Development of an amperometric biosensor based on peroxidases to quantify citrinin in rice samples

Autores
Zachetti, Vanessa Gimena Lourdes; Granero, Adrian Marcelo; Robledo, Sebastian Noel; Zon, María Alicia; Fernandez, Hector
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An amperometric biosensor based on horseradish peroxidase (EC1.11.1.7,H2O2-oxide-reductases) to determine the content of citrinin mycotoxin in rice samples is proposed by the first time. The method uses carbon paste electrodes filled up with multi-walled carbon nanotubes embedded in a mineral oil, horseradish peroxidase, and ferrocene as a redox mediator. The biosensor is covered externally with a dialysis membrane, which is fixed to the body side of the electrode with a Teflon laboratory film, and an O-ring. The reproducibility and the repeatability were of 7.0% and 3.0%, respectively, showing a very good biosensor performance. The calibration curve was linear in a concentration range from 1 to 11.6 nM. The limits of detection and quantification were 0.25 nM and 0.75 nM, respectively. For comparison, the citrinin content in rice samples was also determined by fluorimetric measurements. A very good correlation was obtained between the electrochemical and spectrophotometric methods.
Fil: Zachetti, Vanessa Gimena Lourdes. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Granero, Adrian Marcelo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Robledo, Sebastian Noel. Universidad Nacional de Rio Cuarto. Facultad de Ingeniería. Departamento de Tecnología Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Hector. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Electrochemical Biosensor
Citrinin
Multi-Walled Carbon Nanotubes
Peroxidase Enzymes
Mycotoxins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22650
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/22650
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of an amperometric biosensor based on peroxidases to quantify citrinin in rice samplesZachetti, Vanessa Gimena LourdesGranero, Adrian MarceloRobledo, Sebastian NoelZon, María AliciaFernandez, HectorElectrochemical BiosensorCitrininMulti-Walled Carbon NanotubesPeroxidase EnzymesMycotoxinshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1An amperometric biosensor based on horseradish peroxidase (EC1.11.1.7,H2O2-oxide-reductases) to determine the content of citrinin mycotoxin in rice samples is proposed by the first time. The method uses carbon paste electrodes filled up with multi-walled carbon nanotubes embedded in a mineral oil, horseradish peroxidase, and ferrocene as a redox mediator. The biosensor is covered externally with a dialysis membrane, which is fixed to the body side of the electrode with a Teflon laboratory film, and an O-ring. The reproducibility and the repeatability were of 7.0% and 3.0%, respectively, showing a very good biosensor performance. The calibration curve was linear in a concentration range from 1 to 11.6 nM. The limits of detection and quantification were 0.25 nM and 0.75 nM, respectively. For comparison, the citrinin content in rice samples was also determined by fluorimetric measurements. A very good correlation was obtained between the electrochemical and spectrophotometric methods.Fil: Zachetti, Vanessa Gimena Lourdes. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Granero, Adrian Marcelo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Robledo, Sebastian Noel. Universidad Nacional de Rio Cuarto. Facultad de Ingeniería. Departamento de Tecnología Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernandez, Hector. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22650Zachetti, Vanessa Gimena Lourdes; Granero, Adrian Marcelo; Robledo, Sebastian Noel; Zon, María Alicia; Fernandez, Hector; Development of an amperometric biosensor based on peroxidases to quantify citrinin in rice samples; Elsevier Science; Bioelectrochemistry; 91; 1-2013; 37-431567-5394CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bioelechem.2012.12.004info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1567539413000042info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:05:56Zoai:ri.conicet.gov.ar:11336/22650instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:05:57.021CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of an amperometric biosensor based on peroxidases to quantify citrinin in rice samples
title Development of an amperometric biosensor based on peroxidases to quantify citrinin in rice samples
spellingShingle Development of an amperometric biosensor based on peroxidases to quantify citrinin in rice samples
Zachetti, Vanessa Gimena Lourdes
Electrochemical Biosensor
Citrinin
Multi-Walled Carbon Nanotubes
Peroxidase Enzymes
Mycotoxins
