Genetic dissection of vitamin e biosynthesis in tomato

Autores
Almeida, Juliana; Quadrana, Leandro Daniel; Asis, Ramón; Setta, Nathalia; De Godoy, Fabiana; Bermúdez, Luisa; Otaiza González, Santiago Nicolás; Corrêa Da Silva, Junia V.; Fernie, Alisdair R.; Carrari, Fernando Oscar; Rossi, Magdalena
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Vegetables are critical for human health as they are a source of multiple vitamins including vitamin E (VTE). In plants, the synthesis of VTE compounds, tocopherol and tocotrienol, derives from precursors of the shikimate and methylerythritol phosphate pathways. Quantitative trait loci (QTL) for α-tocopherol content in ripe fruit have previously been determined in an Solanum pennellii tomato introgression line population. In this work, variations of tocopherol isoforms (α, β, γ, and δ) in ripe fruits of these lines were studied. In parallel all tomato genes structurally associated with VTE biosynthesis were identified and mapped. Previously identified VTE QTL on chromosomes 6 and 9 were confirmed whilst novel ones were identified on chromosomes 7 and 8. Integrated analysis at the metabolic, genetic and genomic levels allowed us to propose 16 candidate loci putatively affecting tocopherol content in tomato. A comparative analysis revealed polymorphisms at nucleotide and amino acid levels between Solanum lycopersicum and S. pennellii candidate alleles. Moreover, evolutionary analyses showed the presence of codons evolving under both neutral and positive selection, which may explain the phenotypic differences between species. These data represent an important step in understanding the genetic determinants of VTE natural variation in tomato fruit and as such in the ability to improve the content of this important nutriceutical.
Fil: Almeida, Juliana. Universidade de Sao Paulo; Brasil
Fil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Setta, Nathalia. Universidade de Sao Paulo; Brasil
Fil: De Godoy, Fabiana. Universidade de Sao Paulo; Brasil
Fil: Bermúdez, Luisa. Universidade de Sao Paulo; Brasil
Fil: Otaiza González, Santiago Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Corrêa Da Silva, Junia V.. Universidade de Sao Paulo; Brasil
Fil: Fernie, Alisdair R.. No especifíca;
Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rossi, Magdalena. Universidade de Sao Paulo; Brasil
Materia
FRUIT METABOLISM
SOLANUM PENNELLII
TOCOPHEROL
TOMATO
VITAMIN E
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/192846
Acceder
id CONICETDig_cc27d2cfb691a3d94e7db60c8774884c
oai_identifier_str oai:ri.conicet.gov.ar:11336/192846
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genetic dissection of vitamin e biosynthesis in tomatoAlmeida, JulianaQuadrana, Leandro DanielAsis, RamónSetta, NathaliaDe Godoy, FabianaBermúdez, LuisaOtaiza González, Santiago NicolásCorrêa Da Silva, Junia V.Fernie, Alisdair R.Carrari, Fernando OscarRossi, MagdalenaFRUIT METABOLISMSOLANUM PENNELLIITOCOPHEROLTOMATOVITAMIN Ehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Vegetables are critical for human health as they are a source of multiple vitamins including vitamin E (VTE). In plants, the synthesis of VTE compounds, tocopherol and tocotrienol, derives from precursors of the shikimate and methylerythritol phosphate pathways. Quantitative trait loci (QTL) for α-tocopherol content in ripe fruit have previously been determined in an Solanum pennellii tomato introgression line population. In this work, variations of tocopherol isoforms (α, β, γ, and δ) in ripe fruits of these lines were studied. In parallel all tomato genes structurally associated with VTE biosynthesis were identified and mapped. Previously identified VTE QTL on chromosomes 6 and 9 were confirmed whilst novel ones were identified on chromosomes 7 and 8. Integrated analysis at the metabolic, genetic and genomic levels allowed us to propose 16 candidate loci putatively affecting tocopherol content in tomato. A comparative analysis revealed polymorphisms at nucleotide and amino acid levels between Solanum lycopersicum and S. pennellii candidate alleles. Moreover, evolutionary analyses showed the presence of codons evolving under both neutral and positive selection, which may explain the phenotypic differences between species. These data represent an important step in understanding the genetic determinants of VTE natural variation in tomato fruit and as such in the ability to improve the content of this important nutriceutical.Fil: Almeida, Juliana. Universidade de Sao Paulo; BrasilFil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Setta, Nathalia. Universidade de Sao Paulo; BrasilFil: De Godoy, Fabiana. Universidade de Sao Paulo; BrasilFil: Bermúdez, Luisa. Universidade de Sao Paulo; BrasilFil: Otaiza González, Santiago Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Corrêa Da Silva, Junia V.. Universidade de Sao Paulo; BrasilFil: Fernie, Alisdair R.. No especifíca;Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rossi, Magdalena. Universidade de Sao Paulo; BrasilOxford University Press2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192846Almeida, Juliana; Quadrana, Leandro Daniel; Asis, Ramón; Setta, Nathalia; De Godoy, Fabiana; et al.; Genetic dissection of vitamin e biosynthesis in tomato; Oxford University Press; Journal of Experimental Botany; 62; 11; 3-2011; 3781-37980022-0957CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article/62/11/3781/501890info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/err055info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:41Zoai:ri.conicet.gov.ar:11336/192846instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:41.725CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genetic dissection of vitamin e biosynthesis in tomato
title Genetic dissection of vitamin e biosynthesis in tomato
spellingShingle Genetic dissection of vitamin e biosynthesis in tomato
Almeida, Juliana
FRUIT METABOLISM
SOLANUM PENNELLII
TOCOPHEROL
TOMATO
VITAMIN E
title_short Genetic dissection of vitamin e biosynthesis in tomato
title_full Genetic dissection of vitamin e biosynthesis in tomato
title_fullStr Genetic dissection of vitamin e biosynthesis in tomato
title_full_unstemmed Genetic dissection of vitamin e biosynthesis in tomato
title_sort Genetic dissection of vitamin e biosynthesis in tomato
dc.creator.none.fl_str_mv Almeida, Juliana
Quadrana, Leandro Daniel
Asis, Ramón
Setta, Nathalia
De Godoy, Fabiana
Bermúdez, Luisa
Otaiza González, Santiago Nicolás
Corrêa Da Silva, Junia V.
