Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study

Autores: Parker, Matthew; Hillman, Courtney; Fontana, Barbara; Amstislavskaya, Tamara; Gorbunova, Maria; Altenhofen, Stefani; Barthelson, Karissa; Bastos, Leonardo; Borba, João; Bonan, Carla; Brennan, Caroline; Farias Cea, Amaury; Cooper, Austin; Corcoran, Jamie; Dondossola, Eduardo; Martinez Duran, Luis; Gallas Lopes, Matheus; Galstyan, David; Garcia, Ella; Gerken, Ewan; Hindges, Robert; Kenney, Justin; Kleshchev, Maxim; Kolesnikov, Tatiana; Leggieri, Adele; Khatsko, Sergey; Lardelli, Michael; Lodetti, Guilherme; Lombardelli, Giulia; Medan, Violeta; Machado Moutinho, Lirane
Año de publicación: 2024
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: The reproducibility crisis in bioscience, characterized by inconsistent study results, impedes our understanding of biological processes and global collaborative studies offer a unique solution. This study is the first global collaboration using the zebrafish (Danio rerio) novel tank test, a behavioral assay for anxiety-like responses. We analyzed data from 20 laboratories worldwide, focusing on housing conditions and experimental setups. Our study included 488 adult zebrafish, tested for 5 min, focusing on a variety of variables. Key findings show females exhibit more anxiety-like behavior than males, underscoring sex as a critical variable. Housing conditions, including higher stocking densities and specific feed types, influenced anxiety levels. Optimal conditions (5 fish/L) and nutritionally rich feeds (e.g., rotifers), mitigated anxiety-like behaviors. Environmental stressors, like noise and transportation, significantly impacted behavior. We recommend standardizing protocols to account for sex differences, optimal stocking densities, nutritionally rich feeds, and minimizing stressors to improve zebrafish behavioral study reliability.
Fil: Parker, Matthew. University of Surrey; Reino Unido
Fil: Hillman, Courtney. University Of Surrey. Faculty Of Health And Medical Sciences.; Reino Unido
Fil: Fontana, Barbara. Universidade Federal de Santa Maria; Brasil
Fil: Amstislavskaya, Tamara. Novosibirsk State University; Rusia
Fil: Gorbunova, Maria. Ural Federal University; Rusia
Fil: Altenhofen, Stefani. Pontificia Universidade Católica do Rio Grande do Sul; Brasil
Fil: Barthelson, Karissa. University of Adelaide; Australia
Fil: Bastos, Leonardo. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Borba, João. Universidade Federal de Santa Maria; Brasil
Fil: Bonan, Carla. Pontificia Universidade Católica do Rio Grande do Sul; Brasil
Fil: Brennan, Caroline. No especifíca;
Fil: Farias Cea, Amaury. Universidad de La Frontera; Chile
Fil: Cooper, Austin. University of Surrey; Reino Unido
Fil: Corcoran, Jamie. University of Nebraska; Estados Unidos
Fil: Dondossola, Eduardo. University of Southern Santa Catarina; Brasil
Fil: Martinez Duran, Luis. Universidad de La Frontera; Chile
Fil: Gallas Lopes, Matheus. No especifíca;
Fil: Galstyan, David. St. Petersburg State University; Rusia
Fil: Garcia, Ella. Indiana University Northwest; Estados Unidos
Fil: Gerken, Ewan. University of Adelaide; Australia
Fil: Hindges, Robert. Kings College London (kcl);
Fil: Kenney, Justin. Wayne State University (wayne State University); Estados Unidos
Fil: Kleshchev, Maxim. Universidade Federal de Santa Maria; Brasil
Fil: Kolesnikov, Tatiana. Sirius University of Science and Technology; Rusia
Fil: Leggieri, Adele. Queen Mary University of London; Reino Unido
Fil: Khatsko, Sergey. Ural Federal University; Rusia
Fil: Lardelli, Michael. University of Adelaide; Australia
Fil: Lodetti, Guilherme. University of Southern Santa Catarina; Brasil
Fil: Lombardelli, Giulia. New York University; Estados Unidos
Fil: Medan, Violeta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Machado Moutinho, Lirane. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Materia: Anxiety
Data reproducibility
Experimental standardization
Sex differences
Zebrafish
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/258303

Acceder

