Polarity studies of single polyelectrolyte layers in polyelectrolyte multilayers probed by steady state and life time doxorubicin fluorescence

Autores
Martinelli, Hernan; Tasca, Elisamaria; Andreozzi, Patrizia; Libertone, Sara; Ritacco, Hernán Alejandro; Giustini, Mauro; Moya, Sergio Enrique
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hypothesis: Polarity in polyelectrolyte multilayers (PEMs) may vary from the inner to the top layers of the film as the charge compensation of the layers is more effective inside the PEMs than in outer layers. Doxorubicin hydrochloride (DX) is used here to sense polarity at the single polyelectrolyte level inside PEMS. Experimental: DX is complexed electrostatically to a polyanion, either polystyrene sulfonate (PSS) or polyacrylic acid (PAA) and assembled at selected positions in a multilayer of the polyanion and polyally lamine hydrochloride (PAH) as polycation. Local polarity in the layer domain is evaluated through changes in the intensity ratio of the first to second band of spectra of DX (I1/I2 ratio) by steady state flu orescence, and by Lifetime fluorescence. Findings: PAH/PSS multilayers, show a polarity similar to water with DX/PSS as top layer, decreasing to I1/ I2 ratios similar to organic solvents as the number of polyelectrolyte layers assembled on top increases. For PAH/PAA multilayers, polarity values reflect a more polar environment than water when DX/PAA is the top layer, remaining unaltered by the assembly of polyelectrolyte layers on top. Results show that different polar environments may be present in a PEM when considering polarity at the single layer level.
Fil: Martinelli, Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Física del Sur. Universidad Nacional del Sur. Departamento de Física. Instituto de Física del Sur; Argentina. Centro de Investigación Cooperativa en Biomateriales; España
Fil: Tasca, Elisamaria. Centro de Investigación Cooperativa en Biomateriales; España. Università degli Studi di Roma "La Sapienza"; Italia
Fil: Andreozzi, Patrizia. Università degli Studi di Firenze; Dipartimento di Chimica “Ugo Schiff”; Italia. Centro de Investigación Cooperativa en Biomateriales; España
Fil: Libertone, Sara. Centro de Investigación Cooperativa en Biomateriales; España
Fil: Ritacco, Hernán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Física del Sur. Universidad Nacional del Sur. Departamento de Física. Instituto de Física del Sur; Argentina
Fil: Giustini, Mauro. Università degli Studi di Roma "La Sapienza"; Italia. Università degli Studi di Firenze; Dipartimento di Chimica “Ugo Schiff” ; Italia
Fil: Moya, Sergio Enrique. Centro de Investigación Cooperativa en Biomateriales; España. Università degli Studi di Roma "La Sapienza"; Italia
Materia
DOXORUBICIN
FLUORESCENCE LIFETIME
LAYER BY LAYER TECHNIQUE
POLARITY
POLYELECTROLYTE MULTILAYERS
Nivel de accesibilidad
acceso embargado
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/171774
Acceder
id CONICETDig_e5dc00293188a231e47f19ae9c12e5d2
oai_identifier_str oai:ri.conicet.gov.ar:11336/171774
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Polarity studies of single polyelectrolyte layers in polyelectrolyte multilayers probed by steady state and life time doxorubicin fluorescenceMartinelli, HernanTasca, ElisamariaAndreozzi, PatriziaLibertone, SaraRitacco, Hernán AlejandroGiustini, MauroMoya, Sergio EnriqueDOXORUBICINFLUORESCENCE LIFETIMELAYER BY LAYER TECHNIQUEPOLARITYPOLYELECTROLYTE MULTILAYERShttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1Hypothesis: Polarity in polyelectrolyte multilayers (PEMs) may vary from the inner to the top layers of the film as the charge compensation of the layers is more effective inside the PEMs than in outer layers. Doxorubicin hydrochloride (DX) is used here to sense polarity at the single polyelectrolyte level inside PEMS. Experimental: DX is complexed electrostatically to a polyanion, either polystyrene sulfonate (PSS) or polyacrylic acid (PAA) and assembled at selected positions in a multilayer of the polyanion and polyally lamine hydrochloride (PAH) as polycation. Local polarity in the layer domain is evaluated through changes in the intensity ratio of the first to second band of spectra of DX (I1/I2 ratio) by steady state flu orescence, and by Lifetime fluorescence. Findings: PAH/PSS multilayers, show a polarity similar to water with DX/PSS as top layer, decreasing to I1/ I2 ratios similar to organic solvents as the number of polyelectrolyte layers assembled on top increases. For PAH/PAA multilayers, polarity values reflect a more polar environment than water when DX/PAA is the top layer, remaining unaltered by the assembly of polyelectrolyte layers on top. Results show that different polar environments may be present in a PEM when considering polarity at the single layer level.Fil: Martinelli, Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Física del Sur. Universidad Nacional del Sur. Departamento de Física. Instituto de Física del Sur; Argentina. Centro de Investigación Cooperativa en Biomateriales; EspañaFil: