Co-exposure of the organic nanomaterial fullerene C60 with benzo[a]pyrene in Danio rerio (zebrafish) hepatocytes: Evidence of toxicological interactions

Autores
Ribas Ferreira, Josencler L.; Lonné, María Noelia; França, Thiago A.; Maximilla, Naiana R.; Lugokenski, Thiago H.; Costa, Patrícia G.; Fillmann, Gilberto; Soares, Félix A.; de la Torre, Fernando Roman; Monserrat, José María
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Compounds from the nanotechnology industry, such as carbon-based nanomaterials, are strong candidates to contaminate aquatic environments because their production and disposal have exponentially grown in a few years. Previous evidence shows that fullerene C60, a carbon nanomaterial, can facilitate the intake of metals or PAHs both in vivo and in vitro, potentially amplifying the deleterious effects of these toxicants in organisms. The present work aimed to investigate the effects of fullerene C60 in a Danio rerio (zebrafish) hepatocyte cell lineage exposed to benzo[a]pyrene (BaP) in terms of cell viability, oxidative stress parameters and BaP intracellular accumulation. Additionally, a computational docking was performed to investigate the interaction of the fullerene C60 molecule with the detoxificatory and antioxidant enzyme πGST. Fullerene C60 provoked a significant (p < 0.05) loss in cellular viability when co-exposed with BaP at 0.01, 0.1 and 1.0 μg/L, and induced an increase (p < 0.05) in BaP accumulation in the cells after 3 and 4 h of exposure. The levels of reactive oxygen species (ROS) in the cells exposed to BaP were diminished (p < 0.05) by the fullerene addition, and the increase of the GST activity observed in the BaP-only treated cells was reduced to the basal levels by co-exposure to fullerene. However, despite the potential of the fullerene molecule to inhibit π GST activity, demonstrated by the computational docking, the nanomaterial did not significantly (p > 0.05) alter the enzyme activity when added to GST purified extracts from the zebrafish hepatocyte cells. These results show that fullerene C60 can increase the intake of BaP into the cells, decreasing cell viability and impairing the detoxificatory response by phase II enzymes, such as GST, and this latter effect should be occurring at the transcriptional level.
Fil: Ribas Ferreira, Josencler L.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Lonné, María Noelia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: França, Thiago A.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Maximilla, Naiana R.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Lugokenski, Thiago H.. Universidade Federal de Santa Maria. Departamento de Química; Brasil
Fil: Costa, Patrícia G.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Fillmann, Gilberto. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Soares, Félix A.. Universidade Federal de Santa Maria. Departamento de Química; Brasil
Fil: de la Torre, Fernando Roman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Monserrat, José María. Universidade Federal do Rio Grande do Sul; Brasil. Instituto Nacional de Ciência e Tecnologia de Nanomateriais de Carbono; Brasil
Materia
NANOTOXICOLOGIA
FULERENO
CULTIVO CELULAR
GLUTATIONA-S-TRANSFERASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33301
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/33301
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Co-exposure of the organic nanomaterial fullerene C60 with benzo[a]pyrene in Danio rerio (zebrafish) hepatocytes: Evidence of toxicological interactionsRibas Ferreira, Josencler L.Lonné, María NoeliaFrança, Thiago A.Maximilla, Naiana R.Lugokenski, Thiago H.Costa, Patrícia G.Fillmann, GilbertoSoares, Félix A.de la Torre, Fernando RomanMonserrat, José MaríaNANOTOXICOLOGIAFULERENOCULTIVO CELULARGLUTATIONA-S-TRANSFERASEhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Compounds from the nanotechnology industry, such as carbon-based nanomaterials, are strong candidates to contaminate aquatic environments because their production and disposal have exponentially grown in a few years. Previous evidence shows that fullerene C60, a carbon nanomaterial, can facilitate the intake of metals or PAHs both in vivo and in vitro, potentially amplifying the deleterious effects of these toxicants in organisms. The present work aimed to investigate the effects of fullerene C60 in a Danio rerio (zebrafish) hepatocyte cell lineage exposed to benzo[a]pyrene (BaP) in terms of cell viability, oxidative stress parameters and BaP intracellular accumulation. Additionally, a computational docking was performed to investigate the interaction of the fullerene C60 molecule with the detoxificatory and antioxidant