Stress‐induced sensitization to cocaine: actin cytoskeleton remodeling within mesocorticolimbic nuclei

Autores
Esparza, Maria Alejandra; Bollati, Flavia Andrea; Garcia Keller, Constanza; Virgolini, Miriam Beatriz; Lopez, Lidia Margarita; Brusco, Herminia Alicia; Shen, Hao Wei; Kalivas, Peter W.; Cancela, Liliana Marina
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study investigated whether the adaptations induced by repeated cocaine in the actin cytoskeleton, the surface expression of AMPA receptor subunit GluR1, dendritic spine morphology and the size of postsynaptic density (PSD) in nucleus accumbens (NAc) and prefrontal cortex (Pfc) are relevant to how repeated immobilization stress induces behavioural cross-sensitization to cocaine. Wistar rats were restrained daily (2 hours) for 7 days, and three weeks later, the NAc and Pfc were dissected from animals 45 min following acute saline or cocaine (30 mg/kg i.p.). F-actin, actin-binding protein (ABP) and GluR1 were determined by western blotting, and dendritic spines and the size of PSD measured by electron microscopy. In the NAc, a decrease in p-cofilin and p-cortactin, and an increase in GluR1 and PSD size was found in the stress plus cocaine group, while in the Pfc an increase in F-actin and Arp2, as well as the number of spines, was observed in the stress group. Inhibition of actin cycling with latrunculin A in NAc restored expression of GluR1, and both latrunculin A and the AMPAR antagonist CNQX inhibited the expression of motor cross-sensitization to cocaine (15 mg/kg i.p.). This study shows that cross-sensitization is associated with changes in the actin cytoskeleton, the size of the PSD and GluR1 expression that partly parallel the neuroadaptations elicited by sensitization to repeated cocaine. Also, the actin dynamics regulating GluR1 expression in the NAc contribute to the expression of cross-sensitization between stress and cocaine, but the changes in Pfc were not associated with cross-sensitization.
Fil: Esparza, Maria Alejandra. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Bollati, Flavia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Garcia Keller, Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Virgolini, Miriam Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Lopez, Lidia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Brusco, Herminia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Shen, Hao Wei. Medical University of South Carolina; Estados Unidos
Fil: Kalivas, Peter W.. Medical University of South Carolina; Estados Unidos
Fil: Cancela, Liliana Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Materia
F-actin
dendritic spines
AMPA receptors
latrunculin A
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/274395
Acceder
id CONICETDig_2706743f42bc111b80e460d6929a60df
oai_identifier_str oai:ri.conicet.gov.ar:11336/274395
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Stress‐induced sensitization to cocaine: actin cytoskeleton remodeling within mesocorticolimbic nucleiEsparza, Maria AlejandraBollati, Flavia AndreaGarcia Keller, ConstanzaVirgolini, Miriam BeatrizLopez, Lidia MargaritaBrusco, Herminia AliciaShen, Hao WeiKalivas, Peter W.Cancela, Liliana MarinaF-actindendritic spinesAMPA receptorslatrunculin Ahttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3This study investigated whether the adaptations induced by repeated cocaine in the actin cytoskeleton, the surface expression of AMPA receptor subunit GluR1, dendritic spine morphology and the size of postsynaptic density (PSD) in nucleus accumbens (NAc) and prefrontal cortex (Pfc) are relevant to how repeated immobilization stress induces behavioural cross-sensitization to cocaine. Wistar rats were restrained daily (2 hours) for 7 days, and three weeks later, the NAc and Pfc were dissected from animals 45 min following acute saline or cocaine (30 mg/kg i.p.). F-actin, actin-binding protein (ABP) and GluR1 were determined by western blotting, and dendritic spines and the size of PSD