Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability

Autores
Gurdo, Nicolás; Novelli Poisson, Guido Fernando; Juárez, Angela Beatriz; Rios, Maria del Carmen; Galvagno, Miguel Angel
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: To investigate multiple tolerance of Saccharomyces cerevisiae obtained through a laboratory strategy of adaptive evolution in acetic acid, its relation with enzymatic ROS detoxification and bioethanol 2G production. Methods and Results: After adaptive evolution in acetic acid, a clone (Y8A) was selected for its tolerance to high acetic acid concentrations (13 g l−1) in batch cultures. Y8A was resistant to multiple stresses: osmotic, thermic, oxidative, saline, ethanol, organic acid, phenolic compounds and slow freeze-thawing cycles. Also, Y8A was able to maintain redox homeostasis under oxidative stress, whereas the isogenic parental strain (Y8) could not, indicating higher basal activity levels of antioxidative enzyme Catalase (CAT) and Gluthatione S-transferase (GST) in Y8A. Y8A reached higher bioethanol levels in a fermentation medium containing up to 8 g l−1 of acetic acid when compared to parental strain Y8. Conclusions: A multiple-stress-tolerant clone was obtained using adaptive evolution in acetic acid. Stress cross-tolerance could be explained by its enzymatic antioxidative capacity, namely CAT and GST. Significance and Impact of the Study: We demonstrate that adaptive evolution used in S. cerevisiae was a useful strategy to obtain a yeast clone tolerant to multiple stresses. At the same time, our findings support the idea that tolerance to oxidative stress is the common basis for stress cotolerance, which is related to an increase in the specific enzymes CAT and GST but not in Superoxide dismutase, emphasizing the fact that detoxification of H2O2 and not O2˙ is a key condition for multiple stress tolerance in S. cerevisiae.
Fil: Gurdo, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Novelli Poisson, Guido Fernando. Universidad de Buenos Aires; Argentina
Fil: Juárez, Angela Beatriz. Universidad de Buenos Aires; Argentina
Fil: Rios de Molina, M.C.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Galvagno, Miguel Angel. Universidad de Buenos Aires; Argentina
Materia
ACETIC ACID
ADAPTIVE EVOLUTION
ANTIOXIDATIVE ENZYMES
BIOETHANOL 2G PRODUCTION
MULTIPLE TOLERANCE
ROBUSTNESS
SACCHAROMYCES CEREVISIAE
YEAST
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94079
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/94079
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant abilityGurdo, NicolásNovelli Poisson, Guido FernandoJuárez, Angela BeatrizRios, Maria del CarmenGalvagno, Miguel AngelACETIC ACIDADAPTIVE EVOLUTIONANTIOXIDATIVE ENZYMESBIOETHANOL 2G PRODUCTIONMULTIPLE TOLERANCEROBUSTNESSSACCHAROMYCES CEREVISIAEYEASThttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Aims: To investigate multiple tolerance of Saccharomyces cerevisiae obtained through a laboratory strategy of adaptive evolution in acetic acid, its relation with enzymatic ROS detoxification and bioethanol 2G production. Methods and Results: After adaptive evolution in acetic acid, a clone (Y8A) was selected for its tolerance to high acetic acid concentrations (13 g l−1) in batch cultures. Y8A was resistant to multiple stresses: osmotic, thermic, oxidative, saline, ethanol, organic acid, phenolic compounds and slow freeze-thawing cycles. Also, Y8A was able to maintain redox homeostasis under oxidative stress, whereas the isogenic parental strain (Y8) could not, indicating higher basal activity levels of antioxidative enzyme Catalase (CAT) and Gluthatione S-transferase (GST) in Y8A. Y8A reached higher bioethanol levels in a fermentation medium containing up to 8 g l−1 of acetic acid when compared to parental strain Y8. Conclusions: A multiple-stress-tolerant clone was obtained using adaptive evolution in acetic acid. Stress cross-tolerance could be explained by its enzymatic antioxidative capacity, namely CAT and GST. Significance and Impact of the Study: We demonstrate that adaptive evolution used in S. cerevisiae was a useful strategy to obtain a yeast clone tolerant to multiple stresses. At the same time, our findings support the idea that tolerance to oxidative stress is the common basis for stress cotolerance, which is related to an increase in the specific enzymes CAT and GST but not in Superoxide dismutase, emphasizing the fact that detoxification of H2O2 and not O2˙ is a key condition for multiple stress tolerance in S. cerevisiae.Fil: Gurdo, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Novelli Poisson, Guido Fernando. Universidad de Buenos Aires; ArgentinaFil: Juárez, Angela Beatriz. Universidad de Buenos Aires; ArgentinaFil: Rios de Molina, M.C.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Galvagno, Miguel Angel. Universidad de Buenos Aires; ArgentinaWiley Blackwell Publishing, Inc2018-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94079Gurdo, Nicolás; Novelli Poisson, Guido Fernando; Juárez, Angela Beatriz; Rios, Maria del Carmen; Galvagno, Miguel Angel; Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 125; 3; 9-2018; 766-7761364-5072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/jam.13917info:eu-repo/semantics/altIdentifier/doi/10.1111/jam.13917info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:49:03Zoai:ri.conicet.gov.ar:11336/94079instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:49:04.042CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability
title Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability
spellingShingle Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability
Gurdo, Nicolás
ACETIC ACID
ADAPTIVE EVOLUTION
ANTIOXIDATIVE ENZYMES
BIOETHANOL 2G PRODUCTION
MULTIPLE TOLERANCE
ROBUSTNESS
SACCHAROMYCES CEREVISIAE
YEAST
