Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491

Autores
Mota, Cristiano S.; Valette, Odile; González, Pablo Javier; Brondino, Carlos Dante; Moura, José J. G.; Moura, Isabel; Dolla, Alain; Rivas, Maria Gabriela
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Formate dehydrogenases (FDHs) are enzymes that catalyze the formate oxidation to carbon dioxide and that contain either Mo or W in a mononuclear form in the active site. In the present work, the influence of Mo and W salts on the production of FDH by Desulfovibrio alaskensis NCIMB 13491 was studied. Two different FDHs, one containing W (W-FDH) and a second incorporating either Mo or W (Mo/W-FDH), were purified. Both enzymes were isolated from cells grown in a medium supplemented with 1 μM molybdate, whereas only the W-FDH was purified from cells cultured in medium supplemented with 10 μM tungstate. We demonstrated that the genes encoding the Mo/W-FDH are strongly downregulated by W and slightly upregulated by Mo. Metal effects on the expression level of the genes encoding the W-FDH were less significant. Furthermore, the expression levels of the genes encoding proteins involved in molybdate and tungstate transport are downregulated under the experimental conditions evaluated in this work. The molecular and biochemical properties of these enzymes and the selective incorporation of either Mo or W are discussed.
Fil: Mota, Cristiano S.. Centro de Química Fina E Biotecnologia; Portugal
Fil: Valette, Odile. Centre National de la Recherche Scientifique; Francia
Fil: González, Pablo Javier. Centro de Química Fina E Biotecnologia; Portugal
Fil: Brondino, Carlos Dante. Universidad Nacional del Litoral; Argentina
Fil: Moura, José J. G.. Centro de Química Fina E Biotecnologia; Portugal
Fil: Moura, Isabel. Centro de Química Fina E Biotecnologia; Portugal
Fil: Dolla, Alain. Centre National de la Recherche Scientifique; Francia
Fil: Rivas, Maria Gabriela. Universidad Nacional del Litoral; Argentina
Materia
Formate dehydrogenases
Mo-enzymes
W-enzymes
sulfate reducing bacteria
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96490

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network_name_str CONICET Digital (CONICET)
spelling Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491Mota, Cristiano S.Valette, OdileGonzález, Pablo JavierBrondino, Carlos DanteMoura, José J. G.Moura, IsabelDolla, AlainRivas, Maria GabrielaFormate dehydrogenasesMo-enzymesW-enzymessulfate reducing bacteriahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Formate dehydrogenases (FDHs) are enzymes that catalyze the formate oxidation to carbon dioxide and that contain either Mo or W in a mononuclear form in the active site. In the present work, the influence of Mo and W salts on the production of FDH by Desulfovibrio alaskensis NCIMB 13491 was studied. Two different FDHs, one containing W (W-FDH) and a second incorporating either Mo or W (Mo/W-FDH), were purified. Both enzymes were isolated from cells grown in a medium supplemented with 1 μM molybdate, whereas only the W-FDH was purified from cells cultured in medium supplemented with 10 μM tungstate. We demonstrated that the genes encoding the Mo/W-FDH are strongly downregulated by W and slightly upregulated by Mo. Metal effects on the expression level of the genes encoding the W-FDH were less significant. Furthermore, the expression levels of the genes encoding proteins involved in molybdate and tungstate transport are downregulated under the experimental conditions evaluated in this work. The molecular and biochemical properties of these enzymes and the selective incorporation of either Mo or W are discussed.Fil: Mota, Cristiano S.. Centro de Química Fina E Biotecnologia; PortugalFil: Valette, Odile. Centre National de la Recherche Scientifique; FranciaFil: González, Pablo Javier. Centro de Química Fina E Biotecnologia; PortugalFil: Brondino, Carlos Dante. Universidad Nacional del Litoral; ArgentinaFil: Moura, José J. G.. Centro de Química Fina E Biotecnologia; PortugalFil: Moura, Isabel. Centro de Química Fina E Biotecnologia; PortugalFil: Dolla, Alain. Centre National de la Recherche Scientifique; FranciaFil: Rivas, Maria Gabriela. Universidad Nacional del Litoral; ArgentinaAmerican Society for Microbiology2011-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96490Mota, Cristiano S.; Valette, Odile; González, Pablo Javier; Brondino, Carlos Dante; Moura, José J. G.; et al.; Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491; American Society for Microbiology; Journal of Bacteriology; 193; 12; 6-2011; 2917-29230021-9193CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://jb.asm.org/content/193/12/2917info:eu-repo/semantics/altIdentifier/doi/10.1128/JB.01531-10info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:21:11Zoai:ri.conicet.gov.ar:11336/96490instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:21:11.795CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491
title Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491
spellingShingle Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491
Mota, Cristiano S.
