Non-covalent functionalization of multi-wall carbon nanotubes with polyarginine: Characterization and analytical applications for uric acid quantification

Autores
Gutierrez, Alejandro; Gutierrez, Fabiana Andrea; Eguílaz Rubio, Marcos; Parrado, Concepción; Rivas, Gustavo Adolfo
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We report for the first time the non-covalent functionalization of multiwall carbon nanotubes (MWCNTs) with polyarginine (Polyarg), the modification of glassy carbon electrodes (GCE) with the resulting Polyarg-MWCNTs dispersion and the analytical application of Polyarg-MWCNTs-modified GCE for the quantification of uric acid. The optimum MWCNT-Polyarg dispersion was obtained by sonicating for 5.0 min the mixture of 0.75 mg mL−1 MWCNTs and 0.50 mg mL−1 Polyarg. The dispersion was characterized by scanning electron microscopy, electrophoretic mobility, electrochemical impedance spectroscopy, cyclic voltammetry and amperometry. The presence of MWCNT-Polyarg at GCE surface produced a drastic decrease in the overvoltages for the oxidation of hydrogen peroxide (300 mV) ascorbic acid (281 mV) and uric acid (70 mV) and for the reduction of hydrogen peroxide (200 mV), as well as an important decrease in the charge transfer resistances for hydrogen peroxide, hydroquinone/quinone and ferricyanide/ferrocyanide markers. The strong adsorption of uric acid at GCE/MWCNT-Polyarg made possible the highly sensitive detection of this biomarker at nanomolar levels even in the presence of 1.0×10−4 M ascorbic acid by Adsorptive Stripping with medium exchange and linear scan voltammetry transduction. The quantification of uric acid in untreated human urine was very successful, demonstrating an excellent correlation (98 %) with the reference method used in clinical laboratories (Uricostat, Wiener Lab).
Fil: Gutierrez, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Gutierrez, Fabiana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Eguílaz Rubio, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Parrado, Concepción. Universidad Complutense de Madrid; España
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
ASCORBIC ACID
CARBON NANOTUBES FUNCTIONALIZATION
ELECTROCHEMICAL SENSOR
POLYARGININE
URIC ACID SENSOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85323
Acceder
id CONICETDig_ea429921088c5874887171ca54915a23
oai_identifier_str oai:ri.conicet.gov.ar:11336/85323
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Non-covalent functionalization of multi-wall carbon nanotubes with polyarginine: Characterization and analytical applications for uric acid quantificationGutierrez, AlejandroGutierrez, Fabiana AndreaEguílaz Rubio, MarcosParrado, ConcepciónRivas, Gustavo AdolfoASCORBIC ACIDCARBON NANOTUBES FUNCTIONALIZATIONELECTROCHEMICAL SENSORPOLYARGININEURIC ACID SENSORhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We report for the first time the non-covalent functionalization of multiwall carbon nanotubes (MWCNTs) with polyarginine (Polyarg), the modification of glassy carbon electrodes (GCE) with the resulting Polyarg-MWCNTs dispersion and the analytical application of Polyarg-MWCNTs-modified GCE for the quantification of uric acid. The optimum MWCNT-Polyarg dispersion was obtained by sonicating for 5.0 min the mixture of 0.75 mg mL−1 MWCNTs and 0.50 mg mL−1 Polyarg. The dispersion was characterized by scanning electron microscopy, electrophoretic mobility, electrochemical impedance spectroscopy, cyclic voltammetry and amperometry. The presence of MWCNT-Polyarg at GCE surface produced a drastic decrease in the overvoltages for the oxidation of hydrogen peroxide (300 mV) ascorbic acid (281 mV) and uric acid (70 mV) and for the reduction of hydrogen peroxide (200 mV), as well as an important decrease in the charge transfer resistances for hydrogen peroxide, hydroquinone/quinone and ferricyanide/ferrocyanide markers. The strong adsorption of uric acid at GCE/MWCNT-Polyarg made possible the highly sensitive detection of this biomarker at nanomolar levels even in the presence of 1.0×10−4 M ascorbic acid by Adsorptive Stripping with medium exchange and linear scan voltammetry transduction. The quantification of uric acid in untreated human urine was very successful, demonstrating an excellent correlation (98 %) with the reference method used in clinical laboratories (Uricostat, Wiener Lab).Fil: Gutierrez, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Gutierrez, Fabiana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Eguílaz Rubio, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Parrado, Concepción. Universidad