Genome-based reclassification of azospirillum brasilense SP245 as the type strain of azospirillum baldaniorum sp. nov
- Autores
- Ferreira, Natalia Dos Santos; Sant´Anna, Fernando Hayashi; Reis, Veronica Massena; Ambrosini, Adriana; Volpiano, Camila Gazolla; Rothballer, Michael; Schwab, Stefan; Baura, Valter Antonio; Balsanelli, Eduardo; Pedrosa, Fabio de Oliveira; Passaglia, Luciane Maria Pereira; de Souza, Emanuel Maltempi; Hartmann, Anton; Cassan, Fabricio Dario; Zilli, Jerri Edson
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Azospirillum sp. strain Sp245T, originally identified as belonging to Azospirillum brasilense, is recognized as a plant-growth-promoting rhizobacterium due to its ability to fix atmospheric nitrogen and to produce plant-beneficial compounds. Azospirillum sp. Sp245T and other related strains were isolated from the root surfaces of different plants in Brazil. Cells are Gram-negative, curved or slightly curved rods, and motile with polar and lateral flagella. Their growth temperature varies between 20 to 38 °C and their carbon source utilization is similar to other Azospirillum species. A preliminary 16S rRNA sequence analysis showed that the new species is closely related to A. brasilense Sp7T and A. formosense CC-Nfb-7T. Housekeeping genes revealed that Azospirillum sp. Sp245T, BR 12001 and Vi22 form a separate cluster from strain A. formosense CC-Nfb-7T, and a group of strains closely related to A. brasilense Sp7T. Overall genome relatedness index (OGRI) analyses estimated based on average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) between Azospirillum sp. Sp245T and its close relatives to other Azospirillum species type strains, such as A. brasilense Sp7T and A. formosense CC-Nfb-7T, revealed values lower than the limit of species circumscription. Moreover, core-proteome phylogeny including 1079 common shared proteins showed the independent clusterization of A. brasilense Sp7T, A. formosense CC-Nfb-7T and Azospirillum sp. Sp245T, a finding that was corroborated by the genome clustering of OGRI values and housekeeping phylogenies. The DNA G+C content of the cluster of Sp245T was 68.4–68.6%. Based on the phylogenetic, genomic, phenotypical and physiological analysis, we propose that strain Sp245T together with the strains Vi22 and BR12001 represent a novel species of the genus Azospirillum, for which the name Azospirillum baldaniorum sp. nov. is proposed. The type strain is Sp245T (=BR 11005T=IBPPM 219T) (GCF_007827915.1, GCF_000237365.1, and GCF_003119195.2).
Fil: Ferreira, Natalia Dos Santos. Universidade Federal Rural Do Rio de Janeiro; Brasil
Fil: Sant´Anna, Fernando Hayashi. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Reis, Veronica Massena. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Ambrosini, Adriana. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Volpiano, Camila Gazolla. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Rothballer, Michael. Helmholtz Center Munich German Research Center For Environmental Health; Alemania
Fil: Schwab, Stefan. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Baura, Valter Antonio. Universidade Federal do Paraná; Brasil
Fil: Balsanelli, Eduardo. Universidade Federal do Paraná; Brasil
Fil: Pedrosa, Fabio de Oliveira. Universidade Federal do Paraná; Brasil
Fil: Passaglia, Luciane Maria Pereira. Universidade Federal do Rio Grande do Sul; Brasil
Fil: de Souza, Emanuel Maltempi. Universidade Federal do Paraná; Brasil
Fil: Hartmann, Anton. Ludwig Maximilians Universitat; Alemania
Fil: Cassan, Fabricio Dario. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Zilli, Jerri Edson. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil - Materia
-
AZOSPIRILLUM
AZOSPIRILLUM BRASILENSE SP7
GENOMIC METRICS
HOUSEKEEPING PHYLOGENIES
PROTEOMA-CORE PHYLOGENY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/141960
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
