Mechanisms involved in the improvement of lipotoxicity and impaired lipid metabolism by dietary α-linolenic acid rich salvia hispanica L (Salba) seed in the heart of dyslipemic ins...

Autores
Creus, Agustina; Ferreira Ruiz, María José; Oliva, Maria Eugenia; Lombardo, Yolanda B.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study explores the mechanisms underlying the altered lipid metabolism in the heart of dyslipemic insulin-resistant (IR) rats fed a sucrose-rich diet (SRD) and investigates if chia seeds (rich in α-linolenic acid 18:3, n-3 ALA) improve/reverse cardiac lipotoxicity. Wistar rats received an SRD-diet for three months. Half of the animals continued with the SRD up to month 6. The other half was fed an SRD in which the fat source, corn oil (CO), was replaced by chia seeds from month 3 to 6 (SRD+chia). A reference group consumed a control diet (CD) all the time. Triglyceride, long-chain acyl CoA (LC ACoA) and diacylglycerol (DAG) contents, pyruvate dehydrogenase complex (PDHc) and muscle-type carnitine palmitoyltransferase 1 (M-CPT1) activities and protein mass levels of M-CPT1, membrane fatty acid transporter (FAT/CD36), peroxisome proliferator activated receptor α (PPARα) and uncoupling protein 2 (UCP2) were analyzed. Results show that: (a) the hearts of SRD-fed rats display lipotoxicity suggesting impaired myocardial lipid utilization; (b) Compared with the SRD group, dietary chia normalizes blood pressure; reverses/improves heart lipotoxicity, glucose oxidation, the increased protein mass level of FAT/CD36, and the impaired insulin stimulated FAT/CD36 translocation to the plasma membrane. The enhanced M-CPT1 activity is markedly reduced without similar changes in protein mass. PPARα slightly decreases, while the UCP2 protein level remains unchanged in all groups. Normalization of dyslipidemia and IR by chia reduces plasma fatty acids (FAs) availability, suggesting that a different milieu prevents the robust translocation of FAT/CD36. This could reduce the influx of FAs, decreasing the elevated M-CPT1 activity and lipid storage and improving glucose oxidation in cardiac muscles of SRD-fed rats.
Fil: Creus, Agustina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Ferreira Ruiz, María José. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Oliva, Maria Eugenia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Lombardo, Yolanda B.. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Química Biológica; Argentina
Materia
CARDIAC MUSCLE
DYSLIPIDEMIA
HIGH-SUCROSE DIET
INSULIN RESISTANCE
LIPOTOXICITY
Α-LINOLENIC ACID (ALA)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181095
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/181095
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mechanisms involved in the improvement of lipotoxicity and impaired lipid metabolism by dietary α-linolenic acid rich salvia hispanica L (Salba) seed in the heart of dyslipemic insulin-resistant ratsCreus, AgustinaFerreira Ruiz, María JoséOliva, Maria EugeniaLombardo, Yolanda B.CARDIAC MUSCLEDYSLIPIDEMIAHIGH-SUCROSE DIETINSULIN RESISTANCELIPOTOXICITYΑ-LINOLENIC ACID (ALA)https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This study explores the mechanisms underlying the altered lipid metabolism in the heart of dyslipemic insulin-resistant (IR) rats fed a sucrose-rich diet (SRD) and investigates if chia seeds (rich in α-linolenic acid 18:3, n-3 ALA) improve/reverse cardiac lipotoxicity. Wistar rats received an SRD-diet for three months. Half of the animals continued with the SRD up to month 6. The other half was fed an SRD in which the fat source, corn oil (CO), was replaced by chia seeds from month 3 to 6 (SRD+chia). A reference group consumed a control diet (CD) all the time. Triglyceride, long-chain acyl CoA (LC ACoA) and diacylglycerol (DAG) contents, pyruvate dehydrogenase complex (PDHc) and muscle-type carnitine palmitoyltransferase 1 (M-CPT1) activities and protein mass levels of M-CPT1, membrane fatty acid transporter (FAT/CD36), peroxisome proliferator activated receptor α (PPARα) and uncoupling protein 2 (UCP2) were analyzed. Results show that: (a) the hearts of SRD-fed