Fernie, Alisdair R.
Carrari, Fernando Oscar
Rossi, Magdalena
author Almeida, Juliana
author_facet Almeida, Juliana
Quadrana, Leandro Daniel
Asis, Ramón
Setta, Nathalia
De Godoy, Fabiana
Bermúdez, Luisa
Otaiza González, Santiago Nicolás
Corrêa Da Silva, Junia V.
Fernie, Alisdair R.
Carrari, Fernando Oscar
Rossi, Magdalena
author_role author
author2 Quadrana, Leandro Daniel
Asis, Ramón
Setta, Nathalia
De Godoy, Fabiana
Bermúdez, Luisa
Otaiza González, Santiago Nicolás
Corrêa Da Silva, Junia V.
Fernie, Alisdair R.
Carrari, Fernando Oscar
Rossi, Magdalena
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FRUIT METABOLISM
SOLANUM PENNELLII
TOCOPHEROL
TOMATO
VITAMIN E
topic FRUIT METABOLISM
SOLANUM PENNELLII
TOCOPHEROL
TOMATO
VITAMIN E
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Vegetables are critical for human health as they are a source of multiple vitamins including vitamin E (VTE). In plants, the synthesis of VTE compounds, tocopherol and tocotrienol, derives from precursors of the shikimate and methylerythritol phosphate pathways. Quantitative trait loci (QTL) for α-tocopherol content in ripe fruit have previously been determined in an Solanum pennellii tomato introgression line population. In this work, variations of tocopherol isoforms (α, β, γ, and δ) in ripe fruits of these lines were studied. In parallel all tomato genes structurally associated with VTE biosynthesis were identified and mapped. Previously identified VTE QTL on chromosomes 6 and 9 were confirmed whilst novel ones were identified on chromosomes 7 and 8. Integrated analysis at the metabolic, genetic and genomic levels allowed us to propose 16 candidate loci putatively affecting tocopherol content in tomato. A comparative analysis revealed polymorphisms at nucleotide and amino acid levels between Solanum lycopersicum and S. pennellii candidate alleles. Moreover, evolutionary analyses showed the presence of codons evolving under both neutral and positive selection, which may explain the phenotypic differences between species. These data represent an important step in understanding the genetic determinants of VTE natural variation in tomato fruit and as such in the ability to improve the content of this important nutriceutical.
Fil: Almeida, Juliana. Universidade de Sao Paulo; Brasil
Fil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Setta, Nathalia. Universidade de Sao Paulo; Brasil
Fil: De Godoy, Fabiana. Universidade de Sao Paulo; Brasil
Fil: Bermúdez, Luisa. Universidade de Sao Paulo; Brasil
Fil: Otaiza González, Santiago Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Corrêa Da Silva, Junia V.. Universidade de Sao Paulo; Brasil
Fil: Fernie, Alisdair R.. No especifíca;
Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rossi, Magdalena. Universidade de Sao Paulo; Brasil
description Vegetables are critical for human health as they are a source of multiple vitamins including vitamin E (VTE). In plants, the synthesis of VTE compounds, tocopherol and tocotrienol, derives from precursors of the shikimate and methylerythritol phosphate pathways. Quantitative trait loci (QTL) for α-tocopherol content in ripe fruit have previously been determined in an Solanum pennellii tomato introgression line population. In this work, variations of tocopherol isoforms (α, β, γ, and δ) in ripe fruits of these lines were studied. In parallel all tomato genes structurally associated with VTE biosynthesis were identified and mapped. Previously identified VTE QTL on chromosomes 6 and 9 were confirmed whilst novel ones were identified on chromosomes 7 and 8. Integrated analysis at the metabolic, genetic and genomic levels allowed us to propose 16 candidate loci putatively affecting tocopherol content in tomato. A comparative analysis revealed polymorphisms at nucleotide and amino acid levels between Solanum lycopersicum and S. pennellii candidate alleles. Moreover, evolutionary analyses showed the presence of codons evolving under both neutral and positive selection, which may explain the phenotypic differences between species. These data represent an important step in understanding the genetic determinants of VTE natural variation in tomato fruit and as such in the ability to improve the content of this important nutriceutical.
publishDate 2011
dc.date.none.fl_str_mv 2011-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/192846
Almeida, Juliana; Quadrana, Leandro Daniel; Asis, Ramón; Setta, Nathalia; De Godoy, Fabiana; et al.; Genetic dissection of vitamin e biosynthesis in tomato; Oxford University Press; Journal of Experimental Botany; 62; 11; 3-2011; 3781-3798
0022-0957
CONICET Digital
CONICET
url http://hdl.handle.net/11336/192846
identifier_str_mv Almeida, Juliana; Quadrana, Leandro Daniel; Asis, Ramón; Setta, Nathalia; De Godoy, Fabiana; et al.; Genetic dissection of vitamin e biosynthesis in tomato; Oxford University Press; Journal of Experimental Botany; 62; 11; 3-2011; 3781-3798
0022-0957
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article/62/11/3781/501890
info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/err055
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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