Non-covalent functionalization of multi-walled carbon nanotubes with cytochrome c: Enhanced direct electron transfer and analytical applications

Autores
Eguilaz Rubio, Marcos; Gutierrez, Alejandro; Rivas, Gustavo Adolfo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work reports the non-covalent functionalization (dispersion) of multi-walled carbon nanotubes (MWCNTs) with cytochrome c (Cyt c), the direct electron transfer (DET) after drop-coating deposition of MWCNTs-Cyt c dispersion on glassy carbon electrodes (GCE), and the analytical applications for the highly sensitive quantification of hydrogen peroxide. The dispersion and the resulting modified electrodes were studied by UV-visible spectroscopy, scanning electron microscopy, and electrochemical techniques. The drastic treatment for dispersing the MWCNTs (5.0 min sonication in water with ultrasonic tip) produces a partial denaturation that facilitates the interaction of Cyt c with the CNTs and makes possible an efficient electron transfer between the heme group and the electrode. A critical analysis of the influence of different experimental conditions on the efficiency of the dispersion and on the performance of GCE modified with MWCNTs-Cyt c dispersion is also reported. The analytical parameters obtained with GCE/MWCNTs-Cyt c for the amperometric quantification of hydrogen peroxide at -0.100 V were: sensitivity of (43 ± 1) mA M-1 cm-2, linear range between 1.0 × 10-6 and 1.6 × 10-4 M, detection limit of 1.5 × 10-7 M, reproducibility of 3.1% and repeatability of 3.4%. The biosensor was successfully used for the quantification of hydrogen peroxide in mouthwash and spiked milk samples.
Fil: Eguilaz Rubio, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Gutierrez, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
Cnt Functionalization
Cytochrome C
Direct Electron Transfer
Electrochemical Biosensor
Hydrogen Peroxide
Multi-Walled Carbon Nanotubes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/52601
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/52601
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Non-covalent functionalization of multi-walled carbon nanotubes with cytochrome c: Enhanced direct electron transfer and analytical applicationsEguilaz Rubio, MarcosGutierrez, AlejandroRivas, Gustavo AdolfoCnt FunctionalizationCytochrome CDirect Electron TransferElectrochemical BiosensorHydrogen PeroxideMulti-Walled Carbon Nanotubeshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This work reports the non-covalent functionalization (dispersion) of multi-walled carbon nanotubes (MWCNTs) with cytochrome c (Cyt c), the direct electron transfer (DET) after drop-coating deposition of MWCNTs-Cyt c dispersion on glassy carbon electrodes (GCE), and the analytical applications for the highly sensitive quantification of hydrogen peroxide. The dispersion and the resulting modified electrodes were studied by UV-visible spectroscopy, scanning electron microscopy, and electrochemical techniques. The drastic treatment for dispersing the MWCNTs (5.0 min sonication in water with ultrasonic tip) produces a partial denaturation that facilitates the interaction of Cyt c with the CNTs and makes possible an efficient electron transfer between the heme group and the electrode. A critical analysis of the influence of different experimental conditions on the efficiency of the dispersion and on the performance of GCE modified with MWCNTs-Cyt c dispersion is also reported. The analytical parameters obtained with GCE/MWCNTs-Cyt c for the amperometric quantification of hydrogen peroxide at -0.100 V were: sensitivity of (43 ± 1) mA M-1 cm-2, linear range between 1.0 × 10-6 and 1.6 × 10-4 M, detection limit of 1.5 × 10-7 M, reproducibility of 3.1% and repeatability of 3.4%. The biosensor was successfully used for the quantification of hydrogen peroxide in mouthwash and spiked milk samples.Fil: Eguilaz Rubio, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Gutierrez, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaElsevier Science Sa2016-03-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/52601Eguilaz Rubio, Marcos; Gutierrez, Alejandro; Rivas, Gustavo Adolfo; Non-covalent functionalization of multi-walled carbon nanotubes with cytochrome c: Enhanced direct electron transfer and analytical applications; Elsevier Science Sa; Sensors and Actuators B: Chemical; 225; 31-3-2016; 74-800925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400515305931info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2015.11.011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T11:40:23Zoai:ri.conicet.gov.ar:11336/52601instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 11:40:23.999CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Non-covalent functionalization of multi-walled carbon nanotubes with cytochrome c: Enhanced direct electron transfer and analytical applications
title Non-covalent functionalization of multi-walled carbon nanotubes with cytochrome c: Enhanced direct electron transfer and analytical applications
spellingShingle Non-covalent functionalization of multi-walled carbon nanotubes with cytochrome c: Enhanced direct electron transfer and analytical applications
Eguilaz Rubio, Marcos
Cnt Functionalization
Cytochrome C
Direct Electron Transfer
Electrochemical Biosensor
Hydrogen Peroxide
Multi-Walled Carbon Nanotubes
