Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study

Autores
Parker, Matthew; Hillman, Courtney; Fontana, Barbara; Amstislavskaya, Tamara; Gorbunova, Maria; Altenhofen, Stefani; Barthelson, Karissa; Bastos, Leonardo; Borba, João; Bonan, Carla; Brennan, Caroline; Farias Cea, Amaury; Cooper, Austin; Corcoran, Jamie; Dondossola, Eduardo; Martinez Duran, Luis; Gallas Lopes, Matheus; Galstyan, David; Garcia, Ella; Gerken, Ewan; Hindges, Robert; Kenney, Justin; Kleshchev, Maxim; Kolesnikov, Tatiana; Leggieri, Adele; Khatsko, Sergey; Lardelli, Michael; Lodetti, Guilherme; Lombardelli, Giulia; Medan, Violeta; Machado Moutinho, Lirane
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The reproducibility crisis in bioscience, characterized by inconsistent study results, impedes our understanding of biological processes and global collaborative studies offer a unique solution. This study is the first global collaboration using the zebrafish (Danio rerio) novel tank test, a behavioral assay for anxiety-like responses. We analyzed data from 20 laboratories worldwide, focusing on housing conditions and experimental setups. Our study included 488 adult zebrafish, tested for 5 min, focusing on a variety of variables. Key findings show females exhibit more anxiety-like behavior than males, underscoring sex as a critical variable. Housing conditions, including higher stocking densities and specific feed types, influenced anxiety levels. Optimal conditions (5 fish/L) and nutritionally rich feeds (e.g., rotifers), mitigated anxiety-like behaviors. Environmental stressors, like noise and transportation, significantly impacted behavior. We recommend standardizing protocols to account for sex differences, optimal stocking densities, nutritionally rich feeds, and minimizing stressors to improve zebrafish behavioral study reliability.
Fil: Parker, Matthew. University of Surrey; Reino Unido
Fil: Hillman, Courtney. University Of Surrey. Faculty Of Health And Medical Sciences.; Reino Unido
Fil: Fontana, Barbara. Universidade Federal de Santa Maria; Brasil
Fil: Amstislavskaya, Tamara. Novosibirsk State University; Rusia
Fil: Gorbunova, Maria. Ural Federal University; Rusia
Fil: Altenhofen, Stefani. Pontificia Universidade Católica do Rio Grande do Sul; Brasil
Fil: Barthelson, Karissa. University of Adelaide; Australia
Fil: Bastos, Leonardo. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Borba, João. Universidade Federal de Santa Maria; Brasil
Fil: Bonan, Carla. Pontificia Universidade Católica do Rio Grande do Sul; Brasil
Fil: Brennan, Caroline. No especifíca;
Fil: Farias Cea, Amaury. Universidad de La Frontera; Chile
Fil: Cooper, Austin. University of Surrey; Reino Unido
Fil: Corcoran, Jamie. University of Nebraska; Estados Unidos
Fil: Dondossola, Eduardo. University of Southern Santa Catarina; Brasil
Fil: Martinez Duran, Luis. Universidad de La Frontera; Chile
Fil: Gallas Lopes, Matheus. No especifíca;
Fil: Galstyan, David. St. Petersburg State University; Rusia
Fil: Garcia, Ella. Indiana University Northwest; Estados Unidos
Fil: Gerken, Ewan. University of Adelaide; Australia
Fil: Hindges, Robert. Kings College London (kcl);
Fil: Kenney, Justin. Wayne State University (wayne State University); Estados Unidos
Fil: Kleshchev, Maxim. Universidade Federal de Santa Maria; Brasil
Fil: Kolesnikov, Tatiana. Sirius University of Science and Technology; Rusia
Fil: Leggieri, Adele. Queen Mary University of London; Reino Unido
Fil: Khatsko, Sergey. Ural Federal University; Rusia
Fil: Lardelli, Michael. University of Adelaide; Australia
Fil: Lodetti, Guilherme. University of Southern Santa Catarina; Brasil
Fil: Lombardelli, Giulia. New York University; Estados Unidos
Fil: Medan, Violeta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Machado Moutinho, Lirane. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Materia
Anxiety
Data reproducibility
Experimental standardization
Sex differences
Zebrafish
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/258303

