Prevalence of Lactobacillus plantarum and Oenococcus oeni during spontaneous malolactic fermentation in Patagonian red wines revealed by polymerase chain reaction-denaturing gradie...

Autores
Valdes la Hens, Danay; Bravo Ferrada, Barbara Mercedes; Delfederico, Lucrecia; Caballero, Adriana Catalina; Semorile, Liliana Carmen
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background and Aims:Information regarding the composition and dynamics of microbial communities through-out winemaking is always valuable to control the process and contributes to improving the quality of wine. The aimsof this work were to analyse the diversity of lactic acid bacteria (LAB) species present during the spontaneousmalolactic fermentation (MLF) of Patagonian red wines, and to isolate and identify native LAB species.Methods and Results:Samples of Merlot and Pinot Noir wines were collected during three vintages at onecommercial cellar. Through polymerase chain reaction (PCR)-rpoB/denaturing gradient gel electrophoresis (DGGE),it was possible to identify 15 LAB species and through PCR-16S rRNAV3 region/DGGE, 11 LAB species, duringspontaneous MLF of these wines, mostly,Oenococcus oeniandLactobacillus plantarum. These two species were alsolargely recovered by culture and analysed by the random amplified polymorphic DNA–PCR technique. Isolates ofthese two LAB species exhibited considerable genotypic heterogeneity.Conclusions:The prevalence ofO. oeniandLb. plantarumin all samples suggests that both species are involved inleading the spontaneous MLF of these Patagonian wines. The analysis of the LAB microbiota in wine by PCR-DGGEusing two gene regions enabled addition to the number of species that can usually be detected during MLF.Significance of the Study:The study offered a more complete knowledge of the LAB community present in winesin a cellar in the Argentine Patagonia during the spontaneous MLF. Our findings are particularly relevant, becausethey contribute to the possibility of designing an MLF starter composed of native strains ofLb. plantarumandO. oeni
Fil: Valdes la Hens, Danay. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
Fil: Bravo Ferrada, Barbara Mercedes. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
Fil: Delfederico, Lucrecia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
Fil: Caballero, Adriana Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. IDEPA - Subsede San Antonio Oeste; Argentina. Universidad Nacional del Comahue; Argentina
Fil: Semorile, Liliana Carmen. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
Materia
Lactobacillus Plantarum
Malolactic Fermentation
Oenococcus Oeni
Patagonian Red Wine
Pcr-Dgge
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36472
Acceder
id CONICETDig_d1d4cbe2cde0bdd9dfffdf8547f60b94
oai_identifier_str oai:ri.conicet.gov.ar:11336/36472
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Prevalence of Lactobacillus plantarum and Oenococcus oeni during spontaneous malolactic fermentation in Patagonian red wines revealed by polymerase chain reaction-denaturing gradient gel electrophoresis with two targeted genesValdes la Hens, DanayBravo Ferrada, Barbara MercedesDelfederico, LucreciaCaballero, Adriana CatalinaSemorile, Liliana CarmenLactobacillus PlantarumMalolactic FermentationOenococcus OeniPatagonian Red WinePcr-Dggehttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Background and Aims:Information regarding the composition and dynamics of microbial communities through-out winemaking is always valuable to control the process and contributes to improving the quality of wine. The aimsof this work were to analyse the diversity of lactic acid bacteria (LAB) species present during the spontaneousmalolactic fermentation (MLF) of Patagonian red wines, and to isolate and identify native LAB species.Methods and Results:Samples of Merlot and Pinot Noir wines were collected during three vintages at onecommercial cellar. Through polymerase chain reaction (PCR)-rpoB/denaturing gradient gel electrophoresis (DGGE),it was possible to identify 15 LAB species and through PCR-16S rRNAV3 region/DGGE, 11 LAB species, duringspontaneous MLF of these wines, mostly,Oenococcus oeniandLactobacillus plantarum. These two species were alsolargely recovered by culture and analysed by the random amplified polymorphic DNA–PCR technique. Isolates ofthese two LAB species exhibited considerable genotypic heterogeneity.Conclusions:The prevalence ofO. oeniandLb. plantarumin all samples suggests that both species are involved inleading the spontaneous MLF of these Patagonian wines. The analysis of the LAB microbiota in wine by PCR-DGGEusing two gene regions enabled addition to the number of species that can usually be detected during MLF.Significance of the Study:The study offered a more complete knowledge of the LAB community present in winesin a cellar in the Argentine Patagonia during the spontaneous MLF. Our findings are particularly relevant, becausethey contribute to the possibility of designing an MLF starter composed of native strains ofLb. plantarumandO. oeniFil: Valdes la Hens, Danay. