Nonylphenol Induces Cytoskeletal Changes And Release Of Proinflammatory Mediators In Rat Sertoli Cells In Vitro

Autores
Valles, Ana Sofia; Godoy Sepúlveda, Rodrigo; Oresti, Gerardo Martin; Moreno, Ricardo D.; Aveldaño, Marta Isabel; Reyes, Juan G.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Nonylphenol (NP), an alkylphenol present in plasticizers, is an endocrine disrupting chemical that is potentially dangerous for male reproduction in mammals including man. Because Sertoli cells (SC) provide structural and metabolic support to germ cells, in this study the hypothesis that exposures of SC to NP affect their metabolic functions and the production of bioactive molecules was evaluated. Primary cultures of SC were preincubated with [3 H]arachidonic acid (AA) to label their lipids and then were treated with NP. The NP exposures resulted in increased concentrations of free AA in cells and medium, indicating that such AA was released from [3 H]-labeled lipids (p<0.01). This lipid was mostly phosphatidylinositol, acted upon after activation of a protein kinase A (PKA)/cytoplasmic phospholipase A2 (cPLA2). In NP-exposed SC, an increase of diacylglycerols (DAG) also took place in both, cells and medium (p<0.01). Part of such DAG may have served as second messengers, since NP-increased DAG were associated with an augmented production of PGE2 and expression (mRNA) of COX2. Since the network of vimentin intermediate filaments is important for intracellular lipid transport, the effects of NP on the structure of this network in relation to the formation of cytoplasmic lipid droplets (LD) was studied. In NP-treated SC, the vimentin network was redistributed and the LD size was increased. The NP-dependent cytoskeletal redistribution was prevented by preincubation with H89, a PKA inhibitor. The formation of large LD was prevented by preincubation with either H89 or MEP, a PLA2 inhibitor, suggesting the participation of PKA and cPLA2 in LD biogenesis. We conclude that NP is involved in activating the proinflammatory pathway in SC, by providing the AA that is necessary for prostaglandin synthesis via PKA/PLA2 on the one hand, and by generating the DAG that is required as cofactor of the PKC-mediated activation of the NF- κβ/Cox-2 inflammatory pathway on the other.
Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Godoy Sepúlveda, Rodrigo. Pontificia Universidad Católica de Valparaíso; Chile
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Moreno, Ricardo D.. Pontificia Universidad Católica de Valparaíso; Chile
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Reyes, Juan G.. Pontificia Universidad Católica de Valparaíso; Chile
LXII Reunión Científica de la Sociedad Argentina de Investigación Clínica; LIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica ; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología
Buenos Aires
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Sociedad Argentina de Inmunología
Sociedad Argentina de Andrología
Sociedad Argentina de Biofísica
Sociedad Argentina de Biología
Sociedad Argentina de Farmacología Experimental
Sociedad Argentina de Fisiologia
Sociedad Argentina de Hematología
Sociedad Argentina de Protozoología
Materia
endocrine disruptors
(in)fertility
proinflammatory mediators
COX2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/237017
Acceder
id CONICETDig_3ad9d84c413f9b1476ac46c4a7580b77
oai_identifier_str oai:ri.conicet.gov.ar:11336/237017
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nonylphenol Induces Cytoskeletal Changes And Release Of Proinflammatory Mediators In Rat Sertoli Cells In VitroValles, Ana SofiaGodoy Sepúlveda, RodrigoOresti, Gerardo MartinMoreno, Ricardo D.Aveldaño, Marta IsabelReyes, Juan G.endocrine disruptors(in)fertilityproinflammatory mediatorsCOX2https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nonylphenol (NP), an alkylphenol present in plasticizers, is an endocrine disrupting chemical that is potentially dangerous for male reproduction in mammals including man. Because Sertoli cells (SC) provide structural and metabolic support to germ cells, in this study the hypothesis that exposures of SC to NP affect their metabolic functions and the production of bioactive molecules was evaluated. Primary cultures of SC were preincubated with [3 H]arachidonic acid (AA) to label their lipids and then were treated with NP. The NP exposures resulted in increased concentrations of free AA in cells and medium, indicating