title_short Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability
title_full Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability
title_fullStr Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability
title_full_unstemmed Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability
title_sort Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability
dc.creator.none.fl_str_mv Gurdo, Nicolás
Novelli Poisson, Guido Fernando
Juárez, Angela Beatriz
Rios, Maria del Carmen
Galvagno, Miguel Angel
author Gurdo, Nicolás
author_facet Gurdo, Nicolás
Novelli Poisson, Guido Fernando
Juárez, Angela Beatriz
Rios, Maria del Carmen
Galvagno, Miguel Angel
author_role author
author2 Novelli Poisson, Guido Fernando
Juárez, Angela Beatriz
Rios, Maria del Carmen
Galvagno, Miguel Angel
author2_role author
author
author
author
dc.subject.none.fl_str_mv ACETIC ACID
ADAPTIVE EVOLUTION
ANTIOXIDATIVE ENZYMES
BIOETHANOL 2G PRODUCTION
MULTIPLE TOLERANCE
ROBUSTNESS
SACCHAROMYCES CEREVISIAE
YEAST
topic ACETIC ACID
ADAPTIVE EVOLUTION
ANTIOXIDATIVE ENZYMES
BIOETHANOL 2G PRODUCTION
MULTIPLE TOLERANCE
ROBUSTNESS
SACCHAROMYCES CEREVISIAE
YEAST
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Aims: To investigate multiple tolerance of Saccharomyces cerevisiae obtained through a laboratory strategy of adaptive evolution in acetic acid, its relation with enzymatic ROS detoxification and bioethanol 2G production. Methods and Results: After adaptive evolution in acetic acid, a clone (Y8A) was selected for its tolerance to high acetic acid concentrations (13 g l−1) in batch cultures. Y8A was resistant to multiple stresses: osmotic, thermic, oxidative, saline, ethanol, organic acid, phenolic compounds and slow freeze-thawing cycles. Also, Y8A was able to maintain redox homeostasis under oxidative stress, whereas the isogenic parental strain (Y8) could not, indicating higher basal activity levels of antioxidative enzyme Catalase (CAT) and Gluthatione S-transferase (GST) in Y8A. Y8A reached higher bioethanol levels in a fermentation medium containing up to 8 g l−1 of acetic acid when compared to parental strain Y8. Conclusions: A multiple-stress-tolerant clone was obtained using adaptive evolution in acetic acid. Stress cross-tolerance could be explained by its enzymatic antioxidative capacity, namely CAT and GST. Significance and Impact of the Study: We demonstrate that adaptive evolution used in S. cerevisiae was a useful strategy to obtain a yeast clone tolerant to multiple stresses. At the same time, our findings support the idea that tolerance to oxidative stress is the common basis for stress cotolerance, which is related to an increase in the specific enzymes CAT and GST but not in Superoxide dismutase, emphasizing the fact that detoxification of H2O2 and not O2˙ is a key condition for multiple stress tolerance in S. cerevisiae.
Fil: Gurdo, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Novelli Poisson, Guido Fernando. Universidad de Buenos Aires; Argentina
Fil: Juárez, Angela Beatriz. Universidad de Buenos Aires; Argentina
Fil: Rios de Molina, M.C.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Galvagno, Miguel Angel. Universidad de Buenos Aires; Argentina
description Aims: To investigate multiple tolerance of Saccharomyces cerevisiae obtained through a laboratory strategy of adaptive evolution in acetic acid, its relation with enzymatic ROS detoxification and bioethanol 2G production. Methods and Results: After adaptive evolution in acetic acid, a clone (Y8A) was selected for its tolerance to high acetic acid concentrations (13 g l−1) in batch cultures. Y8A was resistant to multiple stresses: osmotic, thermic, oxidative, saline, ethanol, organic acid, phenolic compounds and slow freeze-thawing cycles. Also, Y8A was able to maintain redox homeostasis under oxidative stress, whereas the isogenic parental strain (Y8) could not, indicating higher basal activity levels of antioxidative enzyme Catalase (CAT) and Gluthatione S-transferase (GST) in Y8A. Y8A reached higher bioethanol levels in a fermentation medium containing up to 8 g l−1 of acetic acid when compared to parental strain Y8. Conclusions: A multiple-stress-tolerant clone was obtained using adaptive evolution in acetic acid. Stress cross-tolerance could be explained by its enzymatic antioxidative capacity, namely CAT and GST. Significance and Impact of the Study: We demonstrate that adaptive evolution used in S. cerevisiae was a useful strategy to obtain a yeast clone tolerant to multiple stresses. At the same time, our findings support the idea that tolerance to oxidative stress is the common basis for stress cotolerance, which is related to an increase in the specific enzymes CAT and GST but not in Superoxide dismutase, emphasizing the fact that detoxification of H2O2 and not O2˙ is a key condition for multiple stress tolerance in S. cerevisiae.
publishDate 2018
dc.date.none.fl_str_mv 2018-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94079
Gurdo, Nicolás; Novelli Poisson, Guido Fernando; Juárez, Angela Beatriz; Rios, Maria del Carmen; Galvagno, Miguel Angel; Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 125; 3; 9-2018; 766-776
1364-5072
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94079
identifier_str_mv Gurdo, Nicolás; Novelli Poisson, Guido Fernando; Juárez, Angela Beatriz; Rios, Maria del Carmen; Galvagno, Miguel Angel; Improved robustness of an ethanologenic yeast strain through adaptive evolution in acetic acid is associated with its enzymatic antioxidant ability; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 125; 3; 9-2018; 766-776
1364-5072
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/jam.13917
info:eu-repo/semantics/altIdentifier/doi/10.1111/jam.13917
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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