Cell-type-specific regulation of genes involved in testicular lipid metabolism: fatty acid-binding proteins, diacylglycerol acyltransferases and perilipin 2

Autores
Oresti, Gerardo Martin; García Lopéz, Jesus; Aveldaño, Marta Isabel; del Mazo, Jesús
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Male germ cell differentiation entails the synthesis and remodeling of membrane polar lipids and the formation of triacylglycerols (TAGs). This requires fatty acid-binding proteins (FABPs) for intracellular fatty acid traffic, a diacylglycerol acyltransferase (DGAT) to catalyze the final step of TAG biosynthesis, and a TAG storage mode. We examined the expression of genes encoding five members of the FABP family and two DGAT proteins, as well as the lipid droplet protein perilipin 2 (PLIN2), during mouse testis development and in specific cells from seminiferous epithelium. Fabp5 expression was distinctive of Sertoli cells and consequently was higher in prepubertal than in adult testis. The expression of Fabp3 increased in testis during postnatal development, associated with the functional differentiation of interstitial cells, but was low in germ cells. Fabp9, together with Fabp12, was prominently expressed in the latter. Their transcripts increased from spermatocytes to spermatids and, interestingly, were highest in spermatid-derived residual bodies (RB). Both Sertoli and germ cells, which produce neutral lipids and store them in lipid droplets, expressed Plin2. Yet, while Dgat1 was detected in Sertoli cells, Dgat2 accumulated in germ cells with a similar pattern of expression as Fabp9. These results correlated with polyunsaturated fatty acid-rich TAG levels also increasing with mouse germ cell differentiation highest in RB, connecting DGAT2 with the biosynthesis of such TAGs. The age- and germ cell type-associated increases in Fabp9, Dgat2, and Plin2 levels are thus functionally related in the last stages of germ cell differentiation.
Fil: Oresti, Gerardo Martin. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Cientí­ficas y Técnicas. Centro Científico Tecnológico Bahí­a Blanca. Instituto de Investigaciones Bioquímicas Bahí­a Blanca (i); Argentina
Fil: García Lopéz, Jesus. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas; España
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Cientí­ficas y Técnicas. Centro Científico Tecnológico Bahí­a Blanca. Instituto de Investigaciones Bioquímicas Bahí­a Blanca (i); Argentina
Fil: del Mazo, Jesús. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas; España. Consejo Nacional de Investigaciones Cientí­ficas y Técnicas. Centro Científico Tecnológico Bahí­a Blanca. Instituto de Investigaciones Bioquímicas Bahí­a Blanca (i); Argentina
Materia
Fabp
Testis
Germ Cells
Dgat
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4665
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4665
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cell-type-specific regulation of genes involved in testicular lipid metabolism: fatty acid-binding proteins, diacylglycerol acyltransferases and perilipin 2Oresti, Gerardo MartinGarcía Lopéz, JesusAveldaño, Marta Isabeldel Mazo, JesúsFabpTestisGerm CellsDgathttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Male germ cell differentiation entails the synthesis and remodeling of membrane polar lipids and the formation of triacylglycerols (TAGs). This requires fatty acid-binding proteins (FABPs) for intracellular fatty acid traffic, a diacylglycerol acyltransferase (DGAT) to catalyze the final step of TAG biosynthesis, and a TAG storage mode. We examined the expression of genes encoding five members of the FABP family and two DGAT proteins, as well as the lipid droplet protein perilipin 2 (PLIN2), during mouse testis development and in specific cells from seminiferous epithelium. Fabp5 expression was distinctive of Sertoli cells and consequently was higher in prepubertal than in adult testis. The expression of Fabp3 increased in testis during postnatal development, associated with the functional differentiation of interstitial cells, but was low in germ cells. Fabp9, together with Fabp12, was prominently expressed in the latter. Their transcripts increased from spermatocytes to spermatids and, interestingly, were highest in spermatid-derived residual bodies (RB). Both Sertoli and germ cells, which produce neutral lipids and store them in lipid droplets, expressed Plin2. Yet, while Dgat1 was detected in Sertoli cells, Dgat2 accumulated in germ cells with a similar pattern of expression as Fabp9. These results correlated with polyunsaturated fatty acid-rich TAG levels also increasing with mouse germ cell differentiation highest in RB, connecting DGAT2 with the biosynthesis of such TAGs. The age- and germ cell type-associated increases in Fabp9, Dgat2, and Plin2 levels are thus functionally related in the last stages of germ cell differentiation.Fil: Oresti, Gerardo Martin. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Cientí­ficas y Técnicas. Centro Científico Tecnológico Bahí­a Blanca. Instituto de Investigaciones Bioquímicas Bahí­a Blanca (i); ArgentinaFil: García Lopéz, Jesus. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas; EspañaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Cientí­ficas y Técnicas. Centro Científico Tecnológico Bahí­a Blanca. Instituto de Investigaciones Bioquímicas Bahí­a Blanca (i); ArgentinaFil: del Mazo, Jesús. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas; España. Consejo Nacional de Investigaciones Cientí­ficas y Técnicas. Centro Científico Tecnológico Bahí­a Blanca. Instituto de Investigaciones Bioquímicas Bahí­a Blanca (i); ArgentinaBioscientifica2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4665Oresti, Gerardo Martin; García Lopéz, Jesus; Aveldaño, Marta Isabel; del Mazo, Jesús; Cell-type-specific regulation of genes involved in testicular lipid metabolism: fatty acid-binding proteins, diacylglycerol acyltransferases and perilipin 2; Bioscientifica; Reproduction; 146; 10-2013; 471-4801470-1626enginfo:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/146/5/471.longinfo:eu-repo/semantics/altIdentifier/doi/10.1530/REP-13-0199info:eu-repo/semantics/altIdentifier/issn/1470-1626info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:50Zoai:ri.conicet.gov.ar:11336/4665instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:50.378CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cell-type-specific regulation of genes involved in testicular lipid metabolism: fatty acid-binding proteins, diacylglycerol acyltransferases and perilipin 2
title Cell-type-specific regulation of genes involved in testicular lipid metabolism: fatty acid-binding proteins, diacylglycerol acyltransferases and perilipin 2
spellingShingle Cell-type-specific regulation of genes involved in testicular lipid metabolism: fatty acid-binding proteins, diacylglycerol acyltransferases and perilipin 2
Oresti, Gerardo Martin
Fabp
Testis
Germ Cells
Dgat
title_short Cell-type-specific regulation of genes involved in testicular lipid metabolism: fatty acid-binding proteins, diacylglycerol acyltransferases and perilipin 2
title_full Cell-type-specific regulation of genes involved in testicular lipid metabolism: fatty acid-binding proteins, diacylglycerol acyltransferases and perilipin 2
title_fullStr Cell-type-specific regulation of genes involved in testicular lipid metabolism: fatty acid-binding proteins, diacylglycerol acyltransferases and perilipin 2
title_full_unstemmed Cell-type-specific regulation of genes involved in testicular lipid metabolism: fatty acid-binding proteins, diacylglycerol acyltransferases and perilipin 2
title_sort Cell-type-specific regulation of genes involved in testicular lipid metabolism: fatty acid-binding proteins, diacylglycerol acyltransferases and perilipin 2
dc.creator.none.fl_str_mv Oresti, Gerardo Martin
García Lopéz, Jesus
Aveldaño, Marta Isabel
del Mazo, Jesús
author Oresti, Gerardo Martin
author_facet Oresti, Gerardo Martin
García Lopéz, Jesus
Aveldaño, Marta Isabel
del Mazo, Jesús
author_role author
author2 García Lopéz, Jesus
Aveldaño, Marta Isabel
del Mazo, Jesús
author2_role author
author
author
dc.subject.none.fl_str_mv Fabp
Testis
Germ Cells
Dgat
topic Fabp
Testis
Germ Cells
Dgat
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Male germ cell differentiation entails the synthesis and remodeling of membrane polar lipids and the formation of triacylglycerols (TAGs). This requires fatty acid-binding proteins (FABPs) for intracellular fatty acid traffic, a diacylglycerol acyltransferase (DGAT) to catalyze the final step of TAG biosynthesis, and a TAG storage mode. We examined the expression of genes encoding five members of the FABP family and two DGAT proteins, as well as the lipid droplet protein perilipin 2 (PLIN2), during mouse testis development and in specific cells from seminiferous epithelium. Fabp5 expression was distinctive of Sertoli cells and consequently was higher in prepubertal than in adult testis. The expression of Fabp3 increased in testis during postnatal development, associated with the functional differentiation of interstitial cells, but was low in germ cells. Fabp9, together with Fabp12, was prominently expressed in the latter. Their transcripts increased from spermatocytes to spermatids and, interestingly, were highest in spermatid-derived residual bodies (RB). Both Sertoli and germ cells, which produce neutral lipids and store them in lipid droplets, expressed Plin2. Yet, while Dgat1 was detected in Sertoli cells, Dgat2 accumulated in germ cells with a similar pattern of expression as Fabp9. These results correlated with polyunsaturated fatty acid-rich TAG levels also increasing with mouse germ cell differentiation highest in RB, connecting DGAT2 with the biosynthesis of such TAGs. The age- and germ cell type-associated increases in Fabp9, Dgat2, and Plin2 levels are thus functionally related in the last stages of germ cell differentiation.