enzyme πGST. Fullerene C60 provoked a significant (p < 0.05) loss in cellular viability when co-exposed with BaP at 0.01, 0.1 and 1.0 μg/L, and induced an increase (p < 0.05) in BaP accumulation in the cells after 3 and 4 h of exposure. The levels of reactive oxygen species (ROS) in the cells exposed to BaP were diminished (p < 0.05) by the fullerene addition, and the increase of the GST activity observed in the BaP-only treated cells was reduced to the basal levels by co-exposure to fullerene. However, despite the potential of the fullerene molecule to inhibit π GST activity, demonstrated by the computational docking, the nanomaterial did not significantly (p > 0.05) alter the enzyme activity when added to GST purified extracts from the zebrafish hepatocyte cells. These results show that fullerene C60 can increase the intake of BaP into the cells, decreasing cell viability and impairing the detoxificatory response by phase II enzymes, such as GST, and this latter effect should be occurring at the transcriptional level.Fil: Ribas Ferreira, Josencler L.. Universidade Federal do Rio Grande do Sul; BrasilFil: Lonné, María Noelia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: França, Thiago A.. Universidade Federal do Rio Grande do Sul; BrasilFil: Maximilla, Naiana R.. Universidade Federal do Rio Grande do Sul; BrasilFil: Lugokenski, Thiago H.. Universidade Federal de Santa Maria. Departamento de Química; BrasilFil: Costa, Patrícia G.. Universidade Federal do Rio Grande do Sul; BrasilFil: Fillmann, Gilberto. Universidade Federal do Rio Grande do Sul; BrasilFil: Soares, Félix A.. Universidade Federal de Santa Maria. Departamento de Química; BrasilFil: de la Torre, Fernando Roman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaFil: Monserrat, José María. Universidade Federal do Rio Grande do Sul; Brasil. Instituto Nacional de Ciência e Tecnologia de Nanomateriais de Carbono; BrasilElsevier2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33301Fillmann, Gilberto; Lonné, María Noelia; Maximilla, Naiana R.; França, Thiago A.; de la Torre, Fernando Roman; Monserrat, José María; et al.; Co-exposure of the organic nanomaterial fullerene C60 with benzo[a]pyrene in Danio rerio (zebrafish) hepatocytes: Evidence of toxicological interactions; Elsevier; Aquatic Toxicology; 147; 12-2013; 76-830166-445XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166445X13003482info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2013.12.007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:21Zoai:ri.conicet.gov.ar:11336/33301instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:21.968CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Co-exposure of the organic nanomaterial fullerene C60 with benzo[a]pyrene in Danio rerio (zebrafish) hepatocytes: Evidence of toxicological interactions
title Co-exposure of the organic nanomaterial fullerene C60 with benzo[a]pyrene in Danio rerio (zebrafish) hepatocytes: Evidence of toxicological interactions
spellingShingle Co-exposure of the organic nanomaterial fullerene C60 with benzo[a]pyrene in Danio rerio (zebrafish) hepatocytes: Evidence of toxicological interactions
Ribas Ferreira, Josencler L.
NANOTOXICOLOGIA
FULERENO
CULTIVO CELULAR
GLUTATIONA-S-TRANSFERASE
title_short Co-exposure of the organic nanomaterial fullerene C60 with benzo[a]pyrene in Danio rerio (zebrafish) hepatocytes: Evidence of toxicological interactions
title_full Co-exposure of the organic nanomaterial fullerene C60 with benzo[a]pyrene in Danio rerio (zebrafish) hepatocytes: Evidence of toxicological interactions
title_fullStr Co-exposure of the organic nanomaterial fullerene C60 with benzo[a]pyrene in Danio rerio (zebrafish) hepatocytes: Evidence of toxicological interactions
title_full_unstemmed Co-exposure of the organic nanomaterial fullerene C60 with benzo[a]pyrene in Danio rerio (zebrafish) hepatocytes: Evidence of toxicological interactions
title_sort Co-exposure of the organic nanomaterial fullerene C60 with benzo[a]pyrene in Danio rerio (zebrafish) hepatocytes: Evidence of toxicological interactions
dc.creator.none.fl_str_mv Ribas Ferreira, Josencler L.
Lonné, María Noelia
França, Thiago A.
Maximilla, Naiana R.
Lugokenski, Thiago H.
Costa, Patrícia G.
Fillmann, Gilberto
Soares, Félix A.
de la Torre, Fernando Roman
Monserrat, José María
author Ribas Ferreira, Josencler L.
author_facet Ribas Ferreira, Josencler L.
Lonné, María Noelia
França, Thiago A.
Maximilla, Naiana R.
Lugokenski, Thiago H.
Costa, Patrícia G.
Fillmann, Gilberto
Soares, Félix A.
de la Torre, Fernando Roman
Monserrat, José María
author_role author
author2 Lonné, María Noelia
França, Thiago A.
Maximilla, Naiana R.
Lugokenski, Thiago H.
Costa, Patrícia G.
