Nonylphenol Impairs Steroidogenesis And Lipid Homeostasis During Testicular Development In Mouse Organ Culture
- Autores
- Tajes Ardanaz, Oliverio Julián; Luquez, Jessica Mariela; Sánchez Chaves, Manuela Alejandra; Oresti, Gerardo Martin
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Nonylphenol (NP), a chemical known for its endocrine-disrupting properties, is an environmental pollutant associated with numerous adverse effects on male fertility in both animal models and wild species. In this study, we used a gas-liquid interface testicular culture system to investigate the impact of NP on ex vivo neonatal testicular development. Our objectives were to measure the production levels of testosterone (Tes) and 17β-estradiol (E2), to analyze the expression of genes encoding key proteins involved in the biosynthesis of these hormones, and to assess alterations in testicular lipid composition in NP-exposed ex vivo cultured testes. Our results demonstrated that NP disrupted both the differentiation and maintenance of spermatogenic cells. Furthermore, NP significantly downregulated the transcript levels of marker genes associated with spermatogonial, meiotic, and haploid spermatogenic cells compared to controls. Histological analysis revealed that the seminiferous tubules were depleted of spermatogenic cells, with a concomitant increase in the number of interstitial cells. These observations were consistent with the macroscopic finding that, after 22 days of culture, the tissue size was significantly reduced compared to the control. Notably, NP-exposed tissue produced higher levels of Tes compared to the control during the 0 to 25-day culture period, while E2 levels tended to decrease. This was consistent with an observed increase in the expression of 3β-Hydroxysteroid Dehydrogenase (3β- Hsd1) and a decrease in Cytochrome P450 Aromatase (Cyp19a1), enzymes involved in the production of Tes and E2, respectively. In addition, the explants exposed to NP had higher levels of neutral lipids, particularly triacylglycerols (TAG) and cholesterol esters (CE), compared to those cultured under control conditions. We observed an increase in neutral lipid droplets within both intratubular and interstitial cells, likely containing the accumulated TAG and CE. In summary, this study shows that NP negatively impacts prepubertal spermatogenesis and germ cell maintenance, while also disrupting steroidogenesis and causing imbalances in hormonal and lipid homeostasis.
Fil: Tajes Ardanaz, Oliverio Julián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Luquez, Jessica Mariela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Sánchez Chaves, Manuela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Córdoba
Argentina
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular - Materia
-
ENDOCRINE DISRUPTORS
FATTY ACIDS
TESTIS
GERM CELLS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/278768
Ver los metadatos del registro completo
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Nonylphenol Impairs Steroidogenesis And Lipid Homeostasis During Testicular Development In Mouse Organ CultureTajes Ardanaz, Oliverio JuliánLuquez, Jessica MarielaSánchez Chaves, Manuela AlejandraOresti, Gerardo MartinENDOCRINE DISRUPTORSFATTY ACIDSTESTISGERM CELLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nonylphenol (NP), a chemical known for its endocrine-disrupting properties, is an environmental pollutant associated with numerous adverse effects on male fertility in both animal models and wild species. In this study, we used a gas-liquid interface testicular culture system to investigate the impact of NP on ex vivo neonatal testicular development. Our objectives were to measure the production levels of testosterone (Tes) and 17β-estradiol (E2), to analyze the expression of genes encoding key proteins involved in the biosynthesis of these hormones, and to assess alterations in testicular lipid composition in NP-exposed ex vivo cultured testes. Our results demonstrated that NP disrupted both the differentiation and maintenance of spermatogenic cells. Furthermore, NP significantly downregulated the transcript levels of marker genes associated with spermatogonial, meiotic, and haploid spermatogenic cells compared to controls. Histological analysis revealed that the seminiferous tubules were depleted of spermatogenic cells, with a concomitant increase in the number of interstitial cells. These observations were consistent with the macroscopic finding that, after 22 days of