Cytotoxicity of a vanadyl(IV) complex with a multidentate oxygen donor in osteoblast cell lines in culture

Autores
Rivadeneira, Josefina; Di Virgilio, Ana Laura; Barrio, Daniel Alejandro; Muglia, Cecilia Isabel; Bruzzone, L.; Etcheverry, Susana Beatriz
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Strong chelating ligands as oxodiacetate (oda) are model systems to study the process of metal trapping by livingorganisms. Vanadium compounds display interesting biological and pharmacological actions. In vertebrates, vanadiumis stored mainly in bones. In the present study we report the effects of the complex of oda with vanadyl(IV) cation,VO(oda), on two osteoblast cell lines, one normal (MC3T3-E1) and the other tumoral (UMR106). VO(oda) exerted cytotoxicactions in osteoblasts as it was determined through a dose-dependent decrease in cell proliferation, and morphologicaland actin alterations. The putative mechanisms underlying VO(oda) deleterious effects were also investigated. Thecomplex increased the level of ROS which correlated with a decreased in GSH/GSSG ratio. Besides, VO(oda) induced adissipation of the mitochondria membrane potential (MMP) and promoted an increase in ERK cascade phosphorylation,which is involved in the regulation of cellular death and survival. All the effects were more pronounced in MC3T3-E1than in UMR106 cells. ERK activation was inhibited by PD98059, Wortmanin and the ROS scavenger NAC (N-acetylcysteine). These results suggest that VO(oda) stimulated ERKs phosphorylation by induction of free radicals involvingkinases upstream of ERK pathway. The inhibitory effect of the complex on cell proliferation was partially reversed inboth cell lines by NAC. Moreover, PD98059 and Wortmanin also partially reversed the inhibition of cell proliferation inthe tumoral osteoblasts. The use of specific inhibitors and ROS scavengers suggested the involvement of oxidative stress,MMP alterations and ERK pathway in the apoptotic actions of this complex.
Fil: Rivadeneira, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patológica; Argentina
Fil: Di Virgilio, Ana Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Barrio, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patológica; Argentina
Fil: Muglia, Cecilia Isabel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Bruzzone, L.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Etcheverry, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patológica; Argentina
Materia
VANADYL(IV)
MULTIDENTATE LIGAND
CYTOTOXICITY
OSTEOBLASTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/109230
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/109230
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cytotoxicity of a vanadyl(IV) complex with a multidentate oxygen donor in osteoblast cell lines in cultureRivadeneira, JosefinaDi Virgilio, Ana LauraBarrio, Daniel AlejandroMuglia, Cecilia IsabelBruzzone, L.Etcheverry, Susana BeatrizVANADYL(IV)MULTIDENTATE LIGANDCYTOTOXICITYOSTEOBLASTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Strong chelating ligands as oxodiacetate (oda) are model systems to study the process of metal trapping by livingorganisms. Vanadium compounds display interesting biological and pharmacological actions. In vertebrates, vanadiumis stored mainly in bones. In the present study we report the effects of the complex of oda with vanadyl(IV) cation,VO(oda), on two osteoblast cell lines, one normal (MC3T3-E1) and the other tumoral (UMR106). VO(oda) exerted cytotoxicactions in osteoblasts as it was determined through a dose-dependent decrease in cell proliferation, and morphologicaland actin alterations. The putative mechanisms underlying VO(oda) deleterious effects were