rats display lipotoxicity suggesting impaired myocardial lipid utilization; (b) Compared with the SRD group, dietary chia normalizes blood pressure; reverses/improves heart lipotoxicity, glucose oxidation, the increased protein mass level of FAT/CD36, and the impaired insulin stimulated FAT/CD36 translocation to the plasma membrane. The enhanced M-CPT1 activity is markedly reduced without similar changes in protein mass. PPARα slightly decreases, while the UCP2 protein level remains unchanged in all groups. Normalization of dyslipidemia and IR by chia reduces plasma fatty acids (FAs) availability, suggesting that a different milieu prevents the robust translocation of FAT/CD36. This could reduce the influx of FAs, decreasing the elevated M-CPT1 activity and lipid storage and improving glucose oxidation in cardiac muscles of SRD-fed rats.Fil: Creus, Agustina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Ferreira Ruiz, María José. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Oliva, Maria Eugenia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Lombardo, Yolanda B.. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Química Biológica; ArgentinaMultidisciplinary Digital Publishing Institute2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181095Creus, Agustina; Ferreira Ruiz, María José; Oliva, Maria Eugenia; Lombardo, Yolanda B.; Mechanisms involved in the improvement of lipotoxicity and impaired lipid metabolism by dietary α-linolenic acid rich salvia hispanica L (Salba) seed in the heart of dyslipemic insulin-resistant rats; Multidisciplinary Digital Publishing Institute; Journal of Clinical Medicine; 5; 2; 1-2016; 1-162077-0383CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/jcm5020018info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2077-0383/5/2/18info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:39:18Zoai:ri.conicet.gov.ar:11336/181095instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:39:18.314CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mechanisms involved in the improvement of lipotoxicity and impaired lipid metabolism by dietary α-linolenic acid rich salvia hispanica L (Salba) seed in the heart of dyslipemic insulin-resistant rats
title Mechanisms involved in the improvement of lipotoxicity and impaired lipid metabolism by dietary α-linolenic acid rich salvia hispanica L (Salba) seed in the heart of dyslipemic insulin-resistant rats
spellingShingle Mechanisms involved in the improvement of lipotoxicity and impaired lipid metabolism by dietary α-linolenic acid rich salvia hispanica L (Salba) seed in the heart of dyslipemic insulin-resistant rats
Creus, Agustina
CARDIAC MUSCLE
DYSLIPIDEMIA
HIGH-SUCROSE DIET
INSULIN RESISTANCE
LIPOTOXICITY
Α-LINOLENIC ACID (ALA)
title_short Mechanisms involved in the improvement of lipotoxicity and impaired lipid metabolism by dietary α-linolenic acid rich salvia hispanica L (Salba) seed in the heart of dyslipemic insulin-resistant rats
title_full Mechanisms involved in the improvement of lipotoxicity and impaired lipid metabolism by dietary α-linolenic acid rich salvia hispanica L (Salba) seed in the heart of dyslipemic insulin-resistant rats
title_fullStr Mechanisms involved in the improvement of lipotoxicity and impaired lipid metabolism by dietary α-linolenic acid rich salvia hispanica L (Salba) seed in the heart of dyslipemic insulin-resistant rats
title_full_unstemmed Mechanisms involved in the improvement of lipotoxicity and impaired lipid metabolism by dietary α-linolenic acid rich salvia hispanica L (Salba) seed in the heart of dyslipemic insulin-resistant rats
title_sort Mechanisms involved in the improvement of lipotoxicity and impaired lipid metabolism by dietary α-linolenic acid rich salvia hispanica L (Salba) seed in the heart of dyslipemic insulin-resistant rats
dc.creator.none.fl_str_mv Creus, Agustina
Ferreira Ruiz, María José
Oliva, Maria Eugenia
Lombardo, Yolanda B.
author Creus, Agustina
author_facet Creus, Agustina
Ferreira Ruiz, María José
Oliva, Maria Eugenia
Lombardo, Yolanda B.
author_role author
author2 Ferreira Ruiz, María José
Oliva, Maria Eugenia
Lombardo, Yolanda B.
