Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes
- Autores
- Ruano, Marta; Mateos Maroto, Ana; Ortega, Francisco; Ritacco, Hernán Alejandro; Fernández Rubio, José Emilio; Guzmán, Eduardo; Rubio, Ramón G.
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- This work presents a simple methodology for coating small unilamellar liposomes bearing different degrees of positive charge with polyelectrolyte multilayers using the sequential layer-by-layer deposition method. The liposomes were made of mixtures of 1,2-dioleyl-sn-glycero-3-phosphocoline and dimethyl dioctadecyl ammonium bromide (DODAB) and coated by alternated layers of the sodium salt of poly(4-styrenesulfonate) (PSS) and poly(allylamine) (PAH) as polyanions and polycations, respectively. The results show that the zeta potential of the liposomes was not very sensitive to the mole fraction of DODAB in the membrane, XD, in the range 0.3 ≤ XD ≤ 0.8. We were able to coat the liposomes with up to four polymer bilayers. The growth of the capsule size was followed by dynamic light scattering, and in some cases, by cryo-transmission electron microscopy, with good agreement between both techniques. The thickness of the layers, measured from the hydrodynamic radius of the coated liposome, depends on the polyelectrolyte used, so that the PSS layers adopt a much more packaged conformation than the PAH layers. An interesting finding is that the PSS amount needed to reach the isoelectric point of the capsules increases linearly with the charge density of the bare liposomes, whereas the amount of PAH does not depend on it. As expected, the preparation of the multilayers has to be done in such a way that when the system is close to the isoelectric point, the capsules do not aggregate. For this, we dropped the polyelectrolyte solution quickly, stirred it fast, and used dilute liposome suspensions. The method is very flexible and not limited to liposomes or polyelectrolyte multilayers; also, coatings containing charged nanoparticles can be easily made. Once the liposomes have been coated, lipids can be easily eliminated, giving rise to polyelectrolyte nanocapsules (polyelectrosomes) with potential applications as drug delivery platforms.
Fil: Ruano, Marta. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Mateos Maroto, Ana. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Ortega, Francisco. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Ritacco, Hernán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Física del Sur. Universidad Nacional del Sur. Departamento de Física. Instituto de Física del Sur; Argentina
Fil: Fernández Rubio, José Emilio. Universidad Complutense de Madrid; España
Fil: Guzmán, Eduardo. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Rubio, Ramón G.. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España - Materia
-
LIPOSOMES
LAYER BY LAYER
CAPSULES
NANOTECHNOLOGY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/204575
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Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged LiposomesRuano, MartaMateos Maroto, AnaOrtega, FranciscoRitacco, Hernán AlejandroFernández Rubio, José EmilioGuzmán, EduardoRubio, Ramón G.LIPOSOMESLAYER BY LAYERCAPSULESNANOTECHNOLOGYhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1This work presents a simple methodology for coating small unilamellar liposomes bearing different degrees of positive charge with polyelectrolyte multilayers using the sequential layer-by-layer deposition method. The liposomes were made of mixtures of 1,2-dioleyl-sn-glycero-3-phosphocoline and dimethyl dioctadecyl ammonium bromide (DODAB) and coated by alternated layers of the sodium salt of poly(4-styrenesulfonate) (PSS) and poly(allylamine) (PAH) as polyanions and polycations, respectively. The results show that the zeta potential of the liposomes was not very sensitive to the mole fraction of DODAB in the membrane, XD, in the range 0.3 ≤ XD ≤ 0.8. We were able to coat the liposomes with up to four polymer bilayers. The growth of the capsule size was followed by dynamic light scattering, and in some cases, by cryo-transmission electron microscopy, with good agreement between both techniques. The thickness of the layers, measured from the hydrodynamic radius of the coated liposome, depends on the polyelectrolyte used, so that the PSS layers adopt a much more packaged conformation than the PAH layers. An interesting finding is that the PSS amount needed to reach the isoelectric point of the capsules increases linearly with the charge density of the bare liposomes, whereas the amount of PAH does not depend on it. As expected, the preparation of the multilayers has to be done in such a way that when the system is close to the isoelectric point, the capsules do not aggregate. For this, we dropped the polyelectrolyte solution quickly, stirred it fast, and used dilute liposome suspensions. The method is very flexible and not limited to liposomes or polyelectrolyte multilayers; also, coatings containing charged nanoparticles can be easily made. Once the liposomes have been coated, lipids can be easily eliminated, giving rise to polyelectrolyte nanocapsules (polyelectrosomes) with potential applications as drug delivery platforms.Fil: Ruano, Marta. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Mateos Maroto, Ana. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Ortega, Francisco. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Ritacco, Hernán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Física del Sur. Universidad Nacional del Sur. Departamento de Física. Instituto de Física del Sur; ArgentinaFil: Fernández Rubio, José Emilio. Universidad Complutense de Madrid; EspañaFil: Guzmán, Eduardo. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Rubio, Ramón G.. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaAmerican Chemical Society2021-05-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/204575Ruano, Marta; Mateos Maroto, Ana; Ortega, Francisco; Ritacco, Hernán Alejandro; Fernández Rubio, José Emilio; et al.; Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes; American Chemical Society; Langmuir; 37; 20; 4-5-2021; 6189-62000743-7463CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/acs.langmuir.1c00341info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/pdf/10.1021/acs.langmuir.1c00341info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:21Zoai:ri.conicet.gov.ar:11336/204575instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:21.903CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes |
title |
Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes |
spellingShingle |
Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes Ruano, Marta LIPOSOMES LAYER BY LAYER CAPSULES NANOTECHNOLOGY |
title_short |
Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes |
title_full |
Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes |
title_fullStr |
Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes |
title_full_unstemmed |
Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes |
title_sort |
Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes |
dc.creator.none.fl_str_mv |
Ruano, Marta Mateos Maroto, Ana Ortega, Francisco Ritacco, Hernán Alejandro Fernández Rubio, José Emilio Guzmán, Eduardo Rubio, Ramón G. |
author |
Ruano, Marta |
author_facet |
Ruano, Marta Mateos Maroto, Ana Ortega, Francisco Ritacco, Hernán Alejandro Fernández Rubio, José Emilio Guzmán, Eduardo Rubio, Ramón G. |
author_role |
author |
author2 |
Mateos Maroto, Ana Ortega, Francisco Ritacco, Hernán Alejandro Fernández Rubio, José Emilio Guzmán, Eduardo Rubio, Ramón G. |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
LIPOSOMES LAYER BY LAYER CAPSULES NANOTECHNOLOGY |
topic |
LIPOSOMES LAYER BY LAYER CAPSULES NANOTECHNOLOGY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.3 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
This work presents a simple methodology for coating small unilamellar liposomes bearing different degrees of positive charge with polyelectrolyte multilayers using the sequential layer-by-layer deposition method. The liposomes were made of mixtures of 1,2-dioleyl-sn-glycero-3-phosphocoline and dimethyl dioctadecyl ammonium bromide (DODAB) and coated by alternated layers of the sodium salt of poly(4-styrenesulfonate) (PSS) and poly(allylamine) (PAH) as polyanions and polycations, respectively. The results show that the zeta potential of the liposomes was not very sensitive to the mole fraction of DODAB in the membrane, XD, in the range 0.3 ≤ XD ≤ 0.8. We were able to coat the liposomes with up to four polymer bilayers. The growth of the capsule size was followed by dynamic light scattering, and in some cases, by cryo-transmission electron microscopy, with good agreement between both techniques. The thickness of the layers, measured from the hydrodynamic radius of the coated liposome, depends on the polyelectrolyte used, so that the PSS layers adopt a much more packaged conformation than the PAH layers. An interesting finding is that the PSS amount needed to reach the isoelectric point of the capsules increases linearly with the charge density of the bare liposomes, whereas the amount of PAH does not depend on it. As expected, the preparation of the multilayers has to be done in such a way that when the system is close to the isoelectric point, the capsules do not aggregate. For this, we dropped the polyelectrolyte solution quickly, stirred it fast, and used dilute liposome suspensions. The method is very flexible and not limited to liposomes or polyelectrolyte multilayers; also, coatings containing charged nanoparticles can be easily made. Once the liposomes have been coated, lipids can be easily eliminated, giving rise to polyelectrolyte nanocapsules (polyelectrosomes) with potential applications as drug delivery platforms. Fil: Ruano, Marta. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España Fil: Mateos Maroto, Ana. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España Fil: Ortega, Francisco. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España Fil: Ritacco, Hernán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Física del Sur. Universidad Nacional del Sur. Departamento de Física. Instituto de Física del Sur; Argentina Fil: Fernández Rubio, José Emilio. Universidad Complutense de Madrid; España Fil: Guzmán, Eduardo. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España Fil: Rubio, Ramón G.. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España |
description |
This work presents a simple methodology for coating small unilamellar liposomes bearing different degrees of positive charge with polyelectrolyte multilayers using the sequential layer-by-layer deposition method. The liposomes were made of mixtures of 1,2-dioleyl-sn-glycero-3-phosphocoline and dimethyl dioctadecyl ammonium bromide (DODAB) and coated by alternated layers of the sodium salt of poly(4-styrenesulfonate) (PSS) and poly(allylamine) (PAH) as polyanions and polycations, respectively. The results show that the zeta potential of the liposomes was not very sensitive to the mole fraction of DODAB in the membrane, XD, in the range 0.3 ≤ XD ≤ 0.8. We were able to coat the liposomes with up to four polymer bilayers. The growth of the capsule size was followed by dynamic light scattering, and in some cases, by cryo-transmission electron microscopy, with good agreement between both techniques. The thickness of the layers, measured from the hydrodynamic radius of the coated liposome, depends on the polyelectrolyte used, so that the PSS layers adopt a much more packaged conformation than the PAH layers. An interesting finding is that the PSS amount needed to reach the isoelectric point of the capsules increases linearly with the charge density of the bare liposomes, whereas the amount of PAH does not depend on it. As expected, the preparation of the multilayers has to be done in such a way that when the system is close to the isoelectric point, the capsules do not aggregate. For this, we dropped the polyelectrolyte solution quickly, stirred it fast, and used dilute liposome suspensions. The method is very flexible and not limited to liposomes or polyelectrolyte multilayers; also, coatings containing charged nanoparticles can be easily made. Once the liposomes have been coated, lipids can be easily eliminated, giving rise to polyelectrolyte nanocapsules (polyelectrosomes) with potential applications as drug delivery platforms. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/204575 Ruano, Marta; Mateos Maroto, Ana; Ortega, Francisco; Ritacco, Hernán Alejandro; Fernández Rubio, José Emilio; et al.; Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes; American Chemical Society; Langmuir; 37; 20; 4-5-2021; 6189-6200 0743-7463 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/204575 |
identifier_str_mv |
Ruano, Marta; Mateos Maroto, Ana; Ortega, Francisco; Ritacco, Hernán Alejandro; Fernández Rubio, José Emilio; et al.; Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes; American Chemical Society; Langmuir; 37; 20; 4-5-2021; 6189-6200 0743-7463 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.langmuir.1c00341 info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/pdf/10.1021/acs.langmuir.1c00341 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614287362883584 |
score |
13.070432 |