Prenatal exposure to pesticides disrupts testicular histoarchitecture and alters testosterone levels in male Caiman latirostris

Autores
Rey, Florencia; Gonzalez, Marianela; Zayas, Marcelo Alejandro; Stoker, Cora; Durando, Milena de Lourdes; Luque, Enrique Hugo; Muñoz de Toro, Monica Milagros
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The increased use of agrochemical pesticides, such as atrazine (ATZ) and endosulfan (END), may have a significant impact on ecosystem health and biodiversity. The aim of this study was to investigate the consequences of in ovum exposure to ATZ and END on Caiman latirostris gonadal histo-functional features. Caiman eggs were collected from environmentally pristine areas and incubated in controlled conditions at male producing temperature (33C). At stage 20 of embryonic development, the sensitive stage for gonadal sex determination, eggs were exposed to one dose of either END or ATZ. Gonadal histo-morphology was examined in caiman hatchlings and serum levels of testosterone were measured. Regardless of treatment condition, all eggs incubated at 33 C resulted in male hatchlings. Tortuous seminiferous tubules with increased perimeter, disrupted distribution of peritubular myoid cells (desmin positive), and emptied tubular lumens characterized the testes of pesticide-exposed caiman. An imbalance between proliferative activity and cell death was observed in the testes of caiman exposed to the higher doses of END, mainly due to a high frequency of apoptosis in intratubular cells. This altered cell turnover was associated with decreased testosterone levels. Prenatal exposure to only one dose of END and ATZ disrupted neonatal male gonadal histo-functional features. Alterations described here could have detrimental effects on the sexual maturation of the caiman and, ultimately, on the success of male caiman reproduction.
Fil: Rey, Florencia. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez, Marianela. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zayas, Marcelo Alejandro. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Stoker, Cora. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Durando, Milena de Lourdes. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Luque, Enrique Hugo. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Muñoz de Toro, Monica Milagros. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Caiman Latirostris
Endosulfan
Atrazine
Endocrine Disruptors
Testes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25439
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Prenatal exposure to pesticides disrupts testicular histoarchitecture and alters testosterone levels in male Caiman latirostrisRey, FlorenciaGonzalez, MarianelaZayas, Marcelo AlejandroStoker, CoraDurando, Milena de LourdesLuque, Enrique HugoMuñoz de Toro, Monica MilagrosCaiman LatirostrisEndosulfanAtrazineEndocrine DisruptorsTestesThe increased use of agrochemical pesticides, such as atrazine (ATZ) and endosulfan (END), may have a significant impact on ecosystem health and biodiversity. The aim of this study was to investigate the consequences of in ovum exposure to ATZ and END on Caiman latirostris gonadal histo-functional features. Caiman eggs were collected from environmentally pristine areas and incubated in controlled conditions at male producing temperature (33C). At stage 20 of embryonic development, the sensitive stage for gonadal sex determination, eggs were exposed to one dose of either END or ATZ. Gonadal histo-morphology was examined in caiman hatchlings and serum levels of testosterone were measured. Regardless of treatment condition, all eggs incubated at 33 C resulted in male hatchlings. Tortuous seminiferous tubules with increased perimeter, disrupted distribution of peritubular myoid cells (desmin positive), and emptied tubular lumens characterized the testes of pesticide-exposed caiman. An imbalance between proliferative activity and cell death was observed in the testes of caiman exposed to the higher doses of END, mainly due to a high frequency of apoptosis in intratubular cells. This altered cell turnover was associated with decreased testosterone levels. Prenatal exposure to only one dose of END and ATZ disrupted neonatal male gonadal histo-functional features. Alterations described here could have detrimental effects on the sexual maturation of the caiman and, ultimately, on the success of male caiman reproduction.Fil: Rey, Florencia. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gonzalez, Marianela. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zayas, Marcelo Alejandro. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Stoker, Cora. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Durando, Milena de Lourdes. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Luque, Enrique Hugo. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Muñoz de Toro, Monica Milagros. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2009-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25439Rey, Florencia; Gonzalez, Marianela; Zayas, Marcelo Alejandro; Stoker, Cora; Durando, Milena de Lourdes; et al.; Prenatal exposure to pesticides disrupts testicular histoarchitecture and alters testosterone levels in male Caiman latirostris; Elsevier; General and Comparative Endocrinology; 162; 3; 7-2009; 286-2920016-6480CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0016648009001464info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ygcen.2009.03.032info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:49Zoai:ri.conicet.gov.ar:11336/25439instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:50.007CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Prenatal exposure to pesticides disrupts testicular histoarchitecture and alters testosterone levels in male Caiman latirostris
title Prenatal exposure to pesticides disrupts testicular histoarchitecture and alters testosterone levels in male Caiman latirostris
spellingShingle Prenatal exposure to pesticides disrupts testicular histoarchitecture and alters testosterone levels in male Caiman latirostris
Rey, Florencia
