Effect of melatonin on prednisolone eye disposition In cats

Autores
del Sole, Maria Jose; Schaiquevich, Paula Susana; Aba, Marcelo Alfredo; Lanusse, Carlos Edmundo; Moreno Torrejon, Laura
Año de publicación
2012
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Purpose: To characterize prednisolone (PRED) ocular and systemic pharmacokinetics in cats after oral administration and to study the effect of concomitant administration of melatonin (MEL) on PRED disposition. Methods: Six (6) castrated young physically and ophthalmologically healthy male European Short Hair cats were orally administrated with a single dose (10 mg) of PRED or a single dose of PRED (10 mg) and MEL (3 mg) in tablet. In anesthetized cats 2 mL of blood and 450 µL of aqueous humor (AH) were obtained from preplaced cephalic antebrachial intravenous catheters and from one eye by direct puncture, respectively, at: 0.25, 0.5, 1, 1.5, 2, 3, 4 and 5 h after administration. Plasma and AH samples were assayed for PRED by HPLC. A two-compartment model was used to simultaneously fit PRED plasma and aqueous concentration vs. time data using ADAPT 5. The estimated pharmacokinetic (PK) parameters included the absorption rate constant (ka), elimination rate constant from the central compartment (kc), intercompartment rate constants between plasma and aqueous humor (kpa), apparent volume of distribution of the central compartment (Vc/F). PK parameters were compared between groups (PRED vs. PRED+MEL) by means of Wilcoxon matched pairs test (p<0.05). Results: The model adequately fitted the data and the estimated median (interval) PK parameters are shown in Table 1. No significant difference was observed in the PK parameters when comparing between groups of treatments (p>0.05). Conclusion: These results indicated that MEL does not modify PRED systemic or ocular disposition in cats when both are administrated simultaneously. A possible synergic pharmacological effect may be account for different mechanisms of action, but not for pharmacokinetic synergism and will be further studied.
Fil: del Sole, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Endocrinología; Argentina
Fil: Schaiquevich, Paula Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan". Unidad de Farmacocinética Clínica. Laboratorio Farmacia; Argentina
Fil: Aba, Marcelo Alfredo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Endocrinología; Argentina
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Moreno Torrejon, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Association for Research in Vision and Ophthalmology Annual Meeting
Fort Laudardale
Estados Unidos
Association for Research in Vision and Ophthalmology
Materia
CORTICOSTEROIDS
MELATONIN
ANTIOXIDANTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/243393
Acceder
id CONICETDig_d163f8a8ce048c43ae748efb3062b378
oai_identifier_str oai:ri.conicet.gov.ar:11336/243393
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of melatonin on prednisolone eye disposition In catsdel Sole, Maria JoseSchaiquevich, Paula SusanaAba, Marcelo AlfredoLanusse, Carlos EdmundoMoreno Torrejon, LauraCORTICOSTEROIDSMELATONINANTIOXIDANTShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Purpose: To characterize prednisolone (PRED) ocular and systemic pharmacokinetics in cats after oral administration and to study the effect of concomitant administration of melatonin (MEL) on PRED disposition. Methods: Six (6) castrated young physically and ophthalmologically healthy male European Short Hair cats were orally administrated with a single dose (10 mg) of PRED or a single dose of PRED (10 mg) and MEL (3 mg) in tablet. In anesthetized cats 2 mL of blood and 450 µL of aqueous humor (AH) were obtained from preplaced cephalic antebrachial intravenous catheters and from one eye by direct puncture, respectively, at: 0.25, 0.5, 1, 1.5, 2, 3, 4 and 5 h after administration. Plasma and AH samples were assayed for PRED by HPLC. A two-compartment model was used to simultaneously fit PRED plasma and aqueous concentration vs. time data using ADAPT 5. The estimated pharmacokinetic (PK) parameters included the absorption rate constant (ka), elimination rate constant from the central compartment (kc), intercompartment rate constants between plasma and aqueous humor (kpa), apparent volume of distribution of the central compartment (Vc/F). PK parameters were compared between groups (PRED vs. PRED+MEL) by means of Wilcoxon matched pairs test (p<0.05). Results: The model adequately fitted the data and the estimated median (interval) PK parameters are shown in Table 1. No significant difference was observed in the PK parameters when comparing between groups of treatments (p>0.05). Conclusion: These results indicated that MEL does not modify PRED systemic or ocular disposition in cats when both are administrated simultaneously. A possible synergic pharmacological effect may be account for different mechanisms of action, but not for pharmacokinetic synergism and will be further studied.Fil: del Sole, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Endocrinología; ArgentinaFil: Schaiquevich, Paula Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan". Unidad de Farmacocinética Clínica. Laboratorio Farmacia; ArgentinaFil: Aba, Marcelo Alfredo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Endocrinología; ArgentinaFil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaFil: Moreno Torrejon, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaAssociation for Research in Vision and Ophthalmology Annual MeetingFort LaudardaleEstados UnidosAssociation for Research in Vision and OphthalmologyAssociation for Research in Vision and Ophthalmology2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/243393Effect of melatonin on prednisolone eye disposition In cats; Association for Research in Vision and Ophthalmology Annual Meeting; Fort Laudardale; Estados Unidos; 2012; 1-20146-0404CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://iovs.arvojournals.org/article.aspx?articleid=2349280&resultClick=1Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:29Zoai:ri.conicet.gov.ar:11336/243393instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:30.026CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of melatonin on prednisolone eye disposition In cats
title Effect of melatonin on prednisolone eye disposition In cats
