Lactic acid bacteria isolated from bovine mammary microbiota: potential allies against bovine Mastitis

Autores
Bouchard, Damien S.; Seridan, Bianca; Saraoui, Taous; Rault, Lucie; Germon, Pierre; Gonzalez Moreno, Candelaria; Nader Macias, Fátima M. E.; Baud, Damien; François, Patrice; Chuat, Victoria; Chain, Florian; Langella, Philippe; Nicoli, Jacques; Le Loir, Yves; Even, Sergine
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine mastitis is a costly disease in dairy cattle worldwide. As of yet, the control of bovine mastitis is mostly based on prevention by thorough hygienic procedures during milking. Additional strategies include vaccination and utilization of antibiotics. Despite these measures, mastitis is not fully under control, thus prompting the need for alternative strategies. The goal of this study was to isolate autochthonous lactic acid bacteria (LAB) from bovine mammary microbiota that exhibit beneficial properties that could be used for mastitis prevention and/or treatment. Sampling of the teat canal led to the isolation of 165 isolates, among which a selection of ten non-redundant LAB strains belonging to the genera Lactobacillus and Lactococcus were further characterized with regard to several properties: surface properties (hydrophobicity, autoaggregation); inhibition potential of three main mastitis pathogens, Staphylococcus aureus, Escherichia coli and Streptococcus uberis; colonization capacities of bovine mammary epithelial cells (bMEC); and immunomodulation properties. Three strains, Lactobacillus brevis 1595 and 1597 and Lactobacillus plantarum 1610, showed high colonization capacities and a medium surface hydrophobicity. These strains are good candidates to compete with pathogens for mammary gland colonization. Moreover, nine strains exhibited anti-inflammatory properties, as illustrated by the lower IL-8 secretion by E. coli-stimulated bMEC in the presence of these LAB. Full genome sequencing of five candidate strains allowed to check for undesirable genetic elements such as antibiotic resistance genes and to identify potential bacterial determinants involved in the beneficial properties. This large screening of beneficial properties while checking for undesirable genetic markers allowed the selection of promising candidate LAB strains from bovine mammary microbiota for the prevention and/or treatment of bovine mastitis.
Fil: Bouchard, Damien S.. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia
Fil: Seridan, Bianca. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia. Universidade Federal do Minas Gerais; Brasil
Fil: Saraoui, Taous. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia
Fil: Rault, Lucie. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia
Fil: Germon, Pierre. Institut National de la Recherche Agronomique; Francia
Fil: Gonzalez Moreno, Candelaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Nader Macias, Fátima M. E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Baud, Damien. Geneva University Hospital; Suiza
Fil: François, Patrice. Geneva University Hospital; Suiza
Fil: Chuat, Victoria. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia
Fil: Chain, Florian. INRA-MICALIS; Francia
Fil: Langella, Philippe. INRA-MICALIS; Francia
Fil: Nicoli, Jacques. Universidade Federal do Minas Gerais; Brasil
Fil: Le Loir, Yves. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia
Fil: Even, Sergine. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia
Materia
Lactic Acid Bacteria
Bovine
Mastitis
Probiotic
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12839
Acceder
id CONICETDig_9e50a8c9d6a5ddcaad126d206e2071c9
oai_identifier_str oai:ri.conicet.gov.ar:11336/12839
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lactic acid bacteria isolated from bovine mammary microbiota: potential allies against bovine MastitisBouchard, Damien S.Seridan, BiancaSaraoui, TaousRault, LucieGermon, PierreGonzalez Moreno, CandelariaNader Macias, Fátima M. E.Baud, DamienFrançois, PatriceChuat, VictoriaChain, FlorianLangella, PhilippeNicoli, JacquesLe Loir, YvesEven, SergineLactic Acid BacteriaBovineMastitisProbiotichttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bovine mastitis is a costly disease in dairy cattle worldwide. As of yet, the control of bovine mastitis is mostly based on prevention by thorough hygienic procedures during milking. Additional strategies include vaccination and utilization of antibiotics. Despite these measures, mastitis is not fully under control, thus prompting the need for alternative strategies. The goal of this study was to isolate autochthonous lactic acid bacteria (LAB) from bovine mammary microbiota that exhibit beneficial properties that could be used for mastitis prevention and/or treatment. Sampling of the teat canal led to the isolation of 165 isolates, among which a selection of ten