Fil: Oresti, Gerardo Martin. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Cientí­ficas y Técnicas. Centro Científico Tecnológico Bahí­a Blanca. Instituto de Investigaciones Bioquímicas Bahí­a Blanca (i); Argentina
Fil: García Lopéz, Jesus. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas; España
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Cientí­ficas y Técnicas. Centro Científico Tecnológico Bahí­a Blanca. Instituto de Investigaciones Bioquímicas Bahí­a Blanca (i); Argentina
Fil: del Mazo, Jesús. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas; España. Consejo Nacional de Investigaciones Cientí­ficas y Técnicas. Centro Científico Tecnológico Bahí­a Blanca. Instituto de Investigaciones Bioquímicas Bahí­a Blanca (i); Argentina
description Male germ cell differentiation entails the synthesis and remodeling of membrane polar lipids and the formation of triacylglycerols (TAGs). This requires fatty acid-binding proteins (FABPs) for intracellular fatty acid traffic, a diacylglycerol acyltransferase (DGAT) to catalyze the final step of TAG biosynthesis, and a TAG storage mode. We examined the expression of genes encoding five members of the FABP family and two DGAT proteins, as well as the lipid droplet protein perilipin 2 (PLIN2), during mouse testis development and in specific cells from seminiferous epithelium. Fabp5 expression was distinctive of Sertoli cells and consequently was higher in prepubertal than in adult testis. The expression of Fabp3 increased in testis during postnatal development, associated with the functional differentiation of interstitial cells, but was low in germ cells. Fabp9, together with Fabp12, was prominently expressed in the latter. Their transcripts increased from spermatocytes to spermatids and, interestingly, were highest in spermatid-derived residual bodies (RB). Both Sertoli and germ cells, which produce neutral lipids and store them in lipid droplets, expressed Plin2. Yet, while Dgat1 was detected in Sertoli cells, Dgat2 accumulated in germ cells with a similar pattern of expression as Fabp9. These results correlated with polyunsaturated fatty acid-rich TAG levels also increasing with mouse germ cell differentiation highest in RB, connecting DGAT2 with the biosynthesis of such TAGs. The age- and germ cell type-associated increases in Fabp9, Dgat2, and Plin2 levels are thus functionally related in the last stages of germ cell differentiation.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4665
Oresti, Gerardo Martin; García Lopéz, Jesus; Aveldaño, Marta Isabel; del Mazo, Jesús; Cell-type-specific regulation of genes involved in testicular lipid metabolism: fatty acid-binding proteins, diacylglycerol acyltransferases and perilipin 2; Bioscientifica; Reproduction; 146; 10-2013; 471-480
1470-1626
url http://hdl.handle.net/11336/4665
identifier_str_mv Oresti, Gerardo Martin; García Lopéz, Jesus; Aveldaño, Marta Isabel; del Mazo, Jesús; Cell-type-specific regulation of genes involved in testicular lipid metabolism: fatty acid-binding proteins, diacylglycerol acyltransferases and perilipin 2; Bioscientifica; Reproduction; 146; 10-2013; 471-480
1470-1626
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/146/5/471.long
info:eu-repo/semantics/altIdentifier/doi/10.1530/REP-13-0199
info:eu-repo/semantics/altIdentifier/issn/1470-1626
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bioscientifica
publisher.none.fl_str_mv Bioscientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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