Characterization and subtyping of Listeria monocytogenes strains from butcher shops

Autores
Londero, Alejandra; Costa, Magdalena; Galli, Lucía; Brusa, Victoria; Linares, Luciano; Prieto, Mónica Raquel; Leotta, Gerardo Anibal
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We characterized L. monocytogenes strains isolated from ground beef (n = 40) and butcher shop environmental (n = 99) samples before and after implementing improvement actions. Strains were serotyped and subtyped by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and ApaI-pulsed-field gel electrophoresis (PFGE) to establish their epidemiological relationships, clarify the contamination dynamics and assess the impact of improvement measures. Serotype distribution was as follows: 1/2c (57.6%), 1/2b (18.7%), 4b (12.9%), 1/2a (6.5%) and 4a/4c (4.3%). Thirteen clones were simultaneously isolated in different butcher shops; 73.5% of establishments shared the same beef supplier. Cross-contamination was detected in L. monocytogenes-positive samples from 20% of butcher shops, mostly at the evaluation stage (91.7%). Up to five strains were isolated from each butcher shop at the evaluation (37%) and verification (22%) stages. Seven persistent clones were isolated over the three-year study period. Serotypes 4b and 1/2b significantly decreased during the evaluation stage. Although part of the contamination with L. monocytogenes was introduced by the beef supplier, the pathogen spread profusely in butcher shops, suggesting the relevance of keeping good hygiene control and management of the environment to prevent the spread of L. monocytogenes in butcher shops.
Fil: Londero, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Brusa, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Linares, Luciano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Prieto, Mónica Raquel. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Materia
BUTCHER SHOP
ERIC-PCR
GROUND BEEF
LISTERIA MONOCYTOGENES
PFGE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/119958
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization and subtyping of Listeria monocytogenes strains from butcher shopsLondero, AlejandraCosta, MagdalenaGalli, LucíaBrusa, VictoriaLinares, LucianoPrieto, Mónica RaquelLeotta, Gerardo AnibalBUTCHER SHOPERIC-PCRGROUND BEEFLISTERIA MONOCYTOGENESPFGEhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4We characterized L. monocytogenes strains isolated from ground beef (n = 40) and butcher shop environmental (n = 99) samples before and after implementing improvement actions. Strains were serotyped and subtyped by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and ApaI-pulsed-field gel electrophoresis (PFGE) to establish their epidemiological relationships, clarify the contamination dynamics and assess the impact of improvement measures. Serotype distribution was as follows: 1/2c (57.6%), 1/2b (18.7%), 4b (12.9%), 1/2a (6.5%) and 4a/4c (4.3%). Thirteen clones were simultaneously isolated in different butcher shops; 73.5% of establishments shared the same beef supplier. Cross-contamination was detected in L. monocytogenes-positive samples from 20% of butcher shops, mostly at the evaluation stage (91.7%). Up to five strains were isolated from each butcher shop at the evaluation (37%) and verification (22%) stages. Seven persistent clones were isolated over the three-year study period. Serotypes 4b and 1/2b significantly decreased during the evaluation stage. Although part of the contamination with L. monocytogenes was introduced by the beef supplier, the pathogen spread profusely in butcher shops, suggesting the relevance of keeping good hygiene control and management of the environment to prevent the spread of L. monocytogenes in butcher shops.Fil: Londero, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Brusa, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Linares, Luciano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Prieto, Mónica Raquel. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaElsevier Science2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/119958Londero, Alejandra; Costa, Magdalena; Galli, Lucía; Brusa, Victoria; Linares, Luciano; et al.; Characterization and subtyping of Listeria monocytogenes strains from butcher shops; Elsevier Science; LWT - Food Science and Technology; 113; 10-2019; 1-60023-6438CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0023643819307054info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2019.108363info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:50Zoai:ri.conicet.gov.ar:11336/119958instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:51.27CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization and subtyping of Listeria monocytogenes strains from butcher shops
title Characterization and subtyping of Listeria monocytogenes strains from butcher shops
spellingShingle Characterization and subtyping of Listeria monocytogenes strains from butcher shops
Londero, Alejandra
BUTCHER SHOP
ERIC-PCR
GROUND BEEF
LISTERIA MONOCYTOGENES
PFGE
title_short Characterization and subtyping of Listeria monocytogenes strains from butcher shops