non-redundant LAB strains belonging to the genera Lactobacillus and Lactococcus were further characterized with regard to several properties: surface properties (hydrophobicity, autoaggregation); inhibition potential of three main mastitis pathogens, Staphylococcus aureus, Escherichia coli and Streptococcus uberis; colonization capacities of bovine mammary epithelial cells (bMEC); and immunomodulation properties. Three strains, Lactobacillus brevis 1595 and 1597 and Lactobacillus plantarum 1610, showed high colonization capacities and a medium surface hydrophobicity. These strains are good candidates to compete with pathogens for mammary gland colonization. Moreover, nine strains exhibited anti-inflammatory properties, as illustrated by the lower IL-8 secretion by E. coli-stimulated bMEC in the presence of these LAB. Full genome sequencing of five candidate strains allowed to check for undesirable genetic elements such as antibiotic resistance genes and to identify potential bacterial determinants involved in the beneficial properties. This large screening of beneficial properties while checking for undesirable genetic markers allowed the selection of promising candidate LAB strains from bovine mammary microbiota for the prevention and/or treatment of bovine mastitis.Fil: Bouchard, Damien S.. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; FranciaFil: Seridan, Bianca. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia. Universidade Federal do Minas Gerais; BrasilFil: Saraoui, Taous. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; FranciaFil: Rault, Lucie. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; FranciaFil: Germon, Pierre. Institut National de la Recherche Agronomique; FranciaFil: Gonzalez Moreno, Candelaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaFil: Nader Macias, Fátima M. E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaFil: Baud, Damien. Geneva University Hospital; SuizaFil: François, Patrice. Geneva University Hospital; SuizaFil: Chuat, Victoria. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; FranciaFil: Chain, Florian. INRA-MICALIS; FranciaFil: Langella, Philippe. INRA-MICALIS; FranciaFil: Nicoli, Jacques. Universidade Federal do Minas Gerais; BrasilFil: Le Loir, Yves. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; FranciaFil: Even, Sergine. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; FranciaPublic Library Of Science2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12839Bouchard, Damien S.; Seridan, Bianca; Saraoui, Taous; Rault, Lucie; Germon, Pierre; et al.; Lactic acid bacteria isolated from bovine mammary microbiota: potential allies against bovine Mastitis; Public Library Of Science; Plos One; 10; 12; 12-2015; 1-18; e01448311932-6203enginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0144831info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0144831info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694705/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:05:21Zoai:ri.conicet.gov.ar:11336/12839instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:05:21.458CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lactic acid bacteria isolated from bovine mammary microbiota: potential allies against bovine Mastitis
title Lactic acid bacteria isolated from bovine mammary microbiota: potential allies against bovine Mastitis
spellingShingle Lactic acid bacteria isolated from bovine mammary microbiota: potential allies against bovine Mastitis
Bouchard, Damien S.
Lactic Acid Bacteria
Bovine
Mastitis
Probiotic
title_short Lactic acid bacteria isolated from bovine mammary microbiota: potential allies against bovine Mastitis
title_full Lactic acid bacteria isolated from bovine mammary microbiota: potential allies against bovine Mastitis
title_fullStr Lactic acid bacteria isolated from bovine mammary microbiota: potential allies against bovine Mastitis
title_full_unstemmed Lactic acid bacteria isolated from bovine mammary microbiota: potential allies against bovine Mastitis
title_sort Lactic acid bacteria isolated from bovine mammary microbiota: potential allies against bovine Mastitis
dc.creator.none.fl_str_mv Bouchard, Damien S.
Seridan, Bianca
Saraoui, Taous
Rault, Lucie
Germon, Pierre
Gonzalez Moreno, Candelaria
Nader Macias, Fátima M. E.
Baud, Damien
François, Patrice
Chuat, Victoria
Chain, Florian
Langella, Philippe
Nicoli, Jacques
Le Loir, Yves
Even, Sergine
author Bouchard, Damien S.
author_facet Bouchard, Damien S.
Seridan, Bianca
Saraoui, Taous
Rault, Lucie
Germon, Pierre
Gonzalez Moreno, Candelaria
Nader Macias, Fátima M. E.
Baud, Damien
François, Patrice
Chuat, Victoria
Chain, Florian
Langella, Philippe
Nicoli, Jacques
Le Loir, Yves
Even, Sergine
author_role author
author2 Seridan, Bianca
Saraoui, Taous
Rault, Lucie
Germon, Pierre
Gonzalez Moreno, Candelaria
Nader Macias, Fátima M. E.