measured by electron microscopy. In the NAc, a decrease in p-cofilin and p-cortactin, and an increase in GluR1 and PSD size was found in the stress plus cocaine group, while in the Pfc an increase in F-actin and Arp2, as well as the number of spines, was observed in the stress group. Inhibition of actin cycling with latrunculin A in NAc restored expression of GluR1, and both latrunculin A and the AMPAR antagonist CNQX inhibited the expression of motor cross-sensitization to cocaine (15 mg/kg i.p.). This study shows that cross-sensitization is associated with changes in the actin cytoskeleton, the size of the PSD and GluR1 expression that partly parallel the neuroadaptations elicited by sensitization to repeated cocaine. Also, the actin dynamics regulating GluR1 expression in the NAc contribute to the expression of cross-sensitization between stress and cocaine, but the changes in Pfc were not associated with cross-sensitization.Fil: Esparza, Maria Alejandra. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Bollati, Flavia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Garcia Keller, Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Virgolini, Miriam Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Lopez, Lidia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Brusco, Herminia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Shen, Hao Wei. Medical University of South Carolina; Estados UnidosFil: Kalivas, Peter W.. Medical University of South Carolina; Estados UnidosFil: Cancela, Liliana Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaWiley Blackwell Publishing, Inc2012-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/274395Esparza, Maria Alejandra; Bollati, Flavia Andrea; Garcia Keller, Constanza; Virgolini, Miriam Beatriz; Lopez, Lidia Margarita; et al.; Stress‐induced sensitization to cocaine: actin cytoskeleton remodeling within mesocorticolimbic nuclei; Wiley Blackwell Publishing, Inc; European Journal of Neuroscience; 36; 8; 6-2012; 3103-31170953-816XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1460-9568.2012.08239.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1460-9568.2012.08239.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:21:45Zoai:ri.conicet.gov.ar:11336/274395instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:21:45.936CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Stress‐induced sensitization to cocaine: actin cytoskeleton remodeling within mesocorticolimbic nuclei
title Stress‐induced sensitization to cocaine: actin cytoskeleton remodeling within mesocorticolimbic nuclei
spellingShingle Stress‐induced sensitization to cocaine: actin cytoskeleton remodeling within mesocorticolimbic nuclei
Esparza, Maria Alejandra
F-actin
dendritic spines
AMPA receptors
latrunculin A
title_short Stress‐induced sensitization to cocaine: actin cytoskeleton remodeling within mesocorticolimbic nuclei
title_full Stress‐induced sensitization to cocaine: actin cytoskeleton remodeling within mesocorticolimbic nuclei
title_fullStr Stress‐induced sensitization to cocaine: actin cytoskeleton remodeling within mesocorticolimbic nuclei
title_full_unstemmed Stress‐induced sensitization to cocaine: actin cytoskeleton remodeling within mesocorticolimbic nuclei
title_sort Stress‐induced sensitization to cocaine: actin cytoskeleton remodeling within mesocorticolimbic nuclei
dc.creator.none.fl_str_mv Esparza, Maria Alejandra
Bollati, Flavia Andrea
Garcia Keller, Constanza
Virgolini, Miriam Beatriz
Lopez, Lidia Margarita
Brusco, Herminia Alicia
Shen, Hao Wei
Kalivas, Peter W.
Cancela, Liliana Marina
author Esparza, Maria Alejandra
author_facet Esparza, Maria Alejandra
Bollati, Flavia Andrea
Garcia Keller, Constanza
Virgolini, Miriam Beatriz
Lopez, Lidia Margarita
Brusco, Herminia Alicia
Shen, Hao Wei
Kalivas, Peter W.
Cancela, Liliana Marina
author_role author
author2 Bollati, Flavia Andrea
Garcia Keller, Constanza
Virgolini, Miriam Beatriz
Lopez, Lidia Margarita
Brusco, Herminia Alicia
Shen, Hao Wei
Kalivas, Peter W.