title_full Characterization and subtyping of Listeria monocytogenes strains from butcher shops
title_fullStr Characterization and subtyping of Listeria monocytogenes strains from butcher shops
title_full_unstemmed Characterization and subtyping of Listeria monocytogenes strains from butcher shops
title_sort Characterization and subtyping of Listeria monocytogenes strains from butcher shops
dc.creator.none.fl_str_mv Londero, Alejandra
Costa, Magdalena
Galli, Lucía
Brusa, Victoria
Linares, Luciano
Prieto, Mónica Raquel
Leotta, Gerardo Anibal
author Londero, Alejandra
author_facet Londero, Alejandra
Costa, Magdalena
Galli, Lucía
Brusa, Victoria
Linares, Luciano
Prieto, Mónica Raquel
Leotta, Gerardo Anibal
author_role author
author2 Costa, Magdalena
Galli, Lucía
Brusa, Victoria
Linares, Luciano
Prieto, Mónica Raquel
Leotta, Gerardo Anibal
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BUTCHER SHOP
ERIC-PCR
GROUND BEEF
LISTERIA MONOCYTOGENES
PFGE
topic BUTCHER SHOP
ERIC-PCR
GROUND BEEF
LISTERIA MONOCYTOGENES
PFGE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv We characterized L. monocytogenes strains isolated from ground beef (n = 40) and butcher shop environmental (n = 99) samples before and after implementing improvement actions. Strains were serotyped and subtyped by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and ApaI-pulsed-field gel electrophoresis (PFGE) to establish their epidemiological relationships, clarify the contamination dynamics and assess the impact of improvement measures. Serotype distribution was as follows: 1/2c (57.6%), 1/2b (18.7%), 4b (12.9%), 1/2a (6.5%) and 4a/4c (4.3%). Thirteen clones were simultaneously isolated in different butcher shops; 73.5% of establishments shared the same beef supplier. Cross-contamination was detected in L. monocytogenes-positive samples from 20% of butcher shops, mostly at the evaluation stage (91.7%). Up to five strains were isolated from each butcher shop at the evaluation (37%) and verification (22%) stages. Seven persistent clones were isolated over the three-year study period. Serotypes 4b and 1/2b significantly decreased during the evaluation stage. Although part of the contamination with L. monocytogenes was introduced by the beef supplier, the pathogen spread profusely in butcher shops, suggesting the relevance of keeping good hygiene control and management of the environment to prevent the spread of L. monocytogenes in butcher shops.
Fil: Londero, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Costa, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Galli, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Brusa, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Linares, Luciano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Prieto, Mónica Raquel. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
description We characterized L. monocytogenes strains isolated from ground beef (n = 40) and butcher shop environmental (n = 99) samples before and after implementing improvement actions. Strains were serotyped and subtyped by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and ApaI-pulsed-field gel electrophoresis (PFGE) to establish their epidemiological relationships, clarify the contamination dynamics and assess the impact of improvement measures. Serotype distribution was as follows: 1/2c (57.6%), 1/2b (18.7%), 4b (12.9%), 1/2a (6.5%) and 4a/4c (4.3%). Thirteen clones were simultaneously isolated in different butcher shops; 73.5% of establishments shared the same beef supplier. Cross-contamination was detected in L. monocytogenes-positive samples from 20% of butcher shops, mostly at the evaluation stage (91.7%). Up to five strains were isolated from each butcher shop at the evaluation (37%) and verification (22%) stages. Seven persistent clones were isolated over the three-year study period. Serotypes 4b and 1/2b significantly decreased during the evaluation stage. Although part of the contamination with L. monocytogenes was introduced by the beef supplier, the pathogen spread profusely in butcher shops, suggesting the relevance of keeping good hygiene control and management of the environment to prevent the spread of L. monocytogenes in butcher shops.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/119958
Londero, Alejandra; Costa, Magdalena; Galli, Lucía; Brusa, Victoria; Linares, Luciano; et al.; Characterization and subtyping of Listeria monocytogenes strains from butcher shops; Elsevier Science; LWT - Food Science and Technology; 113; 10-2019; 1-6
0023-6438
CONICET Digital
CONICET
url http://hdl.handle.net/11336/119958
identifier_str_mv Londero, Alejandra; Costa, Magdalena; Galli, Lucía; Brusa, Victoria; Linares, Luciano; et al.; Characterization and subtyping of Listeria monocytogenes strains from butcher shops; Elsevier Science; LWT - Food Science and Technology; 113; 10-2019; 1-6
0023-6438
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0023643819307054
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2019.108363
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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