culture, the tissue size was significantly reduced compared to the control. Notably, NP-exposed tissue produced higher levels of Tes compared to the control during the 0 to 25-day culture period, while E2 levels tended to decrease. This was consistent with an observed increase in the expression of 3β-Hydroxysteroid Dehydrogenase (3β- Hsd1) and a decrease in Cytochrome P450 Aromatase (Cyp19a1), enzymes involved in the production of Tes and E2, respectively. In addition, the explants exposed to NP had higher levels of neutral lipids, particularly triacylglycerols (TAG) and cholesterol esters (CE), compared to those cultured under control conditions. We observed an increase in neutral lipid droplets within both intratubular and interstitial cells, likely containing the accumulated TAG and CE. In summary, this study shows that NP negatively impacts prepubertal spermatogenesis and germ cell maintenance, while also disrupting steroidogenesis and causing imbalances in hormonal and lipid homeostasis.Fil: Tajes Ardanaz, Oliverio Julián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Luquez, Jessica Mariela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Sánchez Chaves, Manuela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaLX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology ResearchCórdobaArgentinaSociedad Argentina de Investigaciones en Bioquímica y Biología MolecularSociedad Argentina de Investigaciones en Bioquímica y Biología Molecular2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/278768Nonylphenol Impairs Steroidogenesis And Lipid Homeostasis During Testicular Development In Mouse Organ Culture; LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Córdoba; Argentina; 2024; 101-102CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/2024/abstracts-EN.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-14T12:00:59Zoai:ri.conicet.gov.ar:11336/278768instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-14 12:00:59.711CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Nonylphenol Impairs Steroidogenesis And Lipid Homeostasis During Testicular Development In Mouse Organ Culture |
| title |
Nonylphenol Impairs Steroidogenesis And Lipid Homeostasis During Testicular Development In Mouse Organ Culture |
| spellingShingle |
Nonylphenol Impairs Steroidogenesis And Lipid Homeostasis During Testicular Development In Mouse Organ Culture Tajes Ardanaz, Oliverio Julián ENDOCRINE DISRUPTORS FATTY ACIDS TESTIS GERM CELLS |
| title_short |
Nonylphenol Impairs Steroidogenesis And Lipid Homeostasis During Testicular Development In Mouse Organ Culture |
| title_full |
Nonylphenol Impairs Steroidogenesis And Lipid Homeostasis During Testicular Development In Mouse Organ Culture |
| title_fullStr |
Nonylphenol Impairs Steroidogenesis And Lipid Homeostasis During Testicular Development In Mouse Organ Culture |
| title_full_unstemmed |
Nonylphenol Impairs Steroidogenesis And Lipid Homeostasis During Testicular Development In Mouse Organ Culture |
| title_sort |
Nonylphenol Impairs Steroidogenesis And Lipid Homeostasis During Testicular Development In Mouse Organ Culture |
| dc.creator.none.fl_str_mv |
Tajes Ardanaz, Oliverio Julián Luquez, Jessica Mariela Sánchez Chaves, Manuela Alejandra Oresti, Gerardo Martin |
| author |
Tajes Ardanaz, Oliverio Julián |
| author_facet |
Tajes Ardanaz, Oliverio Julián Luquez, Jessica Mariela Sánchez Chaves, Manuela Alejandra Oresti, Gerardo Martin |
| author_role |
author |
| author2 |
Luquez, Jessica Mariela Sánchez Chaves, Manuela Alejandra Oresti, Gerardo Martin |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
ENDOCRINE DISRUPTORS FATTY ACIDS TESTIS GERM CELLS |
| topic |
ENDOCRINE DISRUPTORS FATTY ACIDS TESTIS GERM CELLS |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
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Nonylphenol (NP), a chemical known for its endocrine-disrupting properties, is an environmental pollutant associated with numerous adverse effects on male fertility in both animal models and wild species. In this study, we used a gas-liquid interface testicular culture system to investigate the impact of NP on ex vivo neonatal testicular development. Our objectives were to measure the production levels of testosterone (Tes) and 17β-estradiol (E2), to analyze the expression of genes encoding key proteins involved in the biosynthesis of these hormones, and to assess alterations in testicular lipid composition in NP-exposed ex vivo cultured testes. Our results