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; ArgentinaFil: Bravo Ferrada, Barbara Mercedes. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; ArgentinaFil: Delfederico, Lucrecia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; ArgentinaFil: Caballero, Adriana Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. IDEPA - Subsede San Antonio Oeste; Argentina. Universidad Nacional del Comahue; ArgentinaFil: Semorile, Liliana Carmen. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; ArgentinaWiley Blackwell Publishing, Inc2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36472Valdes la Hens, Danay; Bravo Ferrada, Barbara Mercedes; Delfederico, Lucrecia; Caballero, Adriana Catalina; Semorile, Liliana Carmen; Prevalence of Lactobacillus plantarum and Oenococcus oeni during spontaneous malolactic fermentation in Patagonian red wines revealed by polymerase chain reaction-denaturing gradient gel electrophoresis with two targeted genes; Wiley Blackwell Publishing, Inc; Australian Journal Of Grape And Wine Research; 21; 1; 10-2014; 49-561322-7130CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/ajgw.12110info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ajgw.12110/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:55Zoai:ri.conicet.gov.ar:11336/36472instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:56.127CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Prevalence of Lactobacillus plantarum and Oenococcus oeni during spontaneous malolactic fermentation in Patagonian red wines revealed by polymerase chain reaction-denaturing gradient gel electrophoresis with two targeted genes
title Prevalence of Lactobacillus plantarum and Oenococcus oeni during spontaneous malolactic fermentation in Patagonian red wines revealed by polymerase chain reaction-denaturing gradient gel electrophoresis with two targeted genes
spellingShingle Prevalence of Lactobacillus plantarum and Oenococcus oeni during spontaneous malolactic fermentation in Patagonian red wines revealed by polymerase chain reaction-denaturing gradient gel electrophoresis with two targeted genes
Valdes la Hens, Danay
Lactobacillus Plantarum
Malolactic Fermentation
Oenococcus Oeni
Patagonian Red Wine
Pcr-Dgge
title_short Prevalence of Lactobacillus plantarum and Oenococcus oeni during spontaneous malolactic fermentation in Patagonian red wines revealed by polymerase chain reaction-denaturing gradient gel electrophoresis with two targeted genes
title_full Prevalence of Lactobacillus plantarum and Oenococcus oeni during spontaneous malolactic fermentation in Patagonian red wines revealed by polymerase chain reaction-denaturing gradient gel electrophoresis with two targeted genes
title_fullStr Prevalence of Lactobacillus plantarum and Oenococcus oeni during spontaneous malolactic fermentation in Patagonian red wines revealed by polymerase chain reaction-denaturing gradient gel electrophoresis with two targeted genes
title_full_unstemmed Prevalence of Lactobacillus plantarum and Oenococcus oeni during spontaneous malolactic fermentation in Patagonian red wines revealed by polymerase chain reaction-denaturing gradient gel electrophoresis with two targeted genes
title_sort Prevalence of Lactobacillus plantarum and Oenococcus oeni during spontaneous malolactic fermentation in Patagonian red wines revealed by polymerase chain reaction-denaturing gradient gel electrophoresis with two targeted genes
dc.creator.none.fl_str_mv Valdes la Hens, Danay
Bravo Ferrada, Barbara Mercedes
Delfederico, Lucrecia
Caballero, Adriana Catalina
Semorile, Liliana Carmen
author Valdes la Hens, Danay
author_facet Valdes la Hens, Danay
Bravo Ferrada, Barbara Mercedes
Delfederico, Lucrecia
Caballero, Adriana Catalina
Semorile, Liliana Carmen
author_role author
author2 Bravo Ferrada, Barbara Mercedes
Delfederico, Lucrecia
Caballero, Adriana Catalina
Semorile, Liliana Carmen
author2_role author
author
author
author
dc.subject.none.fl_str_mv Lactobacillus Plantarum
Malolactic Fermentation
Oenococcus Oeni
Patagonian Red Wine
Pcr-Dgge
topic Lactobacillus Plantarum
Malolactic Fermentation
Oenococcus Oeni
Patagonian Red Wine
Pcr-Dgge
