Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells

Autores
Pérez Pérez, Antonio; Maymo, Julieta Lorena; Gambino, Yésica Paola; Dueñas, José L.; Goberna, Raimundo; Varone, Cecilia Laura; Sánchez Margalet, Víctor
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leptin was originally considered as an adipocyte-derived signaling molecule for the central control of metabolism. However, pleiotropic effects of leptin have been identified in reproduction and pregnancy, particularly in placenta, where it may work as an autocrine hormone, mediating angiogenesis, growth, and immunomodulation. Leptin receptor (LEPR, also known as Ob-R) shows sequence homology to members of the class I cytokine receptor (gp130) superfamily. In fact, leptin may function as a proinflammatory cytokine. We have previously found that leptin is a trophic and mitogenic factor for trophoblastic cells. In order to further investigate the mechanism by which leptin stimulates cell growth in JEG-3 cells and trophoblastic cells, we studied the phosphorylation state of different proteins of the initiation stage of translation and the total protein synthesis by [3H]leucine incorporation in JEG-3 cells. We have found that leptin dose-dependently stimulates the phosphorylation and activation of the translation initiation factor EIF4E as well as the phosphorylation of the EIF4E binding protein EIF4EBP1 (PHAS-I), which releases EIF4E to form active complexes. Moreover, leptin dose-dependently stimulates protein synthesis, and this effect can be partially prevented by blocking mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3 kinase (PIK3) pathways. In conclusion, leptin stimulates protein synthesis, at least in part activating the translation machinery, via the activation of MAPK and PIK3 pathways. © 2009 by the Society for the Study of Reproduction, Inc.
Fil: Pérez Pérez, Antonio. Universidad de Sevilla; España
Fil: Maymo, Julieta Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Gambino, Yésica Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Dueñas, José L.. Universidad de Sevilla; España
Fil: Goberna, Raimundo. Universidad de Sevilla; España
Fil: Varone, Cecilia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Sánchez Margalet, Víctor. Universidad de Sevilla; España
Materia
Leptin
Leptin Receptor
Mechanisms of Hormone Action
Placenta
Trophoblast
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67270
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/67270
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cellsPérez Pérez, AntonioMaymo, Julieta LorenaGambino, Yésica PaolaDueñas, José L.Goberna, RaimundoVarone, Cecilia LauraSánchez Margalet, VíctorLeptinLeptin ReceptorMechanisms of Hormone ActionPlacentaTrophoblasthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Leptin was originally considered as an adipocyte-derived signaling molecule for the central control of metabolism. However, pleiotropic effects of leptin have been identified in reproduction and pregnancy, particularly in placenta, where it may work as an autocrine hormone, mediating angiogenesis, growth, and immunomodulation. Leptin receptor (LEPR, also known as Ob-R) shows sequence homology to members of the class I cytokine receptor (gp130) superfamily. In fact, leptin may function as a proinflammatory cytokine. We have previously found that leptin is a trophic and mitogenic factor for trophoblastic cells. In order to further investigate the mechanism by which leptin stimulates cell growth in JEG-3 cells and trophoblastic cells, we studied the phosphorylation state of different proteins of the initiation stage of translation and the total protein synthesis by [3H]leucine incorporation in JEG-3 cells. We have found that leptin dose-dependently stimulates the phosphorylation and activation of the translation initiation factor EIF4E as well as the phosphorylation of the EIF4E binding protein EIF4EBP1 (PHAS-I), which releases EIF4E to form active complexes. Moreover, leptin dose-dependently stimulates protein synthesis, and this effect can be partially prevented by blocking mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3 kinase (PIK3) pathways. In conclusion, leptin stimulates protein synthesis, at least in part activating the translation machinery, via the activation of MAPK and PIK3 pathways. © 2009 by the Society for the Study of Reproduction, Inc.Fil: Pérez Pérez, Antonio. Universidad de Sevilla; EspañaFil: Maymo, Julieta Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Gambino, Yésica Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Dueñas, José L.. Universidad de Sevilla; EspañaFil: Goberna, Raimundo. Universidad de Sevilla; EspañaFil: Varone, Cecilia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Sánchez Margalet, Víctor. Universidad de Sevilla; EspañaSociety for the Study of Reproduction2009-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67270Pérez Pérez, Antonio; Maymo, Julieta Lorena; Gambino, Yésica Paola; Dueñas, José L.; Goberna, Raimundo; et al.; Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells; Society for the Study of Reproduction; Biology of Reproduction; 81; 5; 6-2009; 826-8320006-3363CONICET DigitalCONICETenginfo:eu-repo/semantics/reference/url/http://hdl.handle.net/11336/11683info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.109.076513info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/81/5/826/2557758info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:46:52Zoai:ri.conicet.gov.ar:11336/67270instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:46:52.444CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells
title Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells
spellingShingle Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells
Pérez Pérez, Antonio
Leptin
Leptin Receptor
Mechanisms of Hormone Action
Placenta
Trophoblast
title_short Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells
title_full Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells
title_fullStr Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells
title_full_unstemmed Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells
title_sort Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells
dc.creator.none.fl_str_mv Pérez Pérez, Antonio
Maymo, Julieta Lorena
Gambino, Yésica Paola
Dueñas, José L.
