Manganese Cytotoxicity Assay on Hippocampal Neuronal Cell Culture

Autores
Daoust, Alexia; Saoudi, Yasmina; Brocard, Jacques; Collomb, Nora; Batandier, Cecile; Bisbal, Mariano; Salome, Murielle; Andrieux, Annie; Bohic, Sylvain; Barbier, Emmanuel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Compared to an in vivo experiment, neuronal cell cultures are immediately accessible
to observation and manipulation. In this protocol, we describe a technique to evaluate the
cytotoxicity of a metal, manganese (Mn2+), on hippocampal neuronal cell cultures. Interestingly, this protocol is easily adaptable to any type of primary culture (e.g., cortical neurons) and any type of toxic compound (e.g., chemical product).
Fil: Daoust, Alexia. Inserm; Francia. Universite Joseph Fourier; Francia
Fil: Saoudi, Yasmina. Inserm; Francia. Universite Joseph Fourier; Francia
Fil: Brocard, Jacques. Inserm; Francia. Universite Joseph Fourier; Francia
Fil: Collomb, Nora. Inserm; Francia. Universite Joseph Fourier; Francia
Fil: Batandier, Cecile. Laboratoire de Bioénergétique Fondamentale et Appliquée; Francia
Fil: Bisbal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Inserm; Francia. Universite Joseph Fourier; Francia
Fil: Salome, Murielle. European Synchrotron Radiation Facility; Francia
Fil: Andrieux, Annie. Inserm; Francia. Universite Joseph Fourier; Francia
Fil: Bohic, Sylvain. Inserm; Francia. Universite Joseph Fourier; Francia. European Synchrotron Radiation Facility; Francia
Fil: Barbier, Emmanuel. Inserm; Francia. Universite Joseph Fourier; Francia
Materia
NEURONAL CULTURE
PROTOCOL
CYTOTOXICITY
MANGANESE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/185892
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/185892
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Manganese Cytotoxicity Assay on Hippocampal Neuronal Cell CultureDaoust, AlexiaSaoudi, YasminaBrocard, JacquesCollomb, NoraBatandier, CecileBisbal, MarianoSalome, MurielleAndrieux, AnnieBohic, SylvainBarbier, EmmanuelNEURONAL CULTUREPROTOCOLCYTOTOXICITYMANGANESEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Compared to an in vivo experiment, neuronal cell cultures are immediately accessible<br />to observation and manipulation. In this protocol, we describe a technique to evaluate the<br />cytotoxicity of a metal, manganese (Mn2+), on hippocampal neuronal cell cultures. Interestingly, this protocol is easily adaptable to any type of primary culture (e.g., cortical neurons) and any type of toxic compound (e.g., chemical product).Fil: Daoust, Alexia. Inserm; Francia. Universite Joseph Fourier; FranciaFil: Saoudi, Yasmina. Inserm; Francia. Universite Joseph Fourier; FranciaFil: Brocard, Jacques. Inserm; Francia. Universite Joseph Fourier; FranciaFil: Collomb, Nora. Inserm; Francia. Universite Joseph Fourier; FranciaFil: Batandier, Cecile. Laboratoire de Bioénergétique Fondamentale et Appliquée; FranciaFil: Bisbal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Inserm; Francia. Universite Joseph Fourier; FranciaFil: Salome, Murielle. European Synchrotron Radiation Facility; FranciaFil: Andrieux, Annie. Inserm; Francia. Universite Joseph Fourier; FranciaFil: Bohic, Sylvain. Inserm; Francia. Universite Joseph Fourier; Francia. European Synchrotron Radiation Facility; FranciaFil: Barbier, Emmanuel. Inserm; Francia. Universite Joseph Fourier; FranciaBio-protocol LLC2015-01-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/185892Daoust, Alexia; Saoudi, Yasmina; Brocard, Jacques; Collomb, Nora; Batandier, Cecile; et al.; Manganese Cytotoxicity Assay on Hippocampal Neuronal Cell Culture; Bio-protocol LLC; Bio-protocol; 5; 1; 5-1-2015; 1-62331-8325CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.bio-protocol.org/e1368info:eu-repo/semantics/altIdentifier/doi/10.21769/BioProtoc.1368info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:39Zoai:ri.conicet.gov.ar:11336/185892instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:39.458CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Manganese Cytotoxicity Assay on Hippocampal Neuronal Cell Culture
