Impact of manganese on primary hippocampal neurons from rodents

Autores
Daoust, Alexia; Saoudi, Yasmina; Brocard, Jacques; Collomb, Nora; Batandier, Cecile; Bisbal, Mariano; Salomé, Murielle; Andrieux, Annie; Bohic, Sylvain; Barbier , Emmanuel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Manganese-enhanced magnetic resonance imaging (MEMRI) is a powerful tool for in vivo tract tracing or functional imaging of the central nervous system. However Mn2+ may be toxic at high levels. In this study, we addressed the impact of Mn2+ on mouse hippocampal neurons (HN) and neuron-like N2a cells in culture, using several approaches. Both HN and N2a cells not exposed to exogenous MnCl2 were shown by synchrotron X-ray fluorescence to contain 5 mg/g Mn. Concentrations of Mn2+ leading to 50% lethality (LC50) after 24 h of incubation were much higher for N2a cells (863 mM) than for HN (90 mM). The distribution of Mn2+ in both cell types exposed to Mn2+ concentrations below LC50 was perinuclear whereas that in cells exposed to concentrations above LC50 was more diffuse, suggesting an overloading of cell storage/detoxification capacity. In addition, Mn2+ had a cell-type and dose-dependent impact on the total amount of intracellular P, Ca, Fe and Zn measured by synchrotron X-ray fluorescence. For HN neurons, immunofluorescence studies revealed that concentrations of Mn2+ below LC50 shortened neuritic length and decreased mitochondria velocity after 24 h of incubation. Similar concentrations of Mn2+ also facilitated the opening of the mitochondrial permeability transition pore in isolated mitochondria from rat brains. The sensitivity of primary HN to Mn2+ demonstrated here supports their use as a relevant model to study Mn2+-induced neurotoxicity.
Fil: Daoust, Alexia. Inserm; Francia. Université Grenoble Alpes. Grenoble; Francia
Fil: Saoudi, Yasmina. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia
Fil: Brocard, Jacques. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia
Fil: Collomb, Nora. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia
Fil: Batandier, Cecile. Laboratoire de Bioénergétique. Grenoble; Francia
Fil: Bisbal, Mariano. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salomé, Murielle. European Synchrotron Radiation Facility. Grenoble; Francia
Fil: Andrieux, Annie. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia
Fil: Bohic, Sylvain. European Synchrotron Radiation Facility. Grenoble; Francia. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia
Fil: Barbier , Emmanuel. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia
Materia
Manganese
Memri
X-Ray Synchrotron
Hippocampalneurons
Mitochondria
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31630
Acceder
id CONICETDig_87ecce395f24fbe9a6c0aa1aebf2edc1
oai_identifier_str oai:ri.conicet.gov.ar:11336/31630
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Impact of manganese on primary hippocampal neurons from rodentsDaoust, AlexiaSaoudi, YasminaBrocard, JacquesCollomb, NoraBatandier, CecileBisbal, MarianoSalomé, MurielleAndrieux, AnnieBohic, SylvainBarbier , EmmanuelManganeseMemriX-Ray SynchrotronHippocampalneuronsMitochondriahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Manganese-enhanced magnetic resonance imaging (MEMRI) is a powerful tool for in vivo tract tracing or functional imaging of the central nervous system. However Mn2+ may be toxic at high levels. In this study, we addressed the impact of Mn2+ on mouse hippocampal neurons (HN) and neuron-like N2a cells in culture, using several approaches. Both HN and N2a cells not exposed to exogenous MnCl2 were shown by synchrotron X-ray fluorescence to contain 5 mg/g Mn. Concentrations of Mn2+ leading to 50% lethality (LC50) after 24 h of incubation were much higher for N2a cells (863 mM) than for HN (90 mM). The distribution of Mn2+ in both cell types exposed to Mn2+ concentrations below LC50 was perinuclear whereas that in cells exposed to concentrations above LC50 was more diffuse, suggesting an overloading of cell storage/detoxification capacity. In addition, Mn2+ had a cell-type and dose-dependent impact on the total amount of intracellular P, Ca, Fe and Zn measured by synchrotron X-ray fluorescence. For HN neurons, immunofluorescence studies revealed that concentrations of Mn2+ below LC50 shortened neuritic length and decreased mitochondria velocity after 24 h of incubation. Similar concentrations of Mn2+ also facilitated the opening of the mitochondrial permeability transition pore in isolated mitochondria from rat brains. The sensitivity of primary HN to Mn2+ demonstrated here supports their use as a relevant model to study Mn2+-induced neurotoxicity.Fil: Daoust, Alexia. Inserm; Francia. Université Grenoble Alpes. Grenoble; FranciaFil: Saoudi, Yasmina. Université Grenoble Alpes. Grenoble; Francia. Inserm; FranciaFil: Brocard, Jacques. Université Grenoble Alpes. Grenoble; Francia. Inserm; FranciaFil: Collomb, Nora. Université Grenoble Alpes. Grenoble; Francia. Inserm; FranciaFil: Batandier, Cecile. Laboratoire de Bioénergétique. Grenoble; FranciaFil: Bisbal, Mariano. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Salomé, Murielle. European Synchrotron Radiation Facility. Grenoble; FranciaFil: Andrieux, Annie. Université Grenoble Alpes. Grenoble; Francia. Inserm; FranciaFil: Bohic, Sylvain. European Synchrotron Radiation Facility. Grenoble; Francia. Université Grenoble Alpes. Grenoble; Francia. Inserm; FranciaFil: Barbier , Emmanuel. Université Grenoble Alpes. Grenoble; Francia. Inserm; FranciaWiley-liss, Div John Wiley & Sons Inc2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31630Barbier , Emmanuel; Andrieux, Annie; Salomé, Murielle; Bisbal, Mariano; Batandier, Cecile; Collomb, Nora; et al.; Impact of manganese on primary hippocampal neurons from rodents; Wiley-liss, Div John Wiley & Sons Inc; Hippocampus; 24; 5; 5-2014; 598-6101050-9631CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/hipo.22252info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/hipo.22252/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:38:31Zoai:ri.conicet.gov.ar:11336/31630instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:38:31.723CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Impact of manganese on primary hippocampal neurons from rodents
title Impact of manganese on primary hippocampal neurons from rodents
spellingShingle Impact of manganese on primary hippocampal neurons from rodents
Daoust, Alexia
Manganese
Memri
X-Ray Synchrotron
Hippocampalneurons
Mitochondria
title_short Impact of manganese on primary hippocampal neurons from rodents
title_full Impact of manganese on primary hippocampal neurons from rodents
title_fullStr Impact of manganese on primary hippocampal neurons from rodents
title_full_unstemmed Impact of manganese on primary hippocampal neurons from rodents
title_sort Impact of manganese on primary hippocampal neurons from rodents
dc.creator.none.fl_str_mv Daoust, Alexia
Saoudi, Yasmina
Brocard, Jacques
Collomb, Nora
Batandier, Cecile
Bisbal, Mariano
Salomé, Murielle
Andrieux, Annie
Bohic, Sylvain
Barbier , Emmanuel
author Daoust, Alexia
author_facet Daoust, Alexia
Saoudi, Yasmina
Brocard, Jacques
Collomb, Nora
Batandier, Cecile
Bisbal, Mariano
Salomé, Murielle
Andrieux, Annie
Bohic, Sylvain
Barbier , Emmanuel
author_role author
author2 Saoudi, Yasmina
Brocard, Jacques
Collomb, Nora
Batandier, Cecile
Bisbal, Mariano
Salomé, Murielle
Andrieux, Annie
Bohic, Sylvain
Barbier , Emmanuel
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Manganese
Memri
X-Ray Synchrotron
Hippocampalneurons
Mitochondria
topic Manganese
Memri
X-Ray Synchrotron
Hippocampalneurons
Mitochondria
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Manganese-enhanced magnetic resonance imaging (MEMRI) is a powerful tool for in vivo tract tracing or functional imaging of the central nervous system. However Mn2+ may be toxic at high levels. In this study, we addressed the impact of Mn2+ on mouse hippocampal neurons (HN) and neuron-like N2a cells in culture, using several approaches. Both HN and N2a cells not exposed to exogenous MnCl2 were shown by synchrotron X-ray fluorescence to contain 5 mg/g Mn. Concentrations of Mn2+ leading to 50% lethality (LC50) after 24 h of incubation were much higher for N2a cells (863 mM) than for HN (90 mM). The distribution of Mn2+ in both cell types exposed to Mn2+ concentrations below LC50 was perinuclear whereas that in cells exposed to concentrations above LC50 was more diffuse, suggesting an overloading of cell storage/detoxification capacity. In addition, Mn2+ had a cell-type and dose-dependent impact on the total amount of intracellular P, Ca, Fe and Zn measured by synchrotron X-ray fluorescence. For HN neurons, immunofluorescence studies revealed that concentrations of Mn2+ below LC50 shortened neuritic length and decreased mitochondria velocity after 24 h of incubation. Similar concentrations of Mn2+ also facilitated the opening of the mitochondrial permeability transition pore in isolated mitochondria from rat brains. The sensitivity of primary HN to Mn2+ demonstrated here supports their use as a relevant model to study Mn2+-induced neurotoxicity.
