Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia

Autores
Saraceno, Gustavo Ezequiel; Castilla Lozano, Maria del Rocio; Barreto, G. E.; Gonzalez, J.; Kolliker Frers, Rodolfo Alberto; Capani, Francisco
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Perinatal asphyxia (PA) affects the synaptic function and morphological organization. In previous works we have shown neuronal and synaptic changes in rat neostriatum subjected to hypoxia leading to long term ubi-protein accumulation. Since F-actin is highly concentrated in dendritic spines, modifications in this proteins their organization could be related with alterations induced by hypoxia in the Central Nervous System. In the present study, we investigate the effects of PA on the actin cytoskeleton of hippocampal post-synaptic densities (PSD) in 4-month-old rats. PSD showed an increment in their thickness and in the level of ubiquitination. Correlative fluorescence-electron microscopy photooxidation showed a decrease in the number of F-actin stained spines in hippocampal excitatory synapses subjected to PA. Although Western blot analysis also showed a sligth decreased in beta-actin in PSD in PA animals the difference was not significant. Take together, this data suggests that long term actin cytoskeleton might have role in PSD alterations which would be a spread phenomena induced by PA.
Fil: Saraceno, Gustavo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Castilla Lozano, Maria del Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Barreto, G. E.. Pontificia Universidad Javeriana; Colombia
Fil: Gonzalez, J.. Pontificia Universidad Javeriana; Colombia
Fil: Kolliker Frers, Rodolfo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Materia
dendritic spines
hypoxia
actin cytoskeleton
hypoccampus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/269514
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/269514
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Hippocampal Dendritic Spines Modifications Induced by Perinatal AsphyxiaSaraceno, Gustavo EzequielCastilla Lozano, Maria del RocioBarreto, G. E.Gonzalez, J.Kolliker Frers, Rodolfo AlbertoCapani, Franciscodendritic spineshypoxiaactin cytoskeletonhypoccampushttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Perinatal asphyxia (PA) affects the synaptic function and morphological organization. In previous works we have shown neuronal and synaptic changes in rat neostriatum subjected to hypoxia leading to long term ubi-protein accumulation. Since F-actin is highly concentrated in dendritic spines, modifications in this proteins their organization could be related with alterations induced by hypoxia in the Central Nervous System. In the present study, we investigate the effects of PA on the actin cytoskeleton of hippocampal post-synaptic densities (PSD) in 4-month-old rats. PSD showed an increment in their thickness and in the level of ubiquitination. Correlative fluorescence-electron microscopy photooxidation showed a decrease in the number of F-actin stained spines in hippocampal excitatory synapses subjected to PA. Although Western blot analysis also showed a sligth decreased in beta-actin in PSD in PA animals the difference was not significant. Take together, this data suggests that long term actin cytoskeleton might have role in PSD alterations which would be a spread phenomena induced by PA.Fil: Saraceno, Gustavo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Castilla Lozano, Maria del Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Barreto, G. E.. Pontificia Universidad Javeriana; ColombiaFil: Gonzalez, J.. Pontificia Universidad Javeriana; ColombiaFil: Kolliker Frers, Rodolfo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaHindawi Publishing Corporation2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/269514Saraceno, Gustavo Ezequiel; Castilla Lozano, Maria del Rocio; Barreto, G. E.; Gonzalez, J.; Kolliker Frers, Rodolfo Alberto; et al.; Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia; Hindawi Publishing Corporation; Neural Plasticity; 2012; 5-2012; 1-101687-5443CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1155/2012/873532info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1155/2012/873532info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:23Zoai:ri.conicet.gov.ar:11336/269514instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:24.135CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia
title Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia
spellingShingle Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia
Saraceno, Gustavo Ezequiel
dendritic spines
hypoxia
actin cytoskeleton
hypoccampus
title_short Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia
title_full Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia
title_fullStr Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia
title_full_unstemmed Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia
title_sort Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia
dc.creator.none.fl_str_mv Saraceno, Gustavo Ezequiel
Castilla Lozano, Maria del Rocio
Barreto, G. E.
