Science based calibration for the extraction of 'analyte-specific' HPLC-DAD chromatograms in environmental analysis

Autores
Kuligowski, J.; Martínez Galera, M.; Gil García, M. D.; Culzoni, Maria Julia; Goicoechea, Hector Casimiro; Garrigues, S.; Quintás, G.; de la Guardia, Miguel
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Multivariate science based calibration (SBC) has been applied to the resolution of overlapped peaks in liquid chromatography with diode array detection (LC-DAD). Complex river water samples spiked with 11 pharmaceutical substances resulted in poorly resolved chromatograms containing additional peaks from interfering matrix compounds and a change in the background absorbance due to the mobile phase gradient. Applying the present multivariate approach it was possible to resolve all 11 analytes from overlapping peaks, obtaining linear calibration lines (R2 > 0.96). Recovery percentages on spiked samples ranged between 74.6 and 113.5%, which are quite satisfactory taking into account the low concentration ranges considered to 1-7 μg L-1.
Fil: Kuligowski, J.. Universidad de Valencia; España
Fil: Martínez Galera, M.. Universidad de Almería; España
Fil: Gil García, M. D.. Universidad de Almería; España
Fil: Culzoni, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Garrigues, S.. Universidad de Valencia; España
Fil: Quintás, G.. Universidad de Valencia; España
Fil: de la Guardia, Miguel. Universidad de Valencia; España
Materia
Environmental Analysis
High Performance Liquid Chromatography-Diode Array Detection (Hplc-Dad)
Science Based Calibration
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/74459
Acceder
id CONICETDig_ce4d17814bb6839a72f90aaeef0967dc
oai_identifier_str oai:ri.conicet.gov.ar:11336/74459
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Science based calibration for the extraction of 'analyte-specific' HPLC-DAD chromatograms in environmental analysisKuligowski, J.Martínez Galera, M.Gil García, M. D.Culzoni, Maria JuliaGoicoechea, Hector CasimiroGarrigues, S.Quintás, G.de la Guardia, MiguelEnvironmental AnalysisHigh Performance Liquid Chromatography-Diode Array Detection (Hplc-Dad)Science Based Calibrationhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Multivariate science based calibration (SBC) has been applied to the resolution of overlapped peaks in liquid chromatography with diode array detection (LC-DAD). Complex river water samples spiked with 11 pharmaceutical substances resulted in poorly resolved chromatograms containing additional peaks from interfering matrix compounds and a change in the background absorbance due to the mobile phase gradient. Applying the present multivariate approach it was possible to resolve all 11 analytes from overlapping peaks, obtaining linear calibration lines (R2 > 0.96). Recovery percentages on spiked samples ranged between 74.6 and 113.5%, which are quite satisfactory taking into account the low concentration ranges considered to 1-7 μg L-1.Fil: Kuligowski, J.. Universidad de Valencia; EspañaFil: Martínez Galera, M.. Universidad de Almería; EspañaFil: Gil García, M. D.. Universidad de Almería; EspañaFil: Culzoni, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Garrigues, S.. Universidad de Valencia; EspañaFil: Quintás, G.. Universidad de Valencia; EspañaFil: de la Guardia, Miguel. Universidad de Valencia; EspañaElsevier Science2011-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/74459Kuligowski, J.; Martínez Galera, M.; Gil García, M. D.; Culzoni, Maria Julia; Goicoechea, Hector Casimiro; et al.; Science based calibration for the extraction of 'analyte-specific' HPLC-DAD chromatograms in environmental analysis; Elsevier Science; Talanta; 83; 4; 1-2011; 1158-11650039-9140CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2010.07.060info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0039914010005904info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:10:39Zoai:ri.conicet.gov.ar:11336/74459instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:10:39.87CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Science based calibration for the extraction of 'analyte-specific' HPLC-DAD chromatograms in environmental analysis
title Science based calibration for the extraction of 'analyte-specific' HPLC-DAD chromatograms in environmental analysis
spellingShingle Science based calibration for the extraction of 'analyte-specific' HPLC-DAD chromatograms in environmental analysis
Kuligowski, J.