Formate dehydrogenases
Mo-enzymes
W-enzymes
sulfate reducing bacteria
title_short Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491
title_full Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491
title_fullStr Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491
title_full_unstemmed Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491
title_sort Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491
dc.creator.none.fl_str_mv Mota, Cristiano S.
Valette, Odile
González, Pablo Javier
Brondino, Carlos Dante
Moura, José J. G.
Moura, Isabel
Dolla, Alain
Rivas, Maria Gabriela
author Mota, Cristiano S.
author_facet Mota, Cristiano S.
Valette, Odile
González, Pablo Javier
Brondino, Carlos Dante
Moura, José J. G.
Moura, Isabel
Dolla, Alain
Rivas, Maria Gabriela
author_role author
author2 Valette, Odile
González, Pablo Javier
Brondino, Carlos Dante
Moura, José J. G.
Moura, Isabel
Dolla, Alain
Rivas, Maria Gabriela
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Formate dehydrogenases
Mo-enzymes
W-enzymes
sulfate reducing bacteria
topic Formate dehydrogenases
Mo-enzymes
W-enzymes
sulfate reducing bacteria
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Formate dehydrogenases (FDHs) are enzymes that catalyze the formate oxidation to carbon dioxide and that contain either Mo or W in a mononuclear form in the active site. In the present work, the influence of Mo and W salts on the production of FDH by Desulfovibrio alaskensis NCIMB 13491 was studied. Two different FDHs, one containing W (W-FDH) and a second incorporating either Mo or W (Mo/W-FDH), were purified. Both enzymes were isolated from cells grown in a medium supplemented with 1 μM molybdate, whereas only the W-FDH was purified from cells cultured in medium supplemented with 10 μM tungstate. We demonstrated that the genes encoding the Mo/W-FDH are strongly downregulated by W and slightly upregulated by Mo. Metal effects on the expression level of the genes encoding the W-FDH were less significant. Furthermore, the expression levels of the genes encoding proteins involved in molybdate and tungstate transport are downregulated under the experimental conditions evaluated in this work. The molecular and biochemical properties of these enzymes and the selective incorporation of either Mo or W are discussed.
Fil: Mota, Cristiano S.. Centro de Química Fina E Biotecnologia; Portugal
Fil: Valette, Odile. Centre National de la Recherche Scientifique; Francia
Fil: González, Pablo Javier. Centro de Química Fina E Biotecnologia; Portugal
Fil: Brondino, Carlos Dante. Universidad Nacional del Litoral; Argentina
Fil: Moura, José J. G.. Centro de Química Fina E Biotecnologia; Portugal
Fil: Moura, Isabel. Centro de Química Fina E Biotecnologia; Portugal
Fil: Dolla, Alain. Centre National de la Recherche Scientifique; Francia
Fil: Rivas, Maria Gabriela. Universidad Nacional del Litoral; Argentina
description Formate dehydrogenases (FDHs) are enzymes that catalyze the formate oxidation to carbon dioxide and that contain either Mo or W in a mononuclear form in the active site. In the present work, the influence of Mo and W salts on the production of FDH by Desulfovibrio alaskensis NCIMB 13491 was studied. Two different FDHs, one containing W (W-FDH) and a second incorporating either Mo or W (Mo/W-FDH), were purified. Both enzymes were isolated from cells grown in a medium supplemented with 1 μM molybdate, whereas only the W-FDH was purified from cells cultured in medium supplemented with 10 μM tungstate. We demonstrated that the genes encoding the Mo/W-FDH are strongly downregulated by W and slightly upregulated by Mo. Metal effects on the expression level of the genes encoding the W-FDH were less significant. Furthermore, the expression levels of the genes encoding proteins involved in molybdate and tungstate transport are downregulated under the experimental conditions evaluated in this work. The molecular and biochemical properties of these enzymes and the selective incorporation of either Mo or W are discussed.
publishDate 2011
dc.date.none.fl_str_mv 2011-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96490
Mota, Cristiano S.; Valette, Odile; González, Pablo Javier; Brondino, Carlos Dante; Moura, José J. G.; et al.; Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491; American Society for Microbiology; Journal of Bacteriology; 193; 12; 6-2011; 2917-2923
0021-9193
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96490
identifier_str_mv Mota, Cristiano S.; Valette, Odile; González, Pablo Javier; Brondino, Carlos Dante; Moura, José J. G.; et al.; Effects of molybdate and tungstate on expression levels and biochemical characteristics of formate dehydrogenases produced by Desulfovibrio alaskensis NCIMB 13491; American Society for Microbiology; Journal of Bacteriology; 193; 12; 6-2011; 2917-2923
0021-9193
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jb.asm.org/content/193/12/2917
info:eu-repo/semantics/altIdentifier/doi/10.1128/JB.01531-10
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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