author2_role author
author
author
dc.subject.none.fl_str_mv CARDIAC MUSCLE
DYSLIPIDEMIA
HIGH-SUCROSE DIET
INSULIN RESISTANCE
LIPOTOXICITY
Α-LINOLENIC ACID (ALA)
topic CARDIAC MUSCLE
DYSLIPIDEMIA
HIGH-SUCROSE DIET
INSULIN RESISTANCE
LIPOTOXICITY
Α-LINOLENIC ACID (ALA)
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This study explores the mechanisms underlying the altered lipid metabolism in the heart of dyslipemic insulin-resistant (IR) rats fed a sucrose-rich diet (SRD) and investigates if chia seeds (rich in α-linolenic acid 18:3, n-3 ALA) improve/reverse cardiac lipotoxicity. Wistar rats received an SRD-diet for three months. Half of the animals continued with the SRD up to month 6. The other half was fed an SRD in which the fat source, corn oil (CO), was replaced by chia seeds from month 3 to 6 (SRD+chia). A reference group consumed a control diet (CD) all the time. Triglyceride, long-chain acyl CoA (LC ACoA) and diacylglycerol (DAG) contents, pyruvate dehydrogenase complex (PDHc) and muscle-type carnitine palmitoyltransferase 1 (M-CPT1) activities and protein mass levels of M-CPT1, membrane fatty acid transporter (FAT/CD36), peroxisome proliferator activated receptor α (PPARα) and uncoupling protein 2 (UCP2) were analyzed. Results show that: (a) the hearts of SRD-fed rats display lipotoxicity suggesting impaired myocardial lipid utilization; (b) Compared with the SRD group, dietary chia normalizes blood pressure; reverses/improves heart lipotoxicity, glucose oxidation, the increased protein mass level of FAT/CD36, and the impaired insulin stimulated FAT/CD36 translocation to the plasma membrane. The enhanced M-CPT1 activity is markedly reduced without similar changes in protein mass. PPARα slightly decreases, while the UCP2 protein level remains unchanged in all groups. Normalization of dyslipidemia and IR by chia reduces plasma fatty acids (FAs) availability, suggesting that a different milieu prevents the robust translocation of FAT/CD36. This could reduce the influx of FAs, decreasing the elevated M-CPT1 activity and lipid storage and improving glucose oxidation in cardiac muscles of SRD-fed rats.
Fil: Creus, Agustina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Ferreira Ruiz, María José. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Oliva, Maria Eugenia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Lombardo, Yolanda B.. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Química Biológica; Argentina
description This study explores the mechanisms underlying the altered lipid metabolism in the heart of dyslipemic insulin-resistant (IR) rats fed a sucrose-rich diet (SRD) and investigates if chia seeds (rich in α-linolenic acid 18:3, n-3 ALA) improve/reverse cardiac lipotoxicity. Wistar rats received an SRD-diet for three months. Half of the animals continued with the SRD up to month 6. The other half was fed an SRD in which the fat source, corn oil (CO), was replaced by chia seeds from month 3 to 6 (SRD+chia). A reference group consumed a control diet (CD) all the time. Triglyceride, long-chain acyl CoA (LC ACoA) and diacylglycerol (DAG) contents, pyruvate dehydrogenase complex (PDHc) and muscle-type carnitine palmitoyltransferase 1 (M-CPT1) activities and protein mass levels of M-CPT1, membrane fatty acid transporter (FAT/CD36), peroxisome proliferator activated receptor α (PPARα) and uncoupling protein 2 (UCP2) were analyzed. Results show that: (a) the hearts of SRD-fed rats display lipotoxicity suggesting impaired myocardial lipid utilization; (b) Compared with the SRD group, dietary chia normalizes blood pressure; reverses/improves heart lipotoxicity, glucose oxidation, the increased protein mass level of FAT/CD36, and the impaired insulin stimulated FAT/CD36 translocation to the plasma membrane. The enhanced M-CPT1 activity is markedly reduced without similar changes in protein mass. PPARα slightly decreases, while the UCP2 protein level remains unchanged in all groups. Normalization of dyslipidemia and IR by chia reduces plasma fatty acids (FAs) availability, suggesting that a different milieu prevents the robust translocation of FAT/CD36. This could reduce the influx of FAs, decreasing the elevated M-CPT1 activity and lipid storage and improving glucose oxidation in cardiac muscles of SRD-fed rats.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181095
Creus, Agustina; Ferreira Ruiz, María José; Oliva, Maria Eugenia; Lombardo, Yolanda B.; Mechanisms involved in the improvement of lipotoxicity and impaired lipid metabolism by dietary α-linolenic acid rich salvia hispanica L (Salba) seed in the heart of dyslipemic insulin-resistant rats; Multidisciplinary Digital Publishing Institute; Journal of Clinical Medicine; 5; 2; 1-2016; 1-16
2077-0383
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181095
identifier_str_mv Creus, Agustina; Ferreira Ruiz, María José; Oliva, Maria Eugenia; Lombardo, Yolanda B.; Mechanisms involved in the improvement of lipotoxicity and impaired lipid metabolism by dietary α-linolenic acid rich salvia hispanica L (Salba) seed in the heart of dyslipemic insulin-resistant rats; Multidisciplinary Digital Publishing Institute; Journal of Clinical Medicine; 5; 2; 1-2016; 1-16
2077-0383
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/jcm5020018
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2077-0383/5/2/18
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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