Environmental Analysis
High Performance Liquid Chromatography-Diode Array Detection (Hplc-Dad)
Science Based Calibration
title_short Science based calibration for the extraction of 'analyte-specific' HPLC-DAD chromatograms in environmental analysis
title_full Science based calibration for the extraction of 'analyte-specific' HPLC-DAD chromatograms in environmental analysis
title_fullStr Science based calibration for the extraction of 'analyte-specific' HPLC-DAD chromatograms in environmental analysis
title_full_unstemmed Science based calibration for the extraction of 'analyte-specific' HPLC-DAD chromatograms in environmental analysis
title_sort Science based calibration for the extraction of 'analyte-specific' HPLC-DAD chromatograms in environmental analysis
dc.creator.none.fl_str_mv Kuligowski, J.
Martínez Galera, M.
Gil García, M. D.
Culzoni, Maria Julia
Goicoechea, Hector Casimiro
Garrigues, S.
Quintás, G.
de la Guardia, Miguel
author Kuligowski, J.
author_facet Kuligowski, J.
Martínez Galera, M.
Gil García, M. D.
Culzoni, Maria Julia
Goicoechea, Hector Casimiro
Garrigues, S.
Quintás, G.
de la Guardia, Miguel
author_role author
author2 Martínez Galera, M.
Gil García, M. D.
Culzoni, Maria Julia
Goicoechea, Hector Casimiro
Garrigues, S.
Quintás, G.
de la Guardia, Miguel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Environmental Analysis
High Performance Liquid Chromatography-Diode Array Detection (Hplc-Dad)
Science Based Calibration
topic Environmental Analysis
High Performance Liquid Chromatography-Diode Array Detection (Hplc-Dad)
Science Based Calibration
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Multivariate science based calibration (SBC) has been applied to the resolution of overlapped peaks in liquid chromatography with diode array detection (LC-DAD). Complex river water samples spiked with 11 pharmaceutical substances resulted in poorly resolved chromatograms containing additional peaks from interfering matrix compounds and a change in the background absorbance due to the mobile phase gradient. Applying the present multivariate approach it was possible to resolve all 11 analytes from overlapping peaks, obtaining linear calibration lines (R2 > 0.96). Recovery percentages on spiked samples ranged between 74.6 and 113.5%, which are quite satisfactory taking into account the low concentration ranges considered to 1-7 μg L-1.
Fil: Kuligowski, J.. Universidad de Valencia; España
Fil: Martínez Galera, M.. Universidad de Almería; España
Fil: Gil García, M. D.. Universidad de Almería; España
Fil: Culzoni, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Garrigues, S.. Universidad de Valencia; España
Fil: Quintás, G.. Universidad de Valencia; España
Fil: de la Guardia, Miguel. Universidad de Valencia; España
description Multivariate science based calibration (SBC) has been applied to the resolution of overlapped peaks in liquid chromatography with diode array detection (LC-DAD). Complex river water samples spiked with 11 pharmaceutical substances resulted in poorly resolved chromatograms containing additional peaks from interfering matrix compounds and a change in the background absorbance due to the mobile phase gradient. Applying the present multivariate approach it was possible to resolve all 11 analytes from overlapping peaks, obtaining linear calibration lines (R2 > 0.96). Recovery percentages on spiked samples ranged between 74.6 and 113.5%, which are quite satisfactory taking into account the low concentration ranges considered to 1-7 μg L-1.
publishDate 2011
dc.date.none.fl_str_mv 2011-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/74459
Kuligowski, J.; Martínez Galera, M.; Gil García, M. D.; Culzoni, Maria Julia; Goicoechea, Hector Casimiro; et al.; Science based calibration for the extraction of 'analyte-specific' HPLC-DAD chromatograms in environmental analysis; Elsevier Science; Talanta; 83; 4; 1-2011; 1158-1165
0039-9140
CONICET Digital
CONICET
url http://hdl.handle.net/11336/74459
identifier_str_mv Kuligowski, J.; Martínez Galera, M.; Gil García, M. D.; Culzoni, Maria Julia; Goicoechea, Hector Casimiro; et al.; Science based calibration for the extraction of 'analyte-specific' HPLC-DAD chromatograms in environmental analysis; Elsevier Science; Talanta; 83; 4; 1-2011; 1158-1165
0039-9140
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2010.07.060
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0039914010005904
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083255241015296
score 13.22299

Publicaciones similares