A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines

Autores
Peris, Leticia; Bisbal, Mariano; Martinez Hernandez, José; Saoudi, Yasmina; Jonckheere, Julie; Rolland, Marta; Sebastien, Muriel; Brocard, Jacques; Denarier, Eric; Bosc, Christopher; Guerin, Christophe; Gory Fauré, Sylvie; Deloulme, Jean Christophe; Lanté, Fabien; Arnal, Isabelle; Buisson, Alain; Goldberg, Yves; Blanchoin, Laurent; Delphin, Christian; Andrieux, Annie
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Emerging evidence indicates that microtubule-associated proteins (MAPs) are implicated in synaptic function; in particular, mice deficient for MAP6 exhibit striking deficits in plasticity and cognition. How MAP6 connects to plasticity mechanisms is unclear. Here, we address the possible role of this protein in dendritic spines. We find that in MAP6-deficient cortical and hippocampal neurons, maintenance of mature spines is impaired, and can be restored by expressing a stretch of the MAP6 sequence called Mc modules. Mc modules directly bind actin filaments and mediate activity-dependent stabilisation of F-actin in dendritic spines, a key event of synaptic plasticity. In vitro, Mc modules enhance actin filament nucleation and promote the formation of stable, highly ordered filament bundles. Activity-induced phosphorylation of MAP6 likely controls its transfer to the spine cytoskeleton. These results provide a molecular explanation for the role of MAP6 in cognition, enlightening the connection between cytoskeletal dysfunction, synaptic impairment and neuropsychiatric illnesses.
Fil: Peris, Leticia. Universite Grenoble Alpes; Francia
Fil: Bisbal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universite Grenoble Alpes; Francia
Fil: Martinez Hernandez, José. Universidad del País Vasco; España. Universite Grenoble Alpes; Francia
Fil: Saoudi, Yasmina. Universite Grenoble Alpes; Francia
Fil: Jonckheere, Julie. Universite Grenoble Alpes; Francia
Fil: Rolland, Marta. Universite Grenoble Alpes; Francia
Fil: Sebastien, Muriel. Universite Grenoble Alpes; Francia
Fil: Brocard, Jacques. Universite Grenoble Alpes; Francia
Fil: Denarier, Eric. Universite Grenoble Alpes; Francia
Fil: Bosc, Christopher. Universite Grenoble Alpes; Francia
Fil: Guerin, Christophe. Universite Grenoble Alpes; Francia. Inserm; Francia
Fil: Gory Fauré, Sylvie. Universite Grenoble Alpes; Francia
Fil: Deloulme, Jean Christophe. Universite Grenoble Alpes; Francia
Fil: Lanté, Fabien. Universite Grenoble Alpes; Francia
Fil: Arnal, Isabelle. Universite Grenoble Alpes; Francia
Fil: Buisson, Alain. Universite Grenoble Alpes; Francia
Fil: Goldberg, Yves. Universite Grenoble Alpes; Francia
Fil: Blanchoin, Laurent. Inserm; Francia. Universite Grenoble Alpes; Francia
Fil: Delphin, Christian. Universite Grenoble Alpes; Francia
Fil: Andrieux, Annie. Universite Grenoble Alpes; Francia
Materia
Dendritic Spines
MAP6
Actin
Neurons
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96846
Acceder
id CONICETDig_d00a58ff2c6c8e44c6d6149441d6006a
oai_identifier_str oai:ri.conicet.gov.ar:11336/96846
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spinesPeris, LeticiaBisbal, MarianoMartinez Hernandez, JoséSaoudi, YasminaJonckheere, JulieRolland, MartaSebastien, MurielBrocard, JacquesDenarier, EricBosc, ChristopherGuerin, ChristopheGory Fauré, SylvieDeloulme, Jean ChristopheLanté, FabienArnal, IsabelleBuisson, AlainGoldberg, YvesBlanchoin, LaurentDelphin, ChristianAndrieux, AnnieDendritic SpinesMAP6ActinNeuronshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Emerging evidence indicates that microtubule-associated proteins (MAPs) are implicated in synaptic function; in particular, mice deficient for MAP6 exhibit striking deficits in plasticity and cognition. How MAP6 connects to plasticity mechanisms is unclear. Here, we address the possible role of this protein in dendritic spines. We find that in MAP6-deficient cortical and hippocampal neurons, maintenance of mature spines is impaired, and can be restored by expressing a stretch of the MAP6 sequence called Mc modules. Mc modules directly bind actin filaments and mediate activity-dependent stabilisation of F-actin in dendritic spines, a key event of synaptic plasticity. In vitro, Mc modules enhance actin filament nucleation and promote the formation of stable, highly ordered filament bundles. Activity-induced phosphorylation of MAP6 likely controls its transfer to the spine cytoskeleton. These results provide a molecular explanation for the role of MAP6 in cognition, enlightening the connection between cytoskeletal dysfunction, synaptic impairment and neuropsychiatric illnesses.Fil: Peris, Leticia. Universite Grenoble Alpes; FranciaFil: Bisbal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universite