Plasma and Ocular Prednisolone Disposition after Oral Treatment in Cats

Autores
Lanusse, Carlos Edmundo; Schaiquevich, Paula Susana; Aba, Marcelo Alfredo; Moreno Torrejon, Laura; del Sole, Maria Jose
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To evaluate the plasma and aqueous humor disposition of prednisolone after oral administration in cats. Methods: Six cats were administered with a single oral dose of prednisolone (10 mg). Blood and aqueous humor samples were serially collected after drug administration. Prednisolone concentrations in plasma and aqueous humor were measured at 0.25, 0.5, 1.0, 1.5, 2.0, 3.0, 4.0 and 5.0 h after administration by a high-performance liquid chromatographic analytical method developed and validated for this purpose. Results: Mean±standard error (SE) of maximum plasma prednisolone concentration (300.8±67.3 ng/mL) was reached at 1 h post-administration. Prednisolone distributed to the aqueous humor reaching a mean peak concentration of 100.9±25.5 ng/mL at 1.25 h post-administration. The mean ± SE systemic and aqueous humor exposure (AUC) was 553.3±120.0 ng*h/mL and 378.8±64.9 ng*h/mL, respectively. A high AUCaqueous humor/AUCplasma ratio was observed (0.68±0.13). The mean half life time of elimination in plasma and aqueous humor was 0.87±0.16 h and 2.25±0.44 h, respectively. Clinical Significance: The observed high ratio between aqueous humor and plasma concentration indicates that extensive penetration of prednisolone to the anterior segment of the eye may occur. This is the first step that contributes to optimize the pharmacological therapeutics for the clinical treatment of uveitis.
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Schaiquevich, Paula Susana. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatria "juan P.garrahan". Unidad de Farmacocinetica Clinica. Laboratorio Farmacia; Argentina
Fil: Aba, Marcelo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Moreno Torrejon, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: del Sole, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Materia
Oral prednisolone
Cat
Plasma
Aqueous humor
Pharmacokinetic
HPLC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4698
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4698
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Plasma and Ocular Prednisolone Disposition after Oral Treatment in CatsLanusse, Carlos EdmundoSchaiquevich, Paula SusanaAba, Marcelo AlfredoMoreno Torrejon, Lauradel Sole, Maria JoseOral prednisoloneCatPlasmaAqueous humorPharmacokineticHPLChttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Objective: To evaluate the plasma and aqueous humor disposition of prednisolone after oral administration in cats. Methods: Six cats were administered with a single oral dose of prednisolone (10 mg). Blood and aqueous humor samples were serially collected after drug administration. Prednisolone concentrations in plasma and aqueous humor were measured at 0.25, 0.5, 1.0, 1.5, 2.0, 3.0, 4.0 and 5.0 h after administration by a high-performance liquid chromatographic analytical method developed and validated for this purpose. Results: Mean±standard error (SE) of maximum plasma prednisolone concentration (300.8±67.3 ng/mL) was reached at 1 h post-administration. Prednisolone distributed to the aqueous humor reaching a mean peak concentration of 100.9±25.5 ng/mL at 1.25 h post-administration. The mean ± SE systemic and aqueous humor exposure (AUC) was 553.3±120.0 ng*h/mL and 378.8±64.9 ng*h/mL, respectively. A high AUCaqueous humor/AUCplasma ratio was observed (0.68±0.13). The mean half life time of elimination in plasma and aqueous humor was 0.87±0.16 h and 2.25±0.44 h, respectively. Clinical Significance: The observed high ratio between aqueous humor and plasma concentration indicates that extensive penetration of prednisolone to the anterior segment of the eye may occur. This is the first step that contributes to optimize the pharmacological therapeutics for the clinical treatment of uveitis.Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaFil: Schaiquevich, Paula Susana. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatria "juan P.garrahan". Unidad de Farmacocinetica Clinica. Laboratorio Farmacia; ArgentinaFil: Aba, Marcelo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaFil: Moreno Torrejon, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaFil: del Sole, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaHindawi Publishing Corporation2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4698Lanusse, Carlos Edmundo; Schaiquevich, Paula Susana; Aba, Marcelo Alfredo; Moreno Torrejon, Laura; del Sole, Maria Jose; Plasma and Ocular Prednisolone Disposition after Oral Treatment in Cats; Hindawi Publishing Corporation; BioMed Research International; 9-2013; 209439-2094392314-6133enginfo:eu-repo/semantics/altIdentifier/doi/10.1155/2013/209439info:eu-repo/semantics/altIdentifier/url/http://www.hindawi.com/journals/bmri/2013/209439/info:eu-repo/semantics/altIdentifier/issn/2314-6133info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:10Zoai:ri.conicet.gov.ar:11336/4698instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:10.432CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Plasma and Ocular Prednisolone Disposition after Oral Treatment in Cats
title Plasma and Ocular Prednisolone Disposition after Oral Treatment in Cats
spellingShingle Plasma and Ocular Prednisolone Disposition after Oral Treatment in Cats