Fil: Daoust, Alexia. Inserm; Francia. Université Grenoble Alpes. Grenoble; Francia
Fil: Saoudi, Yasmina. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia
Fil: Brocard, Jacques. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia
Fil: Collomb, Nora. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia
Fil: Batandier, Cecile. Laboratoire de Bioénergétique. Grenoble; Francia
Fil: Bisbal, Mariano. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salomé, Murielle. European Synchrotron Radiation Facility. Grenoble; Francia
Fil: Andrieux, Annie. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia
Fil: Bohic, Sylvain. European Synchrotron Radiation Facility. Grenoble; Francia. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia
Fil: Barbier , Emmanuel. Université Grenoble Alpes. Grenoble; Francia. Inserm; Francia
description Manganese-enhanced magnetic resonance imaging (MEMRI) is a powerful tool for in vivo tract tracing or functional imaging of the central nervous system. However Mn2+ may be toxic at high levels. In this study, we addressed the impact of Mn2+ on mouse hippocampal neurons (HN) and neuron-like N2a cells in culture, using several approaches. Both HN and N2a cells not exposed to exogenous MnCl2 were shown by synchrotron X-ray fluorescence to contain 5 mg/g Mn. Concentrations of Mn2+ leading to 50% lethality (LC50) after 24 h of incubation were much higher for N2a cells (863 mM) than for HN (90 mM). The distribution of Mn2+ in both cell types exposed to Mn2+ concentrations below LC50 was perinuclear whereas that in cells exposed to concentrations above LC50 was more diffuse, suggesting an overloading of cell storage/detoxification capacity. In addition, Mn2+ had a cell-type and dose-dependent impact on the total amount of intracellular P, Ca, Fe and Zn measured by synchrotron X-ray fluorescence. For HN neurons, immunofluorescence studies revealed that concentrations of Mn2+ below LC50 shortened neuritic length and decreased mitochondria velocity after 24 h of incubation. Similar concentrations of Mn2+ also facilitated the opening of the mitochondrial permeability transition pore in isolated mitochondria from rat brains. The sensitivity of primary HN to Mn2+ demonstrated here supports their use as a relevant model to study Mn2+-induced neurotoxicity.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31630
Barbier , Emmanuel; Andrieux, Annie; Salomé, Murielle; Bisbal, Mariano; Batandier, Cecile; Collomb, Nora; et al.; Impact of manganese on primary hippocampal neurons from rodents; Wiley-liss, Div John Wiley & Sons Inc; Hippocampus; 24; 5; 5-2014; 598-610
1050-9631
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31630
identifier_str_mv Barbier , Emmanuel; Andrieux, Annie; Salomé, Murielle; Bisbal, Mariano; Batandier, Cecile; Collomb, Nora; et al.; Impact of manganese on primary hippocampal neurons from rodents; Wiley-liss, Div John Wiley & Sons Inc; Hippocampus; 24; 5; 5-2014; 598-610
1050-9631
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/hipo.22252
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/hipo.22252/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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