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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory StudyParker, MatthewHillman, CourtneyFontana, BarbaraAmstislavskaya, TamaraGorbunova, MariaAltenhofen, StefaniBarthelson, KarissaBastos, LeonardoBorba, JoãoBonan, CarlaBrennan, CarolineFarias Cea, AmauryCooper, AustinCorcoran, JamieDondossola, EduardoMartinez Duran, LuisGallas Lopes, MatheusGalstyan, DavidGarcia, EllaGerken, EwanHindges, RobertKenney, JustinKleshchev, MaximKolesnikov, TatianaLeggieri, AdeleKhatsko, SergeyLardelli, MichaelLodetti, GuilhermeLombardelli, GiuliaMedan, VioletaMachado Moutinho, LiraneAnxietyData reproducibilityExperimental standardizationSex differencesZebrafishhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The reproducibility crisis in bioscience, characterized by inconsistent study results, impedes our understanding of biological processes and global collaborative studies offer a unique solution. This study is the first global collaboration using the zebrafish (Danio rerio) novel tank test, a behavioral assay for anxiety-like responses. We analyzed data from 20 laboratories worldwide, focusing on housing conditions and experimental setups. Our study included 488 adult zebrafish, tested for 5 min, focusing on a variety of variables. Key findings show females exhibit more anxiety-like behavior than males, underscoring sex as a critical variable. Housing conditions, including higher stocking densities and specific feed types, influenced anxiety levels. Optimal conditions (5 fish/L) and nutritionally rich feeds (e.g., rotifers), mitigated anxiety-like behaviors. Environmental stressors, like noise and transportation, significantly impacted behavior. We recommend standardizing protocols to account for sex differences, optimal stocking densities, nutritionally rich feeds, and minimizing stressors to improve zebrafish behavioral study reliability.Fil: Parker, Matthew. University of Surrey; Reino UnidoFil: Hillman, Courtney. University Of Surrey. Faculty Of Health And Medical Sciences.; Reino UnidoFil: Fontana, Barbara. Universidade Federal de Santa Maria; BrasilFil: Amstislavskaya, Tamara. Novosibirsk State University; RusiaFil: Gorbunova, Maria. Ural Federal University; RusiaFil: Altenhofen, Stefani. Pontificia Universidade Católica do Rio Grande do Sul; BrasilFil: Barthelson, Karissa. University of Adelaide; AustraliaFil: Bastos, Leonardo. Universidade Federal do Rio Grande do Sul; BrasilFil: Borba, João. Universidade Federal de Santa Maria; BrasilFil: Bonan, Carla. Pontificia Universidade Católica do Rio Grande do Sul; BrasilFil: Brennan, Caroline. No especifíca;Fil: Farias Cea, Amaury. Universidad de La Frontera; ChileFil: Cooper, Austin. University of Surrey; Reino UnidoFil: Corcoran, Jamie. University of Nebraska; Estados UnidosFil: Dondossola, Eduardo. University of Southern Santa Catarina; BrasilFil: Martinez Duran, Luis. Universidad de La Frontera; ChileFil: Gallas Lopes, Matheus. No especifíca;Fil: Galstyan, David. St. Petersburg State University; RusiaFil: Garcia, Ella. Indiana University Northwest; Estados UnidosFil: Gerken, Ewan. University of Adelaide; AustraliaFil: Hindges, Robert. Kings College London (kcl);Fil: Kenney, Justin. Wayne State University (wayne State University); Estados UnidosFil: Kleshchev, Maxim. Universidade Federal de Santa Maria; BrasilFil: Kolesnikov, Tatiana. Sirius University of Science and Technology; RusiaFil: Leggieri, Adele. Queen Mary University of London; Reino UnidoFil: Khatsko, Sergey. Ural Federal University; RusiaFil: Lardelli, Michael. University of Adelaide; AustraliaFil: Lodetti, Guilherme. University of Southern Santa Catarina; BrasilFil: Lombardelli, Giulia. New York University; Estados UnidosFil: Medan, Violeta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Machado Moutinho, Lirane. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaResearch Square Company2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/258303Parker, Matthew; Hillman, Courtney; Fontana, Barbara; Amstislavskaya, Tamara; Gorbunova, Maria; et al.; Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study; Research Square Company; Research Square; 10-2024; 1-372693-5015CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.21203/rs.3.rs-4849877/v1info:eu-repo/semantics/altIdentifier/url/https://www.researchsquare.com/article/rs-4849877/v1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:37Zoai:ri.conicet.gov.ar:11336/258303instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:38.273CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study
title Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study
spellingShingle Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study
Parker, Matthew
Anxiety
Data reproducibility
Experimental standardization
Sex differences
Zebrafish
title_short Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study
title_full Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study
title_fullStr Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study
title_full_unstemmed Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study
title_sort Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study
dc.creator.none.fl_str_mv Parker, Matthew
Hillman, Courtney
Fontana, Barbara
Amstislavskaya, Tamara
Gorbunova, Maria
Altenhofen, Stefani
Barthelson, Karissa
Bastos, Leonardo
Borba, João
Bonan, Carla
Brennan, Caroline
Farias Cea, Amaury
Cooper, Austin
Corcoran, Jamie
Dondossola, Eduardo
Martinez Duran, Luis
Gallas Lopes, Matheus
Galstyan, David
Garcia, Ella
Gerken, Ewan
Hindges, Robert
Kenney, Justin
Kleshchev, Maxim
Kolesnikov, Tatiana
Leggieri, Adele
Khatsko, Sergey
Lardelli, Michael
Lodetti, Guilherme
Lombardelli, Giulia
Medan, Violeta
Machado Moutinho, Lirane
author Parker, Matthew
author_facet Parker, Matthew
Hillman, Courtney
Fontana, Barbara
Amstislavskaya, Tamara
Gorbunova, Maria
Altenhofen, Stefani
Barthelson, Karissa
Bastos, Leonardo
Borba, João
Bonan, Carla
Brennan, Caroline
Farias Cea, Amaury
Cooper, Austin
Corcoran, Jamie
Dondossola, Eduardo
Martinez Duran, Luis
Gallas Lopes, Matheus
Galstyan, David
Garcia, Ella
Gerken, Ewan
Hindges, Robert
Kenney, Justin
Kleshchev, Maxim
Kolesnikov, Tatiana
Leggieri, Adele
Khatsko, Sergey
Lardelli, Michael
Lodetti, Guilherme
Lombardelli, Giulia
Medan, Violeta
Machado Moutinho, Lirane
author_role author
author2 Hillman, Courtney
Fontana, Barbara
Amstislavskaya, Tamara
Gorbunova, Maria
Altenhofen, Stefani
Barthelson, Karissa
Bastos, Leonardo
Borba, João
Bonan, Carla
Brennan, Caroline
Farias Cea, Amaury
Cooper, Austin
Corcoran, Jamie
Dondossola, Eduardo
Martinez Duran, Luis
Gallas Lopes, Matheus
Galstyan, David
Garcia, Ella
Gerken, Ewan
Hindges, Robert
Kenney, Justin
Kleshchev, Maxim
Kolesnikov, Tatiana
Leggieri, Adele
Khatsko, Sergey
Lardelli, Michael
Lodetti, Guilherme
Lombardelli, Giulia
Medan, Violeta
Machado Moutinho, Lirane
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Anxiety
Data reproducibility
Experimental standardization
Sex differences
Zebrafish
topic Anxiety
Data reproducibility
Experimental standardization
Sex differences
Zebrafish
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The reproducibility crisis in bioscience, characterized by inconsistent study results, impedes our understanding of biological processes and global collaborative studies offer a unique solution. This study is the first global collaboration using the zebrafish (Danio rerio) novel tank test, a behavioral assay for anxiety-like responses. We analyzed data from 20 laboratories worldwide, focusing on housing conditions and experimental setups. Our study included 488 adult zebrafish, tested for 5 min, focusing on a variety of variables. Key findings show females exhibit more anxiety-like behavior than males, underscoring sex as a critical variable. Housing conditions, including higher stocking densities and specific feed types, influenced anxiety levels. Optimal conditions (5 fish/L) and nutritionally rich feeds (e.g., rotifers), mitigated anxiety-like behaviors. Environmental stressors, like noise and transportation, significantly impacted behavior. We recommend standardizing protocols to account for sex differences, optimal stocking densities, nutritionally rich feeds, and minimizing stressors to improve zebrafish behavioral study reliability.
