Anaplasma phagocytophilum Inhibits Apoptosis and Promotes Cytoskeleton Rearrangement for Infection of Tick Cells

Autores
Ayllón, Nieves; Villar, Margarita; Busby, Ann T.; Kocan, Katherine M.; Blouin, Edmour F.; Bonzón Kulichenko, Elena; Galindo, Ruth C.; Mangold, Atilio Jose; Alberdi, Pilar; José M. Pérez de la Lastra; Vázquez, Jesús; De la Fuente, José
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Anaplasma phagocytophilum causes human granulocytic anaplasmosis. Infection with this zoonotic pathogen affects gene expression in both the vertebrate host and the tick vector, Ixodes scapularis. Here, we identified new genes, including spectrin alpha chain or alpha-fodrin (CG8) and voltage-dependent anion-selective channel or mitochondrial porin (T2), that are involved in A. phagocytophilum infection/multiplication and the tick cell response to infection. The pathogen downregulated the expression of CG8 in tick salivary glands and T2 in both the gut and salivary glands to inhibit apoptosis as a mechanism to subvert host cell defenses and increase infection. In the gut, the tick response to infection through CG8 upregulation was used by the pathogen to increase infection due to the cytoskeleton rearrangement that is required for pathogen infection. These results increase our understanding of the role of tick genes during A. phagocytophilum infection and multiplication and demonstrate that the pathogen uses similar strategies to establish infection in both vertebrate and invertebrate hosts.
Fil: Ayllón, Nieves. Instituto de Investigación en Recursos Cinegéticos; España
Fil: Villar, Margarita. Instituto de Investigación en Recursos Cinegéticos; España
Fil: Busby, Ann T.. Oklahoma State University; Estados Unidos
Fil: Kocan, Katherine M.. Oklahoma State University; Estados Unidos
Fil: Blouin, Edmour F.. Oklahoma State University; Estados Unidos
Fil: Bonzón Kulichenko, Elena. Centro de Biología Molecular Severo Ochoa; España
Fil: Galindo, Ruth C.. Instituto de Investigación en Recursos Cinegéticos; España. Oklahoma State University; Estados Unidos
Fil: Mangold, Atilio Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Alberdi, Pilar. Instituto de Investigación en Recursos Cinegéticos; España
Fil: José M. Pérez de la Lastra. Instituto de Investigación en Recursos Cinegéticos; España
Fil: Vázquez, Jesús. Centro de Biología Molecular Severo Ochoa; Estados Unidos
Fil: De la Fuente, José. Instituto de Investigación en Recursos Cinegéticos; España. Oklahoma State University; Estados Unidos
Materia
Anaplasma Phagocytophilum
Human Granulocytic Anaplasmosis
Equine Granulocytic Anaplasmosis
Ixodes Scapularis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/26072
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/26072
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Anaplasma phagocytophilum Inhibits Apoptosis and Promotes Cytoskeleton Rearrangement for Infection of Tick CellsAyllón, NievesVillar, MargaritaBusby, Ann T.Kocan, Katherine M.Blouin, Edmour F.Bonzón Kulichenko, ElenaGalindo, Ruth C.Mangold, Atilio JoseAlberdi, PilarJosé M. Pérez de la LastraVázquez, JesúsDe la Fuente, JoséAnaplasma PhagocytophilumHuman Granulocytic AnaplasmosisEquine Granulocytic AnaplasmosisIxodes ScapularisAnaplasma phagocytophilum causes human granulocytic anaplasmosis. Infection with this zoonotic pathogen affects gene expression in both the vertebrate host and the tick vector, Ixodes scapularis. Here, we identified new genes, including spectrin alpha chain or alpha-fodrin (CG8) and voltage-dependent anion-selective channel or mitochondrial porin (T2), that are involved in A. phagocytophilum infection/multiplication and the tick cell response to infection. The pathogen downregulated the expression of CG8 in tick salivary glands and T2 in both the gut and salivary glands to inhibit apoptosis as a mechanism to subvert host cell defenses and increase infection. In the gut, the tick