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Genome-based reclassification of azospirillum brasilense SP245 as the type strain of azospirillum baldaniorum sp. novFerreira, Natalia Dos SantosSant´Anna, Fernando HayashiReis, Veronica MassenaAmbrosini, AdrianaVolpiano, Camila GazollaRothballer, MichaelSchwab, StefanBaura, Valter AntonioBalsanelli, EduardoPedrosa, Fabio de OliveiraPassaglia, Luciane Maria Pereirade Souza, Emanuel MaltempiHartmann, AntonCassan, Fabricio DarioZilli, Jerri EdsonAZOSPIRILLUMAZOSPIRILLUM BRASILENSE SP7GENOMIC METRICSHOUSEKEEPING PHYLOGENIESPROTEOMA-CORE PHYLOGENYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Azospirillum sp. strain Sp245T, originally identified as belonging to Azospirillum brasilense, is recognized as a plant-growth-promoting rhizobacterium due to its ability to fix atmospheric nitrogen and to produce plant-beneficial compounds. Azospirillum sp. Sp245T and other related strains were isolated from the root surfaces of different plants in Brazil. Cells are Gram-negative, curved or slightly curved rods, and motile with polar and lateral flagella. Their growth temperature varies between 20 to 38 °C and their carbon source utilization is similar to other Azospirillum species. A preliminary 16S rRNA sequence analysis showed that the new species is closely related to A. brasilense Sp7T and A. formosense CC-Nfb-7T. Housekeeping genes revealed that Azospirillum sp. Sp245T, BR 12001 and Vi22 form a separate cluster from strain A. formosense CC-Nfb-7T, and a group of strains closely related to A. brasilense Sp7T. Overall genome relatedness index (OGRI) analyses estimated based on average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) between Azospirillum sp. Sp245T and its close relatives to other Azospirillum species type strains, such as A. brasilense Sp7T and A. formosense CC-Nfb-7T, revealed values lower than the limit of species circumscription. Moreover, core-proteome phylogeny including 1079 common shared proteins showed the independent clusterization of A. brasilense Sp7T, A. formosense CC-Nfb-7T and Azospirillum sp. Sp245T, a finding that was corroborated by the genome clustering of OGRI values and housekeeping phylogenies. The DNA G+C content of the cluster of Sp245T was 68.4–68.6%. Based on the phylogenetic, genomic, phenotypical and physiological analysis, we propose that strain Sp245T together with the strains Vi22 and BR12001 represent a novel species of the genus Azospirillum, for which the name Azospirillum baldaniorum sp. nov. is proposed. The type strain is Sp245T (=BR 11005T=IBPPM 219T) (GCF_007827915.1, GCF_000237365.1, and GCF_003119195.2).Fil: Ferreira, Natalia Dos Santos. Universidade Federal Rural Do Rio de Janeiro; BrasilFil: Sant´Anna, Fernando Hayashi. Universidade Federal do Rio Grande do Sul; BrasilFil: Reis, Veronica Massena. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Ambrosini, Adriana. Universidade Federal do Rio Grande do Sul; BrasilFil: Volpiano, Camila Gazolla. Universidade Federal do Rio Grande do Sul; BrasilFil: Rothballer, Michael. Helmholtz Center Munich German Research Center For Environmental Health; AlemaniaFil: Schwab, Stefan. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Baura, Valter Antonio. Universidade Federal do Paraná; BrasilFil: Balsanelli, Eduardo. Universidade Federal do Paraná; BrasilFil: Pedrosa, Fabio de Oliveira. Universidade Federal do Paraná; BrasilFil: Passaglia, Luciane Maria Pereira. Universidade Federal do Rio Grande do Sul; BrasilFil: de Souza, Emanuel Maltempi. Universidade Federal do Paraná; BrasilFil: Hartmann, Anton. Ludwig Maximilians Universitat; AlemaniaFil: Cassan, Fabricio Dario. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Zilli, Jerri Edson. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilSociety for General Microbiology2020-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/141960Ferreira, Natalia Dos Santos; Sant´Anna, Fernando Hayashi; Reis, Veronica Massena; Ambrosini, Adriana; Volpiano, Camila Gazolla; et al.; Genome-based reclassification of azospirillum brasilense SP245 as the type strain of azospirillum baldaniorum sp. nov; Society for General Microbiology; International Journal of Systematic and Evolutionary Microbiology; 70; 12; 10-2020; 6203-62121466-50261466-5034CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/ 10.1099/ijsem.0.004517info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004517info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:10Zoai:ri.conicet.gov.ar:11336/141960instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:10.565CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Genome-based reclassification of azospirillum brasilense SP245 as the type strain of azospirillum baldaniorum sp. nov |
| title |
Genome-based reclassification of azospirillum brasilense SP245 as the type strain of azospirillum baldaniorum sp. nov |
| spellingShingle |
Genome-based reclassification of azospirillum brasilense SP245 as the type strain of azospirillum baldaniorum sp. nov Ferreira, Natalia Dos Santos AZOSPIRILLUM AZOSPIRILLUM BRASILENSE SP7 GENOMIC METRICS HOUSEKEEPING PHYLOGENIES PROTEOMA-CORE PHYLOGENY |