Tasca, Elisamaria. Centro de Investigación Cooperativa en Biomateriales; España. Università degli Studi di Roma "La Sapienza"; ItaliaFil: Andreozzi, Patrizia. Università degli Studi di Firenze; Dipartimento di Chimica “Ugo Schiff”; Italia. Centro de Investigación Cooperativa en Biomateriales; EspañaFil: Libertone, Sara. Centro de Investigación Cooperativa en Biomateriales; EspañaFil: Ritacco, Hernán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Física del Sur. Universidad Nacional del Sur. Departamento de Física. Instituto de Física del Sur; ArgentinaFil: Giustini, Mauro. Università degli Studi di Roma "La Sapienza"; Italia. Università degli Studi di Firenze; Dipartimento di Chimica “Ugo Schiff” ; ItaliaFil: Moya, Sergio Enrique. Centro de Investigación Cooperativa en Biomateriales; España. Università degli Studi di Roma "La Sapienza"; ItaliaElsevier2022-02info:eu-repo/date/embargoEnd/2023-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/171774Martinelli, Hernan; Tasca, Elisamaria; Andreozzi, Patrizia; Libertone, Sara; Ritacco, Hernán Alejandro; et al.; Polarity studies of single polyelectrolyte layers in polyelectrolyte multilayers probed by steady state and life time doxorubicin fluorescence; Elsevier; Journal of Colloid and Interface Science; 607; 1; 2-2022; 153-1620021-9797CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0021979721014417info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jcis.2021.08.207info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:44Zoai:ri.conicet.gov.ar:11336/171774instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:44.601CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Polarity studies of single polyelectrolyte layers in polyelectrolyte multilayers probed by steady state and life time doxorubicin fluorescence
title Polarity studies of single polyelectrolyte layers in polyelectrolyte multilayers probed by steady state and life time doxorubicin fluorescence
spellingShingle Polarity studies of single polyelectrolyte layers in polyelectrolyte multilayers probed by steady state and life time doxorubicin fluorescence
Martinelli, Hernan
DOXORUBICIN
FLUORESCENCE LIFETIME
LAYER BY LAYER TECHNIQUE
POLARITY
POLYELECTROLYTE MULTILAYERS
title_short Polarity studies of single polyelectrolyte layers in polyelectrolyte multilayers probed by steady state and life time doxorubicin fluorescence
title_full Polarity studies of single polyelectrolyte layers in polyelectrolyte multilayers probed by steady state and life time doxorubicin fluorescence
title_fullStr Polarity studies of single polyelectrolyte layers in polyelectrolyte multilayers probed by steady state and life time doxorubicin fluorescence
title_full_unstemmed Polarity studies of single polyelectrolyte layers in polyelectrolyte multilayers probed by steady state and life time doxorubicin fluorescence
title_sort Polarity studies of single polyelectrolyte layers in polyelectrolyte multilayers probed by steady state and life time doxorubicin fluorescence
dc.creator.none.fl_str_mv Martinelli, Hernan
Tasca, Elisamaria
Andreozzi, Patrizia
Libertone, Sara
Ritacco, Hernán Alejandro
Giustini, Mauro
Moya, Sergio Enrique
author Martinelli, Hernan
author_facet Martinelli, Hernan
Tasca, Elisamaria
Andreozzi, Patrizia
Libertone, Sara
Ritacco, Hernán Alejandro
Giustini, Mauro
Moya, Sergio Enrique
author_role author
author2 Tasca, Elisamaria
Andreozzi, Patrizia
Libertone, Sara
Ritacco, Hernán Alejandro
Giustini, Mauro
Moya, Sergio Enrique
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv DOXORUBICIN
FLUORESCENCE LIFETIME
LAYER BY LAYER TECHNIQUE
POLARITY
POLYELECTROLYTE MULTILAYERS
topic DOXORUBICIN
FLUORESCENCE LIFETIME
LAYER BY LAYER TECHNIQUE
POLARITY
POLYELECTROLYTE MULTILAYERS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Hypothesis: Polarity in polyelectrolyte multilayers (PEMs) may vary from the inner to the top layers of the film as the charge compensation of the layers is more effective inside the PEMs than in outer layers. Doxorubicin hydrochloride (DX) is used here to sense polarity at the single polyelectrolyte level inside PEMS. Experimental: DX is complexed electrostatically to a polyanion, either polystyrene sulfonate (PSS) or polyacrylic acid (PAA) and assembled at selected positions in a multilayer of the polyanion and polyally lamine hydrochloride (PAH) as polycation. Local polarity in the layer domain is evaluated through changes in the intensity ratio of the first to second band of spectra of DX (I1/I2 ratio) by steady state flu orescence, and by Lifetime fluorescence. Findings: PAH/PSS multilayers, show a polarity similar to water with DX/PSS as top layer, decreasing to I1/ I2 ratios similar to organic solvents as the number of polyelectrolyte layers assembled on top increases. For PAH/PAA multilayers, polarity values reflect a more polar environment than water when DX/PAA is the top layer, remaining unaltered by the assembly of polyelectrolyte layers on top. Results show that different polar environments may be present in a PEM when considering polarity at the single layer level.
