Beta-cyclodextrin and permeability to water in the bladder of Bufo arenarum

Autores
Orce, Gabriel Guillermo F.; Castillo G.; Chanampa Y.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We measured the effect of β-cyclodextrin (BCD, a cholesterol scavenger) on water flow across the isolated toad bladder exposed to an osmotic gradient (Jw) by a gravimetric technique. BCD, when present in the solution bathing the apical side of the bladder, inhibited the increase in Jw caused by nystatin, a polyene antibiotic that acts by directly binding apical membrane cholesterol. When present in the basolateral bath, BCD inhibited the increase in Jw caused by basolateral exposure to oxytocin (which binds membrane receptors and stimulates the synthesis of cAMP), but did not alter the response to theophylline (which inhibits hydrolysis of cAMP by cyclic nucleotide phosphodiesterase). The present data are consistent with the notion that agents that increase Jw by interacting with membrane receptors, which appear to be clustered in cholesterol-rich domains of the basolateral membrane, are altered by cholesterol depletion, whereas agents that do not interact with receptors or other basolateral membrane components are not affected by this treatment. In either case, cholesterol depletion of the apical membrane does not affect the increase in Jw brought about by an increase in intracellular cAMP concentration.
Fil: Orce, Gabriel Guillermo F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
Fil: Castillo G.. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Chanampa Y.. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
Materia
Beta-Cyclodextrin
Water Flow
Epithelial Permeability
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/77038
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Beta-cyclodextrin and permeability to water in the bladder of Bufo arenarumOrce, Gabriel Guillermo F.Castillo G.Chanampa Y.Beta-CyclodextrinWater FlowEpithelial Permeabilityhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3We measured the effect of β-cyclodextrin (BCD, a cholesterol scavenger) on water flow across the isolated toad bladder exposed to an osmotic gradient (Jw) by a gravimetric technique. BCD, when present in the solution bathing the apical side of the bladder, inhibited the increase in Jw caused by nystatin, a polyene antibiotic that acts by directly binding apical membrane cholesterol. When present in the basolateral bath, BCD inhibited the increase in Jw caused by basolateral exposure to oxytocin (which binds membrane receptors and stimulates the synthesis of cAMP), but did not alter the response to theophylline (which inhibits hydrolysis of cAMP by cyclic nucleotide phosphodiesterase). The present data are consistent with the notion that agents that increase Jw by interacting with membrane receptors, which appear to be clustered in cholesterol-rich domains of the basolateral membrane, are altered by cholesterol depletion, whereas agents that do not interact with receptors or other basolateral membrane components are not affected by this treatment. In either case, cholesterol depletion of the apical membrane does not affect the increase in Jw brought about by an increase in intracellular cAMP concentration.Fil: Orce, Gabriel Guillermo F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; ArgentinaFil: Castillo G.. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Chanampa Y.. Universidad Nacional de Tucumán. Facultad de Medicina; ArgentinaNational Research Council Canada-NRC Research Press2011-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/77038Orce, Gabriel Guillermo F.; Castillo G.; Chanampa Y.; Beta-cyclodextrin and permeability to water in the bladder of Bufo arenarum; National Research Council Canada-NRC Research Press; Canadian Journal Of Physiology And Pharmacology; 89; 5; 5-2011; 311-3150008-4212CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1139/Y11-026info:eu-repo/semantics/altIdentifier/url/https://www.nrcresearchpress.com/doi/10.1139/y11-026info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:07:26Zoai:ri.conicet.gov.ar:11336/77038instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:07:26.493CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Beta-cyclodextrin and permeability to water in the bladder of Bufo arenarum
title Beta-cyclodextrin and permeability to water in the bladder of Bufo arenarum
spellingShingle Beta-cyclodextrin and permeability to water in the bladder of Bufo arenarum
Orce, Gabriel Guillermo F.