| title_short |
Genome-based reclassification of azospirillum brasilense SP245 as the type strain of azospirillum baldaniorum sp. nov |
| title_full |
Genome-based reclassification of azospirillum brasilense SP245 as the type strain of azospirillum baldaniorum sp. nov |
| title_fullStr |
Genome-based reclassification of azospirillum brasilense SP245 as the type strain of azospirillum baldaniorum sp. nov |
| title_full_unstemmed |
Genome-based reclassification of azospirillum brasilense SP245 as the type strain of azospirillum baldaniorum sp. nov |
| title_sort |
Genome-based reclassification of azospirillum brasilense SP245 as the type strain of azospirillum baldaniorum sp. nov |
| dc.creator.none.fl_str_mv |
Ferreira, Natalia Dos Santos Sant´Anna, Fernando Hayashi Reis, Veronica Massena Ambrosini, Adriana Volpiano, Camila Gazolla Rothballer, Michael Schwab, Stefan Baura, Valter Antonio Balsanelli, Eduardo Pedrosa, Fabio de Oliveira Passaglia, Luciane Maria Pereira de Souza, Emanuel Maltempi Hartmann, Anton Cassan, Fabricio Dario Zilli, Jerri Edson |
| author |
Ferreira, Natalia Dos Santos |
| author_facet |
Ferreira, Natalia Dos Santos Sant´Anna, Fernando Hayashi Reis, Veronica Massena Ambrosini, Adriana Volpiano, Camila Gazolla Rothballer, Michael Schwab, Stefan Baura, Valter Antonio Balsanelli, Eduardo Pedrosa, Fabio de Oliveira Passaglia, Luciane Maria Pereira de Souza, Emanuel Maltempi Hartmann, Anton Cassan, Fabricio Dario Zilli, Jerri Edson |
| author_role |
author |
| author2 |
Sant´Anna, Fernando Hayashi Reis, Veronica Massena Ambrosini, Adriana Volpiano, Camila Gazolla Rothballer, Michael Schwab, Stefan Baura, Valter Antonio Balsanelli, Eduardo Pedrosa, Fabio de Oliveira Passaglia, Luciane Maria Pereira de Souza, Emanuel Maltempi Hartmann, Anton Cassan, Fabricio Dario Zilli, Jerri Edson |
| author2_role |
author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
AZOSPIRILLUM AZOSPIRILLUM BRASILENSE SP7 GENOMIC METRICS HOUSEKEEPING PHYLOGENIES PROTEOMA-CORE PHYLOGENY |
| topic |
AZOSPIRILLUM AZOSPIRILLUM BRASILENSE SP7 GENOMIC METRICS HOUSEKEEPING PHYLOGENIES PROTEOMA-CORE PHYLOGENY |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Azospirillum sp. strain Sp245T, originally identified as belonging to Azospirillum brasilense, is recognized as a plant-growth-promoting rhizobacterium due to its ability to fix atmospheric nitrogen and to produce plant-beneficial compounds. Azospirillum sp. Sp245T and other related strains were isolated from the root surfaces of different plants in Brazil. Cells are Gram-negative, curved or slightly curved rods, and motile with polar and lateral flagella. Their growth temperature varies between 20 to 38 °C and their carbon source utilization is similar to other Azospirillum species. A preliminary 16S rRNA sequence analysis showed that the new species is closely related to A. brasilense Sp7T and A. formosense CC-Nfb-7T. Housekeeping genes revealed that Azospirillum sp. Sp245T, BR 12001 and Vi22 form a separate cluster from strain A. formosense CC-Nfb-7T, and a group of strains closely related to A. brasilense Sp7T. Overall genome relatedness index (OGRI) analyses estimated based on average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) between Azospirillum sp. Sp245T and its close relatives to other Azospirillum species type strains, such as A. brasilense Sp7T and A. formosense CC-Nfb-7T, revealed values lower than the limit of species circumscription. Moreover, core-proteome phylogeny including 1079 common shared proteins showed the independent clusterization of A. brasilense Sp7T, A. formosense CC-Nfb-7T and Azospirillum sp. Sp245T, a finding that was corroborated by the genome clustering of OGRI values and housekeeping phylogenies. The DNA G+C content of the cluster of Sp245T was 68.4–68.6%. Based on the phylogenetic, genomic, phenotypical and physiological analysis, we propose that strain Sp245T together with the strains Vi22 and BR12001 represent a novel species of the genus Azospirillum, for which the name Azospirillum baldaniorum sp. nov. is proposed. The type strain is Sp245T (=BR 11005T=IBPPM 219T) (GCF_007827915.1, GCF_000237365.1, and GCF_003119195.2). Fil: Ferreira, Natalia Dos Santos. Universidade Federal Rural Do Rio de Janeiro; Brasil Fil: Sant´Anna, Fernando Hayashi. Universidade Federal do Rio Grande do Sul; Brasil Fil: Reis, Veronica Massena. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Ambrosini, Adriana. Universidade Federal do Rio Grande do Sul; Brasil Fil: Volpiano, Camila Gazolla. Universidade Federal do Rio Grande do Sul; Brasil Fil: Rothballer, Michael. Helmholtz Center Munich German Research Center For Environmental Health; Alemania Fil: Schwab, Stefan. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Baura, Valter Antonio. Universidade Federal do Paraná; Brasil Fil: Balsanelli, Eduardo. Universidade Federal do Paraná; Brasil Fil: Pedrosa, Fabio de Oliveira. Universidade Federal do Paraná; Brasil Fil: Passaglia, Luciane Maria Pereira. Universidade Federal do Rio Grande do Sul; Brasil Fil: de Souza, Emanuel Maltempi. Universidade Federal do Paraná; Brasil Fil: Hartmann, Anton. Ludwig Maximilians Universitat; Alemania Fil: Cassan, Fabricio Dario. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; Argentina Fil: Zilli, Jerri Edson. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil |
| description |
Azospirillum sp. strain Sp245T, originally identified as belonging to Azospirillum brasilense, is recognized as a plant-growth-promoting rhizobacterium due to its ability to fix atmospheric nitrogen and to produce plant-beneficial compounds. Azospirillum sp. Sp245T and other related strains were isolated from the root surfaces of different plants in Brazil. Cells are Gram-negative, curved or slightly curved rods, and motile with polar and lateral flagella. Their growth temperature varies between 20 to 38 °C and their carbon source utilization is similar to other Azospirillum species. A preliminary 16S rRNA sequence analysis showed that the new species is closely related to A. brasilense Sp7T and A. formosense CC-Nfb-7T. Housekeeping genes revealed that Azospirillum sp. Sp245T, BR 12001 and Vi22 form a separate cluster from strain A. formosense CC-Nfb-7T, and a group of strains closely related to A. brasilense Sp7T. Overall genome relatedness index (OGRI) analyses estimated based on average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) between Azospirillum sp. Sp245T and its close relatives to other Azospirillum species type strains, such as A. brasilense Sp7T and A. formosense CC-Nfb-7T, revealed values lower than the limit of species circumscription. Moreover, core-proteome phylogeny including 1079 common shared proteins showed the independent clusterization of A. brasilense Sp7T, A. formosense CC-Nfb-7T and Azospirillum sp. Sp245T, a finding that was corroborated by the genome clustering of OGRI values and housekeeping phylogenies. The DNA G+C content of the cluster of Sp245T was 68.4–68.6%. Based on the phylogenetic, genomic, phenotypical and physiological analysis, we propose that strain Sp245T together with the strains Vi22 and BR12001 represent a novel species of the genus Azospirillum, for which the name Azospirillum baldaniorum sp. nov. is proposed. The type strain is Sp245T (=BR 11005T=IBPPM 219T) (GCF_007827915.1, GCF_000237365.1, and GCF_003119195.2). |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-10 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/141960 Ferreira, Natalia Dos Santos; Sant´Anna, Fernando Hayashi; Reis, Veronica Massena; Ambrosini, Adriana; Volpiano, Camila Gazolla; et al.; Genome-based reclassification of azospirillum brasilense SP245 as the type strain of azospirillum baldaniorum sp. nov; Society for General Microbiology; International Journal of Systematic and Evolutionary Microbiology; 70; 12; 10-2020; 6203-6212 1466-5026 1466-5034 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/141960 |
| identifier_str_mv |
Ferreira, Natalia Dos Santos; Sant´Anna, Fernando Hayashi; Reis, Veronica Massena; Ambrosini, Adriana; Volpiano, Camila Gazolla; et al.; Genome-based reclassification of azospirillum brasilense SP245 as the type strain of azospirillum baldaniorum sp. nov; Society for General Microbiology; International Journal of Systematic and Evolutionary Microbiology; 70; 12; 10-2020; 6203-6212 1466-5026 1466-5034 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/ 10.1099/ijsem.0.004517 info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004517 |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf |
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Society for General Microbiology |
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Society for General Microbiology |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613689099943936 |
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13.069144 |