Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells
- Autores
- Pérez Pérez, Antonio; Maymo, Julieta Lorena; Gambino, Yésica Paola; Dueñas, José L.; Goberna, Raimundo; Varone, Cecilia Laura; Sánchez Margalet, Víctor
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Leptin was originally considered as an adipocyte-derived signaling molecule for the central control of metabolism. However, pleiotropic effects of leptin have been identified in reproduction and pregnancy, particularly in placenta, where it may work as an autocrine hormone, mediating angiogenesis, growth, and immunomodulation. Leptin receptor (LEPR, also known as Ob-R) shows sequence homology to members of the class I cytokine receptor (gp130) superfamily. In fact, leptin may function as a proinflammatory cytokine. We have previously found that leptin is a trophic and mitogenic factor for trophoblastic cells. In order to further investigate the mechanism by which leptin stimulates cell growth in JEG-3 cells and trophoblastic cells, we studied the phosphorylation state of different proteins of the initiation stage of translation and the total protein synthesis by [3H]leucine incorporation in JEG-3 cells. We have found that leptin dose-dependently stimulates the phosphorylation and activation of the translation initiation factor EIF4E as well as the phosphorylation of the EIF4E binding protein EIF4EBP1 (PHAS-I), which releases EIF4E to form active complexes. Moreover, leptin dose-dependently stimulates protein synthesis, and this effect can be partially prevented by blocking mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3 kinase (PIK3) pathways. In conclusion, leptin stimulates protein synthesis, at least in part activating the translation machinery, via the activation of MAPK and PIK3 pathways. © 2009 by the Society for the Study of Reproduction, Inc.
Fil: Pérez Pérez, Antonio. Universidad de Sevilla; España
Fil: Maymo, Julieta Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Gambino, Yésica Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Dueñas, José L.. Universidad de Sevilla; España
Fil: Goberna, Raimundo. Universidad de Sevilla; España
Fil: Varone, Cecilia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Sánchez Margalet, Víctor. Universidad de Sevilla; España - Materia
-
Leptin
Leptin Receptor
Mechanisms of Hormone Action
Placenta
Trophoblast - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/67270
Ver los metadatos del registro completo
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Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cellsPérez Pérez, AntonioMaymo, Julieta LorenaGambino, Yésica PaolaDueñas, José L.Goberna, RaimundoVarone, Cecilia LauraSánchez Margalet, VíctorLeptinLeptin ReceptorMechanisms of Hormone ActionPlacentaTrophoblasthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Leptin was originally considered as an adipocyte-derived signaling molecule for the central control of metabolism. However, pleiotropic effects of leptin have been identified in reproduction and pregnancy, particularly in placenta, where it may work as an autocrine hormone, mediating angiogenesis, growth, and immunomodulation. Leptin receptor (LEPR, also known as Ob-R) shows sequence homology to members of the class I cytokine receptor (gp130) superfamily. In fact, leptin may function as a proinflammatory cytokine. We have previously found that leptin is a trophic and mitogenic factor for trophoblastic cells. In order to further investigate the mechanism by which leptin stimulates cell growth in JEG-3 cells and trophoblastic cells, we studied the phosphorylation state of different proteins of the initiation stage of translation and the total protein synthesis by [3H]leucine incorporation in JEG-3 cells. We have found that leptin dose-dependently stimulates the phosphorylation and activation of the translation initiation factor EIF4E as well as the phosphorylation of the EIF4E binding protein EIF4EBP1 (PHAS-I), which releases EIF4E to form active complexes. Moreover, leptin dose-dependently stimulates protein synthesis, and this effect can be partially prevented by blocking mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3 kinase (PIK3) pathways. In conclusion, leptin stimulates protein synthesis, at least in part activating the translation machinery, via the activation of MAPK and PIK3 pathways. © 2009 by the Society for the Study of Reproduction, Inc.Fil: Pérez Pérez, Antonio. Universidad de Sevilla; EspañaFil: Maymo, Julieta Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Gambino, Yésica Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Dueñas, José L.. Universidad de Sevilla; EspañaFil: Goberna, Raimundo. Universidad de Sevilla; EspañaFil: Varone, Cecilia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Sánchez Margalet, Víctor. Universidad de Sevilla; EspañaSociety for the Study of Reproduction2009-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67270Pérez Pérez, Antonio; Maymo, Julieta Lorena; Gambino, Yésica Paola; Dueñas, José L.; Goberna, Raimundo; et al.; Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells; Society for the Study of Reproduction; Biology of Reproduction; 81; 5; 6-2009; 826-8320006-3363CONICET DigitalCONICETenginfo:eu-repo/semantics/reference/url/http://hdl.handle.net/11336/11683info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.109.076513info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/81/5/826/2557758info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:48Zoai:ri.conicet.gov.ar:11336/67270instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:48.62CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells |
title |
Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells |
spellingShingle |
Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells Pérez Pérez, Antonio Leptin Leptin Receptor Mechanisms of Hormone Action Placenta Trophoblast |
title_short |
Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells |
