Metabarcoding analysis of the fungal biodiversity associated with Castaño Overa Glacier – Mount Tronador, Patagonia, Argentina

Autores
Duo Saito, Rubí Azul; Connell, L.; Rodriguez, R.; Redman, Damian Patricio; Libkind Frati, Diego; de Garcia, Virginia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cold environments represent the largest fraction of Earth's biosphere, and are known habitats for cold-adapted microorganisms. The aim of this study was to assess the occurrence and biodiversity of cold-adapted fungi associated with the Castaño Overo glacier, Mount Tronador, Patagonia, Argentina. Samples of naked soil, glacial ice and snow were collected and analyzed using tag-encoded 454 pyrosequencing of the nuclear ribosomal internal transcribed spacer (ITS). A total of 1082 OTUs (operational taxonomic units) and 151,669 sequences were obtained. OTUs obtained from soil samples corresponded mainly to the phylum Ascomycota, whereas for snow and ice samples the phylum Basidiomycota was the most represented group. Metabarcoding analysis showed high biodiversity in glacial environments and allowed the detection of hitherto unknown taxa for Patagonia. To the best of our knowledge, this is the first report of fungal diversity in an extreme glacial environment of Patagonian Argentina using amplicon sequencing.
Fil: Duo Saito, Rubí Azul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales.; Argentina
Fil: Connell, L.. University Of Maine; Estados Unidos
Fil: Rodriguez, R.. Adaptive Symbiotic Technologies; Estados Unidos
Fil: Redman, Damian Patricio. Adaptive Symbiotic Technologies; Estados Unidos
Fil: Libkind Frati, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales.; Argentina
Fil: de Garcia, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina
Materia
EXTREMOPHILIC
MICROBOTRYOMYCETES
NON-POLAR GLACIER
OLIGOTROPHIC ENVIRONMENT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/88806
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Metabarcoding analysis of the fungal biodiversity associated with Castaño Overa Glacier – Mount Tronador, Patagonia, ArgentinaDuo Saito, Rubí AzulConnell, L.Rodriguez, R.Redman, Damian PatricioLibkind Frati, Diegode Garcia, VirginiaEXTREMOPHILICMICROBOTRYOMYCETESNON-POLAR GLACIEROLIGOTROPHIC ENVIRONMENThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cold environments represent the largest fraction of Earth's biosphere, and are known habitats for cold-adapted microorganisms. The aim of this study was to assess the occurrence and biodiversity of cold-adapted fungi associated with the Castaño Overo glacier, Mount Tronador, Patagonia, Argentina. Samples of naked soil, glacial ice and snow were collected and analyzed using tag-encoded 454 pyrosequencing of the nuclear ribosomal internal transcribed spacer (ITS). A total of 1082 OTUs (operational taxonomic units) and 151,669 sequences were obtained. OTUs obtained from soil samples corresponded mainly to the phylum Ascomycota, whereas for snow and ice samples the phylum Basidiomycota was the most represented group. Metabarcoding analysis showed high biodiversity in glacial environments and allowed the detection of hitherto unknown taxa for Patagonia. To the best of our knowledge, this is the first report of fungal diversity in an extreme glacial environment of Patagonian Argentina using amplicon sequencing.Fil: Duo Saito, Rubí Azul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales.; ArgentinaFil: Connell, L.. University Of Maine; Estados UnidosFil: Rodriguez, R.. Adaptive Symbiotic Technologies; Estados UnidosFil: Redman, Damian Patricio. Adaptive Symbiotic Technologies; Estados UnidosFil: Libkind Frati, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales.; ArgentinaFil: de Garcia, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; ArgentinaElsevier2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/88806Duo Saito, Rubí Azul; Connell, L.; Rodriguez, R.; Redman, Damian Patricio; Libkind Frati, Diego; et al.; Metabarcoding analysis of the fungal biodiversity associated with Castaño Overa Glacier – Mount Tronador, Patagonia, Argentina; Elsevier; Fungal Ecology; 36; 12-2018; 8-161754-5048CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S175450481830076Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.funeco.2018.07.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:38Zoai:ri.conicet.gov.ar:11336/88806instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:39.01CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Metabarcoding analysis of the fungal biodiversity associated with Castaño Overa Glacier – Mount Tronador, Patagonia, Argentina
title Metabarcoding analysis of the fungal biodiversity associated with Castaño Overa Glacier – Mount Tronador, Patagonia, Argentina
spellingShingle Metabarcoding analysis of the fungal biodiversity associated with Castaño Overa Glacier – Mount Tronador, Patagonia, Argentina
Duo Saito, Rubí Azul
EXTREMOPHILIC
MICROBOTRYOMYCETES
NON-POLAR GLACIER
OLIGOTROPHIC ENVIRONMENT
title_short Metabarcoding analysis of the fungal biodiversity associated with Castaño Overa Glacier – Mount Tronador, Patagonia, Argentina
title_full Metabarcoding analysis of the fungal biodiversity associated with Castaño Overa Glacier – Mount Tronador, Patagonia, Argentina
title_fullStr Metabarcoding analysis of the fungal biodiversity associated with Castaño Overa Glacier – Mount Tronador, Patagonia, Argentina
title_full_unstemmed Metabarcoding analysis of the fungal biodiversity associated with Castaño Overa Glacier – Mount Tronador, Patagonia, Argentina
title_sort Metabarcoding analysis of the fungal biodiversity associated with Castaño Overa Glacier – Mount Tronador, Patagonia, Argentina
dc.creator.none.fl_str_mv Duo Saito, Rubí Azul
Connell, L.