Goberna, Raimundo
Varone, Cecilia Laura
Sánchez Margalet, Víctor
author Pérez Pérez, Antonio
author_facet Pérez Pérez, Antonio
Maymo, Julieta Lorena
Gambino, Yésica Paola
Dueñas, José L.
Goberna, Raimundo
Varone, Cecilia Laura
Sánchez Margalet, Víctor
author_role author
author2 Maymo, Julieta Lorena
Gambino, Yésica Paola
Dueñas, José L.
Goberna, Raimundo
Varone, Cecilia Laura
Sánchez Margalet, Víctor
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Leptin
Leptin Receptor
Mechanisms of Hormone Action
Placenta
Trophoblast
topic Leptin
Leptin Receptor
Mechanisms of Hormone Action
Placenta
Trophoblast
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Leptin was originally considered as an adipocyte-derived signaling molecule for the central control of metabolism. However, pleiotropic effects of leptin have been identified in reproduction and pregnancy, particularly in placenta, where it may work as an autocrine hormone, mediating angiogenesis, growth, and immunomodulation. Leptin receptor (LEPR, also known as Ob-R) shows sequence homology to members of the class I cytokine receptor (gp130) superfamily. In fact, leptin may function as a proinflammatory cytokine. We have previously found that leptin is a trophic and mitogenic factor for trophoblastic cells. In order to further investigate the mechanism by which leptin stimulates cell growth in JEG-3 cells and trophoblastic cells, we studied the phosphorylation state of different proteins of the initiation stage of translation and the total protein synthesis by [3H]leucine incorporation in JEG-3 cells. We have found that leptin dose-dependently stimulates the phosphorylation and activation of the translation initiation factor EIF4E as well as the phosphorylation of the EIF4E binding protein EIF4EBP1 (PHAS-I), which releases EIF4E to form active complexes. Moreover, leptin dose-dependently stimulates protein synthesis, and this effect can be partially prevented by blocking mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3 kinase (PIK3) pathways. In conclusion, leptin stimulates protein synthesis, at least in part activating the translation machinery, via the activation of MAPK and PIK3 pathways. © 2009 by the Society for the Study of Reproduction, Inc.
Fil: Pérez Pérez, Antonio. Universidad de Sevilla; España
Fil: Maymo, Julieta Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Gambino, Yésica Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Dueñas, José L.. Universidad de Sevilla; España
Fil: Goberna, Raimundo. Universidad de Sevilla; España
Fil: Varone, Cecilia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Sánchez Margalet, Víctor. Universidad de Sevilla; España
description Leptin was originally considered as an adipocyte-derived signaling molecule for the central control of metabolism. However, pleiotropic effects of leptin have been identified in reproduction and pregnancy, particularly in placenta, where it may work as an autocrine hormone, mediating angiogenesis, growth, and immunomodulation. Leptin receptor (LEPR, also known as Ob-R) shows sequence homology to members of the class I cytokine receptor (gp130) superfamily. In fact, leptin may function as a proinflammatory cytokine. We have previously found that leptin is a trophic and mitogenic factor for trophoblastic cells. In order to further investigate the mechanism by which leptin stimulates cell growth in JEG-3 cells and trophoblastic cells, we studied the phosphorylation state of different proteins of the initiation stage of translation and the total protein synthesis by [3H]leucine incorporation in JEG-3 cells. We have found that leptin dose-dependently stimulates the phosphorylation and activation of the translation initiation factor EIF4E as well as the phosphorylation of the EIF4E binding protein EIF4EBP1 (PHAS-I), which releases EIF4E to form active complexes. Moreover, leptin dose-dependently stimulates protein synthesis, and this effect can be partially prevented by blocking mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3 kinase (PIK3) pathways. In conclusion, leptin stimulates protein synthesis, at least in part activating the translation machinery, via the activation of MAPK and PIK3 pathways. © 2009 by the Society for the Study of Reproduction, Inc.
publishDate 2009
dc.date.none.fl_str_mv 2009-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67270
Pérez Pérez, Antonio; Maymo, Julieta Lorena; Gambino, Yésica Paola; Dueñas, José L.; Goberna, Raimundo; et al.; Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells; Society for the Study of Reproduction; Biology of Reproduction; 81; 5; 6-2009; 826-832
0006-3363
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67270
identifier_str_mv Pérez Pérez, Antonio; Maymo, Julieta Lorena; Gambino, Yésica Paola; Dueñas, José L.; Goberna, Raimundo; et al.; Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells; Society for the Study of Reproduction; Biology of Reproduction; 81; 5; 6-2009; 826-832
0006-3363
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/reference/url/http://hdl.handle.net/11336/11683
info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.109.076513
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/81/5/826/2557758
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for the Study of Reproduction
publisher.none.fl_str_mv Society for the Study of Reproduction
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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