response to infection through CG8 upregulation was used by the pathogen to increase infection due to the cytoskeleton rearrangement that is required for pathogen infection. These results increase our understanding of the role of tick genes during A. phagocytophilum infection and multiplication and demonstrate that the pathogen uses similar strategies to establish infection in both vertebrate and invertebrate hosts.Fil: Ayllón, Nieves. Instituto de Investigación en Recursos Cinegéticos; EspañaFil: Villar, Margarita. Instituto de Investigación en Recursos Cinegéticos; EspañaFil: Busby, Ann T.. Oklahoma State University; Estados UnidosFil: Kocan, Katherine M.. Oklahoma State University; Estados UnidosFil: Blouin, Edmour F.. Oklahoma State University; Estados UnidosFil: Bonzón Kulichenko, Elena. Centro de Biología Molecular Severo Ochoa; EspañaFil: Galindo, Ruth C.. Instituto de Investigación en Recursos Cinegéticos; España. Oklahoma State University; Estados UnidosFil: Mangold, Atilio Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Alberdi, Pilar. Instituto de Investigación en Recursos Cinegéticos; EspañaFil: José M. Pérez de la Lastra. Instituto de Investigación en Recursos Cinegéticos; EspañaFil: Vázquez, Jesús. Centro de Biología Molecular Severo Ochoa; Estados UnidosFil: De la Fuente, José. Instituto de Investigación en Recursos Cinegéticos; España. Oklahoma State University; Estados UnidosAmerican Society for Microbiology2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26072Ayllón, Nieves; Villar, Margarita; Busby, Ann T.; Kocan, Katherine M.; Blouin, Edmour F.; et al.; Anaplasma phagocytophilum Inhibits Apoptosis and Promotes Cytoskeleton Rearrangement for Infection of Tick Cells; American Society for Microbiology; Infection and Immunity; 81; 7; 7-2013; 24150019-9567CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/IAI.00194-13info:eu-repo/semantics/altIdentifier/url/http://iai.asm.org/content/81/7/2415info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:07:04Zoai:ri.conicet.gov.ar:11336/26072instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:07:04.972CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anaplasma phagocytophilum Inhibits Apoptosis and Promotes Cytoskeleton Rearrangement for Infection of Tick Cells
title Anaplasma phagocytophilum Inhibits Apoptosis and Promotes Cytoskeleton Rearrangement for Infection of Tick Cells
spellingShingle Anaplasma phagocytophilum Inhibits Apoptosis and Promotes Cytoskeleton Rearrangement for Infection of Tick Cells
Ayllón, Nieves
Anaplasma Phagocytophilum
Human Granulocytic Anaplasmosis
Equine Granulocytic Anaplasmosis
Ixodes Scapularis
title_short Anaplasma phagocytophilum Inhibits Apoptosis and Promotes Cytoskeleton Rearrangement for Infection of Tick Cells
title_full Anaplasma phagocytophilum Inhibits Apoptosis and Promotes Cytoskeleton Rearrangement for Infection of Tick Cells
title_fullStr Anaplasma phagocytophilum Inhibits Apoptosis and Promotes Cytoskeleton Rearrangement for Infection of Tick Cells
title_full_unstemmed Anaplasma phagocytophilum Inhibits Apoptosis and Promotes Cytoskeleton Rearrangement for Infection of Tick Cells
title_sort Anaplasma phagocytophilum Inhibits Apoptosis and Promotes Cytoskeleton Rearrangement for Infection of Tick Cells
dc.creator.none.fl_str_mv Ayllón, Nieves
Villar, Margarita
Busby, Ann T.
Kocan, Katherine M.
Blouin, Edmour F.
Bonzón Kulichenko, Elena
Galindo, Ruth C.
Mangold, Atilio Jose
Alberdi, Pilar
José M. Pérez de la Lastra
Vázquez, Jesús
De la Fuente, José
author Ayllón, Nieves
author_facet Ayllón, Nieves
Villar, Margarita
Busby, Ann T.
Kocan, Katherine M.
Blouin, Edmour F.
Bonzón Kulichenko, Elena
Galindo, Ruth C.
Mangold, Atilio Jose
Alberdi, Pilar
José M. Pérez de la Lastra
Vázquez, Jesús
De la Fuente, José
author_role author
author2 Villar, Margarita
Busby, Ann T.
Kocan, Katherine M.
Blouin, Edmour F.
Bonzón Kulichenko, Elena
Galindo, Ruth C.