Rodriguez, R.
Redman, Damian Patricio
Libkind Frati, Diego
de Garcia, Virginia
author Duo Saito, Rubí Azul
author_facet Duo Saito, Rubí Azul
Connell, L.
Rodriguez, R.
Redman, Damian Patricio
Libkind Frati, Diego
de Garcia, Virginia
author_role author
author2 Connell, L.
Rodriguez, R.
Redman, Damian Patricio
Libkind Frati, Diego
de Garcia, Virginia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv EXTREMOPHILIC
MICROBOTRYOMYCETES
NON-POLAR GLACIER
OLIGOTROPHIC ENVIRONMENT
topic EXTREMOPHILIC
MICROBOTRYOMYCETES
NON-POLAR GLACIER
OLIGOTROPHIC ENVIRONMENT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cold environments represent the largest fraction of Earth's biosphere, and are known habitats for cold-adapted microorganisms. The aim of this study was to assess the occurrence and biodiversity of cold-adapted fungi associated with the Castaño Overo glacier, Mount Tronador, Patagonia, Argentina. Samples of naked soil, glacial ice and snow were collected and analyzed using tag-encoded 454 pyrosequencing of the nuclear ribosomal internal transcribed spacer (ITS). A total of 1082 OTUs (operational taxonomic units) and 151,669 sequences were obtained. OTUs obtained from soil samples corresponded mainly to the phylum Ascomycota, whereas for snow and ice samples the phylum Basidiomycota was the most represented group. Metabarcoding analysis showed high biodiversity in glacial environments and allowed the detection of hitherto unknown taxa for Patagonia. To the best of our knowledge, this is the first report of fungal diversity in an extreme glacial environment of Patagonian Argentina using amplicon sequencing.
Fil: Duo Saito, Rubí Azul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales.; Argentina
Fil: Connell, L.. University Of Maine; Estados Unidos
Fil: Rodriguez, R.. Adaptive Symbiotic Technologies; Estados Unidos
Fil: Redman, Damian Patricio. Adaptive Symbiotic Technologies; Estados Unidos
Fil: Libkind Frati, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales.; Argentina
Fil: de Garcia, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina
description Cold environments represent the largest fraction of Earth's biosphere, and are known habitats for cold-adapted microorganisms. The aim of this study was to assess the occurrence and biodiversity of cold-adapted fungi associated with the Castaño Overo glacier, Mount Tronador, Patagonia, Argentina. Samples of naked soil, glacial ice and snow were collected and analyzed using tag-encoded 454 pyrosequencing of the nuclear ribosomal internal transcribed spacer (ITS). A total of 1082 OTUs (operational taxonomic units) and 151,669 sequences were obtained. OTUs obtained from soil samples corresponded mainly to the phylum Ascomycota, whereas for snow and ice samples the phylum Basidiomycota was the most represented group. Metabarcoding analysis showed high biodiversity in glacial environments and allowed the detection of hitherto unknown taxa for Patagonia. To the best of our knowledge, this is the first report of fungal diversity in an extreme glacial environment of Patagonian Argentina using amplicon sequencing.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/88806
Duo Saito, Rubí Azul; Connell, L.; Rodriguez, R.; Redman, Damian Patricio; Libkind Frati, Diego; et al.; Metabarcoding analysis of the fungal biodiversity associated with Castaño Overa Glacier – Mount Tronador, Patagonia, Argentina; Elsevier; Fungal Ecology; 36; 12-2018; 8-16
1754-5048
CONICET Digital
CONICET
url http://hdl.handle.net/11336/88806
identifier_str_mv Duo Saito, Rubí Azul; Connell, L.; Rodriguez, R.; Redman, Damian Patricio; Libkind Frati, Diego; et al.; Metabarcoding analysis of the fungal biodiversity associated with Castaño Overa Glacier – Mount Tronador, Patagonia, Argentina; Elsevier; Fungal Ecology; 36; 12-2018; 8-16
1754-5048
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S175450481830076X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.funeco.2018.07.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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