Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects
- Autores
- Volfson, Victoria; Fibach Paldi, Sharon; Paulucci, Natalia Soledad; Dardanelli, Marta Susana; Matan, Ofra; Burdman, Saul; Okon, Yaacov
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The Azospirillum genus comprises free-living, plant growth-promoting, nitrogen-fixing bacteria found in the rhizosphere of plant roots. Azospirilla are able to promote plant growth mainly through improvement of root development. Bacterial surface components, such as extracellular polysaccharides and proteins, are involved in root colonization. Phase variation – or phenotypic variation – is one of the mechanisms by which microorganisms adapt to environmental changes. This phenomenon is characterized by the presence of a sub-population of the bacteria presenting a different phenotype relative to the major population. In this study we characterized phenotypic variation of Azospirillum brasilense Sp7. When plated on solid media, some A. brasilense colonies were shown to possess a much more mucoid morphology, producing 7.5–8 times more exopolysaccharide with different monosaccharide composition than the parental strain Sp7. The rate of appearance of this kind of variant colonies was 1 in 5000, in agreement with the accepted rate for the phase/phenotypic variation phenomenon. The variants were significantly more resistant to heat and UV-exposure than the parental strain and displayed genomic changes as seen by the different band patterns following ERIC-PCR, BOX-PCR and RAPD analyses. In plant inoculation experiments under greenhouse conditions, with maize, wheat, soybean and peanuts, the EPS overproducing variants performed as similar as the parental strain. Therefore, EPS overproduction did not confer an apparent advantage to A. brasilense in terms of induction of plant growth promotion.
Fil: Volfson, Victoria. The Hebrew University Of Jerusalem; Israel
Fil: Fibach Paldi, Sharon. The Hebrew University Of Jerusalem; Israel
Fil: Paulucci, Natalia Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Matan, Ofra. The Hebrew University Of Jerusalem; Israel
Fil: Burdman, Saul. The Hebrew University Of Jerusalem; Israel
Fil: Okon, Yaacov. The Hebrew University Of Jerusalem; Israel - Materia
-
Azospirillum Brasilense
Phase Variation
Plant Growth Promotion
Genomic Changes - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/24138
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Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effectsVolfson, VictoriaFibach Paldi, SharonPaulucci, Natalia SoledadDardanelli, Marta SusanaMatan, OfraBurdman, SaulOkon, YaacovAzospirillum BrasilensePhase VariationPlant Growth PromotionGenomic Changeshttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The Azospirillum genus comprises free-living, plant growth-promoting, nitrogen-fixing bacteria found in the rhizosphere of plant roots. Azospirilla are able to promote plant growth mainly through improvement of root development. Bacterial surface components, such as extracellular polysaccharides and proteins, are involved in root colonization. Phase variation – or phenotypic variation – is one of the mechanisms by which microorganisms adapt to environmental changes. This phenomenon is characterized by the presence of a sub-population of the bacteria presenting a different phenotype relative to the major population. In this study we characterized phenotypic variation of Azospirillum brasilense Sp7. When plated on solid media, some A. brasilense colonies were shown to possess a much more mucoid morphology, producing 7.5–8 times more exopolysaccharide with different monosaccharide composition than the parental strain Sp7. The rate of appearance of this kind of variant colonies was 1 in 5000, in agreement with the accepted rate for the phase/phenotypic variation phenomenon. The variants were significantly more resistant to heat and UV-exposure than the parental strain and displayed genomic changes as seen by the different band patterns following ERIC-PCR, BOX-PCR and RAPD analyses. In plant inoculation experiments under greenhouse conditions, with maize, wheat, soybean and peanuts, the EPS overproducing variants performed as similar as the parental strain. Therefore, EPS overproduction did not confer an apparent advantage to A. brasilense in terms of induction of plant growth promotion.Fil: Volfson, Victoria. The Hebrew University Of Jerusalem; IsraelFil: Fibach Paldi, Sharon. The Hebrew University Of Jerusalem; IsraelFil: Paulucci, Natalia Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Matan, Ofra. The Hebrew University Of Jerusalem; IsraelFil: Burdman, Saul. The Hebrew University Of Jerusalem; IsraelFil: Okon, Yaacov. The Hebrew University Of Jerusalem; IsraelElsevier2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24138Volfson, Victoria; Fibach Paldi, Sharon; Paulucci, Natalia Soledad; Dardanelli, Marta Susana; Matan, Ofra; et al.; Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects; Elsevier; Soil Biology And Biochemistry; 67; 9-2013; 255-2620038-0717CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.soilbio.2013.09.008info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S003807171300309Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:46Zoai:ri.conicet.gov.ar:11336/24138instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:46.573CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects |
title |
Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects |
spellingShingle |
Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects Volfson, Victoria Azospirillum Brasilense Phase Variation Plant Growth Promotion Genomic Changes |
title_short |
Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects |
title_full |
Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects |
title_fullStr |
Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects |
title_full_unstemmed |
Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects |
title_sort |
Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects |
dc.creator.none.fl_str_mv |
Volfson, Victoria Fibach Paldi, Sharon Paulucci, Natalia Soledad Dardanelli, Marta Susana Matan, Ofra Burdman, Saul Okon, Yaacov |
author |
Volfson, Victoria |
author_facet |
Volfson, Victoria Fibach Paldi, Sharon Paulucci, Natalia Soledad Dardanelli, Marta Susana Matan, Ofra Burdman, Saul Okon, Yaacov |
author_role |
author |
author2 |
Fibach Paldi, Sharon Paulucci, Natalia Soledad Dardanelli, Marta Susana Matan, Ofra Burdman, Saul Okon, Yaacov |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Azospirillum Brasilense Phase Variation Plant Growth Promotion Genomic Changes |
topic |
Azospirillum Brasilense Phase Variation Plant Growth Promotion Genomic Changes |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.1 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
The Azospirillum genus comprises free-living, plant growth-promoting, nitrogen-fixing bacteria found in the rhizosphere of plant roots. Azospirilla are able to promote plant growth mainly through improvement of root development. Bacterial surface components, such as extracellular polysaccharides and proteins, are involved in root colonization. Phase variation – or phenotypic variation – is one of the mechanisms by which microorganisms adapt to environmental changes. This phenomenon is characterized by the presence of a sub-population of the bacteria presenting a different phenotype relative to the major population. In this study we characterized phenotypic variation of Azospirillum brasilense Sp7. When plated on solid media, some A. brasilense colonies were shown to possess a much more mucoid morphology, producing 7.5–8 times more exopolysaccharide with different monosaccharide composition than the parental strain Sp7. The rate of appearance of this kind of variant colonies was 1 in 5000, in agreement with the accepted rate for the phase/phenotypic variation phenomenon. The variants were significantly more resistant to heat and UV-exposure than the parental strain and displayed genomic changes as seen by the different band patterns following ERIC-PCR, BOX-PCR and RAPD analyses. In plant inoculation experiments under greenhouse conditions, with maize, wheat, soybean and peanuts, the EPS overproducing variants performed as similar as the parental strain. Therefore, EPS overproduction did not confer an apparent advantage to A. brasilense in terms of induction of plant growth promotion. Fil: Volfson, Victoria. The Hebrew University Of Jerusalem; Israel Fil: Fibach Paldi, Sharon. The Hebrew University Of Jerusalem; Israel Fil: Paulucci, Natalia Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Matan, Ofra. The Hebrew University Of Jerusalem; Israel Fil: Burdman, Saul. The Hebrew University Of Jerusalem; Israel Fil: Okon, Yaacov. The Hebrew University Of Jerusalem; Israel |
description |
The Azospirillum genus comprises free-living, plant growth-promoting, nitrogen-fixing bacteria found in the rhizosphere of plant roots. Azospirilla are able to promote plant growth mainly through improvement of root development. Bacterial surface components, such as extracellular polysaccharides and proteins, are involved in root colonization. Phase variation – or phenotypic variation – is one of the mechanisms by which microorganisms adapt to environmental changes. This phenomenon is characterized by the presence of a sub-population of the bacteria presenting a different phenotype relative to the major population. In this study we characterized phenotypic variation of Azospirillum brasilense Sp7. When plated on solid media, some A. brasilense colonies were shown to possess a much more mucoid morphology, producing 7.5–8 times more exopolysaccharide with different monosaccharide composition than the parental strain Sp7. The rate of appearance of this kind of variant colonies was 1 in 5000, in agreement with the accepted rate for the phase/phenotypic variation phenomenon. The variants were significantly more resistant to heat and UV-exposure than the parental strain and displayed genomic changes as seen by the different band patterns following ERIC-PCR, BOX-PCR and RAPD analyses. In plant inoculation experiments under greenhouse conditions, with maize, wheat, soybean and peanuts, the EPS overproducing variants performed as similar as the parental strain. Therefore, EPS overproduction did not confer an apparent advantage to A. brasilense in terms of induction of plant growth promotion. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/24138 Volfson, Victoria; Fibach Paldi, Sharon; Paulucci, Natalia Soledad; Dardanelli, Marta Susana; Matan, Ofra; et al.; Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects; Elsevier; Soil Biology And Biochemistry; 67; 9-2013; 255-262 0038-0717 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/24138 |
identifier_str_mv |
Volfson, Victoria; Fibach Paldi, Sharon; Paulucci, Natalia Soledad; Dardanelli, Marta Susana; Matan, Ofra; et al.; Phenotypic variation in Azospirillum brasilense Sp7 does not influence plant growth promotion effects; Elsevier; Soil Biology And Biochemistry; 67; 9-2013; 255-262 0038-0717 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.soilbio.2013.09.008 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S003807171300309X |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613378027290624 |
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13.070432 |