GdTI, the first thermostable trypsin inhibitor from Geoffroea decorticans seeds : A novel natural drug with potential application in biomedicine

Autores
Cotabarren, Juliana; Broitman, Daiana Judith; Quiroga, Evelina; Obregón, Walter David
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A novel thermostable trypsin inhibitorwas obtained fromGeoffroea decorticans seeds. G. decorticans trypsin inhibitor (GdTI) is a protein with molecular mass of 6743.7 Da, with a potent inhibitory activity (Ki of 2.1 nM) even at high temperatures and extreme pHs (100% after 5 h at 100 °C and 80% after 60 min at pH 2–12) constituting one of the most powerful serine protease inhibitors isolated from a plant source. GdTI displays anticoagulant activity against both extrinsic and intrinsic coagulation pathways, representing the first report of a plant serine protease inhibitor with anticoagulant activity against the extrinsic pathway. Finally, GdTI showed inhibitory activity against α- glucosidase (IC50 of 0.18 μM) evidencing the hypoglycemic effect of this inhibitor. Our results evidence the discovery of a natural molecule with unique features: i) GdTI is one of the most potent serine protease inhibitors founded to date, ii)with themost powerful thermostability reported in literature, iii)with anticoagulant effect against both coagulation pathways and hypoglycemic activity. This report suggest that GdTI could be exploited as a natural and hyperstable antidiabetic drug, in behalf of its antithrombotic and hypoglycemic activities, encouraging future studies with high impact on biomedical research and potential pharmaceutical applications.
Centro de Investigación de Proteínas Vegetales
Materia
Biología
Geoffroea decorticans
Trypsin inhibitor
Serine protease inhibitor
Anticoagulant activity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/164072

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oai_identifier_str oai:sedici.unlp.edu.ar:10915/164072
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling GdTI, the first thermostable trypsin inhibitor from Geoffroea decorticans seeds : A novel natural drug with potential application in biomedicineCotabarren, JulianaBroitman, Daiana JudithQuiroga, EvelinaObregón, Walter DavidBiologíaGeoffroea decorticansTrypsin inhibitorSerine protease inhibitorAnticoagulant activityA novel thermostable trypsin inhibitorwas obtained fromGeoffroea decorticans seeds. G. decorticans trypsin inhibitor (GdTI) is a protein with molecular mass of 6743.7 Da, with a potent inhibitory activity (Ki of 2.1 nM) even at high temperatures and extreme pHs (100% after 5 h at 100 °C and 80% after 60 min at pH 2–12) constituting one of the most powerful serine protease inhibitors isolated from a plant source. GdTI displays anticoagulant activity against both extrinsic and intrinsic coagulation pathways, representing the first report of a plant serine protease inhibitor with anticoagulant activity against the extrinsic pathway. Finally, GdTI showed inhibitory activity against α- glucosidase (IC50 of 0.18 μM) evidencing the hypoglycemic effect of this inhibitor. Our results evidence the discovery of a natural molecule with unique features: i) GdTI is one of the most potent serine protease inhibitors founded to date, ii)with themost powerful thermostability reported in literature, iii)with anticoagulant effect against both coagulation pathways and hypoglycemic activity. This report suggest that GdTI could be exploited as a natural and hyperstable antidiabetic drug, in behalf of its antithrombotic and hypoglycemic activities, encouraging future studies with high impact on biomedical research and potential pharmaceutical applications.Centro de Investigación de Proteínas Vegetales2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf869-879http://sedici.unlp.edu.ar/handle/10915/164072enginfo:eu-repo/semantics/altIdentifier/issn/0141-8130info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2020.01.214info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:15:06Zoai:sedici.unlp.edu.ar:10915/164072Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:15:06.639SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv GdTI, the first thermostable trypsin inhibitor from Geoffroea decorticans seeds : A novel natural drug with potential application in biomedicine
title GdTI, the first thermostable trypsin inhibitor from Geoffroea decorticans seeds : A novel natural drug with potential application in biomedicine
spellingShingle GdTI, the first thermostable trypsin inhibitor from Geoffroea decorticans seeds : A novel natural drug with potential application in biomedicine
Cotabarren, Juliana
Biología