Fil: Martinelli, Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Física del Sur. Universidad Nacional del Sur. Departamento de Física. Instituto de Física del Sur; Argentina. Centro de Investigación Cooperativa en Biomateriales; España
Fil: Tasca, Elisamaria. Centro de Investigación Cooperativa en Biomateriales; España. Università degli Studi di Roma "La Sapienza"; Italia
Fil: Andreozzi, Patrizia. Università degli Studi di Firenze; Dipartimento di Chimica “Ugo Schiff”; Italia. Centro de Investigación Cooperativa en Biomateriales; España
Fil: Libertone, Sara. Centro de Investigación Cooperativa en Biomateriales; España
Fil: Ritacco, Hernán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Física del Sur. Universidad Nacional del Sur. Departamento de Física. Instituto de Física del Sur; Argentina
Fil: Giustini, Mauro. Università degli Studi di Roma "La Sapienza"; Italia. Università degli Studi di Firenze; Dipartimento di Chimica “Ugo Schiff” ; Italia
Fil: Moya, Sergio Enrique. Centro de Investigación Cooperativa en Biomateriales; España. Università degli Studi di Roma "La Sapienza"; Italia
description Hypothesis: Polarity in polyelectrolyte multilayers (PEMs) may vary from the inner to the top layers of the film as the charge compensation of the layers is more effective inside the PEMs than in outer layers. Doxorubicin hydrochloride (DX) is used here to sense polarity at the single polyelectrolyte level inside PEMS. Experimental: DX is complexed electrostatically to a polyanion, either polystyrene sulfonate (PSS) or polyacrylic acid (PAA) and assembled at selected positions in a multilayer of the polyanion and polyally lamine hydrochloride (PAH) as polycation. Local polarity in the layer domain is evaluated through changes in the intensity ratio of the first to second band of spectra of DX (I1/I2 ratio) by steady state flu orescence, and by Lifetime fluorescence. Findings: PAH/PSS multilayers, show a polarity similar to water with DX/PSS as top layer, decreasing to I1/ I2 ratios similar to organic solvents as the number of polyelectrolyte layers assembled on top increases. For PAH/PAA multilayers, polarity values reflect a more polar environment than water when DX/PAA is the top layer, remaining unaltered by the assembly of polyelectrolyte layers on top. Results show that different polar environments may be present in a PEM when considering polarity at the single layer level.
publishDate 2022
dc.date.none.fl_str_mv 2022-02
info:eu-repo/date/embargoEnd/2023-02-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/171774
Martinelli, Hernan; Tasca, Elisamaria; Andreozzi, Patrizia; Libertone, Sara; Ritacco, Hernán Alejandro; et al.; Polarity studies of single polyelectrolyte layers in polyelectrolyte multilayers probed by steady state and life time doxorubicin fluorescence; Elsevier; Journal of Colloid and Interface Science; 607; 1; 2-2022; 153-162
0021-9797
CONICET Digital
CONICET
url http://hdl.handle.net/11336/171774
identifier_str_mv Martinelli, Hernan; Tasca, Elisamaria; Andreozzi, Patrizia; Libertone, Sara; Ritacco, Hernán Alejandro; et al.; Polarity studies of single polyelectrolyte layers in polyelectrolyte multilayers probed by steady state and life time doxorubicin fluorescence; Elsevier; Journal of Colloid and Interface Science; 607; 1; 2-2022; 153-162
0021-9797
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0021979721014417
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jcis.2021.08.207
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv embargoedAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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