title_short Non-covalent functionalization of multi-walled carbon nanotubes with cytochrome c: Enhanced direct electron transfer and analytical applications
title_full Non-covalent functionalization of multi-walled carbon nanotubes with cytochrome c: Enhanced direct electron transfer and analytical applications
title_fullStr Non-covalent functionalization of multi-walled carbon nanotubes with cytochrome c: Enhanced direct electron transfer and analytical applications
title_full_unstemmed Non-covalent functionalization of multi-walled carbon nanotubes with cytochrome c: Enhanced direct electron transfer and analytical applications
title_sort Non-covalent functionalization of multi-walled carbon nanotubes with cytochrome c: Enhanced direct electron transfer and analytical applications
dc.creator.none.fl_str_mv Eguilaz Rubio, Marcos
Gutierrez, Alejandro
Rivas, Gustavo Adolfo
author Eguilaz Rubio, Marcos
author_facet Eguilaz Rubio, Marcos
Gutierrez, Alejandro
Rivas, Gustavo Adolfo
author_role author
author2 Gutierrez, Alejandro
Rivas, Gustavo Adolfo
author2_role author
author
dc.subject.none.fl_str_mv Cnt Functionalization
Cytochrome C
Direct Electron Transfer
Electrochemical Biosensor
Hydrogen Peroxide
Multi-Walled Carbon Nanotubes
topic Cnt Functionalization
Cytochrome C
Direct Electron Transfer
Electrochemical Biosensor
Hydrogen Peroxide
Multi-Walled Carbon Nanotubes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This work reports the non-covalent functionalization (dispersion) of multi-walled carbon nanotubes (MWCNTs) with cytochrome c (Cyt c), the direct electron transfer (DET) after drop-coating deposition of MWCNTs-Cyt c dispersion on glassy carbon electrodes (GCE), and the analytical applications for the highly sensitive quantification of hydrogen peroxide. The dispersion and the resulting modified electrodes were studied by UV-visible spectroscopy, scanning electron microscopy, and electrochemical techniques. The drastic treatment for dispersing the MWCNTs (5.0 min sonication in water with ultrasonic tip) produces a partial denaturation that facilitates the interaction of Cyt c with the CNTs and makes possible an efficient electron transfer between the heme group and the electrode. A critical analysis of the influence of different experimental conditions on the efficiency of the dispersion and on the performance of GCE modified with MWCNTs-Cyt c dispersion is also reported. The analytical parameters obtained with GCE/MWCNTs-Cyt c for the amperometric quantification of hydrogen peroxide at -0.100 V were: sensitivity of (43 ± 1) mA M-1 cm-2, linear range between 1.0 × 10-6 and 1.6 × 10-4 M, detection limit of 1.5 × 10-7 M, reproducibility of 3.1% and repeatability of 3.4%. The biosensor was successfully used for the quantification of hydrogen peroxide in mouthwash and spiked milk samples.
Fil: Eguilaz Rubio, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Gutierrez, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description This work reports the non-covalent functionalization (dispersion) of multi-walled carbon nanotubes (MWCNTs) with cytochrome c (Cyt c), the direct electron transfer (DET) after drop-coating deposition of MWCNTs-Cyt c dispersion on glassy carbon electrodes (GCE), and the analytical applications for the highly sensitive quantification of hydrogen peroxide. The dispersion and the resulting modified electrodes were studied by UV-visible spectroscopy, scanning electron microscopy, and electrochemical techniques. The drastic treatment for dispersing the MWCNTs (5.0 min sonication in water with ultrasonic tip) produces a partial denaturation that facilitates the interaction of Cyt c with the CNTs and makes possible an efficient electron transfer between the heme group and the electrode. A critical analysis of the influence of different experimental conditions on the efficiency of the dispersion and on the performance of GCE modified with MWCNTs-Cyt c dispersion is also reported. The analytical parameters obtained with GCE/MWCNTs-Cyt c for the amperometric quantification of hydrogen peroxide at -0.100 V were: sensitivity of (43 ± 1) mA M-1 cm-2, linear range between 1.0 × 10-6 and 1.6 × 10-4 M, detection limit of 1.5 × 10-7 M, reproducibility of 3.1% and repeatability of 3.4%. The biosensor was successfully used for the quantification of hydrogen peroxide in mouthwash and spiked milk samples.
publishDate 2016
dc.date.none.fl_str_mv 2016-03-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/52601
Eguilaz Rubio, Marcos; Gutierrez, Alejandro; Rivas, Gustavo Adolfo; Non-covalent functionalization of multi-walled carbon nanotubes with cytochrome c: Enhanced direct electron transfer and analytical applications; Elsevier Science Sa; Sensors and Actuators B: Chemical; 225; 31-3-2016; 74-80
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/52601
identifier_str_mv Eguilaz Rubio, Marcos; Gutierrez, Alejandro; Rivas, Gustavo Adolfo; Non-covalent functionalization of multi-walled carbon nanotubes with cytochrome c: Enhanced direct electron transfer and analytical applications; Elsevier Science Sa; Sensors and Actuators B: Chemical; 225; 31-3-2016; 74-80
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400515305931
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2015.11.011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.10058

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