Caiman Latirostris
Endosulfan
Atrazine
Endocrine Disruptors
Testes
title_short Prenatal exposure to pesticides disrupts testicular histoarchitecture and alters testosterone levels in male Caiman latirostris
title_full Prenatal exposure to pesticides disrupts testicular histoarchitecture and alters testosterone levels in male Caiman latirostris
title_fullStr Prenatal exposure to pesticides disrupts testicular histoarchitecture and alters testosterone levels in male Caiman latirostris
title_full_unstemmed Prenatal exposure to pesticides disrupts testicular histoarchitecture and alters testosterone levels in male Caiman latirostris
title_sort Prenatal exposure to pesticides disrupts testicular histoarchitecture and alters testosterone levels in male Caiman latirostris
dc.creator.none.fl_str_mv Rey, Florencia
Gonzalez, Marianela
Zayas, Marcelo Alejandro
Stoker, Cora
Durando, Milena de Lourdes
Luque, Enrique Hugo
Muñoz de Toro, Monica Milagros
author Rey, Florencia
author_facet Rey, Florencia
Gonzalez, Marianela
Zayas, Marcelo Alejandro
Stoker, Cora
Durando, Milena de Lourdes
Luque, Enrique Hugo
Muñoz de Toro, Monica Milagros
author_role author
author2 Gonzalez, Marianela
Zayas, Marcelo Alejandro
Stoker, Cora
Durando, Milena de Lourdes
Luque, Enrique Hugo
Muñoz de Toro, Monica Milagros
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Caiman Latirostris
Endosulfan
Atrazine
Endocrine Disruptors
Testes
topic Caiman Latirostris
Endosulfan
Atrazine
Endocrine Disruptors
Testes
dc.description.none.fl_txt_mv The increased use of agrochemical pesticides, such as atrazine (ATZ) and endosulfan (END), may have a significant impact on ecosystem health and biodiversity. The aim of this study was to investigate the consequences of in ovum exposure to ATZ and END on Caiman latirostris gonadal histo-functional features. Caiman eggs were collected from environmentally pristine areas and incubated in controlled conditions at male producing temperature (33C). At stage 20 of embryonic development, the sensitive stage for gonadal sex determination, eggs were exposed to one dose of either END or ATZ. Gonadal histo-morphology was examined in caiman hatchlings and serum levels of testosterone were measured. Regardless of treatment condition, all eggs incubated at 33 C resulted in male hatchlings. Tortuous seminiferous tubules with increased perimeter, disrupted distribution of peritubular myoid cells (desmin positive), and emptied tubular lumens characterized the testes of pesticide-exposed caiman. An imbalance between proliferative activity and cell death was observed in the testes of caiman exposed to the higher doses of END, mainly due to a high frequency of apoptosis in intratubular cells. This altered cell turnover was associated with decreased testosterone levels. Prenatal exposure to only one dose of END and ATZ disrupted neonatal male gonadal histo-functional features. Alterations described here could have detrimental effects on the sexual maturation of the caiman and, ultimately, on the success of male caiman reproduction.
Fil: Rey, Florencia. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez, Marianela. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zayas, Marcelo Alejandro. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Stoker, Cora. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Durando, Milena de Lourdes. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Luque, Enrique Hugo. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Muñoz de Toro, Monica Milagros. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The increased use of agrochemical pesticides, such as atrazine (ATZ) and endosulfan (END), may have a significant impact on ecosystem health and biodiversity. The aim of this study was to investigate the consequences of in ovum exposure to ATZ and END on Caiman latirostris gonadal histo-functional features. Caiman eggs were collected from environmentally pristine areas and incubated in controlled conditions at male producing temperature (33C). At stage 20 of embryonic development, the sensitive stage for gonadal sex determination, eggs were exposed to one dose of either END or ATZ. Gonadal histo-morphology was examined in caiman hatchlings and serum levels of testosterone were measured. Regardless of treatment condition, all eggs incubated at 33 C resulted in male hatchlings. Tortuous seminiferous tubules with increased perimeter, disrupted distribution of peritubular myoid cells (desmin positive), and emptied tubular lumens characterized the testes of pesticide-exposed caiman. An imbalance between proliferative activity and cell death was observed in the testes of caiman exposed to the higher doses of END, mainly due to a high frequency of apoptosis in intratubular cells. This altered cell turnover was associated with decreased testosterone levels. Prenatal exposure to only one dose of END and ATZ disrupted neonatal male gonadal histo-functional features. Alterations described here could have detrimental effects on the sexual maturation of the caiman and, ultimately, on the success of male caiman reproduction.
publishDate 2009
dc.date.none.fl_str_mv 2009-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25439
Rey, Florencia; Gonzalez, Marianela; Zayas, Marcelo Alejandro; Stoker, Cora; Durando, Milena de Lourdes; et al.; Prenatal exposure to pesticides disrupts testicular histoarchitecture and alters testosterone levels in male Caiman latirostris; Elsevier; General and Comparative Endocrinology; 162; 3; 7-2009; 286-292
0016-6480
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25439
identifier_str_mv Rey, Florencia; Gonzalez, Marianela; Zayas, Marcelo Alejandro; Stoker, Cora; Durando, Milena de Lourdes; et al.; Prenatal exposure to pesticides disrupts testicular histoarchitecture and alters testosterone levels in male Caiman latirostris; Elsevier; General and Comparative Endocrinology; 162; 3; 7-2009; 286-292
0016-6480
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ygcen.2009.03.032
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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