Complutense de Madrid; EspañaFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaWiley VCH Verlag2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85323Gutierrez, Alejandro; Gutierrez, Fabiana Andrea; Eguílaz Rubio, Marcos; Parrado, Concepción; Rivas, Gustavo Adolfo; Non-covalent functionalization of multi-wall carbon nanotubes with polyarginine: Characterization and analytical applications for uric acid quantification; Wiley VCH Verlag; Electroanalysis; 30; 7; 7-2018; 1416-14241040-03971521-4109CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/elan.201800034info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/elan.201800034info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:14:34Zoai:ri.conicet.gov.ar:11336/85323instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:14:34.781CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Non-covalent functionalization of multi-wall carbon nanotubes with polyarginine: Characterization and analytical applications for uric acid quantification
title Non-covalent functionalization of multi-wall carbon nanotubes with polyarginine: Characterization and analytical applications for uric acid quantification
spellingShingle Non-covalent functionalization of multi-wall carbon nanotubes with polyarginine: Characterization and analytical applications for uric acid quantification
Gutierrez, Alejandro
ASCORBIC ACID
CARBON NANOTUBES FUNCTIONALIZATION
ELECTROCHEMICAL SENSOR
POLYARGININE
URIC ACID SENSOR
title_short Non-covalent functionalization of multi-wall carbon nanotubes with polyarginine: Characterization and analytical applications for uric acid quantification
title_full Non-covalent functionalization of multi-wall carbon nanotubes with polyarginine: Characterization and analytical applications for uric acid quantification
title_fullStr Non-covalent functionalization of multi-wall carbon nanotubes with polyarginine: Characterization and analytical applications for uric acid quantification
title_full_unstemmed Non-covalent functionalization of multi-wall carbon nanotubes with polyarginine: Characterization and analytical applications for uric acid quantification
title_sort Non-covalent functionalization of multi-wall carbon nanotubes with polyarginine: Characterization and analytical applications for uric acid quantification
dc.creator.none.fl_str_mv Gutierrez, Alejandro
Gutierrez, Fabiana Andrea
Eguílaz Rubio, Marcos
Parrado, Concepción
Rivas, Gustavo Adolfo
author Gutierrez, Alejandro
author_facet Gutierrez, Alejandro
Gutierrez, Fabiana Andrea
Eguílaz Rubio, Marcos
Parrado, Concepción
Rivas, Gustavo Adolfo
author_role author
author2 Gutierrez, Fabiana Andrea
Eguílaz Rubio, Marcos
Parrado, Concepción
Rivas, Gustavo Adolfo
author2_role author
author
author
author
dc.subject.none.fl_str_mv ASCORBIC ACID
CARBON NANOTUBES FUNCTIONALIZATION
ELECTROCHEMICAL SENSOR
POLYARGININE
URIC ACID SENSOR
topic ASCORBIC ACID
CARBON NANOTUBES FUNCTIONALIZATION
ELECTROCHEMICAL SENSOR
POLYARGININE
URIC ACID SENSOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We report for the first time the non-covalent functionalization of multiwall carbon nanotubes (MWCNTs) with polyarginine (Polyarg), the modification of glassy carbon electrodes (GCE) with the resulting Polyarg-MWCNTs dispersion and the analytical application of Polyarg-MWCNTs-modified GCE for the quantification of uric acid. The optimum MWCNT-Polyarg dispersion was obtained by sonicating for 5.0 min the mixture of 0.75 mg mL−1 MWCNTs and 0.50 mg mL−1 Polyarg. The dispersion was characterized by scanning electron microscopy, electrophoretic mobility, electrochemical impedance spectroscopy, cyclic voltammetry and amperometry. The presence of MWCNT-Polyarg at GCE surface produced a drastic decrease in the overvoltages for the oxidation of hydrogen peroxide (300 mV) ascorbic acid (281 mV) and uric acid (70 mV) and for the reduction of hydrogen peroxide (200 mV), as well as an important decrease in the charge transfer resistances for hydrogen peroxide, hydroquinone/quinone and ferricyanide/ferrocyanide markers. The strong adsorption of uric acid at GCE/MWCNT-Polyarg made possible the highly sensitive detection of this biomarker at nanomolar levels even in the presence of 1.0×10−4 M ascorbic acid by Adsorptive Stripping with medium exchange and linear scan voltammetry transduction. The quantification of uric acid in untreated human urine was very successful, demonstrating an excellent correlation (98 %) with the reference method used in clinical laboratories (Uricostat, Wiener Lab).