demonstrated that NP disrupted both the differentiation and maintenance of spermatogenic cells. Furthermore, NP significantly downregulated the transcript levels of marker genes associated with spermatogonial, meiotic, and haploid spermatogenic cells compared to controls. Histological analysis revealed that the seminiferous tubules were depleted of spermatogenic cells, with a concomitant increase in the number of interstitial cells. These observations were consistent with the macroscopic finding that, after 22 days of culture, the tissue size was significantly reduced compared to the control. Notably, NP-exposed tissue produced higher levels of Tes compared to the control during the 0 to 25-day culture period, while E2 levels tended to decrease. This was consistent with an observed increase in the expression of 3β-Hydroxysteroid Dehydrogenase (3β- Hsd1) and a decrease in Cytochrome P450 Aromatase (Cyp19a1), enzymes involved in the production of Tes and E2, respectively. In addition, the explants exposed to NP had higher levels of neutral lipids, particularly triacylglycerols (TAG) and cholesterol esters (CE), compared to those cultured under control conditions. We observed an increase in neutral lipid droplets within both intratubular and interstitial cells, likely containing the accumulated TAG and CE. In summary, this study shows that NP negatively impacts prepubertal spermatogenesis and germ cell maintenance, while also disrupting steroidogenesis and causing imbalances in hormonal and lipid homeostasis. Fil: Tajes Ardanaz, Oliverio Julián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Luquez, Jessica Mariela. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: Sánchez Chaves, Manuela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research Córdoba Argentina Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular |
| description |
Nonylphenol (NP), a chemical known for its endocrine-disrupting properties, is an environmental pollutant associated with numerous adverse effects on male fertility in both animal models and wild species. In this study, we used a gas-liquid interface testicular culture system to investigate the impact of NP on ex vivo neonatal testicular development. Our objectives were to measure the production levels of testosterone (Tes) and 17β-estradiol (E2), to analyze the expression of genes encoding key proteins involved in the biosynthesis of these hormones, and to assess alterations in testicular lipid composition in NP-exposed ex vivo cultured testes. Our results demonstrated that NP disrupted both the differentiation and maintenance of spermatogenic cells. Furthermore, NP significantly downregulated the transcript levels of marker genes associated with spermatogonial, meiotic, and haploid spermatogenic cells compared to controls. Histological analysis revealed that the seminiferous tubules were depleted of spermatogenic cells, with a concomitant increase in the number of interstitial cells. These observations were consistent with the macroscopic finding that, after 22 days of culture, the tissue size was significantly reduced compared to the control. Notably, NP-exposed tissue produced higher levels of Tes compared to the control during the 0 to 25-day culture period, while E2 levels tended to decrease. This was consistent with an observed increase in the expression of 3β-Hydroxysteroid Dehydrogenase (3β- Hsd1) and a decrease in Cytochrome P450 Aromatase (Cyp19a1), enzymes involved in the production of Tes and E2, respectively. In addition, the explants exposed to NP had higher levels of neutral lipids, particularly triacylglycerols (TAG) and cholesterol esters (CE), compared to those cultured under control conditions. We observed an increase in neutral lipid droplets within both intratubular and interstitial cells, likely containing the accumulated TAG and CE. In summary, this study shows that NP negatively impacts prepubertal spermatogenesis and germ cell maintenance, while also disrupting steroidogenesis and causing imbalances in hormonal and lipid homeostasis. |
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2024 |
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2024 |
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Nonylphenol Impairs Steroidogenesis And Lipid Homeostasis During Testicular Development In Mouse Organ Culture; LX Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Córdoba; Argentina; 2024; 101-102 CONICET Digital CONICET |
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