spellingShingle Effect of melatonin on prednisolone eye disposition In cats
del Sole, Maria Jose
CORTICOSTEROIDS
MELATONIN
ANTIOXIDANTS
title_short Effect of melatonin on prednisolone eye disposition In cats
title_full Effect of melatonin on prednisolone eye disposition In cats
title_fullStr Effect of melatonin on prednisolone eye disposition In cats
title_full_unstemmed Effect of melatonin on prednisolone eye disposition In cats
title_sort Effect of melatonin on prednisolone eye disposition In cats
dc.creator.none.fl_str_mv del Sole, Maria Jose
Schaiquevich, Paula Susana
Aba, Marcelo Alfredo
Lanusse, Carlos Edmundo
Moreno Torrejon, Laura
author del Sole, Maria Jose
author_facet del Sole, Maria Jose
Schaiquevich, Paula Susana
Aba, Marcelo Alfredo
Lanusse, Carlos Edmundo
Moreno Torrejon, Laura
author_role author
author2 Schaiquevich, Paula Susana
Aba, Marcelo Alfredo
Lanusse, Carlos Edmundo
Moreno Torrejon, Laura
author2_role author
author
author
author
dc.subject.none.fl_str_mv CORTICOSTEROIDS
MELATONIN
ANTIOXIDANTS
topic CORTICOSTEROIDS
MELATONIN
ANTIOXIDANTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Purpose: To characterize prednisolone (PRED) ocular and systemic pharmacokinetics in cats after oral administration and to study the effect of concomitant administration of melatonin (MEL) on PRED disposition. Methods: Six (6) castrated young physically and ophthalmologically healthy male European Short Hair cats were orally administrated with a single dose (10 mg) of PRED or a single dose of PRED (10 mg) and MEL (3 mg) in tablet. In anesthetized cats 2 mL of blood and 450 µL of aqueous humor (AH) were obtained from preplaced cephalic antebrachial intravenous catheters and from one eye by direct puncture, respectively, at: 0.25, 0.5, 1, 1.5, 2, 3, 4 and 5 h after administration. Plasma and AH samples were assayed for PRED by HPLC. A two-compartment model was used to simultaneously fit PRED plasma and aqueous concentration vs. time data using ADAPT 5. The estimated pharmacokinetic (PK) parameters included the absorption rate constant (ka), elimination rate constant from the central compartment (kc), intercompartment rate constants between plasma and aqueous humor (kpa), apparent volume of distribution of the central compartment (Vc/F). PK parameters were compared between groups (PRED vs. PRED+MEL) by means of Wilcoxon matched pairs test (p<0.05). Results: The model adequately fitted the data and the estimated median (interval) PK parameters are shown in Table 1. No significant difference was observed in the PK parameters when comparing between groups of treatments (p>0.05). Conclusion: These results indicated that MEL does not modify PRED systemic or ocular disposition in cats when both are administrated simultaneously. A possible synergic pharmacological effect may be account for different mechanisms of action, but not for pharmacokinetic synergism and will be further studied.
Fil: del Sole, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Endocrinología; Argentina
Fil: Schaiquevich, Paula Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan". Unidad de Farmacocinética Clínica. Laboratorio Farmacia; Argentina
Fil: Aba, Marcelo Alfredo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Endocrinología; Argentina
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Moreno Torrejon, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Association for Research in Vision and Ophthalmology Annual Meeting
Fort Laudardale
Estados Unidos
Association for Research in Vision and Ophthalmology
description Purpose: To characterize prednisolone (PRED) ocular and systemic pharmacokinetics in cats after oral administration and to study the effect of concomitant administration of melatonin (MEL) on PRED disposition. Methods: Six (6) castrated young physically and ophthalmologically healthy male European Short Hair cats were orally administrated with a single dose (10 mg) of PRED or a single dose of PRED (10 mg) and MEL (3 mg) in tablet. In anesthetized cats 2 mL of blood and 450 µL of aqueous humor (AH) were obtained from preplaced cephalic antebrachial intravenous catheters and from one eye by direct puncture, respectively, at: 0.25, 0.5, 1, 1.5, 2, 3, 4 and 5 h after administration. Plasma and AH samples were assayed for PRED by HPLC. A two-compartment model was used to simultaneously fit PRED plasma and aqueous concentration vs. time data using ADAPT 5. The estimated pharmacokinetic (PK) parameters included the absorption rate constant (ka), elimination rate constant from the central compartment (kc), intercompartment rate constants between plasma and aqueous humor (kpa), apparent volume of distribution of the central compartment (Vc/F). PK parameters were compared between groups (PRED vs. PRED+MEL) by means of Wilcoxon matched pairs test (p<0.05). Results: The model adequately fitted the data and the estimated median (interval) PK parameters are shown in Table 1. No significant difference was observed in the PK parameters when comparing between groups of treatments (p>0.05). Conclusion: These results indicated that MEL does not modify PRED systemic or ocular disposition in cats when both are administrated simultaneously. A possible synergic pharmacological effect may be account for different mechanisms of action, but not for pharmacokinetic synergism and will be further studied.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243393
Effect of melatonin on prednisolone eye disposition In cats; Association for Research in Vision and Ophthalmology Annual Meeting; Fort Laudardale; Estados Unidos; 2012; 1-2
0146-0404
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243393
identifier_str_mv Effect of melatonin on prednisolone eye disposition In cats; Association for Research in Vision and Ophthalmology Annual Meeting; Fort Laudardale; Estados Unidos; 2012; 1-2
0146-0404
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://iovs.arvojournals.org/article.aspx?articleid=2349280&resultClick=1
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/vnd.openxmlformats-officedocument.wordprocessingml.document
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Association for Research in Vision and Ophthalmology
publisher.none.fl_str_mv Association for Research in Vision and Ophthalmology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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