Fil: Gutierrez, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Gutierrez, Fabiana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Eguílaz Rubio, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Parrado, Concepción. Universidad Complutense de Madrid; España
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description We report for the first time the non-covalent functionalization of multiwall carbon nanotubes (MWCNTs) with polyarginine (Polyarg), the modification of glassy carbon electrodes (GCE) with the resulting Polyarg-MWCNTs dispersion and the analytical application of Polyarg-MWCNTs-modified GCE for the quantification of uric acid. The optimum MWCNT-Polyarg dispersion was obtained by sonicating for 5.0 min the mixture of 0.75 mg mL−1 MWCNTs and 0.50 mg mL−1 Polyarg. The dispersion was characterized by scanning electron microscopy, electrophoretic mobility, electrochemical impedance spectroscopy, cyclic voltammetry and amperometry. The presence of MWCNT-Polyarg at GCE surface produced a drastic decrease in the overvoltages for the oxidation of hydrogen peroxide (300 mV) ascorbic acid (281 mV) and uric acid (70 mV) and for the reduction of hydrogen peroxide (200 mV), as well as an important decrease in the charge transfer resistances for hydrogen peroxide, hydroquinone/quinone and ferricyanide/ferrocyanide markers. The strong adsorption of uric acid at GCE/MWCNT-Polyarg made possible the highly sensitive detection of this biomarker at nanomolar levels even in the presence of 1.0×10−4 M ascorbic acid by Adsorptive Stripping with medium exchange and linear scan voltammetry transduction. The quantification of uric acid in untreated human urine was very successful, demonstrating an excellent correlation (98 %) with the reference method used in clinical laboratories (Uricostat, Wiener Lab).
publishDate 2018
dc.date.none.fl_str_mv 2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85323
Gutierrez, Alejandro; Gutierrez, Fabiana Andrea; Eguílaz Rubio, Marcos; Parrado, Concepción; Rivas, Gustavo Adolfo; Non-covalent functionalization of multi-wall carbon nanotubes with polyarginine: Characterization and analytical applications for uric acid quantification; Wiley VCH Verlag; Electroanalysis; 30; 7; 7-2018; 1416-1424
1040-0397
1521-4109
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85323
identifier_str_mv Gutierrez, Alejandro; Gutierrez, Fabiana Andrea; Eguílaz Rubio, Marcos; Parrado, Concepción; Rivas, Gustavo Adolfo; Non-covalent functionalization of multi-wall carbon nanotubes with polyarginine: Characterization and analytical applications for uric acid quantification; Wiley VCH Verlag; Electroanalysis; 30; 7; 7-2018; 1416-1424
1040-0397
1521-4109
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/elan.201800034
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1002/elan.201800034
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781567991545856
score 12.982451

Publicaciones similares