title Manganese Cytotoxicity Assay on Hippocampal Neuronal Cell Culture
spellingShingle Manganese Cytotoxicity Assay on Hippocampal Neuronal Cell Culture
Daoust, Alexia
NEURONAL CULTURE
PROTOCOL
CYTOTOXICITY
MANGANESE
title_short Manganese Cytotoxicity Assay on Hippocampal Neuronal Cell Culture
title_full Manganese Cytotoxicity Assay on Hippocampal Neuronal Cell Culture
title_fullStr Manganese Cytotoxicity Assay on Hippocampal Neuronal Cell Culture
title_full_unstemmed Manganese Cytotoxicity Assay on Hippocampal Neuronal Cell Culture
title_sort Manganese Cytotoxicity Assay on Hippocampal Neuronal Cell Culture
dc.creator.none.fl_str_mv Daoust, Alexia
Saoudi, Yasmina
Brocard, Jacques
Collomb, Nora
Batandier, Cecile
Bisbal, Mariano
Salome, Murielle
Andrieux, Annie
Bohic, Sylvain
Barbier, Emmanuel
author Daoust, Alexia
author_facet Daoust, Alexia
Saoudi, Yasmina
Brocard, Jacques
Collomb, Nora
Batandier, Cecile
Bisbal, Mariano
Salome, Murielle
Andrieux, Annie
Bohic, Sylvain
Barbier, Emmanuel
author_role author
author2 Saoudi, Yasmina
Brocard, Jacques
Collomb, Nora
Batandier, Cecile
Bisbal, Mariano
Salome, Murielle
Andrieux, Annie
Bohic, Sylvain
Barbier, Emmanuel
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv NEURONAL CULTURE
PROTOCOL
CYTOTOXICITY
MANGANESE
topic NEURONAL CULTURE
PROTOCOL
CYTOTOXICITY
MANGANESE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Compared to an in vivo experiment, neuronal cell cultures are immediately accessible<br />to observation and manipulation. In this protocol, we describe a technique to evaluate the<br />cytotoxicity of a metal, manganese (Mn2+), on hippocampal neuronal cell cultures. Interestingly, this protocol is easily adaptable to any type of primary culture (e.g., cortical neurons) and any type of toxic compound (e.g., chemical product).
Fil: Daoust, Alexia. Inserm; Francia. Universite Joseph Fourier; Francia
Fil: Saoudi, Yasmina. Inserm; Francia. Universite Joseph Fourier; Francia
Fil: Brocard, Jacques. Inserm; Francia. Universite Joseph Fourier; Francia
Fil: Collomb, Nora. Inserm; Francia. Universite Joseph Fourier; Francia
Fil: Batandier, Cecile. Laboratoire de Bioénergétique Fondamentale et Appliquée; Francia
Fil: Bisbal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Inserm; Francia. Universite Joseph Fourier; Francia
Fil: Salome, Murielle. European Synchrotron Radiation Facility; Francia
Fil: Andrieux, Annie. Inserm; Francia. Universite Joseph Fourier; Francia
Fil: Bohic, Sylvain. Inserm; Francia. Universite Joseph Fourier; Francia. European Synchrotron Radiation Facility; Francia
Fil: Barbier, Emmanuel. Inserm; Francia. Universite Joseph Fourier; Francia
description Compared to an in vivo experiment, neuronal cell cultures are immediately accessible<br />to observation and manipulation. In this protocol, we describe a technique to evaluate the<br />cytotoxicity of a metal, manganese (Mn2+), on hippocampal neuronal cell cultures. Interestingly, this protocol is easily adaptable to any type of primary culture (e.g., cortical neurons) and any type of toxic compound (e.g., chemical product).
publishDate 2015
dc.date.none.fl_str_mv 2015-01-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/185892
Daoust, Alexia; Saoudi, Yasmina; Brocard, Jacques; Collomb, Nora; Batandier, Cecile; et al.; Manganese Cytotoxicity Assay on Hippocampal Neuronal Cell Culture; Bio-protocol LLC; Bio-protocol; 5; 1; 5-1-2015; 1-6
2331-8325
CONICET Digital
CONICET
url http://hdl.handle.net/11336/185892
identifier_str_mv Daoust, Alexia; Saoudi, Yasmina; Brocard, Jacques; Collomb, Nora; Batandier, Cecile; et al.; Manganese Cytotoxicity Assay on Hippocampal Neuronal Cell Culture; Bio-protocol LLC; Bio-protocol; 5; 1; 5-1-2015; 1-6
2331-8325
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.bio-protocol.org/e1368
info:eu-repo/semantics/altIdentifier/doi/10.21769/BioProtoc.1368
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bio-protocol LLC
publisher.none.fl_str_mv Bio-protocol LLC
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613746513674240
score 13.070432

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