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repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory StudyParker, MatthewHillman, CourtneyFontana, BarbaraAmstislavskaya, TamaraGorbunova, MariaAltenhofen, StefaniBarthelson, KarissaBastos, LeonardoBorba, JoãoBonan, CarlaBrennan, CarolineFarias Cea, AmauryCooper, AustinCorcoran, JamieDondossola, EduardoMartinez Duran, LuisGallas Lopes, MatheusGalstyan, DavidGarcia, EllaGerken, EwanHindges, RobertKenney, JustinKleshchev, MaximKolesnikov, TatianaLeggieri, AdeleKhatsko, SergeyLardelli, MichaelLodetti, GuilhermeLombardelli, GiuliaMedan, VioletaMachado Moutinho, LiraneAnxietyData reproducibilityExperimental standardizationSex differencesZebrafishhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The reproducibility crisis in bioscience, characterized by inconsistent study results, impedes our understanding of biological processes and global collaborative studies offer a unique solution. This study is the first global collaboration using the zebrafish (Danio rerio) novel tank test, a behavioral assay for anxiety-like responses. We analyzed data from 20 laboratories worldwide, focusing on housing conditions and experimental setups. Our study included 488 adult zebrafish, tested for 5 min, focusing on a variety of variables. Key findings show females exhibit more anxiety-like behavior than males, underscoring sex as a critical variable. Housing conditions, including higher stocking densities and specific feed types, influenced anxiety levels. Optimal conditions (5 fish/L) and nutritionally rich feeds (e.g., rotifers), mitigated anxiety-like behaviors. Environmental stressors, like noise and transportation, significantly impacted behavior. We recommend standardizing protocols to account for sex differences, optimal stocking densities, nutritionally rich feeds, and minimizing stressors to improve zebrafish behavioral study reliability.Fil: Parker, Matthew. University of Surrey; Reino UnidoFil: Hillman, Courtney. University Of Surrey. Faculty Of Health And Medical Sciences.; Reino UnidoFil: Fontana, Barbara. Universidade Federal de Santa Maria; BrasilFil: Amstislavskaya, Tamara. Novosibirsk State University; RusiaFil: Gorbunova, Maria. Ural Federal University; RusiaFil: Altenhofen, Stefani. Pontificia Universidade Católica do Rio Grande do Sul; BrasilFil: Barthelson, Karissa. University of Adelaide; AustraliaFil: Bastos, Leonardo. Universidade Federal do Rio Grande do Sul; BrasilFil: Borba, João. Universidade Federal de Santa Maria; BrasilFil: Bonan, Carla. Pontificia Universidade Católica do Rio Grande do Sul; BrasilFil: Brennan, Caroline. No especifíca;Fil: Farias Cea, Amaury. Universidad de La Frontera; ChileFil: Cooper, Austin. University of Surrey; Reino UnidoFil: Corcoran, Jamie. University of Nebraska; Estados UnidosFil: Dondossola, Eduardo. University of Southern Santa Catarina; BrasilFil: Martinez Duran, Luis. Universidad de La Frontera; ChileFil: Gallas Lopes, Matheus. No especifíca;Fil: Galstyan, David. St. Petersburg State University; RusiaFil: Garcia, Ella. Indiana University Northwest; Estados UnidosFil: Gerken, Ewan. University of Adelaide; AustraliaFil: Hindges, Robert. Kings College London (kcl);Fil: Kenney, Justin. Wayne State University (wayne State University); Estados UnidosFil: Kleshchev, Maxim. Universidade Federal de Santa Maria; BrasilFil: Kolesnikov, Tatiana. Sirius University of Science and Technology; RusiaFil: Leggieri, Adele. Queen Mary University of London; Reino UnidoFil: Khatsko, Sergey. Ural Federal University; RusiaFil: Lardelli, Michael. University of Adelaide; AustraliaFil: Lodetti, Guilherme. University of Southern Santa Catarina; BrasilFil: Lombardelli, Giulia. New York University; Estados UnidosFil: Medan, Violeta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Machado Moutinho, Lirane. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaResearch Square Company2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/258303Parker, Matthew; Hillman, Courtney; Fontana, Barbara; Amstislavskaya, Tamara; Gorbunova, Maria; et al.; Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study; Research Square Company; Research Square; 10-2024; 1-372693-5015CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.21203/rs.3.rs-4849877/v1info:eu-repo/semantics/altIdentifier/url/https://www.researchsquare.com/article/rs-4849877/v1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:22:02Zoai:ri.conicet.gov.ar:11336/258303instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:22:03.245CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study
