Biogeochemical controls on the bacterial population in the eastern Atlantic Ocean

Autores
Neogi, Sucharit Basu; Koch, Boris Peter; Schmitt Kopplin, Philippe; Pohl, Christine; Kattner, Gerhard; Yamasaki, Shintaro; Lara, Ruben Jose
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Little is known about bacterial dynamics in the oligotrophic ocean, particularly about cultivable bacteria. We examined the abundance of total and cultivable bacteria in relation to changes in biogeochemical conditions in the eastern Atlantic Ocean with special regard to Vibrio spp., a group of bacteria that can cause diseases in human and aquatic organisms. Surface, deep water and plankton (<20 µm, 20– 55 µm and >55 µm) samples were collected between 50◦ N and 24◦ S. Chlorophyll-a was very low (<0.3 µg l−1 ) in most areas of the nutrient-poor Atlantic, except at a few locations near upwelling regions. In surface water, dissolved organic carbon (DOC) and nitrogen (DON) concentrations were 64– 95 µM C and 2–10 µM N accounting for ≥90 % and ≥76 % of total organic C and N, respectively. DOC and DON gradually decreased to ∼45 µM C and <5 µM N in the bottom water. In the surface layer, culture independent total bacteria and other prokaryotes represented by 40 -6-diamidino- 2-phenylindole (DAPI) counts, ranged mostly between 107 and 108 cells l−1 , while cultivable bacterial counts (CBC) and Vibrio spp. were found at concentrations of 104–107 and 102–105 colony forming units (CFU) l−1 , respectively. Most bacteria (>99 %) were found in the nanoplankton fraction (<20 µm), however, bacterial abundance did not correlate with suspended particulates (chlorophyll-a, particulate organic C [POC] and N [PON]). Instead, we found a highly significant correlation between bacterial abundance and temperature (p < 0.001) and a significant correlation with DOC and DON (p < 0.005 and < 0.01, respectively). In comparison to CBC and DAPI-stained prokaryotes, cultivable Vibrio showed a stronger and highly significant correlation with DOC and DON (p < 0.0005 and p < 0.005, respectively). In cold waters of the mesopelagic and abyssal zones, CBC was 50 to 100-times lower than in the surface layer; however, cultivable Vibrio spp. could be isolated from the bathypelagic zone and even near the seafloor (average ∼10 CFU l−1 ). The depth-wise decrease in CBC and Vibrio coincided with the decrease in both DOC and POC. Our study indicates that Vibrio and other bacteria may largely depend on dissolved organic matter to survive in nutrient-poor oceanic habitats.
Fil: Neogi, Sucharit Basu. Osaka Prefecture University; Japón. International Centre for Diarrhoeal Disease Research; Bangladesh
Fil: Koch, Boris Peter. Alfred Wegener Institute for Polar and Marine Research; Alemania. University of Applied Sciences; Alemania
Fil: Schmitt Kopplin, Philippe. Helmholtz Zentrum Munchen; Alemania
Fil: Pohl, Christine. Leibniz Institute for Baltic Sea Research; Alemania
Fil: Kattner, Gerhard. Alfred Wegener Institute for Polar and Marine Research; Alemania
Fil: Yamasaki, Shintaro. Osaka Prefecture University; Japón
Fil: Lara, Ruben Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina. Leibniz Centre for Tropical Marine Ecology GmbH; Alemania
Materia
Atlantic
oligotrophic
DOC-Iron
bacteria
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22291

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oai_identifier_str oai:ri.conicet.gov.ar:11336/22291