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Background and Aims:Information regarding the composition and dynamics of microbial communities through-out winemaking is always valuable to control the process and contributes to improving the quality of wine. The aimsof this work were to analyse the diversity of lactic acid bacteria (LAB) species present during the spontaneousmalolactic fermentation (MLF) of Patagonian red wines, and to isolate and identify native LAB species.Methods and Results:Samples of Merlot and Pinot Noir wines were collected during three vintages at onecommercial cellar. Through polymerase chain reaction (PCR)-rpoB/denaturing gradient gel electrophoresis (DGGE),it was possible to identify 15 LAB species and through PCR-16S rRNAV3 region/DGGE, 11 LAB species, duringspontaneous MLF of these wines, mostly,Oenococcus oeniandLactobacillus plantarum. These two species were alsolargely recovered by culture and analysed by the random amplified polymorphic DNA–PCR technique. Isolates ofthese two LAB species exhibited considerable genotypic heterogeneity.Conclusions:The prevalence ofO. oeniandLb. plantarumin all samples suggests that both species are involved inleading the spontaneous MLF of these Patagonian wines. The analysis of the LAB microbiota in wine by PCR-DGGEusing two gene regions enabled addition to the number of species that can usually be detected during MLF.Significance of the Study:The study offered a more complete knowledge of the LAB community present in winesin a cellar in the Argentine Patagonia during the spontaneous MLF. Our findings are particularly relevant, becausethey contribute to the possibility of designing an MLF starter composed of native strains ofLb. plantarumandO. oeni
Fil: Valdes la Hens, Danay. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
Fil: Bravo Ferrada, Barbara Mercedes. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
Fil: Delfederico, Lucrecia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
Fil: Caballero, Adriana Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. IDEPA - Subsede San Antonio Oeste; Argentina. Universidad Nacional del Comahue; Argentina
Fil: Semorile, Liliana Carmen. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
description Background and Aims:Information regarding the composition and dynamics of microbial communities through-out winemaking is always valuable to control the process and contributes to improving the quality of wine. The aimsof this work were to analyse the diversity of lactic acid bacteria (LAB) species present during the spontaneousmalolactic fermentation (MLF) of Patagonian red wines, and to isolate and identify native LAB species.Methods and Results:Samples of Merlot and Pinot Noir wines were collected during three vintages at onecommercial cellar. Through polymerase chain reaction (PCR)-rpoB/denaturing gradient gel electrophoresis (DGGE),it was possible to identify 15 LAB species and through PCR-16S rRNAV3 region/DGGE, 11 LAB species, duringspontaneous MLF of these wines, mostly,Oenococcus oeniandLactobacillus plantarum. These two species were alsolargely recovered by culture and analysed by the random amplified polymorphic DNA–PCR technique. Isolates ofthese two LAB species exhibited considerable genotypic heterogeneity.Conclusions:The prevalence ofO. oeniandLb. plantarumin all samples suggests that both species are involved inleading the spontaneous MLF of these Patagonian wines. The analysis of the LAB microbiota in wine by PCR-DGGEusing two gene regions enabled addition to the number of species that can usually be detected during MLF.Significance of the Study:The study offered a more complete knowledge of the LAB community present in winesin a cellar in the Argentine Patagonia during the spontaneous MLF. Our findings are particularly relevant, becausethey contribute to the possibility of designing an MLF starter composed of native strains ofLb. plantarumandO. oeni
publishDate 2014
dc.date.none.fl_str_mv 2014-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36472
Valdes la Hens, Danay; Bravo Ferrada, Barbara Mercedes; Delfederico, Lucrecia; Caballero, Adriana Catalina; Semorile, Liliana Carmen; Prevalence of Lactobacillus plantarum and Oenococcus oeni during spontaneous malolactic fermentation in Patagonian red wines revealed by polymerase chain reaction-denaturing gradient gel electrophoresis with two targeted genes; Wiley Blackwell Publishing, Inc; Australian Journal Of Grape And Wine Research; 21; 1; 10-2014; 49-56
1322-7130
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36472
identifier_str_mv Valdes la Hens, Danay; Bravo Ferrada, Barbara Mercedes; Delfederico, Lucrecia; Caballero, Adriana Catalina; Semorile, Liliana Carmen; Prevalence of Lactobacillus plantarum and Oenococcus oeni during spontaneous malolactic fermentation in Patagonian red wines revealed by polymerase chain reaction-denaturing gradient gel electrophoresis with two targeted genes; Wiley Blackwell Publishing, Inc; Australian Journal Of Grape And Wine Research; 21; 1; 10-2014; 49-56
1322-7130
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/ajgw.12110
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/ajgw.12110/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270099745013760
score 13.13397

Publicaciones similares