Baud, Damien
François, Patrice
Chuat, Victoria
Chain, Florian
Langella, Philippe
Nicoli, Jacques
Le Loir, Yves
Even, Sergine
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Lactic Acid Bacteria
Bovine
Mastitis
Probiotic
topic Lactic Acid Bacteria
Bovine
Mastitis
Probiotic
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bovine mastitis is a costly disease in dairy cattle worldwide. As of yet, the control of bovine mastitis is mostly based on prevention by thorough hygienic procedures during milking. Additional strategies include vaccination and utilization of antibiotics. Despite these measures, mastitis is not fully under control, thus prompting the need for alternative strategies. The goal of this study was to isolate autochthonous lactic acid bacteria (LAB) from bovine mammary microbiota that exhibit beneficial properties that could be used for mastitis prevention and/or treatment. Sampling of the teat canal led to the isolation of 165 isolates, among which a selection of ten non-redundant LAB strains belonging to the genera Lactobacillus and Lactococcus were further characterized with regard to several properties: surface properties (hydrophobicity, autoaggregation); inhibition potential of three main mastitis pathogens, Staphylococcus aureus, Escherichia coli and Streptococcus uberis; colonization capacities of bovine mammary epithelial cells (bMEC); and immunomodulation properties. Three strains, Lactobacillus brevis 1595 and 1597 and Lactobacillus plantarum 1610, showed high colonization capacities and a medium surface hydrophobicity. These strains are good candidates to compete with pathogens for mammary gland colonization. Moreover, nine strains exhibited anti-inflammatory properties, as illustrated by the lower IL-8 secretion by E. coli-stimulated bMEC in the presence of these LAB. Full genome sequencing of five candidate strains allowed to check for undesirable genetic elements such as antibiotic resistance genes and to identify potential bacterial determinants involved in the beneficial properties. This large screening of beneficial properties while checking for undesirable genetic markers allowed the selection of promising candidate LAB strains from bovine mammary microbiota for the prevention and/or treatment of bovine mastitis.
Fil: Bouchard, Damien S.. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia
Fil: Seridan, Bianca. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia. Universidade Federal do Minas Gerais; Brasil
Fil: Saraoui, Taous. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia
Fil: Rault, Lucie. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia
Fil: Germon, Pierre. Institut National de la Recherche Agronomique; Francia
Fil: Gonzalez Moreno, Candelaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Nader Macias, Fátima M. E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Baud, Damien. Geneva University Hospital; Suiza
Fil: François, Patrice. Geneva University Hospital; Suiza
Fil: Chuat, Victoria. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia
Fil: Chain, Florian. INRA-MICALIS; Francia
Fil: Langella, Philippe. INRA-MICALIS; Francia
Fil: Nicoli, Jacques. Universidade Federal do Minas Gerais; Brasil
Fil: Le Loir, Yves. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia
Fil: Even, Sergine. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia
description Bovine mastitis is a costly disease in dairy cattle worldwide. As of yet, the control of bovine mastitis is mostly based on prevention by thorough hygienic procedures during milking. Additional strategies include vaccination and utilization of antibiotics. Despite these measures, mastitis is not fully under control, thus prompting the need for alternative strategies. The goal of this study was to isolate autochthonous lactic acid bacteria (LAB) from bovine mammary microbiota that exhibit beneficial properties that could be used for mastitis prevention and/or treatment. Sampling of the teat canal led to the isolation of 165 isolates, among which a selection of ten non-redundant LAB strains belonging to the genera Lactobacillus and Lactococcus were further characterized with regard to several properties: surface properties (hydrophobicity, autoaggregation); inhibition potential of three main mastitis pathogens, Staphylococcus aureus, Escherichia coli and Streptococcus uberis; colonization capacities of bovine mammary epithelial cells (bMEC); and immunomodulation properties. Three strains, Lactobacillus brevis 1595 and 1597 and Lactobacillus plantarum 1610, showed high colonization capacities and a medium surface hydrophobicity. These strains are good candidates to compete with pathogens for mammary gland colonization. Moreover, nine strains exhibited anti-inflammatory properties, as illustrated by the lower IL-8 secretion by E. coli-stimulated bMEC in the presence of these LAB. Full genome sequencing of five candidate strains allowed to check for undesirable genetic elements such as antibiotic resistance genes and to identify potential bacterial determinants involved in the beneficial properties. This large screening of beneficial properties while checking for undesirable genetic markers allowed the selection of promising candidate LAB strains from bovine mammary microbiota for the prevention and/or treatment of bovine mastitis.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12839
Bouchard, Damien S.; Seridan, Bianca; Saraoui, Taous; Rault, Lucie; Germon, Pierre; et al.; Lactic acid bacteria isolated from bovine mammary microbiota: potential allies against bovine Mastitis; Public Library Of Science; Plos One; 10; 12; 12-2015; 1-18; e0144831
1932-6203
url http://hdl.handle.net/11336/12839
identifier_str_mv Bouchard, Damien S.; Seridan, Bianca; Saraoui, Taous; Rault, Lucie; Germon, Pierre; et al.; Lactic acid bacteria isolated from bovine mammary microbiota: potential allies against bovine Mastitis; Public Library Of Science; Plos One; 10; 12; 12-2015; 1-18; e0144831
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0144831
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0144831
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694705/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980194820816896
score 12.993085

Publicaciones similares