Live Imaging of Axolotl Digit Regeneration Reveals Spatiotemporal Choreography of Diverse Connective Tissue Progenitor Pools

Autores
Currie, Joshua D.; Kawaguchi, Akane; Traspas, Ricardo Moreno; Schuez, Maritta; Chara, Osvaldo; Tanaka, Elly M.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Connective tissues—skeleton, dermis, pericytes, fascia—are a key cell source for regenerating the patterned skeleton during axolotl appendage regeneration. This complexity has made it difficult to identify the cells that regenerate skeletal tissue. Inability to identify these cells has impeded a mechanistic understanding of blastema formation. By tracing cells during digit tip regeneration using brainbow transgenic axolotls, we show that cells from each connective tissue compartment have distinct spatial and temporal profiles of proliferation, migration, and differentiation. Chondrocytes proliferate but do not migrate into the regenerate. In contrast, pericytes proliferate, then migrate into the blastema and give rise solely to pericytes. Periskeletal cells and fibroblasts contribute the bulk of digit blastema cells and acquire diverse fates according to successive waves of migration that choreograph their proximal-distal and tissue contributions. We further show that platelet-derived growth factor signaling is a potent inducer of fibroblast migration, which is required to form the blastema.
Fil: Currie, Joshua D.. Technische Universität Dresden; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania
Fil: Kawaguchi, Akane. Technische Universität Dresden; Alemania
Fil: Traspas, Ricardo Moreno. Technische Universität Dresden; Alemania
Fil: Schuez, Maritta. Technische Universität Dresden; Alemania
Fil: Chara, Osvaldo. Technische Universität Dresden; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina
Fil: Tanaka, Elly M.. Technische Universität Dresden; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania
Materia
AXOLOTL
BLASTEMA FORMATION
BRAINBOW
CELL MIGRATION
CONNECTIVE TISSUE
LIMB REGENERATION
LIVE IMAGING
PDGF SIGNALING
SKELETAL REGENERATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47998
Acceder
id CONICETDig_ce35439a2bef5208b7cb1330d418d666
oai_identifier_str oai:ri.conicet.gov.ar:11336/47998
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Live Imaging of Axolotl Digit Regeneration Reveals Spatiotemporal Choreography of Diverse Connective Tissue Progenitor PoolsCurrie, Joshua D.Kawaguchi, AkaneTraspas, Ricardo MorenoSchuez, MarittaChara, OsvaldoTanaka, Elly M.AXOLOTLBLASTEMA FORMATIONBRAINBOWCELL MIGRATIONCONNECTIVE TISSUELIMB REGENERATIONLIVE IMAGINGPDGF SIGNALINGSKELETAL REGENERATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Connective tissues—skeleton, dermis, pericytes, fascia—are a key cell source for regenerating the patterned skeleton during axolotl appendage regeneration. This complexity has made it difficult to identify the cells that regenerate skeletal tissue. Inability to identify these cells has impeded a mechanistic understanding of blastema formation. By tracing cells during digit tip regeneration using brainbow transgenic axolotls, we show that cells from each connective tissue compartment have distinct spatial and temporal profiles of proliferation, migration, and differentiation. Chondrocytes proliferate but do not migrate into the regenerate. In contrast, pericytes proliferate, then migrate into the blastema and give rise solely to pericytes. Periskeletal cells and fibroblasts contribute the bulk of digit blastema cells and acquire diverse fates according to successive waves of migration that choreograph their proximal-distal and tissue contributions. We further show that platelet-derived growth factor signaling is a potent inducer of fibroblast migration, which is required to form the blastema.Fil: Currie, Joshua D.. Technische Universität Dresden; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; AlemaniaFil: Kawaguchi, Akane. Technische Universität Dresden; AlemaniaFil: Traspas, Ricardo Moreno. Technische Universität Dresden; AlemaniaFil: Schuez, Maritta. Technische Universität Dresden; AlemaniaFil: Chara, Osvaldo. Technische Universität Dresden; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; ArgentinaFil: Tanaka, Elly M.. Technische Universität Dresden; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; AlemaniaCell Press2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47998Currie, Joshua D.; Kawaguchi, Akane; Traspas, Ricardo Moreno; Schuez, Maritta; Chara, Osvaldo; et al.; Live Imaging of Axolotl Digit Regeneration Reveals Spatiotemporal Choreography of Diverse Connective Tissue Progenitor Pools; Cell Press; Developmental Cell; 39; 4; 11-2016; 411-4231534-5807CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.devcel.2016.10.013info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1534580716307511info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:59:52Zoai:ri.conicet.gov.ar:11336/47998instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:59:53.197CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Live Imaging of Axolotl Digit Regeneration Reveals Spatiotemporal Choreography of Diverse Connective Tissue Progenitor Pools
title Live Imaging of Axolotl Digit Regeneration Reveals Spatiotemporal Choreography of Diverse Connective Tissue Progenitor Pools
spellingShingle Live Imaging of Axolotl Digit Regeneration Reveals Spatiotemporal Choreography of Diverse Connective Tissue Progenitor Pools
Currie, Joshua D.
