Isolation and characterization of Saccharomyces species for bioethanol production from sugarcane molasses: Studies of scale up in bioreactor

Autores
Muruaga, María Laura; Gianni de Carvalho, Katia; Domínguez, José Manuel; Oliveira, Ricardo Pinheiro de Souza; Perotti, Nora Ines
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Decreases in oil reserves and gas fields around all over the world justify the deepening of studies to render viable the larger-scale use of new energy sources. Therefore, the use of microorganisms to convert sugars into ethanol is a feasible process to be performed in a short period of time and at low costs. In this context, this study aimed to select ethanol-producing yeasts, after isolating samples in molasses obtained from companies in the Province of Tucumán (Argentina) and grapes obtained from farms located in Cafayate (Salta, Argentina). Among the twenty-nine samples studied A2, A10 and A11 isolates showed higher ethanol productions of 12.87; 13.64 and 13.46% respectively. A2 showed a homogeneous growth meanwhile the growth of strains A10 and A11 was flocculent. Molecular taxonomic characterization of these isolates showed a percentage of similarity of 100% with the strain Saccharomyces cerevisiae. The behavior of the non-flocculent A2 strain at laboratory scale was faster using a sugarcane molasses based medium, reaching 11.36% ethanol without adding nutrients and other growth factors, probably because its disperse form facilitates the transfer of nutrients and products. These values were improved to 12.02% when the process was scaled up to a 10L bioreactor. All these studies allowed concluding that S. cerevisiae A2 strain is a promising microorganism for the production of bioethanol with potential environmental, energy and economic benefits to be projected into industrial scale.
Fil: Muruaga, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
Fil: Gianni de Carvalho, Katia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
Fil: Domínguez, José Manuel. Universidad de Vigo. Facultad de Ciencias de Ourense; España
Fil: Oliveira, Ricardo Pinheiro de Souza. Universidade de Sao Paulo; Brasil
Fil: Perotti, Nora Ines. Universidad Nacional de Tucuman. Facultad de Cs.exactas y Tecnología. Departamento de Ingeniería En Procesos y Gestion Industrial. Laboratorio de Biotecnología; Argentina
Materia
Bioethanol
Bioreator
Saccharomyces Cerevisiae
Sugarcane Molasses
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37582
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Isolation and characterization of Saccharomyces species for bioethanol production from sugarcane molasses: Studies of scale up in bioreactorMuruaga, María LauraGianni de Carvalho, KatiaDomínguez, José ManuelOliveira, Ricardo Pinheiro de SouzaPerotti, Nora InesBioethanolBioreatorSaccharomyces CerevisiaeSugarcane Molasseshttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Decreases in oil reserves and gas fields around all over the world justify the deepening of studies to render viable the larger-scale use of new energy sources. Therefore, the use of microorganisms to convert sugars into ethanol is a feasible process to be performed in a short period of time and at low costs. In this context, this study aimed to select ethanol-producing yeasts, after isolating samples in molasses obtained from companies in the Province of Tucumán (Argentina) and grapes obtained from farms located in Cafayate (Salta, Argentina). Among the twenty-nine samples studied A2, A10 and A11 isolates showed higher ethanol productions of 12.87; 13.64 and 13.46% respectively. A2 showed a homogeneous growth meanwhile the growth of strains A10 and A11 was flocculent. Molecular taxonomic characterization of these isolates showed a percentage of similarity of 100% with the strain Saccharomyces cerevisiae. The behavior of the non-flocculent A2 strain at laboratory scale was faster using a sugarcane molasses based medium, reaching 11.36% ethanol without adding nutrients and other growth factors, probably because its disperse form facilitates the transfer of nutrients and products. These values were improved to 12.02% when the process was scaled up to a 10L bioreactor. All these studies allowed concluding that S. cerevisiae A2 strain is a promising microorganism for the production of bioethanol with potential environmental, energy and economic benefits to be projected into industrial scale.Fil: Muruaga, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; ArgentinaFil: Gianni de Carvalho, Katia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; ArgentinaFil: Domínguez, José Manuel. Universidad de Vigo. Facultad de Ciencias de Ourense; EspañaFil: Oliveira, Ricardo Pinheiro de Souza. Universidade de Sao Paulo; BrasilFil: Perotti, Nora Ines. Universidad Nacional de Tucuman. Facultad de Cs.exactas y Tecnología. Departamento de Ingeniería En Procesos y Gestion Industrial. Laboratorio de Biotecnología; ArgentinaPergamon-Elsevier Science Ltd2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37582Muruaga, María Laura; Gianni de Carvalho, Katia; Domínguez, José Manuel; Oliveira, Ricardo Pinheiro de Souza ; Perotti, Nora Ines; Isolation and characterization of Saccharomyces species for bioethanol production from sugarcane molasses: Studies of scale up in bioreactor; Pergamon-Elsevier Science Ltd; Renewable Energy; 85; 1-2016; 649-6560960-1481CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.renene.2015.07.008info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0960148115301051info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:50Zoai:ri.conicet.gov.ar:11336/37582instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:50.671CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation and characterization of Saccharomyces species for bioethanol production from sugarcane molasses: Studies of scale up in bioreactor
title Isolation and characterization of Saccharomyces species for bioethanol production from sugarcane molasses: Studies of scale up in bioreactor
spellingShingle Isolation and characterization of Saccharomyces species for bioethanol production from sugarcane molasses: Studies of scale up in bioreactor
