Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes

Autores
Ruano, Marta; Mateos Maroto, Ana; Ortega, Francisco; Ritacco, Hernán Alejandro; Fernández Rubio, José Emilio; Guzmán, Eduardo; Rubio, Ramón G.
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work presents a simple methodology for coating small unilamellar liposomes bearing different degrees of positive charge with polyelectrolyte multilayers using the sequential layer-by-layer deposition method. The liposomes were made of mixtures of 1,2-dioleyl-sn-glycero-3-phosphocoline and dimethyl dioctadecyl ammonium bromide (DODAB) and coated by alternated layers of the sodium salt of poly(4-styrenesulfonate) (PSS) and poly(allylamine) (PAH) as polyanions and polycations, respectively. The results show that the zeta potential of the liposomes was not very sensitive to the mole fraction of DODAB in the membrane, XD, in the range 0.3 ≤ XD ≤ 0.8. We were able to coat the liposomes with up to four polymer bilayers. The growth of the capsule size was followed by dynamic light scattering, and in some cases, by cryo-transmission electron microscopy, with good agreement between both techniques. The thickness of the layers, measured from the hydrodynamic radius of the coated liposome, depends on the polyelectrolyte used, so that the PSS layers adopt a much more packaged conformation than the PAH layers. An interesting finding is that the PSS amount needed to reach the isoelectric point of the capsules increases linearly with the charge density of the bare liposomes, whereas the amount of PAH does not depend on it. As expected, the preparation of the multilayers has to be done in such a way that when the system is close to the isoelectric point, the capsules do not aggregate. For this, we dropped the polyelectrolyte solution quickly, stirred it fast, and used dilute liposome suspensions. The method is very flexible and not limited to liposomes or polyelectrolyte multilayers; also, coatings containing charged nanoparticles can be easily made. Once the liposomes have been coated, lipids can be easily eliminated, giving rise to polyelectrolyte nanocapsules (polyelectrosomes) with potential applications as drug delivery platforms.
Fil: Ruano, Marta. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Mateos Maroto, Ana. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Ortega, Francisco. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Ritacco, Hernán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Física del Sur. Universidad Nacional del Sur. Departamento de Física. Instituto de Física del Sur; Argentina
Fil: Fernández Rubio, José Emilio. Universidad Complutense de Madrid; España
Fil: Guzmán, Eduardo. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Rubio, Ramón G.. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Materia
LIPOSOMES
LAYER BY LAYER
CAPSULES
NANOTECHNOLOGY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/204575
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/204575
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged LiposomesRuano, MartaMateos Maroto, AnaOrtega, FranciscoRitacco, Hernán AlejandroFernández Rubio, José EmilioGuzmán, EduardoRubio, Ramón G.LIPOSOMESLAYER BY LAYERCAPSULESNANOTECHNOLOGYhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1This work presents a simple methodology for coating small unilamellar liposomes bearing different degrees of positive charge with polyelectrolyte multilayers using the sequential layer-by-layer deposition method. The liposomes were made of mixtures of 1,2-dioleyl-sn-glycero-3-phosphocoline and dimethyl dioctadecyl ammonium bromide (DODAB) and coated by alternated layers of the sodium salt of poly(4-styrenesulfonate) (PSS) and poly(allylamine) (PAH) as polyanions and polycations, respectively. The results show that the zeta potential of the liposomes was not very sensitive to the mole fraction of DODAB in the membrane, XD, in the range 0.3 ≤ XD ≤ 0.8. We were able to coat the liposomes with up to four polymer bilayers. The growth of the capsule size was followed by dynamic light scattering, and in some cases, by cryo-transmission electron microscopy, with good agreement between both techniques. The thickness of the layers, measured from the hydrodynamic radius of the coated liposome, depends on the polyelectrolyte used, so that the PSS layers adopt a much more packaged conformation than the PAH layers. An interesting finding is that the PSS amount needed to reach the isoelectric point of the capsules increases linearly with the charge density of the bare liposomes, whereas the amount of PAH does not depend on it. As expected, the preparation of the multilayers has to be done in such a way that when the system is close to the isoelectric point, the capsules do not aggregate. For this, we dropped the polyelectrolyte solution quickly, stirred it fast, and used dilute liposome suspensions. The method is very flexible and not limited to liposomes or polyelectrolyte multilayers; also, coatings containing charged nanoparticles can be easily made. Once the liposomes have been coated, lipids can be easily eliminated, giving rise to polyelectrolyte nanocapsules (polyelectrosomes) with potential applications as drug delivery platforms.Fil: Ruano, Marta. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Mateos Maroto, Ana. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Ortega, Francisco. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Ritacco, Hernán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Física del Sur. Universidad Nacional del Sur. Departamento de Física. Instituto de Física del Sur; ArgentinaFil: Fernández Rubio, José Emilio. Universidad Complutense de Madrid; EspañaFil: Guzmán, Eduardo. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Rubio, Ramón G.. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaAmerican Chemical Society2021-05-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/204575Ruano, Marta; Mateos Maroto, Ana; Ortega, Francisco; Ritacco, Hernán Alejandro; Fernández Rubio, José Emilio; et al.; Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes; American Chemical Society; Langmuir; 37; 20; 4-5-2021; 6189-62000743-7463CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/acs.langmuir.1c00341info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/pdf/10.1021/acs.langmuir.1c00341info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:00:43Zoai:ri.conicet.gov.ar:11336/204575instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:00:43.881CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes
title Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes
spellingShingle Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes
Ruano, Marta
LIPOSOMES
LAYER BY LAYER
CAPSULES
NANOTECHNOLOGY
title_short Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes
title_full Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes
title_fullStr Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes
title_full_unstemmed Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes
title_sort Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes
dc.creator.none.fl_str_mv Ruano, Marta
Mateos Maroto, Ana
Ortega, Francisco
Ritacco, Hernán Alejandro
Fernández Rubio, José Emilio
Guzmán, Eduardo
Rubio, Ramón G.