title	Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study
spellingShingle	Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study Parker, Matthew Anxiety Data reproducibility Experimental standardization Sex differences Zebrafish
title_short	Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study
title_full	Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study
title_fullStr	Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study
title_full_unstemmed	Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study
title_sort	Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study
dc.creator.none.fl_str_mv	Parker, Matthew Hillman, Courtney Fontana, Barbara Amstislavskaya, Tamara Gorbunova, Maria Altenhofen, Stefani Barthelson, Karissa Bastos, Leonardo Borba, João Bonan, Carla Brennan, Caroline Farias Cea, Amaury Cooper, Austin Corcoran, Jamie Dondossola, Eduardo Martinez Duran, Luis Gallas Lopes, Matheus Galstyan, David Garcia, Ella Gerken, Ewan Hindges, Robert Kenney, Justin Kleshchev, Maxim Kolesnikov, Tatiana Leggieri, Adele Khatsko, Sergey Lardelli, Michael Lodetti, Guilherme Lombardelli, Giulia Medan, Violeta Machado Moutinho, Lirane
author	Parker, Matthew
author_facet	Parker, Matthew Hillman, Courtney Fontana, Barbara Amstislavskaya, Tamara Gorbunova, Maria Altenhofen, Stefani Barthelson, Karissa Bastos, Leonardo Borba, João Bonan, Carla Brennan, Caroline Farias Cea, Amaury Cooper, Austin Corcoran, Jamie Dondossola, Eduardo Martinez Duran, Luis Gallas Lopes, Matheus Galstyan, David Garcia, Ella Gerken, Ewan Hindges, Robert Kenney, Justin Kleshchev, Maxim Kolesnikov, Tatiana Leggieri, Adele Khatsko, Sergey Lardelli, Michael Lodetti, Guilherme Lombardelli, Giulia Medan, Violeta Machado Moutinho, Lirane
author_role	author
author2	Hillman, Courtney Fontana, Barbara Amstislavskaya, Tamara Gorbunova, Maria Altenhofen, Stefani Barthelson, Karissa Bastos, Leonardo Borba, João Bonan, Carla Brennan, Caroline Farias Cea, Amaury Cooper, Austin Corcoran, Jamie Dondossola, Eduardo Martinez Duran, Luis Gallas Lopes, Matheus Galstyan, David Garcia, Ella Gerken, Ewan Hindges, Robert Kenney, Justin Kleshchev, Maxim Kolesnikov, Tatiana Leggieri, Adele Khatsko, Sergey Lardelli, Michael Lodetti, Guilherme Lombardelli, Giulia Medan, Violeta Machado Moutinho, Lirane
author2_role	author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Anxiety Data reproducibility Experimental standardization Sex differences Zebrafish
topic	Anxiety Data reproducibility Experimental standardization Sex differences Zebrafish
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	The reproducibility crisis in bioscience, characterized by inconsistent study results, impedes our understanding of biological processes and global collaborative studies offer a unique solution. This study is the first global collaboration using the zebrafish (Danio rerio) novel tank test, a behavioral assay for anxiety-like responses. We analyzed data from 20 laboratories worldwide, focusing on housing conditions and experimental setups. Our study included 488 adult zebrafish, tested for 5 min, focusing on a variety of variables. Key findings show females exhibit more anxiety-like behavior than males, underscoring sex as a critical variable. Housing conditions, including higher stocking densities and specific feed types, influenced anxiety levels. Optimal conditions (5 fish/L) and nutritionally rich feeds (e.g., rotifers), mitigated anxiety-like behaviors. Environmental stressors, like noise and transportation, significantly impacted behavior. We recommend standardizing protocols to account for sex differences, optimal stocking densities, nutritionally rich feeds, and minimizing stressors to improve zebrafish behavioral study reliability. Fil: Parker, Matthew. University of Surrey; Reino Unido Fil: Hillman, Courtney. University Of Surrey. Faculty Of Health And Medical Sciences.; Reino Unido Fil: Fontana, Barbara. Universidade Federal de Santa Maria; Brasil Fil: Amstislavskaya, Tamara. Novosibirsk State University; Rusia Fil: Gorbunova, Maria. Ural Federal University; Rusia Fil: Altenhofen, Stefani. Pontificia