Muruaga, María Laura
Bioethanol
Bioreator
Saccharomyces Cerevisiae
Sugarcane Molasses
title_short Isolation and characterization of Saccharomyces species for bioethanol production from sugarcane molasses: Studies of scale up in bioreactor
title_full Isolation and characterization of Saccharomyces species for bioethanol production from sugarcane molasses: Studies of scale up in bioreactor
title_fullStr Isolation and characterization of Saccharomyces species for bioethanol production from sugarcane molasses: Studies of scale up in bioreactor
title_full_unstemmed Isolation and characterization of Saccharomyces species for bioethanol production from sugarcane molasses: Studies of scale up in bioreactor
title_sort Isolation and characterization of Saccharomyces species for bioethanol production from sugarcane molasses: Studies of scale up in bioreactor
dc.creator.none.fl_str_mv Muruaga, María Laura
Gianni de Carvalho, Katia
Domínguez, José Manuel
Oliveira, Ricardo Pinheiro de Souza
Perotti, Nora Ines
author Muruaga, María Laura
author_facet Muruaga, María Laura
Gianni de Carvalho, Katia
Domínguez, José Manuel
Oliveira, Ricardo Pinheiro de Souza
Perotti, Nora Ines
author_role author
author2 Gianni de Carvalho, Katia
Domínguez, José Manuel
Oliveira, Ricardo Pinheiro de Souza
Perotti, Nora Ines
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bioethanol
Bioreator
Saccharomyces Cerevisiae
Sugarcane Molasses
topic Bioethanol
Bioreator
Saccharomyces Cerevisiae
Sugarcane Molasses
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Decreases in oil reserves and gas fields around all over the world justify the deepening of studies to render viable the larger-scale use of new energy sources. Therefore, the use of microorganisms to convert sugars into ethanol is a feasible process to be performed in a short period of time and at low costs. In this context, this study aimed to select ethanol-producing yeasts, after isolating samples in molasses obtained from companies in the Province of Tucumán (Argentina) and grapes obtained from farms located in Cafayate (Salta, Argentina). Among the twenty-nine samples studied A2, A10 and A11 isolates showed higher ethanol productions of 12.87; 13.64 and 13.46% respectively. A2 showed a homogeneous growth meanwhile the growth of strains A10 and A11 was flocculent. Molecular taxonomic characterization of these isolates showed a percentage of similarity of 100% with the strain Saccharomyces cerevisiae. The behavior of the non-flocculent A2 strain at laboratory scale was faster using a sugarcane molasses based medium, reaching 11.36% ethanol without adding nutrients and other growth factors, probably because its disperse form facilitates the transfer of nutrients and products. These values were improved to 12.02% when the process was scaled up to a 10L bioreactor. All these studies allowed concluding that S. cerevisiae A2 strain is a promising microorganism for the production of bioethanol with potential environmental, energy and economic benefits to be projected into industrial scale.
Fil: Muruaga, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
Fil: Gianni de Carvalho, Katia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
Fil: Domínguez, José Manuel. Universidad de Vigo. Facultad de Ciencias de Ourense; España
Fil: Oliveira, Ricardo Pinheiro de Souza. Universidade de Sao Paulo; Brasil
Fil: Perotti, Nora Ines. Universidad Nacional de Tucuman. Facultad de Cs.exactas y Tecnología. Departamento de Ingeniería En Procesos y Gestion Industrial. Laboratorio de Biotecnología; Argentina
description Decreases in oil reserves and gas fields around all over the world justify the deepening of studies to render viable the larger-scale use of new energy sources. Therefore, the use of microorganisms to convert sugars into ethanol is a feasible process to be performed in a short period of time and at low costs. In this context, this study aimed to select ethanol-producing yeasts, after isolating samples in molasses obtained from companies in the Province of Tucumán (Argentina) and grapes obtained from farms located in Cafayate (Salta, Argentina). Among the twenty-nine samples studied A2, A10 and A11 isolates showed higher ethanol productions of 12.87; 13.64 and 13.46% respectively. A2 showed a homogeneous growth meanwhile the growth of strains A10 and A11 was flocculent. Molecular taxonomic characterization of these isolates showed a percentage of similarity of 100% with the strain Saccharomyces cerevisiae. The behavior of the non-flocculent A2 strain at laboratory scale was faster using a sugarcane molasses based medium, reaching 11.36% ethanol without adding nutrients and other growth factors, probably because its disperse form facilitates the transfer of nutrients and products. These values were improved to 12.02% when the process was scaled up to a 10L bioreactor. All these studies allowed concluding that S. cerevisiae A2 strain is a promising microorganism for the production of bioethanol with potential environmental, energy and economic benefits to be projected into industrial scale.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37582
Muruaga, María Laura; Gianni de Carvalho, Katia; Domínguez, José Manuel; Oliveira, Ricardo Pinheiro de Souza ; Perotti, Nora Ines; Isolation and characterization of Saccharomyces species for bioethanol production from sugarcane molasses: Studies of scale up in bioreactor; Pergamon-Elsevier Science Ltd; Renewable Energy; 85; 1-2016; 649-656
0960-1481
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37582
identifier_str_mv Muruaga, María Laura; Gianni de Carvalho, Katia; Domínguez, José Manuel; Oliveira, Ricardo Pinheiro de Souza ; Perotti, Nora Ines; Isolation and characterization of Saccharomyces species for bioethanol production from sugarcane molasses: Studies of scale up in bioreactor; Pergamon-Elsevier Science Ltd; Renewable Energy; 85; 1-2016; 649-656
0960-1481
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.renene.2015.07.008
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0960148115301051
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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