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biogeochemical controls on the bacterial population in the eastern Atlantic OceanNeogi, Sucharit BasuKoch, Boris PeterSchmitt Kopplin, PhilippePohl, ChristineKattner, GerhardYamasaki, ShintaroLara, Ruben JoseAtlanticoligotrophicDOC-Ironbacteriahttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Little is known about bacterial dynamics in the oligotrophic ocean, particularly about cultivable bacteria. We examined the abundance of total and cultivable bacteria in relation to changes in biogeochemical conditions in the eastern Atlantic Ocean with special regard to Vibrio spp., a group of bacteria that can cause diseases in human and aquatic organisms. Surface, deep water and plankton (<20 µm, 20– 55 µm and >55 µm) samples were collected between 50◦ N and 24◦ S. Chlorophyll-a was very low (<0.3 µg l−1 ) in most areas of the nutrient-poor Atlantic, except at a few locations near upwelling regions. In surface water, dissolved organic carbon (DOC) and nitrogen (DON) concentrations were 64– 95 µM C and 2–10 µM N accounting for ≥90 % and ≥76 % of total organic C and N, respectively. DOC and DON gradually decreased to ∼45 µM C and <5 µM N in the bottom water. In the surface layer, culture independent total bacteria and other prokaryotes represented by 40 -6-diamidino- 2-phenylindole (DAPI) counts, ranged mostly between 107 and 108 cells l−1 , while cultivable bacterial counts (CBC) and Vibrio spp. were found at concentrations of 104–107 and 102–105 colony forming units (CFU) l−1 , respectively. Most bacteria (>99 %) were found in the nanoplankton fraction (<20 µm), however, bacterial abundance did not correlate with suspended particulates (chlorophyll-a, particulate organic C [POC] and N [PON]). Instead, we found a highly significant correlation between bacterial abundance and temperature (p < 0.001) and a significant correlation with DOC and DON (p < 0.005 and < 0.01, respectively). In comparison to CBC and DAPI-stained prokaryotes, cultivable Vibrio showed a stronger and highly significant correlation with DOC and DON (p < 0.0005 and p < 0.005, respectively). In cold waters of the mesopelagic and abyssal zones, CBC was 50 to 100-times lower than in the surface layer; however, cultivable Vibrio spp. could be isolated from the bathypelagic zone and even near the seafloor (average ∼10 CFU l−1 ). The depth-wise decrease in CBC and Vibrio coincided with the decrease in both DOC and POC. Our study indicates that Vibrio and other bacteria may largely depend on dissolved organic matter to survive in nutrient-poor oceanic habitats.Fil: Neogi, Sucharit Basu. Osaka Prefecture University; Japón. International Centre for Diarrhoeal Disease Research; BangladeshFil: Koch, Boris Peter. Alfred Wegener Institute for Polar and Marine Research; Alemania. University of Applied Sciences; AlemaniaFil: Schmitt Kopplin, Philippe. Helmholtz Zentrum Munchen; AlemaniaFil: Pohl, Christine. Leibniz Institute for Baltic Sea Research; AlemaniaFil: Kattner, Gerhard. Alfred Wegener Institute for Polar and Marine Research; AlemaniaFil: Yamasaki, Shintaro. Osaka Prefecture University; JapónFil: Lara, Ruben Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina. Leibniz Centre for Tropical Marine Ecology GmbH; AlemaniaCopernicus Publications2011-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22291Neogi, Sucharit Basu; Koch, Boris Peter; Schmitt Kopplin, Philippe; Pohl, Christine; Kattner, Gerhard; et al.; Biogeochemical controls on the bacterial population in the eastern Atlantic Ocean; Copernicus Publications; Biogeosciences; 8; 7-2011; 3747-37591726-41701726-4189CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.5194/bg-8-3747-2011info:eu-repo/semantics/altIdentifier/url/https://www.biogeosciences.net/8/3747/2011/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:12Zoai:ri.conicet.gov.ar:11336/22291instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:13.022CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biogeochemical controls on the bacterial population in the eastern Atlantic Ocean