Geoffroea decorticans
Trypsin inhibitor
Serine protease inhibitor
Anticoagulant activity
title_short GdTI, the first thermostable trypsin inhibitor from Geoffroea decorticans seeds : A novel natural drug with potential application in biomedicine
title_full GdTI, the first thermostable trypsin inhibitor from Geoffroea decorticans seeds : A novel natural drug with potential application in biomedicine
title_fullStr GdTI, the first thermostable trypsin inhibitor from Geoffroea decorticans seeds : A novel natural drug with potential application in biomedicine
title_full_unstemmed GdTI, the first thermostable trypsin inhibitor from Geoffroea decorticans seeds : A novel natural drug with potential application in biomedicine
title_sort GdTI, the first thermostable trypsin inhibitor from Geoffroea decorticans seeds : A novel natural drug with potential application in biomedicine
dc.creator.none.fl_str_mv Cotabarren, Juliana
Broitman, Daiana Judith
Quiroga, Evelina
Obregón, Walter David
author Cotabarren, Juliana
author_facet Cotabarren, Juliana
Broitman, Daiana Judith
Quiroga, Evelina
Obregón, Walter David
author_role author
author2 Broitman, Daiana Judith
Quiroga, Evelina
Obregón, Walter David
author2_role author
author
author
dc.subject.none.fl_str_mv Biología
Geoffroea decorticans
Trypsin inhibitor
Serine protease inhibitor
Anticoagulant activity
topic Biología
Geoffroea decorticans
Trypsin inhibitor
Serine protease inhibitor
Anticoagulant activity
dc.description.none.fl_txt_mv A novel thermostable trypsin inhibitorwas obtained fromGeoffroea decorticans seeds. G. decorticans trypsin inhibitor (GdTI) is a protein with molecular mass of 6743.7 Da, with a potent inhibitory activity (Ki of 2.1 nM) even at high temperatures and extreme pHs (100% after 5 h at 100 °C and 80% after 60 min at pH 2–12) constituting one of the most powerful serine protease inhibitors isolated from a plant source. GdTI displays anticoagulant activity against both extrinsic and intrinsic coagulation pathways, representing the first report of a plant serine protease inhibitor with anticoagulant activity against the extrinsic pathway. Finally, GdTI showed inhibitory activity against α- glucosidase (IC50 of 0.18 μM) evidencing the hypoglycemic effect of this inhibitor. Our results evidence the discovery of a natural molecule with unique features: i) GdTI is one of the most potent serine protease inhibitors founded to date, ii)with themost powerful thermostability reported in literature, iii)with anticoagulant effect against both coagulation pathways and hypoglycemic activity. This report suggest that GdTI could be exploited as a natural and hyperstable antidiabetic drug, in behalf of its antithrombotic and hypoglycemic activities, encouraging future studies with high impact on biomedical research and potential pharmaceutical applications.
Centro de Investigación de Proteínas Vegetales
description A novel thermostable trypsin inhibitorwas obtained fromGeoffroea decorticans seeds. G. decorticans trypsin inhibitor (GdTI) is a protein with molecular mass of 6743.7 Da, with a potent inhibitory activity (Ki of 2.1 nM) even at high temperatures and extreme pHs (100% after 5 h at 100 °C and 80% after 60 min at pH 2–12) constituting one of the most powerful serine protease inhibitors isolated from a plant source. GdTI displays anticoagulant activity against both extrinsic and intrinsic coagulation pathways, representing the first report of a plant serine protease inhibitor with anticoagulant activity against the extrinsic pathway. Finally, GdTI showed inhibitory activity against α- glucosidase (IC50 of 0.18 μM) evidencing the hypoglycemic effect of this inhibitor. Our results evidence the discovery of a natural molecule with unique features: i) GdTI is one of the most potent serine protease inhibitors founded to date, ii)with themost powerful thermostability reported in literature, iii)with anticoagulant effect against both coagulation pathways and hypoglycemic activity. This report suggest that GdTI could be exploited as a natural and hyperstable antidiabetic drug, in behalf of its antithrombotic and hypoglycemic activities, encouraging future studies with high impact on biomedical research and potential pharmaceutical applications.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/164072
url http://sedici.unlp.edu.ar/handle/10915/164072
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0141-8130
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2020.01.214
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
869-879
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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