Lanusse, Carlos Edmundo
Oral prednisolone
Cat
Plasma
Aqueous humor
Pharmacokinetic
HPLC
title_short Plasma and Ocular Prednisolone Disposition after Oral Treatment in Cats
title_full Plasma and Ocular Prednisolone Disposition after Oral Treatment in Cats
title_fullStr Plasma and Ocular Prednisolone Disposition after Oral Treatment in Cats
title_full_unstemmed Plasma and Ocular Prednisolone Disposition after Oral Treatment in Cats
title_sort Plasma and Ocular Prednisolone Disposition after Oral Treatment in Cats
dc.creator.none.fl_str_mv Lanusse, Carlos Edmundo
Schaiquevich, Paula Susana
Aba, Marcelo Alfredo
Moreno Torrejon, Laura
del Sole, Maria Jose
author Lanusse, Carlos Edmundo
author_facet Lanusse, Carlos Edmundo
Schaiquevich, Paula Susana
Aba, Marcelo Alfredo
Moreno Torrejon, Laura
del Sole, Maria Jose
author_role author
author2 Schaiquevich, Paula Susana
Aba, Marcelo Alfredo
Moreno Torrejon, Laura
del Sole, Maria Jose
author2_role author
author
author
author
dc.subject.none.fl_str_mv Oral prednisolone
Cat
Plasma
Aqueous humor
Pharmacokinetic
HPLC
topic Oral prednisolone
Cat
Plasma
Aqueous humor
Pharmacokinetic
HPLC
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Objective: To evaluate the plasma and aqueous humor disposition of prednisolone after oral administration in cats. Methods: Six cats were administered with a single oral dose of prednisolone (10 mg). Blood and aqueous humor samples were serially collected after drug administration. Prednisolone concentrations in plasma and aqueous humor were measured at 0.25, 0.5, 1.0, 1.5, 2.0, 3.0, 4.0 and 5.0 h after administration by a high-performance liquid chromatographic analytical method developed and validated for this purpose. Results: Mean±standard error (SE) of maximum plasma prednisolone concentration (300.8±67.3 ng/mL) was reached at 1 h post-administration. Prednisolone distributed to the aqueous humor reaching a mean peak concentration of 100.9±25.5 ng/mL at 1.25 h post-administration. The mean ± SE systemic and aqueous humor exposure (AUC) was 553.3±120.0 ng*h/mL and 378.8±64.9 ng*h/mL, respectively. A high AUCaqueous humor/AUCplasma ratio was observed (0.68±0.13). The mean half life time of elimination in plasma and aqueous humor was 0.87±0.16 h and 2.25±0.44 h, respectively. Clinical Significance: The observed high ratio between aqueous humor and plasma concentration indicates that extensive penetration of prednisolone to the anterior segment of the eye may occur. This is the first step that contributes to optimize the pharmacological therapeutics for the clinical treatment of uveitis.
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Schaiquevich, Paula Susana. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatria "juan P.garrahan". Unidad de Farmacocinetica Clinica. Laboratorio Farmacia; Argentina
Fil: Aba, Marcelo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Moreno Torrejon, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: del Sole, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
description Objective: To evaluate the plasma and aqueous humor disposition of prednisolone after oral administration in cats. Methods: Six cats were administered with a single oral dose of prednisolone (10 mg). Blood and aqueous humor samples were serially collected after drug administration. Prednisolone concentrations in plasma and aqueous humor were measured at 0.25, 0.5, 1.0, 1.5, 2.0, 3.0, 4.0 and 5.0 h after administration by a high-performance liquid chromatographic analytical method developed and validated for this purpose. Results: Mean±standard error (SE) of maximum plasma prednisolone concentration (300.8±67.3 ng/mL) was reached at 1 h post-administration. Prednisolone distributed to the aqueous humor reaching a mean peak concentration of 100.9±25.5 ng/mL at 1.25 h post-administration. The mean ± SE systemic and aqueous humor exposure (AUC) was 553.3±120.0 ng*h/mL and 378.8±64.9 ng*h/mL, respectively. A high AUCaqueous humor/AUCplasma ratio was observed (0.68±0.13). The mean half life time of elimination in plasma and aqueous humor was 0.87±0.16 h and 2.25±0.44 h, respectively. Clinical Significance: The observed high ratio between aqueous humor and plasma concentration indicates that extensive penetration of prednisolone to the anterior segment of the eye may occur. This is the first step that contributes to optimize the pharmacological therapeutics for the clinical treatment of uveitis.
publishDate 2013
dc.date.none.fl_str_mv 2013-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4698
Lanusse, Carlos Edmundo; Schaiquevich, Paula Susana; Aba, Marcelo Alfredo; Moreno Torrejon, Laura; del Sole, Maria Jose; Plasma and Ocular Prednisolone Disposition after Oral Treatment in Cats; Hindawi Publishing Corporation; BioMed Research International; 9-2013; 209439-209439
2314-6133
url http://hdl.handle.net/11336/4698
identifier_str_mv Lanusse, Carlos Edmundo; Schaiquevich, Paula Susana; Aba, Marcelo Alfredo; Moreno Torrejon, Laura; del Sole, Maria Jose; Plasma and Ocular Prednisolone Disposition after Oral Treatment in Cats; Hindawi Publishing Corporation; BioMed Research International; 9-2013; 209439-209439
2314-6133
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1155/2013/209439
info:eu-repo/semantics/altIdentifier/url/http://www.hindawi.com/journals/bmri/2013/209439/
info:eu-repo/semantics/altIdentifier/issn/2314-6133
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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