title Biogeochemical controls on the bacterial population in the eastern Atlantic Ocean
spellingShingle Biogeochemical controls on the bacterial population in the eastern Atlantic Ocean
Neogi, Sucharit Basu
Atlantic
oligotrophic
DOC-Iron
bacteria
title_short Biogeochemical controls on the bacterial population in the eastern Atlantic Ocean
title_full Biogeochemical controls on the bacterial population in the eastern Atlantic Ocean
title_fullStr Biogeochemical controls on the bacterial population in the eastern Atlantic Ocean
title_full_unstemmed Biogeochemical controls on the bacterial population in the eastern Atlantic Ocean
title_sort Biogeochemical controls on the bacterial population in the eastern Atlantic Ocean
dc.creator.none.fl_str_mv Neogi, Sucharit Basu
Koch, Boris Peter
Schmitt Kopplin, Philippe
Pohl, Christine
Kattner, Gerhard
Yamasaki, Shintaro
Lara, Ruben Jose
author Neogi, Sucharit Basu
author_facet Neogi, Sucharit Basu
Koch, Boris Peter
Schmitt Kopplin, Philippe
Pohl, Christine
Kattner, Gerhard
Yamasaki, Shintaro
Lara, Ruben Jose
author_role author
author2 Koch, Boris Peter
Schmitt Kopplin, Philippe
Pohl, Christine
Kattner, Gerhard
Yamasaki, Shintaro
Lara, Ruben Jose
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Atlantic
oligotrophic
DOC-Iron
bacteria
topic Atlantic
oligotrophic
DOC-Iron
bacteria
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Little is known about bacterial dynamics in the oligotrophic ocean, particularly about cultivable bacteria. We examined the abundance of total and cultivable bacteria in relation to changes in biogeochemical conditions in the eastern Atlantic Ocean with special regard to Vibrio spp., a group of bacteria that can cause diseases in human and aquatic organisms. Surface, deep water and plankton (<20 µm, 20– 55 µm and >55 µm) samples were collected between 50◦ N and 24◦ S. Chlorophyll-a was very low (<0.3 µg l−1 ) in most areas of the nutrient-poor Atlantic, except at a few locations near upwelling regions. In surface water, dissolved organic carbon (DOC) and nitrogen (DON) concentrations were 64– 95 µM C and 2–10 µM N accounting for ≥90 % and ≥76 % of total organic C and N, respectively. DOC and DON gradually decreased to ∼45 µM C and <5 µM N in the bottom water. In the surface layer, culture independent total bacteria and other prokaryotes represented by 40 -6-diamidino- 2-phenylindole (DAPI) counts, ranged mostly between 107 and 108 cells l−1 , while cultivable bacterial counts (CBC) and Vibrio spp. were found at concentrations of 104–107 and 102–105 colony forming units (CFU) l−1 , respectively. Most bacteria (>99 %) were found in the nanoplankton fraction (<20 µm), however, bacterial abundance did not correlate with suspended particulates (chlorophyll-a, particulate organic C [POC] and N [PON]). Instead, we found a highly significant correlation between bacterial abundance and temperature (p < 0.001) and a significant correlation with DOC and DON (p < 0.005 and < 0.01, respectively). In comparison to CBC and DAPI-stained prokaryotes, cultivable Vibrio showed a stronger and highly significant correlation with DOC and DON (p < 0.0005 and p < 0.005, respectively). In cold waters of the mesopelagic and abyssal zones, CBC was 50 to 100-times lower than in the surface layer; however, cultivable Vibrio spp. could be isolated from the bathypelagic zone and even near the seafloor (average ∼10 CFU l−1 ). The depth-wise decrease in CBC and Vibrio coincided with the decrease in both DOC and POC. Our study indicates that Vibrio and other bacteria may largely depend on dissolved organic matter to survive in nutrient-poor oceanic habitats.