title_short Development of an amperometric biosensor based on peroxidases to quantify citrinin in rice samples
title_full Development of an amperometric biosensor based on peroxidases to quantify citrinin in rice samples
title_fullStr Development of an amperometric biosensor based on peroxidases to quantify citrinin in rice samples
title_full_unstemmed Development of an amperometric biosensor based on peroxidases to quantify citrinin in rice samples
title_sort Development of an amperometric biosensor based on peroxidases to quantify citrinin in rice samples
dc.creator.none.fl_str_mv Zachetti, Vanessa Gimena Lourdes
Granero, Adrian Marcelo
Robledo, Sebastian Noel
Zon, María Alicia
Fernandez, Hector
author Zachetti, Vanessa Gimena Lourdes
author_facet Zachetti, Vanessa Gimena Lourdes
Granero, Adrian Marcelo
Robledo, Sebastian Noel
Zon, María Alicia
Fernandez, Hector
author_role author
author2 Granero, Adrian Marcelo
Robledo, Sebastian Noel
Zon, María Alicia
Fernandez, Hector
author2_role author
author
author
author
dc.subject.none.fl_str_mv Electrochemical Biosensor
Citrinin
Multi-Walled Carbon Nanotubes
Peroxidase Enzymes
Mycotoxins
topic Electrochemical Biosensor
Citrinin
Multi-Walled Carbon Nanotubes
Peroxidase Enzymes
Mycotoxins
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An amperometric biosensor based on horseradish peroxidase (EC1.11.1.7,H2O2-oxide-reductases) to determine the content of citrinin mycotoxin in rice samples is proposed by the first time. The method uses carbon paste electrodes filled up with multi-walled carbon nanotubes embedded in a mineral oil, horseradish peroxidase, and ferrocene as a redox mediator. The biosensor is covered externally with a dialysis membrane, which is fixed to the body side of the electrode with a Teflon laboratory film, and an O-ring. The reproducibility and the repeatability were of 7.0% and 3.0%, respectively, showing a very good biosensor performance. The calibration curve was linear in a concentration range from 1 to 11.6 nM. The limits of detection and quantification were 0.25 nM and 0.75 nM, respectively. For comparison, the citrinin content in rice samples was also determined by fluorimetric measurements. A very good correlation was obtained between the electrochemical and spectrophotometric methods.
Fil: Zachetti, Vanessa Gimena Lourdes. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Granero, Adrian Marcelo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Robledo, Sebastian Noel. Universidad Nacional de Rio Cuarto. Facultad de Ingeniería. Departamento de Tecnología Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zon, María Alicia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Hector. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description An amperometric biosensor based on horseradish peroxidase (EC1.11.1.7,H2O2-oxide-reductases) to determine the content of citrinin mycotoxin in rice samples is proposed by the first time. The method uses carbon paste electrodes filled up with multi-walled carbon nanotubes embedded in a mineral oil, horseradish peroxidase, and ferrocene as a redox mediator. The biosensor is covered externally with a dialysis membrane, which is fixed to the body side of the electrode with a Teflon laboratory film, and an O-ring. The reproducibility and the repeatability were of 7.0% and 3.0%, respectively, showing a very good biosensor performance. The calibration curve was linear in a concentration range from 1 to 11.6 nM. The limits of detection and quantification were 0.25 nM and 0.75 nM, respectively. For comparison, the citrinin content in rice samples was also determined by fluorimetric measurements. A very good correlation was obtained between the electrochemical and spectrophotometric methods.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22650
Zachetti, Vanessa Gimena Lourdes; Granero, Adrian Marcelo; Robledo, Sebastian Noel; Zon, María Alicia; Fernandez, Hector; Development of an amperometric biosensor based on peroxidases to quantify citrinin in rice samples; Elsevier Science; Bioelectrochemistry; 91; 1-2013; 37-43
1567-5394
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22650
identifier_str_mv Zachetti, Vanessa Gimena Lourdes; Granero, Adrian Marcelo; Robledo, Sebastian Noel; Zon, María Alicia; Fernandez, Hector; Development of an amperometric biosensor based on peroxidases to quantify citrinin in rice samples; Elsevier Science; Bioelectrochemistry; 91; 1-2013; 37-43
1567-5394
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bioelechem.2012.12.004
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1567539413000042
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782413744635904
score 12.982451

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