Chitosan nanoparticles as potential immunomodulatory agents for bovine mastitis prevention

Autores
Rodriguez Berdini, Lucia; Breser, Maria Laura; Bohl, Luciana Paola; Monaco, Paula; Falcone, Ruben Dario; Porporatto, Carina
Año de publicación
2024
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Bovine mastitis is an inflammatory disease of the mammary gland tissue, primarily caused by bacterial infections. This condition is highly relevant in the dairy industry due to its impact on animal welfare and the significant economic losses associated with decreased milk yield and quality. Although antibiotic therapies are still the primary strategy for treating bovine mastitis, the increasing concern about antibiotic-resistant pathogens necessitates reduced antimicrobial use, prompting the search for alternative treatments, especially those derived from natural products. Given that dairy cattle with enhanced and balanced immune responses exhibit a lower incidence of the disease, immunostimulant treatments emerge as a promising alternative. Our research focuses on chitosan, a biocompatible, bioactive, and non-toxic polymer with well-documented antimicrobial and immunostimulant properties. Specifically, we developed chitosan nanoparticles prepared using the reverse micelles method, which demonstrated enhanced microbicidal effects against Staphylococcus aureus strains isolated from cows with mastitis, compared to the native polymer. When assessing their effects on the immune system, RAW 264.7 macrophages pretreated with chitosan nanoparticles showed increased production of reactive oxygen species after infection with S. aureus V329, as measured by flow cytometry using the DCFDA probe. Additionally, treated macrophages exhibited upregulation of pro-inflammatory cytokine mRNA, such as IL-1β and IL-6, as evaluated by qPCR, and increased expression of MHCII, CD86, CD11b, and TLR2 markers, as evidenced by flow cytometry. Importantly, the treatment was non- cytotoxic to both murine macrophages and bovine epithelial cells (MAC-T), as determined by the MTT assay. Overall, our findings highlight the potential of chitosan nanoparticles as a novel therapeutic approach to enhance the immune response of the bovine mammary gland and effectively prevent mastitis development following pathogen infections.
Fil: Rodriguez Berdini, Lucia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; Argentina
Fil: Breser, Maria Laura. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; Argentina
Fil: Bohl, Luciana Paola. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; Argentina
Fil: Monaco, Paula. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; Argentina
Fil: Falcone, Ruben Dario. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Porporatto, Carina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; Argentina
14th Latin American and Caribbean Congress of Immunology
Buenos Aires
Argentina
Sociedad Argentina de Inmunología
Latin America and Caribbean Association of Immunology
Asociación de Inmunólogos Clínicos de Argentina
Materia
BOVINE MASTITIS
IMMUNOMODULATION
CHITOSAN
NANOPARTICLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/273043
Acceder
id CONICETDig_cc227d6bc45a5592ce13aa76064da4f8
oai_identifier_str oai:ri.conicet.gov.ar:11336/273043
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chitosan nanoparticles as potential immunomodulatory agents for bovine mastitis preventionRodriguez Berdini, LuciaBreser, Maria LauraBohl, Luciana PaolaMonaco, PaulaFalcone, Ruben DarioPorporatto, CarinaBOVINE MASTITISIMMUNOMODULATIONCHITOSANNANOPARTICLEShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Bovine mastitis is an inflammatory disease of the mammary gland tissue, primarily caused by bacterial infections. This condition is highly relevant in the dairy industry due to its impact on animal welfare and the significant economic losses associated with decreased milk yield and quality. Although antibiotic therapies are still the primary strategy for treating bovine mastitis, the increasing concern about antibiotic-resistant pathogens necessitates reduced antimicrobial use, prompting the search for alternative treatments, especially those derived from natural products. Given that dairy cattle with enhanced and balanced immune responses exhibit a lower incidence of the disease, immunostimulant treatments emerge as a promising alternative. Our research focuses on chitosan, a biocompatible, bioactive, and non-toxic polymer with well-documented antimicrobial and immunostimulant properties. Specifically, we developed chitosan nanoparticles prepared using the reverse micelles method, which demonstrated enhanced microbicidal effects against Staphylococcus aureus strains isolated from cows with mastitis, compared to the native polymer. When assessing their effects on the immune system, RAW 264.7 macrophages pretreated with chitosan nanoparticles showed increased production of reactive oxygen species after infection with S. aureus V329, as measured by flow cytometry using the DCFDA probe. Additionally, treated macrophages exhibited upregulation of pro-inflammatory cytokine mRNA, such as IL-1β and IL-6, as evaluated by qPCR, and increased expression of MHCII, CD86, CD11b, and TLR2 markers, as evidenced by flow cytometry. Importantly, the treatment was non- cytotoxic to both murine macrophages and bovine epithelial cells (MAC-T), as determined by the MTT assay. Overall, our findings highlight the potential of chitosan nanoparticles as a novel therapeutic approach to enhance the immune response of the bovine mammary gland and effectively prevent mastitis development following pathogen infections.Fil: Rodriguez Berdini, Lucia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; ArgentinaFil: Breser, Maria Laura. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; ArgentinaFil: Bohl, Luciana Paola. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; ArgentinaFil: Monaco, Paula. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; ArgentinaFil: Falcone, Ruben Dario. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Porporatto, Carina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; Argentina14th Latin American and Caribbean Congress of ImmunologyBuenos AiresArgentinaSociedad Argentina de InmunologíaLatin America and Caribbean Association of ImmunologyAsociación de Inmunólogos Clínicos de ArgentinaUniversidad Nacional de La Plata2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/273043Chitosan nanoparticles as potential immunomodulatory agents for bovine mastitis prevention; 14th Latin American and Caribbean Congress of Immunology; Buenos Aires; Argentina; 2024; 365-365978-950-34-2435-3CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://alaci.org/alaci-2024-abstracts/info:eu-repo/semantics/altIdentifier/url/https://doi.org/10.35537/10915/173621Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:15:01Zoai:ri.conicet.gov.ar:11336/273043instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:15:02.304CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chitosan nanoparticles as potential immunomodulatory agents for bovine mastitis prevention
title Chitosan nanoparticles as potential immunomodulatory agents for bovine mastitis prevention
spellingShingle Chitosan nanoparticles as potential immunomodulatory agents for bovine mastitis prevention
Rodriguez Berdini, Lucia
BOVINE MASTITIS
IMMUNOMODULATION
CHITOSAN
NANOPARTICLES
title_short Chitosan nanoparticles as potential immunomodulatory agents for bovine mastitis prevention
title_full Chitosan nanoparticles as potential immunomodulatory agents for bovine mastitis prevention
title_fullStr Chitosan nanoparticles as potential immunomodulatory agents for bovine mastitis prevention
title_full_unstemmed Chitosan nanoparticles as potential immunomodulatory agents for bovine mastitis prevention
title_sort Chitosan nanoparticles as potential immunomodulatory agents for bovine mastitis prevention
dc.creator.none.fl_str_mv Rodriguez Berdini, Lucia
Breser, Maria Laura
Bohl, Luciana Paola
Monaco, Paula
Falcone, Ruben Dario
Porporatto, Carina
author Rodriguez Berdini, Lucia
author_facet Rodriguez Berdini, Lucia
Breser, Maria Laura
Bohl, Luciana Paola
Monaco, Paula
Falcone, Ruben Dario
Porporatto, Carina
author_role author
author2 Breser, Maria Laura
Bohl, Luciana Paola
Monaco, Paula
Falcone, Ruben Dario
Porporatto, Carina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BOVINE MASTITIS
IMMUNOMODULATION
CHITOSAN
NANOPARTICLES
topic BOVINE MASTITIS
IMMUNOMODULATION
CHITOSAN
NANOPARTICLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Bovine mastitis is an inflammatory disease of the mammary gland tissue, primarily caused by bacterial infections. This condition is highly relevant in the dairy industry due to its impact on animal welfare and the significant economic losses associated with decreased milk yield and quality. Although antibiotic therapies are still the primary strategy for treating bovine mastitis, the increasing concern about antibiotic-resistant pathogens necessitates reduced antimicrobial use, prompting the search for alternative treatments, especially those derived from natural products. Given that dairy cattle with enhanced and balanced immune responses exhibit a lower incidence of the disease, immunostimulant treatments emerge as a promising alternative. Our research focuses on chitosan, a biocompatible, bioactive, and non-toxic polymer with well-documented antimicrobial and immunostimulant properties. Specifically, we developed chitosan nanoparticles prepared using the reverse micelles method, which demonstrated enhanced microbicidal effects against Staphylococcus aureus strains isolated from cows with mastitis, compared to the native polymer. When assessing their effects on the immune system, RAW 264.7 macrophages pretreated with chitosan nanoparticles showed increased production of reactive oxygen species after infection with S. aureus V329, as measured by flow cytometry using the DCFDA probe. Additionally, treated macrophages exhibited upregulation of pro-inflammatory cytokine mRNA, such as IL-1β and IL-6, as evaluated by qPCR, and increased expression of MHCII, CD86, CD11b, and TLR2 markers, as evidenced by flow cytometry. Importantly, the treatment was non- cytotoxic to both murine macrophages and bovine epithelial cells (MAC-T), as determined by the MTT assay. Overall, our findings highlight the potential of chitosan nanoparticles as a novel therapeutic approach to enhance the immune response of the bovine mammary gland and effectively prevent mastitis development following pathogen infections.