Fil: Neogi, Sucharit Basu. Osaka Prefecture University; Japón. International Centre for Diarrhoeal Disease Research; Bangladesh
Fil: Koch, Boris Peter. Alfred Wegener Institute for Polar and Marine Research; Alemania. University of Applied Sciences; Alemania
Fil: Schmitt Kopplin, Philippe. Helmholtz Zentrum Munchen; Alemania
Fil: Pohl, Christine. Leibniz Institute for Baltic Sea Research; Alemania
Fil: Kattner, Gerhard. Alfred Wegener Institute for Polar and Marine Research; Alemania
Fil: Yamasaki, Shintaro. Osaka Prefecture University; Japón
Fil: Lara, Ruben Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina. Leibniz Centre for Tropical Marine Ecology GmbH; Alemania
description Little is known about bacterial dynamics in the oligotrophic ocean, particularly about cultivable bacteria. We examined the abundance of total and cultivable bacteria in relation to changes in biogeochemical conditions in the eastern Atlantic Ocean with special regard to Vibrio spp., a group of bacteria that can cause diseases in human and aquatic organisms. Surface, deep water and plankton (<20 µm, 20– 55 µm and >55 µm) samples were collected between 50◦ N and 24◦ S. Chlorophyll-a was very low (<0.3 µg l−1 ) in most areas of the nutrient-poor Atlantic, except at a few locations near upwelling regions. In surface water, dissolved organic carbon (DOC) and nitrogen (DON) concentrations were 64– 95 µM C and 2–10 µM N accounting for ≥90 % and ≥76 % of total organic C and N, respectively. DOC and DON gradually decreased to ∼45 µM C and <5 µM N in the bottom water. In the surface layer, culture independent total bacteria and other prokaryotes represented by 40 -6-diamidino- 2-phenylindole (DAPI) counts, ranged mostly between 107 and 108 cells l−1 , while cultivable bacterial counts (CBC) and Vibrio spp. were found at concentrations of 104–107 and 102–105 colony forming units (CFU) l−1 , respectively. Most bacteria (>99 %) were found in the nanoplankton fraction (<20 µm), however, bacterial abundance did not correlate with suspended particulates (chlorophyll-a, particulate organic C [POC] and N [PON]). Instead, we found a highly significant correlation between bacterial abundance and temperature (p < 0.001) and a significant correlation with DOC and DON (p < 0.005 and < 0.01, respectively). In comparison to CBC and DAPI-stained prokaryotes, cultivable Vibrio showed a stronger and highly significant correlation with DOC and DON (p < 0.0005 and p < 0.005, respectively). In cold waters of the mesopelagic and abyssal zones, CBC was 50 to 100-times lower than in the surface layer; however, cultivable Vibrio spp. could be isolated from the bathypelagic zone and even near the seafloor (average ∼10 CFU l−1 ). The depth-wise decrease in CBC and Vibrio coincided with the decrease in both DOC and POC. Our study indicates that Vibrio and other bacteria may largely depend on dissolved organic matter to survive in nutrient-poor oceanic habitats.
publishDate 2011
dc.date.none.fl_str_mv 2011-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22291
Neogi, Sucharit Basu; Koch, Boris Peter; Schmitt Kopplin, Philippe; Pohl, Christine; Kattner, Gerhard; et al.; Biogeochemical controls on the bacterial population in the eastern Atlantic Ocean; Copernicus Publications; Biogeosciences; 8; 7-2011; 3747-3759
1726-4170
1726-4189
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22291
identifier_str_mv Neogi, Sucharit Basu; Koch, Boris Peter; Schmitt Kopplin, Philippe; Pohl, Christine; Kattner, Gerhard; et al.; Biogeochemical controls on the bacterial population in the eastern Atlantic Ocean; Copernicus Publications; Biogeosciences; 8; 7-2011; 3747-3759
1726-4170
1726-4189
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.5194/bg-8-3747-2011
info:eu-repo/semantics/altIdentifier/url/https://www.biogeosciences.net/8/3747/2011/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Copernicus Publications
publisher.none.fl_str_mv Copernicus Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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