also investigated. Thecomplex increased the level of ROS which correlated with a decreased in GSH/GSSG ratio. Besides, VO(oda) induced adissipation of the mitochondria membrane potential (MMP) and promoted an increase in ERK cascade phosphorylation,which is involved in the regulation of cellular death and survival. All the effects were more pronounced in MC3T3-E1than in UMR106 cells. ERK activation was inhibited by PD98059, Wortmanin and the ROS scavenger NAC (N-acetylcysteine). These results suggest that VO(oda) stimulated ERKs phosphorylation by induction of free radicals involvingkinases upstream of ERK pathway. The inhibitory effect of the complex on cell proliferation was partially reversed inboth cell lines by NAC. Moreover, PD98059 and Wortmanin also partially reversed the inhibition of cell proliferation inthe tumoral osteoblasts. The use of specific inhibitors and ROS scavengers suggested the involvement of oxidative stress,MMP alterations and ERK pathway in the apoptotic actions of this complex.Fil: Rivadeneira, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patológica; ArgentinaFil: Di Virgilio, Ana Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Barrio, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patológica; ArgentinaFil: Muglia, Cecilia Isabel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Bruzzone, L.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Etcheverry, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patológica; ArgentinaBentham Science Publishers2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/109230Rivadeneira, Josefina; Di Virgilio, Ana Laura; Barrio, Daniel Alejandro; Muglia, Cecilia Isabel; Bruzzone, L.; et al.; Cytotoxicity of a vanadyl(IV) complex with a multidentate oxygen donor in osteoblast cell lines in culture; Bentham Science Publishers; Medicinal Chemistry; 6; 1; 4-2010; 9-231573-4064CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.eurekaselect.com/85443/articleinfo:eu-repo/semantics/altIdentifier/doi/10.2174/157340610791208754info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:33Zoai:ri.conicet.gov.ar:11336/109230instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:33.455CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cytotoxicity of a vanadyl(IV) complex with a multidentate oxygen donor in osteoblast cell lines in culture
title Cytotoxicity of a vanadyl(IV) complex with a multidentate oxygen donor in osteoblast cell lines in culture
spellingShingle Cytotoxicity of a vanadyl(IV) complex with a multidentate oxygen donor in osteoblast cell lines in culture
Rivadeneira, Josefina
VANADYL(IV)
MULTIDENTATE LIGAND
CYTOTOXICITY
OSTEOBLASTS
title_short Cytotoxicity of a vanadyl(IV) complex with a multidentate oxygen donor in osteoblast cell lines in culture
title_full Cytotoxicity of a vanadyl(IV) complex with a multidentate oxygen donor in osteoblast cell lines in culture
title_fullStr Cytotoxicity of a vanadyl(IV) complex with a multidentate oxygen donor in osteoblast cell lines in culture
title_full_unstemmed Cytotoxicity of a vanadyl(IV) complex with a multidentate oxygen donor in osteoblast cell lines in culture
title_sort Cytotoxicity of a vanadyl(IV) complex with a multidentate oxygen donor in osteoblast cell lines in culture
dc.creator.none.fl_str_mv Rivadeneira, Josefina
Di Virgilio, Ana Laura
Barrio, Daniel Alejandro
Muglia, Cecilia Isabel
Bruzzone, L.
Etcheverry, Susana Beatriz
author Rivadeneira, Josefina
author_facet Rivadeneira, Josefina
Di Virgilio, Ana Laura
Barrio, Daniel Alejandro
Muglia, Cecilia Isabel
Bruzzone, L.
Etcheverry, Susana Beatriz
author_role author
author2 Di Virgilio, Ana Laura
Barrio, Daniel Alejandro
Muglia, Cecilia Isabel
Bruzzone, L.