Cancela, Liliana Marina
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv F-actin
dendritic spines
AMPA receptors
latrunculin A
topic F-actin
dendritic spines
AMPA receptors
latrunculin A
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv This study investigated whether the adaptations induced by repeated cocaine in the actin cytoskeleton, the surface expression of AMPA receptor subunit GluR1, dendritic spine morphology and the size of postsynaptic density (PSD) in nucleus accumbens (NAc) and prefrontal cortex (Pfc) are relevant to how repeated immobilization stress induces behavioural cross-sensitization to cocaine. Wistar rats were restrained daily (2 hours) for 7 days, and three weeks later, the NAc and Pfc were dissected from animals 45 min following acute saline or cocaine (30 mg/kg i.p.). F-actin, actin-binding protein (ABP) and GluR1 were determined by western blotting, and dendritic spines and the size of PSD measured by electron microscopy. In the NAc, a decrease in p-cofilin and p-cortactin, and an increase in GluR1 and PSD size was found in the stress plus cocaine group, while in the Pfc an increase in F-actin and Arp2, as well as the number of spines, was observed in the stress group. Inhibition of actin cycling with latrunculin A in NAc restored expression of GluR1, and both latrunculin A and the AMPAR antagonist CNQX inhibited the expression of motor cross-sensitization to cocaine (15 mg/kg i.p.). This study shows that cross-sensitization is associated with changes in the actin cytoskeleton, the size of the PSD and GluR1 expression that partly parallel the neuroadaptations elicited by sensitization to repeated cocaine. Also, the actin dynamics regulating GluR1 expression in the NAc contribute to the expression of cross-sensitization between stress and cocaine, but the changes in Pfc were not associated with cross-sensitization.
Fil: Esparza, Maria Alejandra. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Bollati, Flavia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Garcia Keller, Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Virgolini, Miriam Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Lopez, Lidia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Brusco, Herminia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Shen, Hao Wei. Medical University of South Carolina; Estados Unidos
Fil: Kalivas, Peter W.. Medical University of South Carolina; Estados Unidos
Fil: Cancela, Liliana Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
description This study investigated whether the adaptations induced by repeated cocaine in the actin cytoskeleton, the surface expression of AMPA receptor subunit GluR1, dendritic spine morphology and the size of postsynaptic density (PSD) in nucleus accumbens (NAc) and prefrontal cortex (Pfc) are relevant to how repeated immobilization stress induces behavioural cross-sensitization to cocaine. Wistar rats were restrained daily (2 hours) for 7 days, and three weeks later, the NAc and Pfc were dissected from animals 45 min following acute saline or cocaine (30 mg/kg i.p.). F-actin, actin-binding protein (ABP) and GluR1 were determined by western blotting, and dendritic spines and the size of PSD measured by electron microscopy. In the NAc, a decrease in p-cofilin and p-cortactin, and an increase in GluR1 and PSD size was found in the stress plus cocaine group, while in the Pfc an increase in F-actin and Arp2, as well as the number of spines, was observed in the stress group. Inhibition of actin cycling with latrunculin A in NAc restored expression of GluR1, and both latrunculin A and the AMPAR antagonist CNQX inhibited the expression of motor cross-sensitization to cocaine (15 mg/kg i.p.). This study shows that cross-sensitization is associated with changes in the actin cytoskeleton, the size of the PSD and GluR1 expression that partly parallel the neuroadaptations elicited by sensitization to repeated cocaine. Also, the actin dynamics regulating GluR1 expression in the NAc contribute to the expression of cross-sensitization between stress and cocaine, but the changes in Pfc were not associated with cross-sensitization.
publishDate 2012
dc.date.none.fl_str_mv 2012-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/274395
Esparza, Maria Alejandra; Bollati, Flavia Andrea; Garcia Keller, Constanza; Virgolini, Miriam Beatriz; Lopez, Lidia Margarita; et al.; Stress‐induced sensitization to cocaine: actin cytoskeleton remodeling within mesocorticolimbic nuclei; Wiley Blackwell Publishing, Inc; European Journal of Neuroscience; 36; 8; 6-2012; 3103-3117
0953-816X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/274395
identifier_str_mv Esparza, Maria Alejandra; Bollati, Flavia Andrea; Garcia Keller, Constanza; Virgolini, Miriam Beatriz; Lopez, Lidia Margarita; et al.; Stress‐induced sensitization to cocaine: actin cytoskeleton remodeling within mesocorticolimbic nuclei; Wiley Blackwell Publishing, Inc; European Journal of Neuroscience; 36; 8; 6-2012; 3103-3117
0953-816X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1460-9568.2012.08239.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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