downregulated under the experimental conditions evaluated in this work. The molecular and biochemical properties of these enzymes and the selective incorporation of either Mo or W are discussed.Fil: Mota, Cristiano S.. Centro de Química Fina E Biotecnologia; PortugalFil: Valette, Odile. Centre National de la Recherche Scientifique; FranciaFil: González, Pablo Javier. Centro de Química Fina E Biotecnologia; PortugalFil: Brondino, Carlos Dante. Universidad Nacional del Litoral; ArgentinaFil: Moura, José J. G.. Centro de Química Fina E Biotecnologia; PortugalFil: Moura, Isabel. Centro de Química Fina E Biotecnologia; PortugalFil: Dolla, Alain. Centre National de la Recherche Scientifique; FranciaFil: Rivas, Maria Gabriela. Universidad Nacional del Litoral; ArgentinaAmerican Society for Microbiology2011-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96490Mota, Cristiano S.; Valette, Odile; González, Pablo Javier; Brondino, Carlos Dante; Moura, José J. G.; et al.; Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491; American Society for Microbiology; Journal of Bacteriology; 193; 12; 6-2011; 2917-29230021-9193CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://jb.asm.org/content/193/12/2917info:eu-repo/semantics/altIdentifier/doi/10.1128/JB.01531-10info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:21:11Zoai:ri.conicet.gov.ar:11336/96490instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:21:11.795CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491 |
| title |
Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491 |
| spellingShingle |
Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491 Mota, Cristiano S. Formate dehydrogenases Mo-enzymes W-enzymes sulfate reducing bacteria |
| title_short |
Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491 |
| title_full |
Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491 |
| title_fullStr |
Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491 |
| title_full_unstemmed |
Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491 |
| title_sort |
Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491 |
| dc.creator.none.fl_str_mv |
Mota, Cristiano S. Valette, Odile González, Pablo Javier Brondino, Carlos Dante Moura, José J. G. Moura, Isabel Dolla, Alain Rivas, Maria Gabriela |
| author |
Mota, Cristiano S. |
| author_facet |
Mota, Cristiano S. Valette, Odile González, Pablo Javier Brondino, Carlos Dante Moura, José J. G. Moura, Isabel Dolla, Alain Rivas, Maria Gabriela |
| author_role |
author |
| author2 |
Valette, Odile González, Pablo Javier Brondino, Carlos Dante Moura, José J. G. Moura, Isabel Dolla, Alain Rivas, Maria Gabriela |
| author2_role |
author author author author author author author |
| dc.subject.none.fl_str_mv |
Formate dehydrogenases Mo-enzymes W-enzymes sulfate reducing bacteria |
| topic |
Formate dehydrogenases Mo-enzymes W-enzymes sulfate reducing bacteria |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Formate dehydrogenases (FDHs) are enzymes that catalyze the formate oxidation to carbon dioxide and that contain either Mo or W in a mononuclear form in the active site. In the present work, the influence of Mo and W salts on the production of FDH by Desulfovibrio alaskensis NCIMB 13491 was studied. Two different FDHs, one containing W (W-FDH) and a second incorporating either Mo or W (Mo/W-FDH), were purified. Both enzymes were isolated from cells grown in a medium supplemented with 1 μM molybdate, whereas only the W-FDH was purified from cells cultured in medium supplemented with 10 μM tungstate. We demonstrated that the genes encoding the Mo/W-FDH are strongly downregulated by W and slightly upregulated by Mo. Metal effects on the expression level of the genes encoding the W-FDH were less significant. Furthermore, the expression levels of the genes encoding proteins involved in molybdate and tungstate transport are downregulated under the experimental conditions evaluated in this work. The molecular and biochemical properties of these enzymes and the selective incorporation of either Mo or W are discussed. Fil: Mota, Cristiano S.. Centro de Química Fina E Biotecnologia; Portugal Fil: Valette, Odile. Centre National de la Recherche Scientifique; Francia Fil: González, Pablo Javier. Centro de Química Fina E Biotecnologia; Portugal Fil: Brondino, Carlos Dante. Universidad Nacional del Litoral; Argentina Fil: Moura, José J. G.. Centro de Química Fina E Biotecnologia; Portugal Fil: Moura, Isabel. Centro de Química Fina E Biotecnologia; Portugal Fil: Dolla, Alain. Centre National de la Recherche Scientifique; Francia Fil: Rivas, Maria Gabriela. Universidad Nacional del Litoral; Argentina |
| description |
Formate dehydrogenases (FDHs) are enzymes that catalyze the formate oxidation to carbon dioxide and that contain either Mo or W in a mononuclear form in the active site. In the present work, the influence of Mo and W salts on the production of FDH by Desulfovibrio alaskensis NCIMB 13491 was studied. Two different FDHs, one containing W (W-FDH) and a second incorporating either Mo or W (Mo/W-FDH), were purified. Both enzymes were isolated from cells grown in a medium supplemented with 1 μM molybdate, whereas only the W-FDH was purified from cells cultured in medium supplemented with 10 μM tungstate. We demonstrated that the genes encoding the Mo/W-FDH are strongly downregulated by W and slightly upregulated by Mo. Metal effects on the expression level of the genes encoding the W-FDH were less significant. Furthermore, the expression levels of the genes encoding proteins involved in molybdate and tungstate transport are downregulated under the experimental conditions evaluated in this work. The molecular and biochemical properties of these enzymes and the selective incorporation of either Mo or W are discussed. |
| publishDate |
2011 |
| dc.date.none.fl_str_mv |
2011-06 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/96490 Mota, Cristiano S.; Valette, Odile; González, Pablo Javier; Brondino, Carlos Dante; Moura, José J. G.; et al.; Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491; American Society for Microbiology; Journal of Bacteriology; 193; 12; 6-2011; 2917-2923 0021-9193 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/96490 |
| identifier_str_mv |
Mota, Cristiano S.; Valette, Odile; González, Pablo Javier; Brondino, Carlos Dante; Moura, José J. G.; et al.; Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491; American Society for Microbiology; Journal of Bacteriology; 193; 12; 6-2011; 2917-2923 0021-9193 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://jb.asm.org/content/193/12/2917 info:eu-repo/semantics/altIdentifier/doi/10.1128/JB.01531-10 |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
| eu_rights_str_mv |
openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
| dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
American Society for Microbiology |
| publisher.none.fl_str_mv |
American Society for Microbiology |
| dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
| reponame_str |
CONICET Digital (CONICET) |
| collection |
CONICET Digital (CONICET) |
| instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
| repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
| repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
| _version_ |
1846083360484491264 |
| score |
13.22299 |