Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects

Autores
Volfson, Victoria; Fibach Paldi, Sharon; Paulucci, Natalia Soledad; Dardanelli, Marta Susana; Matan, Ofra; Burdman, Saul; Okon, Yaacov
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Azospirillum genus comprises free-living, plant growth-promoting, nitrogen-fixing bacteria found in the rhizosphere of plant roots. Azospirilla are able to promote plant growth mainly through improvement of root development. Bacterial surface components, such as extracellular polysaccharides and proteins, are involved in root colonization. Phase variation – or phenotypic variation – is one of the mechanisms by which microorganisms adapt to environmental changes. This phenomenon is characterized by the presence of a sub-population of the bacteria presenting a different phenotype relative to the major population. In this study we characterized phenotypic variation of Azospirillum brasilense Sp7. When plated on solid media, some A. brasilense colonies were shown to possess a much more mucoid morphology, producing 7.5–8 times more exopolysaccharide with different monosaccharide composition than the parental strain Sp7. The rate of appearance of this kind of variant colonies was 1 in 5000, in agreement with the accepted rate for the phase/phenotypic variation phenomenon. The variants were significantly more resistant to heat and UV-exposure than the parental strain and displayed genomic changes as seen by the different band patterns following ERIC-PCR, BOX-PCR and RAPD analyses. In plant inoculation experiments under greenhouse conditions, with maize, wheat, soybean and peanuts, the EPS overproducing variants performed as similar as the parental strain. Therefore, EPS overproduction did not confer an apparent advantage to A. brasilense in terms of induction of plant growth promotion.
Fil: Volfson, Victoria. The Hebrew University Of Jerusalem; Israel
Fil: Fibach Paldi, Sharon. The Hebrew University Of Jerusalem; Israel
Fil: Paulucci, Natalia Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Matan, Ofra. The Hebrew University Of Jerusalem; Israel
Fil: Burdman, Saul. The Hebrew University Of Jerusalem; Israel
Fil: Okon, Yaacov. The Hebrew University Of Jerusalem; Israel
Materia
Azospirillum Brasilense
Phase Variation
Plant Growth Promotion
Genomic Changes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24138

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network_name_str CONICET Digital (CONICET)
spelling Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effectsVolfson, VictoriaFibach Paldi, SharonPaulucci, Natalia SoledadDardanelli, Marta SusanaMatan, OfraBurdman, SaulOkon, YaacovAzospirillum BrasilensePhase VariationPlant Growth PromotionGenomic Changeshttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The Azospirillum genus comprises free-living, plant growth-promoting, nitrogen-fixing bacteria found in the rhizosphere of plant roots. Azospirilla are able to promote plant growth mainly through improvement of root development. Bacterial surface components, such as extracellular polysaccharides and proteins, are involved in root colonization. Phase variation – or phenotypic variation – is one of the mechanisms by which microorganisms adapt to environmental changes. This phenomenon is characterized by the presence of a sub-population of the bacteria presenting a different phenotype relative to the major population. In this study we characterized phenotypic variation of Azospirillum brasilense Sp7. When plated on solid media, some A. brasilense colonies were shown to possess a much more mucoid morphology, producing 7.5–8 times more exopolysaccharide with different monosaccharide composition than the parental strain Sp7. The rate of appearance of this kind of variant colonies was 1 in 5000, in agreement with the accepted rate for the phase/phenotypic variation phenomenon. The variants were significantly more resistant to heat and UV-exposure than the parental strain and displayed genomic changes as seen by the different band patterns following ERIC-PCR, BOX-PCR and RAPD analyses. In plant inoculation experiments under greenhouse conditions, with maize, wheat, soybean and peanuts, the EPS overproducing variants performed as similar as the parental strain. Therefore, EPS overproduction did not confer an apparent advantage to A. brasilense in terms of induction of plant growth promotion.Fil: Volfson, Victoria. The Hebrew University Of Jerusalem; IsraelFil: Fibach Paldi, Sharon. The Hebrew University Of Jerusalem; IsraelFil: Paulucci, Natalia Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Matan, Ofra. The Hebrew University Of Jerusalem; IsraelFil: Burdman, Saul. The Hebrew University Of Jerusalem; IsraelFil: Okon, Yaacov. The Hebrew University Of Jerusalem; IsraelElsevier2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24138Volfson, Victoria; Fibach Paldi, Sharon; Paulucci, Natalia Soledad; Dardanelli, Marta Susana; Matan, Ofra; et al.; Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects; Elsevier; Soil Biology And Biochemistry; 67; 9-2013; 255-2620038-0717CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.soilbio.2013.09.008info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S003807171300309Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:46Zoai:ri.conicet.gov.ar:11336/24138instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:46.573CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects
title Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects
spellingShingle Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects
Volfson, Victoria
Azospirillum Brasilense
Phase Variation