Fil: Rodriguez Berdini, Lucia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; Argentina
Fil: Breser, Maria Laura. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; Argentina
Fil: Bohl, Luciana Paola. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; Argentina
Fil: Monaco, Paula. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; Argentina
Fil: Falcone, Ruben Dario. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Porporatto, Carina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica. - Universidad Nacional de Villa Maria. Instituto Multidisciplinario de Investigacion y Transferencia Agroalimentaria y Biotecnologica.; Argentina
14th Latin American and Caribbean Congress of Immunology
Buenos Aires
Argentina
Sociedad Argentina de Inmunología
Latin America and Caribbean Association of Immunology
Asociación de Inmunólogos Clínicos de Argentina
description Bovine mastitis is an inflammatory disease of the mammary gland tissue, primarily caused by bacterial infections. This condition is highly relevant in the dairy industry due to its impact on animal welfare and the significant economic losses associated with decreased milk yield and quality. Although antibiotic therapies are still the primary strategy for treating bovine mastitis, the increasing concern about antibiotic-resistant pathogens necessitates reduced antimicrobial use, prompting the search for alternative treatments, especially those derived from natural products. Given that dairy cattle with enhanced and balanced immune responses exhibit a lower incidence of the disease, immunostimulant treatments emerge as a promising alternative. Our research focuses on chitosan, a biocompatible, bioactive, and non-toxic polymer with well-documented antimicrobial and immunostimulant properties. Specifically, we developed chitosan nanoparticles prepared using the reverse micelles method, which demonstrated enhanced microbicidal effects against Staphylococcus aureus strains isolated from cows with mastitis, compared to the native polymer. When assessing their effects on the immune system, RAW 264.7 macrophages pretreated with chitosan nanoparticles showed increased production of reactive oxygen species after infection with S. aureus V329, as measured by flow cytometry using the DCFDA probe. Additionally, treated macrophages exhibited upregulation of pro-inflammatory cytokine mRNA, such as IL-1β and IL-6, as evaluated by qPCR, and increased expression of MHCII, CD86, CD11b, and TLR2 markers, as evidenced by flow cytometry. Importantly, the treatment was non- cytotoxic to both murine macrophages and bovine epithelial cells (MAC-T), as determined by the MTT assay. Overall, our findings highlight the potential of chitosan nanoparticles as a novel therapeutic approach to enhance the immune response of the bovine mammary gland and effectively prevent mastitis development following pathogen infections.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/273043
Chitosan nanoparticles as potential immunomodulatory agents for bovine mastitis prevention; 14th Latin American and Caribbean Congress of Immunology; Buenos Aires; Argentina; 2024; 365-365
978-950-34-2435-3
CONICET Digital
CONICET
url http://hdl.handle.net/11336/273043
identifier_str_mv Chitosan nanoparticles as potential immunomodulatory agents for bovine mastitis prevention; 14th Latin American and Caribbean Congress of Immunology; Buenos Aires; Argentina; 2024; 365-365
978-950-34-2435-3
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://alaci.org/alaci-2024-abstracts/
info:eu-repo/semantics/altIdentifier/url/https://doi.org/10.35537/10915/173621
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Universidad Nacional de La Plata
publisher.none.fl_str_mv Universidad Nacional de La Plata
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781578356719616
score 12.982451

Publicaciones similares