that such AA was released from [3 H]-labeled lipids (p<0.01). This lipid was mostly phosphatidylinositol, acted upon after activation of a protein kinase A (PKA)/cytoplasmic phospholipase A2 (cPLA2). In NP-exposed SC, an increase of diacylglycerols (DAG) also took place in both, cells and medium (p<0.01). Part of such DAG may have served as second messengers, since NP-increased DAG were associated with an augmented production of PGE2 and expression (mRNA) of COX2. Since the network of vimentin intermediate filaments is important for intracellular lipid transport, the effects of NP on the structure of this network in relation to the formation of cytoplasmic lipid droplets (LD) was studied. In NP-treated SC, the vimentin network was redistributed and the LD size was increased. The NP-dependent cytoskeletal redistribution was prevented by preincubation with H89, a PKA inhibitor. The formation of large LD was prevented by preincubation with either H89 or MEP, a PLA2 inhibitor, suggesting the participation of PKA and cPLA2 in LD biogenesis. We conclude that NP is involved in activating the proinflammatory pathway in SC, by providing the AA that is necessary for prostaglandin synthesis via PKA/PLA2 on the one hand, and by generating the DAG that is required as cofactor of the PKC-mediated activation of the NF- κβ/Cox-2 inflammatory pathway on the other.Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Godoy Sepúlveda, Rodrigo. Pontificia Universidad Católica de Valparaíso; ChileFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Moreno, Ricardo D.. Pontificia Universidad Católica de Valparaíso; ChileFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Reyes, Juan G.. Pontificia Universidad Católica de Valparaíso; ChileLXII Reunión Científica de la Sociedad Argentina de Investigación Clínica; LIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica ; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de ProtozoologíaBuenos AiresArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de Investigación Bioquímica y Biología MolecularSociedad Argentina de InmunologíaSociedad Argentina de AndrologíaSociedad Argentina de BiofísicaSociedad Argentina de BiologíaSociedad Argentina de Farmacología ExperimentalSociedad Argentina de FisiologiaSociedad Argentina de HematologíaSociedad Argentina de ProtozoologíaFundación Revista Medicina2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/237017Nonylphenol Induces Cytoskeletal Changes And Release Of Proinflammatory Mediators In Rat Sertoli Cells In Vitro; LXII Reunión Científica de la Sociedad Argentina de Investigación Clínica; LIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica ; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología; Buenos Aires; Argentina; 2017; 123-1230025-76801667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-14T12:53:02Zoai:ri.conicet.gov.ar:11336/237017instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-14 12:53:03.045CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nonylphenol Induces Cytoskeletal Changes And Release Of Proinflammatory Mediators In Rat Sertoli Cells In Vitro
title Nonylphenol Induces Cytoskeletal Changes And Release Of Proinflammatory Mediators In Rat Sertoli Cells In Vitro
spellingShingle Nonylphenol Induces Cytoskeletal Changes And Release Of Proinflammatory Mediators In Rat Sertoli Cells In Vitro
Valles, Ana Sofia
endocrine disruptors
(in)fertility
proinflammatory mediators
COX2
title_short Nonylphenol Induces Cytoskeletal Changes And Release Of Proinflammatory Mediators In Rat Sertoli Cells In Vitro
title_full Nonylphenol Induces Cytoskeletal Changes And Release Of Proinflammatory Mediators In Rat Sertoli Cells In Vitro
title_fullStr Nonylphenol Induces Cytoskeletal Changes And Release Of Proinflammatory Mediators In Rat Sertoli Cells In Vitro
title_full_unstemmed Nonylphenol Induces Cytoskeletal Changes And Release Of Proinflammatory Mediators In Rat Sertoli Cells In Vitro
title_sort Nonylphenol Induces Cytoskeletal Changes And Release Of Proinflammatory Mediators In Rat Sertoli Cells In Vitro
dc.creator.none.fl_str_mv Valles, Ana Sofia
Godoy Sepúlveda, Rodrigo
Oresti, Gerardo Martin
Moreno, Ricardo D.
Aveldaño, Marta Isabel
Reyes, Juan G.
author Valles, Ana Sofia
author_facet Valles, Ana Sofia
Godoy Sepúlveda, Rodrigo
Oresti, Gerardo Martin
Moreno, Ricardo D.
Aveldaño, Marta Isabel
Reyes, Juan G.
author_role author
author2 Godoy Sepúlveda, Rodrigo
Oresti, Gerardo Martin
Moreno, Ricardo D.