Mangold, Atilio Jose
Alberdi, Pilar
José M. Pérez de la Lastra
Vázquez, Jesús
De la Fuente, José
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Anaplasma Phagocytophilum
Human Granulocytic Anaplasmosis
Equine Granulocytic Anaplasmosis
Ixodes Scapularis
topic Anaplasma Phagocytophilum
Human Granulocytic Anaplasmosis
Equine Granulocytic Anaplasmosis
Ixodes Scapularis
dc.description.none.fl_txt_mv Anaplasma phagocytophilum causes human granulocytic anaplasmosis. Infection with this zoonotic pathogen affects gene expression in both the vertebrate host and the tick vector, Ixodes scapularis. Here, we identified new genes, including spectrin alpha chain or alpha-fodrin (CG8) and voltage-dependent anion-selective channel or mitochondrial porin (T2), that are involved in A. phagocytophilum infection/multiplication and the tick cell response to infection. The pathogen downregulated the expression of CG8 in tick salivary glands and T2 in both the gut and salivary glands to inhibit apoptosis as a mechanism to subvert host cell defenses and increase infection. In the gut, the tick response to infection through CG8 upregulation was used by the pathogen to increase infection due to the cytoskeleton rearrangement that is required for pathogen infection. These results increase our understanding of the role of tick genes during A. phagocytophilum infection and multiplication and demonstrate that the pathogen uses similar strategies to establish infection in both vertebrate and invertebrate hosts.
Fil: Ayllón, Nieves. Instituto de Investigación en Recursos Cinegéticos; España
Fil: Villar, Margarita. Instituto de Investigación en Recursos Cinegéticos; España
Fil: Busby, Ann T.. Oklahoma State University; Estados Unidos
Fil: Kocan, Katherine M.. Oklahoma State University; Estados Unidos
Fil: Blouin, Edmour F.. Oklahoma State University; Estados Unidos
Fil: Bonzón Kulichenko, Elena. Centro de Biología Molecular Severo Ochoa; España
Fil: Galindo, Ruth C.. Instituto de Investigación en Recursos Cinegéticos; España. Oklahoma State University; Estados Unidos
Fil: Mangold, Atilio Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Alberdi, Pilar. Instituto de Investigación en Recursos Cinegéticos; España
Fil: José M. Pérez de la Lastra. Instituto de Investigación en Recursos Cinegéticos; España
Fil: Vázquez, Jesús. Centro de Biología Molecular Severo Ochoa; Estados Unidos
Fil: De la Fuente, José. Instituto de Investigación en Recursos Cinegéticos; España. Oklahoma State University; Estados Unidos
description Anaplasma phagocytophilum causes human granulocytic anaplasmosis. Infection with this zoonotic pathogen affects gene expression in both the vertebrate host and the tick vector, Ixodes scapularis. Here, we identified new genes, including spectrin alpha chain or alpha-fodrin (CG8) and voltage-dependent anion-selective channel or mitochondrial porin (T2), that are involved in A. phagocytophilum infection/multiplication and the tick cell response to infection. The pathogen downregulated the expression of CG8 in tick salivary glands and T2 in both the gut and salivary glands to inhibit apoptosis as a mechanism to subvert host cell defenses and increase infection. In the gut, the tick response to infection through CG8 upregulation was used by the pathogen to increase infection due to the cytoskeleton rearrangement that is required for pathogen infection. These results increase our understanding of the role of tick genes during A. phagocytophilum infection and multiplication and demonstrate that the pathogen uses similar strategies to establish infection in both vertebrate and invertebrate hosts.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/26072
Ayllón, Nieves; Villar, Margarita; Busby, Ann T.; Kocan, Katherine M.; Blouin, Edmour F.; et al.; Anaplasma phagocytophilum Inhibits Apoptosis and Promotes Cytoskeleton Rearrangement for Infection of Tick Cells; American Society for Microbiology; Infection and Immunity; 81; 7; 7-2013; 2415
0019-9567
CONICET Digital
CONICET
url http://hdl.handle.net/11336/26072
identifier_str_mv Ayllón, Nieves; Villar, Margarita; Busby, Ann T.; Kocan, Katherine M.; Blouin, Edmour F.; et al.; Anaplasma phagocytophilum Inhibits Apoptosis and Promotes Cytoskeleton Rearrangement for Infection of Tick Cells; American Society for Microbiology; Infection and Immunity; 81; 7; 7-2013; 2415
0019-9567
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/IAI.00194-13
info:eu-repo/semantics/altIdentifier/url/http://iai.asm.org/content/81/7/2415
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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