Plant Growth Promotion
Genomic Changes
title_short Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects
title_full Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects
title_fullStr Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects
title_full_unstemmed Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects
title_sort Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects
dc.creator.none.fl_str_mv Volfson, Victoria
Fibach Paldi, Sharon
Paulucci, Natalia Soledad
Dardanelli, Marta Susana
Matan, Ofra
Burdman, Saul
Okon, Yaacov
author Volfson, Victoria
author_facet Volfson, Victoria
Fibach Paldi, Sharon
Paulucci, Natalia Soledad
Dardanelli, Marta Susana
Matan, Ofra
Burdman, Saul
Okon, Yaacov
author_role author
author2 Fibach Paldi, Sharon
Paulucci, Natalia Soledad
Dardanelli, Marta Susana
Matan, Ofra
Burdman, Saul
Okon, Yaacov
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Azospirillum Brasilense
Phase Variation
Plant Growth Promotion
Genomic Changes
topic Azospirillum Brasilense
Phase Variation
Plant Growth Promotion
Genomic Changes
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The Azospirillum genus comprises free-living, plant growth-promoting, nitrogen-fixing bacteria found in the rhizosphere of plant roots. Azospirilla are able to promote plant growth mainly through improvement of root development. Bacterial surface components, such as extracellular polysaccharides and proteins, are involved in root colonization. Phase variation – or phenotypic variation – is one of the mechanisms by which microorganisms adapt to environmental changes. This phenomenon is characterized by the presence of a sub-population of the bacteria presenting a different phenotype relative to the major population. In this study we characterized phenotypic variation of Azospirillum brasilense Sp7. When plated on solid media, some A. brasilense colonies were shown to possess a much more mucoid morphology, producing 7.5–8 times more exopolysaccharide with different monosaccharide composition than the parental strain Sp7. The rate of appearance of this kind of variant colonies was 1 in 5000, in agreement with the accepted rate for the phase/phenotypic variation phenomenon. The variants were significantly more resistant to heat and UV-exposure than the parental strain and displayed genomic changes as seen by the different band patterns following ERIC-PCR, BOX-PCR and RAPD analyses. In plant inoculation experiments under greenhouse conditions, with maize, wheat, soybean and peanuts, the EPS overproducing variants performed as similar as the parental strain. Therefore, EPS overproduction did not confer an apparent advantage to A. brasilense in terms of induction of plant growth promotion.
Fil: Volfson, Victoria. The Hebrew University Of Jerusalem; Israel
Fil: Fibach Paldi, Sharon. The Hebrew University Of Jerusalem; Israel
Fil: Paulucci, Natalia Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Matan, Ofra. The Hebrew University Of Jerusalem; Israel
Fil: Burdman, Saul. The Hebrew University Of Jerusalem; Israel
Fil: Okon, Yaacov. The Hebrew University Of Jerusalem; Israel
description The Azospirillum genus comprises free-living, plant growth-promoting, nitrogen-fixing bacteria found in the rhizosphere of plant roots. Azospirilla are able to promote plant growth mainly through improvement of root development. Bacterial surface components, such as extracellular polysaccharides and proteins, are involved in root colonization. Phase variation – or phenotypic variation – is one of the mechanisms by which microorganisms adapt to environmental changes. This phenomenon is characterized by the presence of a sub-population of the bacteria presenting a different phenotype relative to the major population. In this study we characterized phenotypic variation of Azospirillum brasilense Sp7. When plated on solid media, some A. brasilense colonies were shown to possess a much more mucoid morphology, producing 7.5–8 times more exopolysaccharide with different monosaccharide composition than the parental strain Sp7. The rate of appearance of this kind of variant colonies was 1 in 5000, in agreement with the accepted rate for the phase/phenotypic variation phenomenon. The variants were significantly more resistant to heat and UV-exposure than the parental strain and displayed genomic changes as seen by the different band patterns following ERIC-PCR, BOX-PCR and RAPD analyses. In plant inoculation experiments under greenhouse conditions, with maize, wheat, soybean and peanuts, the EPS overproducing variants performed as similar as the parental strain. Therefore, EPS overproduction did not confer an apparent advantage to A. brasilense in terms of induction of plant growth promotion.
publishDate 2013
dc.date.none.fl_str_mv 2013-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24138
Volfson, Victoria; Fibach Paldi, Sharon; Paulucci, Natalia Soledad; Dardanelli, Marta Susana; Matan, Ofra; et al.; Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects; Elsevier; Soil Biology And Biochemistry; 67; 9-2013; 255-262
0038-0717
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24138
identifier_str_mv Volfson, Victoria; Fibach Paldi, Sharon; Paulucci, Natalia Soledad; Dardanelli, Marta Susana; Matan, Ofra; et al.; Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects; Elsevier; Soil Biology And Biochemistry; 67; 9-2013; 255-262
0038-0717
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.soilbio.2013.09.008
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S003807171300309X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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