Grenoble Alpes; FranciaFil: Martinez Hernandez, José. Universidad del País Vasco; España. Universite Grenoble Alpes; FranciaFil: Saoudi, Yasmina. Universite Grenoble Alpes; FranciaFil: Jonckheere, Julie. Universite Grenoble Alpes; FranciaFil: Rolland, Marta. Universite Grenoble Alpes; FranciaFil: Sebastien, Muriel. Universite Grenoble Alpes; FranciaFil: Brocard, Jacques. Universite Grenoble Alpes; FranciaFil: Denarier, Eric. Universite Grenoble Alpes; FranciaFil: Bosc, Christopher. Universite Grenoble Alpes; FranciaFil: Guerin, Christophe. Universite Grenoble Alpes; Francia. Inserm; FranciaFil: Gory Fauré, Sylvie. Universite Grenoble Alpes; FranciaFil: Deloulme, Jean Christophe. Universite Grenoble Alpes; FranciaFil: Lanté, Fabien. Universite Grenoble Alpes; FranciaFil: Arnal, Isabelle. Universite Grenoble Alpes; FranciaFil: Buisson, Alain. Universite Grenoble Alpes; FranciaFil: Goldberg, Yves. Universite Grenoble Alpes; FranciaFil: Blanchoin, Laurent. Inserm; Francia. Universite Grenoble Alpes; FranciaFil: Delphin, Christian. Universite Grenoble Alpes; FranciaFil: Andrieux, Annie. Universite Grenoble Alpes; FranciaNature Publishing Group2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96846Peris, Leticia; Bisbal, Mariano; Martinez Hernandez, José; Saoudi, Yasmina; Jonckheere, Julie; et al.; A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines; Nature Publishing Group; Nature Communications; 9; 1; 12-2018; 1-152041-1723CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41467-018-05869-zinfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-018-05869-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:05Zoai:ri.conicet.gov.ar:11336/96846instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:05.839CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines
title A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines
spellingShingle A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines
Peris, Leticia
Dendritic Spines
MAP6
Actin
Neurons
title_short A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines
title_full A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines
title_fullStr A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines
title_full_unstemmed A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines
title_sort A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines
dc.creator.none.fl_str_mv Peris, Leticia
Bisbal, Mariano
Martinez Hernandez, José
Saoudi, Yasmina
Jonckheere, Julie
Rolland, Marta
Sebastien, Muriel
Brocard, Jacques
Denarier, Eric
Bosc, Christopher
Guerin, Christophe
Gory Fauré, Sylvie
Deloulme, Jean Christophe
Lanté, Fabien
Arnal, Isabelle
Buisson, Alain
Goldberg, Yves
Blanchoin, Laurent
Delphin, Christian
Andrieux, Annie
author Peris, Leticia
author_facet Peris, Leticia
Bisbal, Mariano
Martinez Hernandez, José
Saoudi, Yasmina
Jonckheere, Julie
Rolland, Marta
Sebastien, Muriel
Brocard, Jacques
Denarier, Eric
Bosc, Christopher
Guerin, Christophe
Gory Fauré, Sylvie
Deloulme, Jean Christophe
Lanté, Fabien
Arnal, Isabelle
Buisson, Alain
Goldberg, Yves
Blanchoin, Laurent
Delphin, Christian
Andrieux, Annie
author_role author
author2 Bisbal, Mariano
Martinez Hernandez, José
Saoudi, Yasmina
Jonckheere, Julie
Rolland, Marta
Sebastien, Muriel
Brocard, Jacques
Denarier, Eric
Bosc, Christopher
Guerin, Christophe
Gory Fauré, Sylvie
Deloulme, Jean Christophe
Lanté, Fabien
Arnal, Isabelle
Buisson, Alain
Goldberg, Yves
Blanchoin, Laurent
Delphin, Christian
Andrieux, Annie
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Dendritic Spines
MAP6
Actin
Neurons
topic Dendritic Spines
MAP6
Actin
Neurons
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Emerging evidence indicates that microtubule-associated proteins (MAPs) are implicated in synaptic function; in particular, mice deficient for MAP6 exhibit striking deficits in plasticity and cognition. How MAP6 connects to plasticity mechanisms is unclear. Here, we address the possible role of this protein in dendritic spines. We find that in MAP6-deficient cortical and hippocampal neurons, maintenance of mature spines is impaired, and can be restored by expressing a stretch of the MAP6 sequence called Mc modules. Mc modules directly bind actin filaments and mediate activity-dependent stabilisation of F-actin in dendritic spines, a key event of synaptic plasticity. In vitro, Mc modules enhance actin filament nucleation and promote the formation of stable, highly ordered filament bundles. Activity-induced phosphorylation of MAP6 likely controls its transfer to the spine cytoskeleton. These results provide a molecular explanation for the role of MAP6 in cognition, enlightening the connection between cytoskeletal dysfunction, synaptic impairment and neuropsychiatric illnesses.