Fillmann, Gilberto
Soares, Félix A.
de la Torre, Fernando Roman
Monserrat, José María
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv NANOTOXICOLOGIA
FULERENO
CULTIVO CELULAR
GLUTATIONA-S-TRANSFERASE
topic NANOTOXICOLOGIA
FULERENO
CULTIVO CELULAR
GLUTATIONA-S-TRANSFERASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Compounds from the nanotechnology industry, such as carbon-based nanomaterials, are strong candidates to contaminate aquatic environments because their production and disposal have exponentially grown in a few years. Previous evidence shows that fullerene C60, a carbon nanomaterial, can facilitate the intake of metals or PAHs both in vivo and in vitro, potentially amplifying the deleterious effects of these toxicants in organisms. The present work aimed to investigate the effects of fullerene C60 in a Danio rerio (zebrafish) hepatocyte cell lineage exposed to benzo[a]pyrene (BaP) in terms of cell viability, oxidative stress parameters and BaP intracellular accumulation. Additionally, a computational docking was performed to investigate the interaction of the fullerene C60 molecule with the detoxificatory and antioxidant enzyme πGST. Fullerene C60 provoked a significant (p < 0.05) loss in cellular viability when co-exposed with BaP at 0.01, 0.1 and 1.0 μg/L, and induced an increase (p < 0.05) in BaP accumulation in the cells after 3 and 4 h of exposure. The levels of reactive oxygen species (ROS) in the cells exposed to BaP were diminished (p < 0.05) by the fullerene addition, and the increase of the GST activity observed in the BaP-only treated cells was reduced to the basal levels by co-exposure to fullerene. However, despite the potential of the fullerene molecule to inhibit π GST activity, demonstrated by the computational docking, the nanomaterial did not significantly (p > 0.05) alter the enzyme activity when added to GST purified extracts from the zebrafish hepatocyte cells. These results show that fullerene C60 can increase the intake of BaP into the cells, decreasing cell viability and impairing the detoxificatory response by phase II enzymes, such as GST, and this latter effect should be occurring at the transcriptional level.
Fil: Ribas Ferreira, Josencler L.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Lonné, María Noelia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: França, Thiago A.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Maximilla, Naiana R.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Lugokenski, Thiago H.. Universidade Federal de Santa Maria. Departamento de Química; Brasil
Fil: Costa, Patrícia G.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Fillmann, Gilberto. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Soares, Félix A.. Universidade Federal de Santa Maria. Departamento de Química; Brasil
Fil: de la Torre, Fernando Roman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Monserrat, José María. Universidade Federal do Rio Grande do Sul; Brasil. Instituto Nacional de Ciência e Tecnologia de Nanomateriais de Carbono; Brasil
description Compounds from the nanotechnology industry, such as carbon-based nanomaterials, are strong candidates to contaminate aquatic environments because their production and disposal have exponentially grown in a few years. Previous evidence shows that fullerene C60, a carbon nanomaterial, can facilitate the intake of metals or PAHs both in vivo and in vitro, potentially amplifying the deleterious effects of these toxicants in organisms. The present work aimed to investigate the effects of fullerene C60 in a Danio rerio (zebrafish) hepatocyte cell lineage exposed to benzo[a]pyrene (BaP) in terms of cell viability, oxidative stress parameters and BaP intracellular accumulation. Additionally, a computational docking was performed to investigate the interaction of the fullerene C60 molecule with the detoxificatory and antioxidant enzyme πGST. Fullerene C60 provoked a significant (p < 0.05) loss in cellular viability when co-exposed with BaP at 0.01, 0.1 and 1.0 μg/L, and induced an increase (p < 0.05) in BaP accumulation in the cells after 3 and 4 h of exposure. The levels of reactive oxygen species (ROS) in the cells exposed to BaP were diminished (p < 0.05) by the fullerene addition, and the increase of the GST activity observed in the BaP-only treated cells was reduced to the basal levels by co-exposure to fullerene. However, despite the potential of the fullerene molecule to inhibit π GST activity, demonstrated by the computational docking, the nanomaterial did not significantly (p > 0.05) alter the enzyme activity when added to GST purified extracts from the zebrafish hepatocyte cells. These results show that fullerene C60 can increase the intake of BaP into the cells, decreasing cell viability and impairing the detoxificatory response by phase II enzymes, such as GST, and this latter effect should be occurring at the transcriptional level.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33301
Fillmann, Gilberto; Lonné, María Noelia; Maximilla, Naiana R.; França, Thiago A.; de la Torre, Fernando Roman; Monserrat, José María; et al.; Co-exposure of the organic nanomaterial fullerene C60 with benzo[a]pyrene in Danio rerio (zebrafish) hepatocytes: Evidence of toxicological interactions; Elsevier; Aquatic Toxicology; 147; 12-2013; 76-83
0166-445X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33301
identifier_str_mv Fillmann, Gilberto; Lonné, María Noelia; Maximilla, Naiana R.; França, Thiago A.; de la Torre, Fernando Roman; Monserrat, José María; et al.; Co-exposure of the organic nanomaterial fullerene C60 with benzo[a]pyrene in Danio rerio (zebrafish) hepatocytes: Evidence of toxicological interactions; Elsevier; Aquatic Toxicology; 147; 12-2013; 76-83
0166-445X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166445X13003482
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2013.12.007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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