Fil: Parker, Matthew. University of Surrey; Reino Unido
Fil: Hillman, Courtney. University Of Surrey. Faculty Of Health And Medical Sciences.; Reino Unido
Fil: Fontana, Barbara. Universidade Federal de Santa Maria; Brasil
Fil: Amstislavskaya, Tamara. Novosibirsk State University; Rusia
Fil: Gorbunova, Maria. Ural Federal University; Rusia
Fil: Altenhofen, Stefani. Pontificia Universidade Católica do Rio Grande do Sul; Brasil
Fil: Barthelson, Karissa. University of Adelaide; Australia
Fil: Bastos, Leonardo. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Borba, João. Universidade Federal de Santa Maria; Brasil
Fil: Bonan, Carla. Pontificia Universidade Católica do Rio Grande do Sul; Brasil
Fil: Brennan, Caroline. No especifíca;
Fil: Farias Cea, Amaury. Universidad de La Frontera; Chile
Fil: Cooper, Austin. University of Surrey; Reino Unido
Fil: Corcoran, Jamie. University of Nebraska; Estados Unidos
Fil: Dondossola, Eduardo. University of Southern Santa Catarina; Brasil
Fil: Martinez Duran, Luis. Universidad de La Frontera; Chile
Fil: Gallas Lopes, Matheus. No especifíca;
Fil: Galstyan, David. St. Petersburg State University; Rusia
Fil: Garcia, Ella. Indiana University Northwest; Estados Unidos
Fil: Gerken, Ewan. University of Adelaide; Australia
Fil: Hindges, Robert. Kings College London (kcl);
Fil: Kenney, Justin. Wayne State University (wayne State University); Estados Unidos
Fil: Kleshchev, Maxim. Universidade Federal de Santa Maria; Brasil
Fil: Kolesnikov, Tatiana. Sirius University of Science and Technology; Rusia
Fil: Leggieri, Adele. Queen Mary University of London; Reino Unido
Fil: Khatsko, Sergey. Ural Federal University; Rusia
Fil: Lardelli, Michael. University of Adelaide; Australia
Fil: Lodetti, Guilherme. University of Southern Santa Catarina; Brasil
Fil: Lombardelli, Giulia. New York University; Estados Unidos
Fil: Medan, Violeta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Machado Moutinho, Lirane. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
description The reproducibility crisis in bioscience, characterized by inconsistent study results, impedes our understanding of biological processes and global collaborative studies offer a unique solution. This study is the first global collaboration using the zebrafish (Danio rerio) novel tank test, a behavioral assay for anxiety-like responses. We analyzed data from 20 laboratories worldwide, focusing on housing conditions and experimental setups. Our study included 488 adult zebrafish, tested for 5 min, focusing on a variety of variables. Key findings show females exhibit more anxiety-like behavior than males, underscoring sex as a critical variable. Housing conditions, including higher stocking densities and specific feed types, influenced anxiety levels. Optimal conditions (5 fish/L) and nutritionally rich feeds (e.g., rotifers), mitigated anxiety-like behaviors. Environmental stressors, like noise and transportation, significantly impacted behavior. We recommend standardizing protocols to account for sex differences, optimal stocking densities, nutritionally rich feeds, and minimizing stressors to improve zebrafish behavioral study reliability.
publishDate 2024
dc.date.none.fl_str_mv 2024-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/258303
Parker, Matthew; Hillman, Courtney; Fontana, Barbara; Amstislavskaya, Tamara; Gorbunova, Maria; et al.; Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study; Research Square Company; Research Square; 10-2024; 1-37
2693-5015
CONICET Digital
CONICET
url http://hdl.handle.net/11336/258303
identifier_str_mv Parker, Matthew; Hillman, Courtney; Fontana, Barbara; Amstislavskaya, Tamara; Gorbunova, Maria; et al.; Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study; Research Square Company; Research Square; 10-2024; 1-37
2693-5015
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.21203/rs.3.rs-4849877/v1
info:eu-repo/semantics/altIdentifier/url/https://www.researchsquare.com/article/rs-4849877/v1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Research Square Company
publisher.none.fl_str_mv Research Square Company
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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