Etcheverry, Susana Beatriz
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv VANADYL(IV)
MULTIDENTATE LIGAND
CYTOTOXICITY
OSTEOBLASTS
topic VANADYL(IV)
MULTIDENTATE LIGAND
CYTOTOXICITY
OSTEOBLASTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Strong chelating ligands as oxodiacetate (oda) are model systems to study the process of metal trapping by livingorganisms. Vanadium compounds display interesting biological and pharmacological actions. In vertebrates, vanadiumis stored mainly in bones. In the present study we report the effects of the complex of oda with vanadyl(IV) cation,VO(oda), on two osteoblast cell lines, one normal (MC3T3-E1) and the other tumoral (UMR106). VO(oda) exerted cytotoxicactions in osteoblasts as it was determined through a dose-dependent decrease in cell proliferation, and morphologicaland actin alterations. The putative mechanisms underlying VO(oda) deleterious effects were also investigated. Thecomplex increased the level of ROS which correlated with a decreased in GSH/GSSG ratio. Besides, VO(oda) induced adissipation of the mitochondria membrane potential (MMP) and promoted an increase in ERK cascade phosphorylation,which is involved in the regulation of cellular death and survival. All the effects were more pronounced in MC3T3-E1than in UMR106 cells. ERK activation was inhibited by PD98059, Wortmanin and the ROS scavenger NAC (N-acetylcysteine). These results suggest that VO(oda) stimulated ERKs phosphorylation by induction of free radicals involvingkinases upstream of ERK pathway. The inhibitory effect of the complex on cell proliferation was partially reversed inboth cell lines by NAC. Moreover, PD98059 and Wortmanin also partially reversed the inhibition of cell proliferation inthe tumoral osteoblasts. The use of specific inhibitors and ROS scavengers suggested the involvement of oxidative stress,MMP alterations and ERK pathway in the apoptotic actions of this complex.
Fil: Rivadeneira, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patológica; Argentina
Fil: Di Virgilio, Ana Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Barrio, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patológica; Argentina
Fil: Muglia, Cecilia Isabel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Bruzzone, L.. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Etcheverry, Susana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra Bioquímica Patológica; Argentina
description Strong chelating ligands as oxodiacetate (oda) are model systems to study the process of metal trapping by livingorganisms. Vanadium compounds display interesting biological and pharmacological actions. In vertebrates, vanadiumis stored mainly in bones. In the present study we report the effects of the complex of oda with vanadyl(IV) cation,VO(oda), on two osteoblast cell lines, one normal (MC3T3-E1) and the other tumoral (UMR106). VO(oda) exerted cytotoxicactions in osteoblasts as it was determined through a dose-dependent decrease in cell proliferation, and morphologicaland actin alterations. The putative mechanisms underlying VO(oda) deleterious effects were also investigated. Thecomplex increased the level of ROS which correlated with a decreased in GSH/GSSG ratio. Besides, VO(oda) induced adissipation of the mitochondria membrane potential (MMP) and promoted an increase in ERK cascade phosphorylation,which is involved in the regulation of cellular death and survival. All the effects were more pronounced in MC3T3-E1than in UMR106 cells. ERK activation was inhibited by PD98059, Wortmanin and the ROS scavenger NAC (N-acetylcysteine). These results suggest that VO(oda) stimulated ERKs phosphorylation by induction of free radicals involvingkinases upstream of ERK pathway. The inhibitory effect of the complex on cell proliferation was partially reversed inboth cell lines by NAC. Moreover, PD98059 and Wortmanin also partially reversed the inhibition of cell proliferation inthe tumoral osteoblasts. The use of specific inhibitors and ROS scavengers suggested the involvement of oxidative stress,MMP alterations and ERK pathway in the apoptotic actions of this complex.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/109230
Rivadeneira, Josefina; Di Virgilio, Ana Laura; Barrio, Daniel Alejandro; Muglia, Cecilia Isabel; Bruzzone, L.; et al.; Cytotoxicity of a vanadyl(IV) complex with a multidentate oxygen donor in osteoblast cell lines in culture; Bentham Science Publishers; Medicinal Chemistry; 6; 1; 4-2010; 9-23
1573-4064
CONICET Digital
CONICET
url http://hdl.handle.net/11336/109230
identifier_str_mv Rivadeneira, Josefina; Di Virgilio, Ana Laura; Barrio, Daniel Alejandro; Muglia, Cecilia Isabel; Bruzzone, L.; et al.; Cytotoxicity of a vanadyl(IV) complex with a multidentate oxygen donor in osteoblast cell lines in culture; Bentham Science Publishers; Medicinal Chemistry; 6; 1; 4-2010; 9-23
1573-4064
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.eurekaselect.com/85443/article
info:eu-repo/semantics/altIdentifier/doi/10.2174/157340610791208754
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bentham Science Publishers
publisher.none.fl_str_mv Bentham Science Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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