AXOLOTL
BLASTEMA FORMATION
BRAINBOW
CELL MIGRATION
CONNECTIVE TISSUE
LIMB REGENERATION
LIVE IMAGING
PDGF SIGNALING
SKELETAL REGENERATION
title_short Live Imaging of Axolotl Digit Regeneration Reveals Spatiotemporal Choreography of Diverse Connective Tissue Progenitor Pools
title_full Live Imaging of Axolotl Digit Regeneration Reveals Spatiotemporal Choreography of Diverse Connective Tissue Progenitor Pools
title_fullStr Live Imaging of Axolotl Digit Regeneration Reveals Spatiotemporal Choreography of Diverse Connective Tissue Progenitor Pools
title_full_unstemmed Live Imaging of Axolotl Digit Regeneration Reveals Spatiotemporal Choreography of Diverse Connective Tissue Progenitor Pools
title_sort Live Imaging of Axolotl Digit Regeneration Reveals Spatiotemporal Choreography of Diverse Connective Tissue Progenitor Pools
dc.creator.none.fl_str_mv Currie, Joshua D.
Kawaguchi, Akane
Traspas, Ricardo Moreno
Schuez, Maritta
Chara, Osvaldo
Tanaka, Elly M.
author Currie, Joshua D.
author_facet Currie, Joshua D.
Kawaguchi, Akane
Traspas, Ricardo Moreno
Schuez, Maritta
Chara, Osvaldo
Tanaka, Elly M.
author_role author
author2 Kawaguchi, Akane
Traspas, Ricardo Moreno
Schuez, Maritta
Chara, Osvaldo
Tanaka, Elly M.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv AXOLOTL
BLASTEMA FORMATION
BRAINBOW
CELL MIGRATION
CONNECTIVE TISSUE
LIMB REGENERATION
LIVE IMAGING
PDGF SIGNALING
SKELETAL REGENERATION
topic AXOLOTL
BLASTEMA FORMATION
BRAINBOW
CELL MIGRATION
CONNECTIVE TISSUE
LIMB REGENERATION
LIVE IMAGING
PDGF SIGNALING
SKELETAL REGENERATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Connective tissues—skeleton, dermis, pericytes, fascia—are a key cell source for regenerating the patterned skeleton during axolotl appendage regeneration. This complexity has made it difficult to identify the cells that regenerate skeletal tissue. Inability to identify these cells has impeded a mechanistic understanding of blastema formation. By tracing cells during digit tip regeneration using brainbow transgenic axolotls, we show that cells from each connective tissue compartment have distinct spatial and temporal profiles of proliferation, migration, and differentiation. Chondrocytes proliferate but do not migrate into the regenerate. In contrast, pericytes proliferate, then migrate into the blastema and give rise solely to pericytes. Periskeletal cells and fibroblasts contribute the bulk of digit blastema cells and acquire diverse fates according to successive waves of migration that choreograph their proximal-distal and tissue contributions. We further show that platelet-derived growth factor signaling is a potent inducer of fibroblast migration, which is required to form the blastema.
Fil: Currie, Joshua D.. Technische Universität Dresden; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania
Fil: Kawaguchi, Akane. Technische Universität Dresden; Alemania
Fil: Traspas, Ricardo Moreno. Technische Universität Dresden; Alemania
Fil: Schuez, Maritta. Technische Universität Dresden; Alemania
Fil: Chara, Osvaldo. Technische Universität Dresden; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina
Fil: Tanaka, Elly M.. Technische Universität Dresden; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania
description Connective tissues—skeleton, dermis, pericytes, fascia—are a key cell source for regenerating the patterned skeleton during axolotl appendage regeneration. This complexity has made it difficult to identify the cells that regenerate skeletal tissue. Inability to identify these cells has impeded a mechanistic understanding of blastema formation. By tracing cells during digit tip regeneration using brainbow transgenic axolotls, we show that cells from each connective tissue compartment have distinct spatial and temporal profiles of proliferation, migration, and differentiation. Chondrocytes proliferate but do not migrate into the regenerate. In contrast, pericytes proliferate, then migrate into the blastema and give rise solely to pericytes. Periskeletal cells and fibroblasts contribute the bulk of digit blastema cells and acquire diverse fates according to successive waves of migration that choreograph their proximal-distal and tissue contributions. We further show that platelet-derived growth factor signaling is a potent inducer of fibroblast migration, which is required to form the blastema.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47998
Currie, Joshua D.; Kawaguchi, Akane; Traspas, Ricardo Moreno; Schuez, Maritta; Chara, Osvaldo; et al.; Live Imaging of Axolotl Digit Regeneration Reveals Spatiotemporal Choreography of Diverse Connective Tissue Progenitor Pools; Cell Press; Developmental Cell; 39; 4; 11-2016; 411-423
1534-5807
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47998
identifier_str_mv Currie, Joshua D.; Kawaguchi, Akane; Traspas, Ricardo Moreno; Schuez, Maritta; Chara, Osvaldo; et al.; Live Imaging of Axolotl Digit Regeneration Reveals Spatiotemporal Choreography of Diverse Connective Tissue Progenitor Pools; Cell Press; Developmental Cell; 39; 4; 11-2016; 411-423
1534-5807
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.devcel.2016.10.013
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1534580716307511
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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