Fil: Peris, Leticia. Universite Grenoble Alpes; Francia
Fil: Bisbal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universite Grenoble Alpes; Francia
Fil: Martinez Hernandez, José. Universidad del País Vasco; España. Universite Grenoble Alpes; Francia
Fil: Saoudi, Yasmina. Universite Grenoble Alpes; Francia
Fil: Jonckheere, Julie. Universite Grenoble Alpes; Francia
Fil: Rolland, Marta. Universite Grenoble Alpes; Francia
Fil: Sebastien, Muriel. Universite Grenoble Alpes; Francia
Fil: Brocard, Jacques. Universite Grenoble Alpes; Francia
Fil: Denarier, Eric. Universite Grenoble Alpes; Francia
Fil: Bosc, Christopher. Universite Grenoble Alpes; Francia
Fil: Guerin, Christophe. Universite Grenoble Alpes; Francia. Inserm; Francia
Fil: Gory Fauré, Sylvie. Universite Grenoble Alpes; Francia
Fil: Deloulme, Jean Christophe. Universite Grenoble Alpes; Francia
Fil: Lanté, Fabien. Universite Grenoble Alpes; Francia
Fil: Arnal, Isabelle. Universite Grenoble Alpes; Francia
Fil: Buisson, Alain. Universite Grenoble Alpes; Francia
Fil: Goldberg, Yves. Universite Grenoble Alpes; Francia
Fil: Blanchoin, Laurent. Inserm; Francia. Universite Grenoble Alpes; Francia
Fil: Delphin, Christian. Universite Grenoble Alpes; Francia
Fil: Andrieux, Annie. Universite Grenoble Alpes; Francia
description Emerging evidence indicates that microtubule-associated proteins (MAPs) are implicated in synaptic function; in particular, mice deficient for MAP6 exhibit striking deficits in plasticity and cognition. How MAP6 connects to plasticity mechanisms is unclear. Here, we address the possible role of this protein in dendritic spines. We find that in MAP6-deficient cortical and hippocampal neurons, maintenance of mature spines is impaired, and can be restored by expressing a stretch of the MAP6 sequence called Mc modules. Mc modules directly bind actin filaments and mediate activity-dependent stabilisation of F-actin in dendritic spines, a key event of synaptic plasticity. In vitro, Mc modules enhance actin filament nucleation and promote the formation of stable, highly ordered filament bundles. Activity-induced phosphorylation of MAP6 likely controls its transfer to the spine cytoskeleton. These results provide a molecular explanation for the role of MAP6 in cognition, enlightening the connection between cytoskeletal dysfunction, synaptic impairment and neuropsychiatric illnesses.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96846
Peris, Leticia; Bisbal, Mariano; Martinez Hernandez, José; Saoudi, Yasmina; Jonckheere, Julie; et al.; A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines; Nature Publishing Group; Nature Communications; 9; 1; 12-2018; 1-15
2041-1723
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96846
identifier_str_mv Peris, Leticia; Bisbal, Mariano; Martinez Hernandez, José; Saoudi, Yasmina; Jonckheere, Julie; et al.; A key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines; Nature Publishing Group; Nature Communications; 9; 1; 12-2018; 1-15
2041-1723
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41467-018-05869-z
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-018-05869-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613089945714688
score 13.070432

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