Beta-Cyclodextrin
Water Flow
Epithelial Permeability
title_short Beta-cyclodextrin and permeability to water in the bladder of Bufo arenarum
title_full Beta-cyclodextrin and permeability to water in the bladder of Bufo arenarum
title_fullStr Beta-cyclodextrin and permeability to water in the bladder of Bufo arenarum
title_full_unstemmed Beta-cyclodextrin and permeability to water in the bladder of Bufo arenarum
title_sort Beta-cyclodextrin and permeability to water in the bladder of Bufo arenarum
dc.creator.none.fl_str_mv Orce, Gabriel Guillermo F.
Castillo G.
Chanampa Y.
author Orce, Gabriel Guillermo F.
author_facet Orce, Gabriel Guillermo F.
Castillo G.
Chanampa Y.
author_role author
author2 Castillo G.
Chanampa Y.
author2_role author
author
dc.subject.none.fl_str_mv Beta-Cyclodextrin
Water Flow
Epithelial Permeability
topic Beta-Cyclodextrin
Water Flow
Epithelial Permeability
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We measured the effect of β-cyclodextrin (BCD, a cholesterol scavenger) on water flow across the isolated toad bladder exposed to an osmotic gradient (Jw) by a gravimetric technique. BCD, when present in the solution bathing the apical side of the bladder, inhibited the increase in Jw caused by nystatin, a polyene antibiotic that acts by directly binding apical membrane cholesterol. When present in the basolateral bath, BCD inhibited the increase in Jw caused by basolateral exposure to oxytocin (which binds membrane receptors and stimulates the synthesis of cAMP), but did not alter the response to theophylline (which inhibits hydrolysis of cAMP by cyclic nucleotide phosphodiesterase). The present data are consistent with the notion that agents that increase Jw by interacting with membrane receptors, which appear to be clustered in cholesterol-rich domains of the basolateral membrane, are altered by cholesterol depletion, whereas agents that do not interact with receptors or other basolateral membrane components are not affected by this treatment. In either case, cholesterol depletion of the apical membrane does not affect the increase in Jw brought about by an increase in intracellular cAMP concentration.
Fil: Orce, Gabriel Guillermo F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
Fil: Castillo G.. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Chanampa Y.. Universidad Nacional de Tucumán. Facultad de Medicina; Argentina
description We measured the effect of β-cyclodextrin (BCD, a cholesterol scavenger) on water flow across the isolated toad bladder exposed to an osmotic gradient (Jw) by a gravimetric technique. BCD, when present in the solution bathing the apical side of the bladder, inhibited the increase in Jw caused by nystatin, a polyene antibiotic that acts by directly binding apical membrane cholesterol. When present in the basolateral bath, BCD inhibited the increase in Jw caused by basolateral exposure to oxytocin (which binds membrane receptors and stimulates the synthesis of cAMP), but did not alter the response to theophylline (which inhibits hydrolysis of cAMP by cyclic nucleotide phosphodiesterase). The present data are consistent with the notion that agents that increase Jw by interacting with membrane receptors, which appear to be clustered in cholesterol-rich domains of the basolateral membrane, are altered by cholesterol depletion, whereas agents that do not interact with receptors or other basolateral membrane components are not affected by this treatment. In either case, cholesterol depletion of the apical membrane does not affect the increase in Jw brought about by an increase in intracellular cAMP concentration.
publishDate 2011
dc.date.none.fl_str_mv 2011-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/77038
Orce, Gabriel Guillermo F.; Castillo G.; Chanampa Y.; Beta-cyclodextrin and permeability to water in the bladder of Bufo arenarum; National Research Council Canada-NRC Research Press; Canadian Journal Of Physiology And Pharmacology; 89; 5; 5-2011; 311-315
0008-4212
CONICET Digital
CONICET
url http://hdl.handle.net/11336/77038
identifier_str_mv Orce, Gabriel Guillermo F.; Castillo G.; Chanampa Y.; Beta-cyclodextrin and permeability to water in the bladder of Bufo arenarum; National Research Council Canada-NRC Research Press; Canadian Journal Of Physiology And Pharmacology; 89; 5; 5-2011; 311-315
0008-4212
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1139/Y11-026
info:eu-repo/semantics/altIdentifier/url/https://www.nrcresearchpress.com/doi/10.1139/y11-026
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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