Aveldaño, Marta Isabel
Reyes, Juan G.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv endocrine disruptors
(in)fertility
proinflammatory mediators
COX2
topic endocrine disruptors
(in)fertility
proinflammatory mediators
COX2
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Nonylphenol (NP), an alkylphenol present in plasticizers, is an endocrine disrupting chemical that is potentially dangerous for male reproduction in mammals including man. Because Sertoli cells (SC) provide structural and metabolic support to germ cells, in this study the hypothesis that exposures of SC to NP affect their metabolic functions and the production of bioactive molecules was evaluated. Primary cultures of SC were preincubated with [3 H]arachidonic acid (AA) to label their lipids and then were treated with NP. The NP exposures resulted in increased concentrations of free AA in cells and medium, indicating that such AA was released from [3 H]-labeled lipids (p<0.01). This lipid was mostly phosphatidylinositol, acted upon after activation of a protein kinase A (PKA)/cytoplasmic phospholipase A2 (cPLA2). In NP-exposed SC, an increase of diacylglycerols (DAG) also took place in both, cells and medium (p<0.01). Part of such DAG may have served as second messengers, since NP-increased DAG were associated with an augmented production of PGE2 and expression (mRNA) of COX2. Since the network of vimentin intermediate filaments is important for intracellular lipid transport, the effects of NP on the structure of this network in relation to the formation of cytoplasmic lipid droplets (LD) was studied. In NP-treated SC, the vimentin network was redistributed and the LD size was increased. The NP-dependent cytoskeletal redistribution was prevented by preincubation with H89, a PKA inhibitor. The formation of large LD was prevented by preincubation with either H89 or MEP, a PLA2 inhibitor, suggesting the participation of PKA and cPLA2 in LD biogenesis. We conclude that NP is involved in activating the proinflammatory pathway in SC, by providing the AA that is necessary for prostaglandin synthesis via PKA/PLA2 on the one hand, and by generating the DAG that is required as cofactor of the PKC-mediated activation of the NF- κβ/Cox-2 inflammatory pathway on the other.
Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Godoy Sepúlveda, Rodrigo. Pontificia Universidad Católica de Valparaíso; Chile
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Moreno, Ricardo D.. Pontificia Universidad Católica de Valparaíso; Chile
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Reyes, Juan G.. Pontificia Universidad Católica de Valparaíso; Chile
LXII Reunión Científica de la Sociedad Argentina de Investigación Clínica; LIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica ; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología
Buenos Aires
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Sociedad Argentina de Inmunología
Sociedad Argentina de Andrología
Sociedad Argentina de Biofísica
Sociedad Argentina de Biología
Sociedad Argentina de Farmacología Experimental
Sociedad Argentina de Fisiologia
Sociedad Argentina de Hematología
Sociedad Argentina de Protozoología
description Nonylphenol (NP), an alkylphenol present in plasticizers, is an endocrine disrupting chemical that is potentially dangerous for male reproduction in mammals including man. Because Sertoli cells (SC) provide structural and metabolic support to germ cells, in this study the hypothesis that exposures of SC to NP affect their metabolic functions and the production of bioactive molecules was evaluated. Primary cultures of SC were preincubated with [3 H]arachidonic acid (AA) to label their lipids and then were treated with NP. The NP exposures resulted in increased concentrations of free AA in cells and medium, indicating that such AA was released from [3 H]-labeled lipids (p<0.01). This lipid was mostly phosphatidylinositol, acted upon after activation of a protein kinase A (PKA)/cytoplasmic phospholipase A2 (cPLA2). In NP-exposed SC, an increase of diacylglycerols (DAG) also took place in both, cells and medium (p<0.01). Part of such DAG may have served as second messengers, since NP-increased DAG were associated with an augmented production of PGE2 and expression (mRNA) of COX2. Since the network of vimentin intermediate filaments is important for intracellular lipid transport, the effects of NP on the structure of this network in relation to the formation of cytoplasmic lipid droplets (LD) was studied. In NP-treated SC, the vimentin network was redistributed and the LD size was increased. The NP-dependent cytoskeletal redistribution was prevented by preincubation with H89, a PKA inhibitor. The formation of large LD was prevented by preincubation with either H89 or MEP, a PLA2 inhibitor, suggesting the participation of PKA and cPLA2 in LD biogenesis. We conclude that NP is involved in activating the proinflammatory pathway in SC, by providing the AA that is necessary for prostaglandin synthesis via PKA/PLA2 on the one hand, and by generating the DAG that is required as cofactor of the PKC-mediated activation of the NF- κβ/Cox-2 inflammatory pathway on the other.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/237017
Nonylphenol Induces Cytoskeletal Changes And Release Of Proinflammatory Mediators In Rat Sertoli Cells In Vitro; LXII Reunión Científica de la Sociedad Argentina de Investigación Clínica; LIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica ; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología; Buenos Aires; Argentina; 2017; 123-123
0025-7680
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/237017
identifier_str_mv Nonylphenol Induces Cytoskeletal Changes And Release Of Proinflammatory Mediators In Rat Sertoli Cells In Vitro; LXII Reunión Científica de la Sociedad Argentina de Investigación Clínica; LIII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica ; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología; Buenos Aires; Argentina; 2017; 123-123
0025-7680
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1854322148705828864
score 13.065482

Publicaciones similares