author Ruano, Marta
author_facet Ruano, Marta
Mateos Maroto, Ana
Ortega, Francisco
Ritacco, Hernán Alejandro
Fernández Rubio, José Emilio
Guzmán, Eduardo
Rubio, Ramón G.
author_role author
author2 Mateos Maroto, Ana
Ortega, Francisco
Ritacco, Hernán Alejandro
Fernández Rubio, José Emilio
Guzmán, Eduardo
Rubio, Ramón G.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv LIPOSOMES
LAYER BY LAYER
CAPSULES
NANOTECHNOLOGY
topic LIPOSOMES
LAYER BY LAYER
CAPSULES
NANOTECHNOLOGY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This work presents a simple methodology for coating small unilamellar liposomes bearing different degrees of positive charge with polyelectrolyte multilayers using the sequential layer-by-layer deposition method. The liposomes were made of mixtures of 1,2-dioleyl-sn-glycero-3-phosphocoline and dimethyl dioctadecyl ammonium bromide (DODAB) and coated by alternated layers of the sodium salt of poly(4-styrenesulfonate) (PSS) and poly(allylamine) (PAH) as polyanions and polycations, respectively. The results show that the zeta potential of the liposomes was not very sensitive to the mole fraction of DODAB in the membrane, XD, in the range 0.3 ≤ XD ≤ 0.8. We were able to coat the liposomes with up to four polymer bilayers. The growth of the capsule size was followed by dynamic light scattering, and in some cases, by cryo-transmission electron microscopy, with good agreement between both techniques. The thickness of the layers, measured from the hydrodynamic radius of the coated liposome, depends on the polyelectrolyte used, so that the PSS layers adopt a much more packaged conformation than the PAH layers. An interesting finding is that the PSS amount needed to reach the isoelectric point of the capsules increases linearly with the charge density of the bare liposomes, whereas the amount of PAH does not depend on it. As expected, the preparation of the multilayers has to be done in such a way that when the system is close to the isoelectric point, the capsules do not aggregate. For this, we dropped the polyelectrolyte solution quickly, stirred it fast, and used dilute liposome suspensions. The method is very flexible and not limited to liposomes or polyelectrolyte multilayers; also, coatings containing charged nanoparticles can be easily made. Once the liposomes have been coated, lipids can be easily eliminated, giving rise to polyelectrolyte nanocapsules (polyelectrosomes) with potential applications as drug delivery platforms.
Fil: Ruano, Marta. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Mateos Maroto, Ana. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Ortega, Francisco. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Ritacco, Hernán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Física del Sur. Universidad Nacional del Sur. Departamento de Física. Instituto de Física del Sur; Argentina
Fil: Fernández Rubio, José Emilio. Universidad Complutense de Madrid; España
Fil: Guzmán, Eduardo. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Rubio, Ramón G.. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
description This work presents a simple methodology for coating small unilamellar liposomes bearing different degrees of positive charge with polyelectrolyte multilayers using the sequential layer-by-layer deposition method. The liposomes were made of mixtures of 1,2-dioleyl-sn-glycero-3-phosphocoline and dimethyl dioctadecyl ammonium bromide (DODAB) and coated by alternated layers of the sodium salt of poly(4-styrenesulfonate) (PSS) and poly(allylamine) (PAH) as polyanions and polycations, respectively. The results show that the zeta potential of the liposomes was not very sensitive to the mole fraction of DODAB in the membrane, XD, in the range 0.3 ≤ XD ≤ 0.8. We were able to coat the liposomes with up to four polymer bilayers. The growth of the capsule size was followed by dynamic light scattering, and in some cases, by cryo-transmission electron microscopy, with good agreement between both techniques. The thickness of the layers, measured from the hydrodynamic radius of the coated liposome, depends on the polyelectrolyte used, so that the PSS layers adopt a much more packaged conformation than the PAH layers. An interesting finding is that the PSS amount needed to reach the isoelectric point of the capsules increases linearly with the charge density of the bare liposomes, whereas the amount of PAH does not depend on it. As expected, the preparation of the multilayers has to be done in such a way that when the system is close to the isoelectric point, the capsules do not aggregate. For this, we dropped the polyelectrolyte solution quickly, stirred it fast, and used dilute liposome suspensions. The method is very flexible and not limited to liposomes or polyelectrolyte multilayers; also, coatings containing charged nanoparticles can be easily made. Once the liposomes have been coated, lipids can be easily eliminated, giving rise to polyelectrolyte nanocapsules (polyelectrosomes) with potential applications as drug delivery platforms.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/204575
Ruano, Marta; Mateos Maroto, Ana; Ortega, Francisco; Ritacco, Hernán Alejandro; Fernández Rubio, José Emilio; et al.; Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes; American Chemical Society; Langmuir; 37; 20; 4-5-2021; 6189-6200
0743-7463
CONICET Digital
CONICET
url http://hdl.handle.net/11336/204575
identifier_str_mv Ruano, Marta; Mateos Maroto, Ana; Ortega, Francisco; Ritacco, Hernán Alejandro; Fernández Rubio, José Emilio; et al.; Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes; American Chemical Society; Langmuir; 37; 20; 4-5-2021; 6189-6200
0743-7463
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.langmuir.1c00341
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/pdf/10.1021/acs.langmuir.1c00341
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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