title_full |
Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells |
title_fullStr |
Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells |
title_full_unstemmed |
Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells |
title_sort |
Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells |
dc.creator.none.fl_str_mv |
Pérez Pérez, Antonio Maymo, Julieta Lorena Gambino, Yésica Paola Dueñas, José L. Goberna, Raimundo Varone, Cecilia Laura Sánchez Margalet, Víctor |
author |
Pérez Pérez, Antonio |
author_facet |
Pérez Pérez, Antonio Maymo, Julieta Lorena Gambino, Yésica Paola Dueñas, José L. Goberna, Raimundo Varone, Cecilia Laura Sánchez Margalet, Víctor |
author_role |
author |
author2 |
Maymo, Julieta Lorena Gambino, Yésica Paola Dueñas, José L. Goberna, Raimundo Varone, Cecilia Laura Sánchez Margalet, Víctor |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Leptin Leptin Receptor Mechanisms of Hormone Action Placenta Trophoblast |
topic |
Leptin Leptin Receptor Mechanisms of Hormone Action Placenta Trophoblast |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Leptin was originally considered as an adipocyte-derived signaling molecule for the central control of metabolism. However, pleiotropic effects of leptin have been identified in reproduction and pregnancy, particularly in placenta, where it may work as an autocrine hormone, mediating angiogenesis, growth, and immunomodulation. Leptin receptor (LEPR, also known as Ob-R) shows sequence homology to members of the class I cytokine receptor (gp130) superfamily. In fact, leptin may function as a proinflammatory cytokine. We have previously found that leptin is a trophic and mitogenic factor for trophoblastic cells. In order to further investigate the mechanism by which leptin stimulates cell growth in JEG-3 cells and trophoblastic cells, we studied the phosphorylation state of different proteins of the initiation stage of translation and the total protein synthesis by [3H]leucine incorporation in JEG-3 cells. We have found that leptin dose-dependently stimulates the phosphorylation and activation of the translation initiation factor EIF4E as well as the phosphorylation of the EIF4E binding protein EIF4EBP1 (PHAS-I), which releases EIF4E to form active complexes. Moreover, leptin dose-dependently stimulates protein synthesis, and this effect can be partially prevented by blocking mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3 kinase (PIK3) pathways. In conclusion, leptin stimulates protein synthesis, at least in part activating the translation machinery, via the activation of MAPK and PIK3 pathways. © 2009 by the Society for the Study of Reproduction, Inc. Fil: Pérez Pérez, Antonio. Universidad de Sevilla; España Fil: Maymo, Julieta Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina Fil: Gambino, Yésica Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina Fil: Dueñas, José L.. Universidad de Sevilla; España Fil: Goberna, Raimundo. Universidad de Sevilla; España Fil: Varone, Cecilia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina Fil: Sánchez Margalet, Víctor. Universidad de Sevilla; España |
description |
Leptin was originally considered as an adipocyte-derived signaling molecule for the central control of metabolism. However, pleiotropic effects of leptin have been identified in reproduction and pregnancy, particularly in placenta, where it may work as an autocrine hormone, mediating angiogenesis, growth, and immunomodulation. Leptin receptor (LEPR, also known as Ob-R) shows sequence homology to members of the class I cytokine receptor (gp130) superfamily. In fact, leptin may function as a proinflammatory cytokine. We have previously found that leptin is a trophic and mitogenic factor for trophoblastic cells. In order to further investigate the mechanism by which leptin stimulates cell growth in JEG-3 cells and trophoblastic cells, we studied the phosphorylation state of different proteins of the initiation stage of translation and the total protein synthesis by [3H]leucine incorporation in JEG-3 cells. We have found that leptin dose-dependently stimulates the phosphorylation and activation of the translation initiation factor EIF4E as well as the phosphorylation of the EIF4E binding protein EIF4EBP1 (PHAS-I), which releases EIF4E to form active complexes. Moreover, leptin dose-dependently stimulates protein synthesis, and this effect can be partially prevented by blocking mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3 kinase (PIK3) pathways. In conclusion, leptin stimulates protein synthesis, at least in part activating the translation machinery, via the activation of MAPK and PIK3 pathways. © 2009 by the Society for the Study of Reproduction, Inc. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/67270 Pérez Pérez, Antonio; Maymo, Julieta Lorena; Gambino, Yésica Paola; Dueñas, José L.; Goberna, Raimundo; et al.; Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells; Society for the Study of Reproduction; Biology of Reproduction; 81; 5; 6-2009; 826-832 0006-3363 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/67270 |
identifier_str_mv |
Pérez Pérez, Antonio; Maymo, Julieta Lorena; Gambino, Yésica Paola; Dueñas, José L.; Goberna, Raimundo; et al.; Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells; Society for the Study of Reproduction; Biology of Reproduction; 81; 5; 6-2009; 826-832 0006-3363 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/reference/url/http://hdl.handle.net/11336/11683 info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.109.076513 info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/81/5/826/2557758 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Society for the Study of Reproduction |
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Society for the Study of Reproduction |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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