Universidade Católica do Rio Grande do Sul; Brasil Fil: Barthelson, Karissa. University of Adelaide; Australia Fil: Bastos, Leonardo. Universidade Federal do Rio Grande do Sul; Brasil Fil: Borba, João. Universidade Federal de Santa Maria; Brasil Fil: Bonan, Carla. Pontificia Universidade Católica do Rio Grande do Sul; Brasil Fil: Brennan, Caroline. No especifíca; Fil: Farias Cea, Amaury. Universidad de La Frontera; Chile Fil: Cooper, Austin. University of Surrey; Reino Unido Fil: Corcoran, Jamie. University of Nebraska; Estados Unidos Fil: Dondossola, Eduardo. University of Southern Santa Catarina; Brasil Fil: Martinez Duran, Luis. Universidad de La Frontera; Chile Fil: Gallas Lopes, Matheus. No especifíca; Fil: Galstyan, David. St. Petersburg State University; Rusia Fil: Garcia, Ella. Indiana University Northwest; Estados Unidos Fil: Gerken, Ewan. University of Adelaide; Australia Fil: Hindges, Robert. Kings College London (kcl); Fil: Kenney, Justin. Wayne State University (wayne State University); Estados Unidos Fil: Kleshchev, Maxim. Universidade Federal de Santa Maria; Brasil Fil: Kolesnikov, Tatiana. Sirius University of Science and Technology; Rusia Fil: Leggieri, Adele. Queen Mary University of London; Reino Unido Fil: Khatsko, Sergey. Ural Federal University; Rusia Fil: Lardelli, Michael. University of Adelaide; Australia Fil: Lodetti, Guilherme. University of Southern Santa Catarina; Brasil Fil: Lombardelli, Giulia. New York University; Estados Unidos Fil: Medan, Violeta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Machado Moutinho, Lirane. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
description	The reproducibility crisis in bioscience, characterized by inconsistent study results, impedes our understanding of biological processes and global collaborative studies offer a unique solution. This study is the first global collaboration using the zebrafish (Danio rerio) novel tank test, a behavioral assay for anxiety-like responses. We analyzed data from 20 laboratories worldwide, focusing on housing conditions and experimental setups. Our study included 488 adult zebrafish, tested for 5 min, focusing on a variety of variables. Key findings show females exhibit more anxiety-like behavior than males, underscoring sex as a critical variable. Housing conditions, including higher stocking densities and specific feed types, influenced anxiety levels. Optimal conditions (5 fish/L) and nutritionally rich feeds (e.g., rotifers), mitigated anxiety-like behaviors. Environmental stressors, like noise and transportation, significantly impacted behavior. We recommend standardizing protocols to account for sex differences, optimal stocking densities, nutritionally rich feeds, and minimizing stressors to improve zebrafish behavioral study reliability.
publishDate	2024
dc.date.none.fl_str_mv	2024-10
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/258303 Parker, Matthew; Hillman, Courtney; Fontana, Barbara; Amstislavskaya, Tamara; Gorbunova, Maria; et al.; Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study; Research Square Company; Research Square; 10-2024; 1-37 2693-5015 CONICET Digital CONICET
url	http://hdl.handle.net/11336/258303
identifier_str_mv	Parker, Matthew; Hillman, Courtney; Fontana, Barbara; Amstislavskaya, Tamara; Gorbunova, Maria; et al.; Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study; Research Square Company; Research Square; 10-2024; 1-37 2693-5015 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.21203/rs.3.rs-4849877/v1 info:eu-repo/semantics/altIdentifier/url/https://www.researchsquare.com/article/rs-4849877/v1
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rights_invalid_str_mv	https://creativecommons.org/licenses/by/2.5/ar/
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dc.publisher.none.fl_str_mv	Research Square Company
publisher.none.fl_str_mv	Research Square Company
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
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instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score	12.982451

Housing and Husbandry Factors Affecting Zebrafish (Danio rerio) Novel Tank Test Responses: A Global Multi-Laboratory Study

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