Gonzalez, J.
Kolliker Frers, Rodolfo Alberto
Capani, Francisco
author Saraceno, Gustavo Ezequiel
author_facet Saraceno, Gustavo Ezequiel
Castilla Lozano, Maria del Rocio
Barreto, G. E.
Gonzalez, J.
Kolliker Frers, Rodolfo Alberto
Capani, Francisco
author_role author
author2 Castilla Lozano, Maria del Rocio
Barreto, G. E.
Gonzalez, J.
Kolliker Frers, Rodolfo Alberto
Capani, Francisco
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv dendritic spines
hypoxia
actin cytoskeleton
hypoccampus
topic dendritic spines
hypoxia
actin cytoskeleton
hypoccampus
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Perinatal asphyxia (PA) affects the synaptic function and morphological organization. In previous works we have shown neuronal and synaptic changes in rat neostriatum subjected to hypoxia leading to long term ubi-protein accumulation. Since F-actin is highly concentrated in dendritic spines, modifications in this proteins their organization could be related with alterations induced by hypoxia in the Central Nervous System. In the present study, we investigate the effects of PA on the actin cytoskeleton of hippocampal post-synaptic densities (PSD) in 4-month-old rats. PSD showed an increment in their thickness and in the level of ubiquitination. Correlative fluorescence-electron microscopy photooxidation showed a decrease in the number of F-actin stained spines in hippocampal excitatory synapses subjected to PA. Although Western blot analysis also showed a sligth decreased in beta-actin in PSD in PA animals the difference was not significant. Take together, this data suggests that long term actin cytoskeleton might have role in PSD alterations which would be a spread phenomena induced by PA.
Fil: Saraceno, Gustavo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Castilla Lozano, Maria del Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Barreto, G. E.. Pontificia Universidad Javeriana; Colombia
Fil: Gonzalez, J.. Pontificia Universidad Javeriana; Colombia
Fil: Kolliker Frers, Rodolfo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
description Perinatal asphyxia (PA) affects the synaptic function and morphological organization. In previous works we have shown neuronal and synaptic changes in rat neostriatum subjected to hypoxia leading to long term ubi-protein accumulation. Since F-actin is highly concentrated in dendritic spines, modifications in this proteins their organization could be related with alterations induced by hypoxia in the Central Nervous System. In the present study, we investigate the effects of PA on the actin cytoskeleton of hippocampal post-synaptic densities (PSD) in 4-month-old rats. PSD showed an increment in their thickness and in the level of ubiquitination. Correlative fluorescence-electron microscopy photooxidation showed a decrease in the number of F-actin stained spines in hippocampal excitatory synapses subjected to PA. Although Western blot analysis also showed a sligth decreased in beta-actin in PSD in PA animals the difference was not significant. Take together, this data suggests that long term actin cytoskeleton might have role in PSD alterations which would be a spread phenomena induced by PA.
publishDate 2012
dc.date.none.fl_str_mv 2012-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/269514
Saraceno, Gustavo Ezequiel; Castilla Lozano, Maria del Rocio; Barreto, G. E.; Gonzalez, J.; Kolliker Frers, Rodolfo Alberto; et al.; Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia; Hindawi Publishing Corporation; Neural Plasticity; 2012; 5-2012; 1-10
1687-5443
CONICET Digital
CONICET
url http://hdl.handle.net/11336/269514
identifier_str_mv Saraceno, Gustavo Ezequiel; Castilla Lozano, Maria del Rocio; Barreto, G. E.; Gonzalez, J.; Kolliker Frers, Rodolfo Alberto; et al.; Hippocampal Dendritic Spines Modifications Induced by Perinatal Asphyxia; Hindawi Publishing Corporation; Neural Plasticity; 2012; 5-2012; 1-10
1687-5443
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1155/2012/873532
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1155/2012/873532
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269635724967936
score 13.13397

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